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The map label for this gene is polA [H]

Identifier: 158425221

GI number: 158425221

Start: 4127616

End: 4129445

Strand: Reverse

Name: polA [H]

Synonym: AZC_3597

Alternate gene names: 158425221

Gene position: 4129445-4127616 (Counterclockwise)

Preceding gene: 158425222

Following gene: 158425220

Centisome position: 76.9

GC content: 61.58

Gene sequence:

>1830_bases
ATGAGCCATCTGATATTCGACATCGAGACCAACGGGCTGTTGGACGCGACGAACCGCGTTCACTGCCTCTGCATCAAGGA
CGCTGACACGGGGCAGGTCTGGTCCTGCACCGACGACGGTCCCACCGGAACCTACATCCCTATTGAGCGTGGACTTCAGC
TCCTCGCGGATGCCGAGGTTTTGATCGGCCACAACATCCTCAAGTTCGACATCCCCGCGCTTCAGAAGGTCTATCCGTGG
TGGAAGCCCAAGGGCGTGCTGCGGGACACCATCGTCTGTTCTCGGCTGATCTGGCCGAAGGACGACCTGCGGGAGCGGGA
CTTCCGGCTTCAGAAGCAGGGCAAGCTTCCCGGCAACCTCATCGGCCGCTACGCCTTGGAGGCGTGGGGCTATCGCCTCG
GGGAGTACAAGGGCGACTACAAGGGACCTTGGGACGTCTGGTCCCAAGAGATGCAGGATTACTGTGAGCAGGACGTTGAG
GTCACCTTCCGCCTTTACCAGAAGATCAAGGAAAAGGGCTGGAAAGAGGAGTCCATTGAGCTTGAACACCGGGTTCAGTG
GATCATCCATCGGCAGGAGCAGTACGGCTTTCTGTTCGATCAGGCCAAGGCCGCGTCCCTCTATGCCGACCTTGTCCAGC
GCAAGACCGAGCTAGAGGCCGAGCTTCAGTCCGCCTTCCCGCCGTGGGAGGTCGAGAGCGTCTTCGTTCCCAAGGTGAAC
AACCGGGTGCGCGGCTACGTGAAGGGCGTTCCTTTCACCAAGCGGCGGACTATTCACTTCAAGCCGTCAAGCCGCGACCA
CATCGCGGGACGCCTCAAGGCCAAGTACGGCTGGGAGCCCTCCGAGTTCACCGACGAGGGCAAACCCAAGGTGGACGAAG
ATGTCCTTTCGCATCTTCCGTACCCTGAGGCCAAGCTTCTCTCCGAATATCTCATGGTCGAGAAGCGGCTGGGGCAGATC
GCCACCGGCAAGGAAGCGTGGCTGAAGCACGTCAAGGCGGACGGACGCATCCACGGCGACGTGACCACCAACGGCGCGGT
GACGGGTCGCATGACCCATTCCAAGCCGAACATCGCGCAGGTGCCGGGCAACGGCTCCCCCTATGGGCACCGCTGCCGGG
AGCTGTTCGTTGCCGCCAAACGCAAGCTTCTGGTGGGCTGCGATGCCGACGCCTTGGAGCTGCGTTGCCTCGCCGGCTAC
ATGGCCCGGTACGACGGCGGCGCTTATGTCCGCACCGTCCTTGAAGGGAAGAAGGAACTCGGGACCGACATGCACACCCT
CAACGCGAAGGCGTTGGGGTGCAGCCGCGATGTCGCGAAGGTCTGGTTCTACGCCTTCATTTATGGGGCAGGGGACTTCA
AGCTCGGGACCATCCTCGGTGCCCCCAAGGGCGAGGAGCAGAAGTGGGGCCGGCGGTCCCGTGCCCGCTTCCTCAAGGCC
CTGCCGGCGCTCGGAACCATCATCAAGAAGGTGCAGGAGAAGGTTCAGAAAAAGGGCTTTCTCCGGGGGCTGGACGGTCG
GGAACTGCGGGTTCGTTCCGCGCACTCGGCGTTCAACACCCTCCTTCAGTCGGCGGGCGCGGTCCTCATGAAGATGGCTC
TTGTCCTCTTGGACGAGGACCTTCAGGCCGCAGGCTATGTGCCCGGCCAGCATTATGAGTTCGTCGCCAACGTCCACGAC
GAATGGCAGATCGAAGTAAACGAGGAGATTGCCGAGGATGTCGGCAAACGTGCAGTCACTGCAATTGTCCGAGCCGGAGA
ACACTTCGGGTTCGGATGCCCTCTCAGTGGCAATTTCGCAGTCGGTCGCTCTTGGGCCGAAACTCACTGA

Upstream 100 bases:

>100_bases
GAGGAGGAGGATGACGACCCCCCGTTCTGATTGACCACCCGCACGACCGGGCTGGGTCCGGTGCAGTCACTGCACCGTCA
GGCCACTCTCAGGGAGGGCG

Downstream 100 bases:

>100_bases
GATCGTGCAGGAAGCTTGGCTCAAGCCCTTCATGACCAAGAGCAATATCGCCAAGGCCGAAGCGGACGGGGTCGCACTTG
CGGCCTCGCTCGGCCTCATC

Product: DNA polymerase

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 609; Mature: 608

Protein sequence:

>609_residues
MSHLIFDIETNGLLDATNRVHCLCIKDADTGQVWSCTDDGPTGTYIPIERGLQLLADAEVLIGHNILKFDIPALQKVYPW
WKPKGVLRDTIVCSRLIWPKDDLRERDFRLQKQGKLPGNLIGRYALEAWGYRLGEYKGDYKGPWDVWSQEMQDYCEQDVE
VTFRLYQKIKEKGWKEESIELEHRVQWIIHRQEQYGFLFDQAKAASLYADLVQRKTELEAELQSAFPPWEVESVFVPKVN
NRVRGYVKGVPFTKRRTIHFKPSSRDHIAGRLKAKYGWEPSEFTDEGKPKVDEDVLSHLPYPEAKLLSEYLMVEKRLGQI
ATGKEAWLKHVKADGRIHGDVTTNGAVTGRMTHSKPNIAQVPGNGSPYGHRCRELFVAAKRKLLVGCDADALELRCLAGY
MARYDGGAYVRTVLEGKKELGTDMHTLNAKALGCSRDVAKVWFYAFIYGAGDFKLGTILGAPKGEEQKWGRRSRARFLKA
LPALGTIIKKVQEKVQKKGFLRGLDGRELRVRSAHSAFNTLLQSAGAVLMKMALVLLDEDLQAAGYVPGQHYEFVANVHD
EWQIEVNEEIAEDVGKRAVTAIVRAGEHFGFGCPLSGNFAVGRSWAETH

Sequences:

>Translated_609_residues
MSHLIFDIETNGLLDATNRVHCLCIKDADTGQVWSCTDDGPTGTYIPIERGLQLLADAEVLIGHNILKFDIPALQKVYPW
WKPKGVLRDTIVCSRLIWPKDDLRERDFRLQKQGKLPGNLIGRYALEAWGYRLGEYKGDYKGPWDVWSQEMQDYCEQDVE
VTFRLYQKIKEKGWKEESIELEHRVQWIIHRQEQYGFLFDQAKAASLYADLVQRKTELEAELQSAFPPWEVESVFVPKVN
NRVRGYVKGVPFTKRRTIHFKPSSRDHIAGRLKAKYGWEPSEFTDEGKPKVDEDVLSHLPYPEAKLLSEYLMVEKRLGQI
ATGKEAWLKHVKADGRIHGDVTTNGAVTGRMTHSKPNIAQVPGNGSPYGHRCRELFVAAKRKLLVGCDADALELRCLAGY
MARYDGGAYVRTVLEGKKELGTDMHTLNAKALGCSRDVAKVWFYAFIYGAGDFKLGTILGAPKGEEQKWGRRSRARFLKA
LPALGTIIKKVQEKVQKKGFLRGLDGRELRVRSAHSAFNTLLQSAGAVLMKMALVLLDEDLQAAGYVPGQHYEFVANVHD
EWQIEVNEEIAEDVGKRAVTAIVRAGEHFGFGCPLSGNFAVGRSWAETH
>Mature_608_residues
SHLIFDIETNGLLDATNRVHCLCIKDADTGQVWSCTDDGPTGTYIPIERGLQLLADAEVLIGHNILKFDIPALQKVYPWW
KPKGVLRDTIVCSRLIWPKDDLRERDFRLQKQGKLPGNLIGRYALEAWGYRLGEYKGDYKGPWDVWSQEMQDYCEQDVEV
TFRLYQKIKEKGWKEESIELEHRVQWIIHRQEQYGFLFDQAKAASLYADLVQRKTELEAELQSAFPPWEVESVFVPKVNN
RVRGYVKGVPFTKRRTIHFKPSSRDHIAGRLKAKYGWEPSEFTDEGKPKVDEDVLSHLPYPEAKLLSEYLMVEKRLGQIA
TGKEAWLKHVKADGRIHGDVTTNGAVTGRMTHSKPNIAQVPGNGSPYGHRCRELFVAAKRKLLVGCDADALELRCLAGYM
ARYDGGAYVRTVLEGKKELGTDMHTLNAKALGCSRDVAKVWFYAFIYGAGDFKLGTILGAPKGEEQKWGRRSRARFLKAL
PALGTIIKKVQEKVQKKGFLRGLDGRELRVRSAHSAFNTLLQSAGAVLMKMALVLLDEDLQAAGYVPGQHYEFVANVHDE
WQIEVNEEIAEDVGKRAVTAIVRAGEHFGFGCPLSGNFAVGRSWAETH

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity. It is able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1790294, Length=488, Percent_Identity=23.9754098360656, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01612 3_5_exonuc; PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 68846; Mature: 68714

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHLIFDIETNGLLDATNRVHCLCIKDADTGQVWSCTDDGPTGTYIPIERGLQLLADAEV
CCCEEEEEECCCEEECCCCEEEEEEECCCCCCEEEECCCCCCCCEEEHHHCHHHHHCCHH
LIGHNILKFDIPALQKVYPWWKPKGVLRDTIVCSRLIWPKDDLRERDFRLQKQGKLPGNL
HHCCCEEEECCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHH
IGRYALEAWGYRLGEYKGDYKGPWDVWSQEMQDYCEQDVEVTFRLYQKIKEKGWKEESIE
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
LEHRVQWIIHRQEQYGFLFDQAKAASLYADLVQRKTELEAELQSAFPPWEVESVFVPKVN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCC
NRVRGYVKGVPFTKRRTIHFKPSSRDHIAGRLKAKYGWEPSEFTDEGKPKVDEDVLSHLP
CCHHHEECCCCCCCCEEEEECCCCCCHHHHHEECCCCCCCCCCCCCCCCCCCHHHHHHCC
YPEAKLLSEYLMVEKRLGQIATGKEAWLKHVKADGRIHGDVTTNGAVTGRMTHSKPNIAQ
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEECCEECCCEEEEEEECCCCCEEE
VPGNGSPYGHRCRELFVAAKRKLLVGCDADALELRCLAGYMARYDGGAYVRTVLEGKKEL
CCCCCCCHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GTDMHTLNAKALGCSRDVAKVWFYAFIYGAGDFKLGTILGAPKGEEQKWGRRSRARFLKA
CCCHHHCCCHHCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHH
LPALGTIIKKVQEKVQKKGFLRGLDGRELRVRSAHSAFNTLLQSAGAVLMKMALVLLDED
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LQAAGYVPGQHYEFVANVHDEWQIEVNEEIAEDVGKRAVTAIVRAGEHFGFGCPLSGNFA
HHHCCCCCCCCHHEEECCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
VGRSWAETH
CCCCCCCCC
>Mature Secondary Structure 
SHLIFDIETNGLLDATNRVHCLCIKDADTGQVWSCTDDGPTGTYIPIERGLQLLADAEV
CCEEEEEECCCEEECCCCEEEEEEECCCCCCEEEECCCCCCCCEEEHHHCHHHHHCCHH
LIGHNILKFDIPALQKVYPWWKPKGVLRDTIVCSRLIWPKDDLRERDFRLQKQGKLPGNL
HHCCCEEEECCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHH
IGRYALEAWGYRLGEYKGDYKGPWDVWSQEMQDYCEQDVEVTFRLYQKIKEKGWKEESIE
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
LEHRVQWIIHRQEQYGFLFDQAKAASLYADLVQRKTELEAELQSAFPPWEVESVFVPKVN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCC
NRVRGYVKGVPFTKRRTIHFKPSSRDHIAGRLKAKYGWEPSEFTDEGKPKVDEDVLSHLP
CCHHHEECCCCCCCCEEEEECCCCCCHHHHHEECCCCCCCCCCCCCCCCCCCHHHHHHCC
YPEAKLLSEYLMVEKRLGQIATGKEAWLKHVKADGRIHGDVTTNGAVTGRMTHSKPNIAQ
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEECCEECCCEEEEEEECCCCCEEE
VPGNGSPYGHRCRELFVAAKRKLLVGCDADALELRCLAGYMARYDGGAYVRTVLEGKKEL
CCCCCCCHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GTDMHTLNAKALGCSRDVAKVWFYAFIYGAGDFKLGTILGAPKGEEQKWGRRSRARFLKA
CCCHHHCCCHHCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHH
LPALGTIIKKVQEKVQKKGFLRGLDGRELRVRSAHSAFNTLLQSAGAVLMKMALVLLDED
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LQAAGYVPGQHYEFVANVHDEWQIEVNEEIAEDVGKRAVTAIVRAGEHFGFGCPLSGNFA
HHHCCCCCCCCHHEEECCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
VGRSWAETH
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6276402; 8346018; 9278503; 6183253; 6302278; 7035456; 3883192; 1989886; 8469987; 8260491; 9514742; 9888810; 10588690; 8442659 [H]