Click here to switch to the map view.

The map label for this gene is thiG

Identifier: 158425176

GI number: 158425176

Start: 4080561

End: 4081361

Strand: Reverse

Name: thiG

Synonym: AZC_3552

Alternate gene names: 158425176

Gene position: 4081361-4080561 (Counterclockwise)

Preceding gene: 158425177

Following gene: 158425171

Centisome position: 76.01

GC content: 67.29

Gene sequence:

>801_bases
ATGAACGTGAACGTTCCCGTCCCGAGCACGCTGGCCGATCCGCTGGTGATCGCCGGCCGCACCTTCTCCTCCCGCCTGTT
CCTCGGCACGGCGGGCTATCCCAACCAGAAGGTGTTTCTCGATGCGCTGGAGGCCTCCGGTTCGGAAATGGCCACCGCCT
CCATCCGCCGCATGAGCCTTGAGGGCTATGAGGAGAGCCTTGCGGACCTTCTTACCGGCCGCGTGCACATCCTGCCCAAC
ACTGCCGGCTGCCAGACCGCCAAGGACGCCATCCTCACCGCCGAACTGGCGCGCGAGGCGCTGGAGACGGACTGGGTGAA
GCTGGAGGTGATCGGCGACCGCGAGCTGCTCTATCCCAACGTGGAAGAGCTGCTGAAGGCCACCGAGGAACTGGTGAACC
GGGGCTTCGTGGTGCTGCCCTATTGCAATGACGATCCTGTCACCTGCCAGAAGCTGGCGGATCTCGGCGCCGCCACCGTG
ATGCCGCTCGGTTCCATGATCGGCACCGGCCTTGGCATTGCCAATCCGCACATGATCGAGCTGATCTGCGCCCGCTCGCC
GGTGCCCGTCGTGCTGGATGCCGGCATCGGCACCGCGTCCGACGCCGCGCAGGCCATGGAGCTCGGCTGTGCTGCGGTGC
TGCTCAATACGGCGGTGTCCAAGGCCCACGATCCGGTGCGCATGGCCCGCGCCTTCCGCTACGCGGTGGAAGGCGGGCGT
CTGGCGCGCCTCGCCGGCCGCATCCCGAAGAAGCTGCACGCGGAAGCCTCCAGCCCCGAATTCGGCCTCGTCGGCAGCTG
A

Upstream 100 bases:

>100_bases
GGCGATGCGGTGGAGATCATCCACGCCAAGCAGGGCGGCTGACCGCATCAGCCCCGCGAACAATCTGAGCGCCGGATCAG
CCGGCCGGAAAGAGACCGAC

Downstream 100 bases:

>100_bases
TCCCAGCCATCTGCCGGGTCCCTCGGGACCCGGCATCGCGTTTCAGTCCTCGAACTTCTCTGTGGCGTCGGCGGTGCCGT
GCACCCAATAGCCCGATGCC

Product: thiamin biosynthesis protein

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MNVNVPVPSTLADPLVIAGRTFSSRLFLGTAGYPNQKVFLDALEASGSEMATASIRRMSLEGYEESLADLLTGRVHILPN
TAGCQTAKDAILTAELAREALETDWVKLEVIGDRELLYPNVEELLKATEELVNRGFVVLPYCNDDPVTCQKLADLGAATV
MPLGSMIGTGLGIANPHMIELICARSPVPVVLDAGIGTASDAAQAMELGCAAVLLNTAVSKAHDPVRMARAFRYAVEGGR
LARLAGRIPKKLHAEASSPEFGLVGS

Sequences:

>Translated_266_residues
MNVNVPVPSTLADPLVIAGRTFSSRLFLGTAGYPNQKVFLDALEASGSEMATASIRRMSLEGYEESLADLLTGRVHILPN
TAGCQTAKDAILTAELAREALETDWVKLEVIGDRELLYPNVEELLKATEELVNRGFVVLPYCNDDPVTCQKLADLGAATV
MPLGSMIGTGLGIANPHMIELICARSPVPVVLDAGIGTASDAAQAMELGCAAVLLNTAVSKAHDPVRMARAFRYAVEGGR
LARLAGRIPKKLHAEASSPEFGLVGS
>Mature_266_residues
MNVNVPVPSTLADPLVIAGRTFSSRLFLGTAGYPNQKVFLDALEASGSEMATASIRRMSLEGYEESLADLLTGRVHILPN
TAGCQTAKDAILTAELAREALETDWVKLEVIGDRELLYPNVEELLKATEELVNRGFVVLPYCNDDPVTCQKLADLGAATV
MPLGSMIGTGLGIANPHMIELICARSPVPVVLDAGIGTASDAAQAMELGCAAVLLNTAVSKAHDPVRMARAFRYAVEGGR
LARLAGRIPKKLHAEASSPEFGLVGS

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=251, Percent_Identity=48.207171314741, Blast_Score=234, Evalue=5e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_AZOC5 (A8IEY6)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001526468.1
- ProteinModelPortal:   A8IEY6
- SMR:   A8IEY6
- GeneID:   5691129
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_3552
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   CLSK971243
- BioCyc:   ACAU438753:AZC_3552-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 28126; Mature: 28126

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: ACT_SITE 107-107 BINDING 168-168

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVNVPVPSTLADPLVIAGRTFSSRLFLGTAGYPNQKVFLDALEASGSEMATASIRRMSL
CCCCCCCCCHHCCCEEEECCCCCCEEEEEECCCCCCCEEEEHHHCCCCHHHHHHHHHHHH
EGYEESLADLLTGRVHILPNTAGCQTAKDAILTAELAREALETDWVKLEVIGDRELLYPN
HHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCC
VEELLKATEELVNRGFVVLPYCNDDPVTCQKLADLGAATVMPLGSMIGTGLGIANPHMIE
HHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHEE
LICARSPVPVVLDAGIGTASDAAQAMELGCAAVLLNTAVSKAHDPVRMARAFRYAVEGGR
EEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCH
LARLAGRIPKKLHAEASSPEFGLVGS
HHHHHHHCCHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNVNVPVPSTLADPLVIAGRTFSSRLFLGTAGYPNQKVFLDALEASGSEMATASIRRMSL
CCCCCCCCCHHCCCEEEECCCCCCEEEEEECCCCCCCEEEEHHHCCCCHHHHHHHHHHHH
EGYEESLADLLTGRVHILPNTAGCQTAKDAILTAELAREALETDWVKLEVIGDRELLYPN
HHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCC
VEELLKATEELVNRGFVVLPYCNDDPVTCQKLADLGAATVMPLGSMIGTGLGIANPHMIE
HHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHEE
LICARSPVPVVLDAGIGTASDAAQAMELGCAAVLLNTAVSKAHDPVRMARAFRYAVEGGR
EEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCH
LARLAGRIPKKLHAEASSPEFGLVGS
HHHHHHHCCHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA