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The map label for this gene is stp [H]

Identifier: 158425146

GI number: 158425146

Start: 4046015

End: 4047685

Strand: Reverse

Name: stp [H]

Synonym: AZC_3522

Alternate gene names: 158425146

Gene position: 4047685-4046015 (Counterclockwise)

Preceding gene: 158425147

Following gene: 158425145

Centisome position: 75.38

GC content: 70.14

Gene sequence:

>1671_bases
TTGACCCCACCGCCCCGCAGCCCTGTACAAGCTGGGCCGCGCGCGGGACAATTGGCCTCATGTTACCGTTTTGACATGTA
TTCCGCTGTCAGGACGGCCGTTCACATGAGGGCTCGCGGGATGGGCGTCGGGTCGATCGACAGCCGGCAGCGCTGGTGCA
CCGTCTTCGCGGCGGCTGGCGCGCTCGGCATGATCATGATCGACGCCACCGGCACCGCCGTGGCGCTGCCCTCCATCCAG
CGGGACCTGGTGCTGAGCCATGGCGCCCAGCAATGGATCATCACCCTCTACGCCCTCACCGTGGCGGTGGCCATCGCCAC
AGGTGGCCGGCTGGCCGATGTCTATGGCCGTGCGCGGGTTTTCCGCGTCGGTGTGATGCTGTTCGCGTTCGGCTCAGTGG
TGAGCGGGCTTGCGGTCAATCTGCCCATGCTGCTGTGCGGGCGCACCCTTGAAGGACTCGGCAACATCCTGATGGCGCCG
GCGGCGGCGCTGCTCGCCACCGAGGCGTTCGGCCCCACGGAGCGCGGGCGGGCGATGGGGCTTTACAGCGGCCTCGGCGG
GCTTGCCATGGTCATCGGCCCCATCGTCTGCGGGGCGGTGGTGCAGGTGGGCGGCTGGCGCTGGGCCTTCTTCGTCAATC
TGCCGCTGGCCCTTGCCGTCCTGCTTCTGCTGCGCGCTGCGGGCCCGGCCGCCGCGAGCCCGCGCAGCGGTACCTTCCGG
CCCGCACACAGCCTGCTTCTGGTGGCGGCGCTCGGCCCCATCGTGCTGGGTCTCCAGGAGAGCCATGCCTGGGGCTGGAC
CTCGCCGCTCACGCTCTCGCTGATCGCCATCGGGGCTTTGCTGCTGGTGCTGTTCGTGGCCACGCAGGCGCGGGCGGCGG
ACCCCTTGGTGGATGTGCGCCTGTTCACACGGCGGCAGTTCTGTGCCGATGGCCTTGTCCTGTTCTGCGCCCAGTGTGCC
ATCATCGGGCAGTCGGCCTATGGCGCCATCTATCTGCAACGGGTGCTGCATTTCGCGCCGCTGCAATCCGGCCTCGCCAT
GCTGCTGTTCCTCGGCCCCATGATGGTGTGCGCCCCGCTCTCGGGCGTGCTGTATGACCGCCATGGCGTGAAGGTGCCGG
TGGTGGCGGGCCTCACGCTGGCGACCCTCGGCTTCGTGCTGGAGGTTCATGCGCTGCCGCGCATGGATTTCCTCTATATG
GCGCCTGCCCTGGTGATGCTGGGTGCCGGCATGGGCCTCGCCCTGTCGCAGACCTATACGGACGGCACCGCGCAGGTCGC
GGAAAGCCACCGCGGCCGGGCCTTCGGCGCCCTCGATACGGTGCGCCAGCTCGGCGGCGCCATCGGCATGGCGGCCATCG
GCACCGTCGTTGTGGCGCTGGAGCGCGGCCGTGTCCTTGCCATCGCGGCAGAAGCGGCCCCGGAGGGGGCGGCGCGGGCG
CAGCTCGAAGCCCTCATGGCCGACGCCGCCTATGGCAAGGACGAGGCGGTACGCACGCTGGCGGAGCAGTGGCCAGCGGT
TGGTGCGGCCCTGCGGCTCAGCGCCGCCCGAAGCATCTCTGAGGGCTATTACGTGGGAGCTGCAATGCTGGCGCTCGCCC
TGTGCGCCGCCGTGCTGGTGATGCGCCGCCCGCCGCACGCGCCCCGCGAGATCGCCAAGGGAGCCTCCTGA

Upstream 100 bases:

>100_bases
GTCCATGCTGAAATACGCGGTCTAGGAGCCGCCCGCTCTGGCATCTGCAAGTCGTAGAGGGCGTCTCCGGCCGGTGCCAT
GCCTGGAGACCCGGCGATAA

Downstream 100 bases:

>100_bases
TGCGCATCCTCTTCGCTTCCCTCGGATCGCTGGGCGATCTCTTTCCCATGCTCGCCATGGCGCAGGCCCTGCGGGCGCGG
GGGCACACACCGGCCATTGC

Product: hypothetical protein

Products: NA

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MTPPPRSPVQAGPRAGQLASCYRFDMYSAVRTAVHMRARGMGVGSIDSRQRWCTVFAAAGALGMIMIDATGTAVALPSIQ
RDLVLSHGAQQWIITLYALTVAVAIATGGRLADVYGRARVFRVGVMLFAFGSVVSGLAVNLPMLLCGRTLEGLGNILMAP
AAALLATEAFGPTERGRAMGLYSGLGGLAMVIGPIVCGAVVQVGGWRWAFFVNLPLALAVLLLLRAAGPAAASPRSGTFR
PAHSLLLVAALGPIVLGLQESHAWGWTSPLTLSLIAIGALLLVLFVATQARAADPLVDVRLFTRRQFCADGLVLFCAQCA
IIGQSAYGAIYLQRVLHFAPLQSGLAMLLFLGPMMVCAPLSGVLYDRHGVKVPVVAGLTLATLGFVLEVHALPRMDFLYM
APALVMLGAGMGLALSQTYTDGTAQVAESHRGRAFGALDTVRQLGGAIGMAAIGTVVVALERGRVLAIAAEAAPEGAARA
QLEALMADAAYGKDEAVRTLAEQWPAVGAALRLSAARSISEGYYVGAAMLALALCAAVLVMRRPPHAPREIAKGAS

Sequences:

>Translated_556_residues
MTPPPRSPVQAGPRAGQLASCYRFDMYSAVRTAVHMRARGMGVGSIDSRQRWCTVFAAAGALGMIMIDATGTAVALPSIQ
RDLVLSHGAQQWIITLYALTVAVAIATGGRLADVYGRARVFRVGVMLFAFGSVVSGLAVNLPMLLCGRTLEGLGNILMAP
AAALLATEAFGPTERGRAMGLYSGLGGLAMVIGPIVCGAVVQVGGWRWAFFVNLPLALAVLLLLRAAGPAAASPRSGTFR
PAHSLLLVAALGPIVLGLQESHAWGWTSPLTLSLIAIGALLLVLFVATQARAADPLVDVRLFTRRQFCADGLVLFCAQCA
IIGQSAYGAIYLQRVLHFAPLQSGLAMLLFLGPMMVCAPLSGVLYDRHGVKVPVVAGLTLATLGFVLEVHALPRMDFLYM
APALVMLGAGMGLALSQTYTDGTAQVAESHRGRAFGALDTVRQLGGAIGMAAIGTVVVALERGRVLAIAAEAAPEGAARA
QLEALMADAAYGKDEAVRTLAEQWPAVGAALRLSAARSISEGYYVGAAMLALALCAAVLVMRRPPHAPREIAKGAS
>Mature_555_residues
TPPPRSPVQAGPRAGQLASCYRFDMYSAVRTAVHMRARGMGVGSIDSRQRWCTVFAAAGALGMIMIDATGTAVALPSIQR
DLVLSHGAQQWIITLYALTVAVAIATGGRLADVYGRARVFRVGVMLFAFGSVVSGLAVNLPMLLCGRTLEGLGNILMAPA
AALLATEAFGPTERGRAMGLYSGLGGLAMVIGPIVCGAVVQVGGWRWAFFVNLPLALAVLLLLRAAGPAAASPRSGTFRP
AHSLLLVAALGPIVLGLQESHAWGWTSPLTLSLIAIGALLLVLFVATQARAADPLVDVRLFTRRQFCADGLVLFCAQCAI
IGQSAYGAIYLQRVLHFAPLQSGLAMLLFLGPMMVCAPLSGVLYDRHGVKVPVVAGLTLATLGFVLEVHALPRMDFLYMA
PALVMLGAGMGLALSQTYTDGTAQVAESHRGRAFGALDTVRQLGGAIGMAAIGTVVVALERGRVLAIAAEAAPEGAARAQ
LEALMADAAYGKDEAVRTLAEQWPAVGAALRLSAARSISEGYYVGAAMLALALCAAVLVMRRPPHAPREIAKGAS

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=419, Percent_Identity=26.7303102625298, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1788710, Length=515, Percent_Identity=23.495145631068, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1790195, Length=256, Percent_Identity=32.03125, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789042, Length=285, Percent_Identity=26.6666666666667, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1788392, Length=345, Percent_Identity=28.9855072463768, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324954, Length=400, Percent_Identity=22.25, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322958, Length=513, Percent_Identity=20.6627680311891, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6325455, Length=151, Percent_Identity=28.476821192053, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=131, Percent_Identity=29.0076335877863, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57812; Mature: 57681

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPPPRSPVQAGPRAGQLASCYRFDMYSAVRTAVHMRARGMGVGSIDSRQRWCTVFAAAG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHC
ALGMIMIDATGTAVALPSIQRDLVLSHGAQQWIITLYALTVAVAIATGGRLADVYGRARV
CCEEEEEECCCCEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
FRVGVMLFAFGSVVSGLAVNLPMLLCGRTLEGLGNILMAPAAALLATEAFGPTERGRAMG
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
LYSGLGGLAMVIGPIVCGAVVQVGGWRWAFFVNLPLALAVLLLLRAAGPAAASPRSGTFR
HHHCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCC
PAHSLLLVAALGPIVLGLQESHAWGWTSPLTLSLIAIGALLLVLFVATQARAADPLVDVR
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LFTRRQFCADGLVLFCAQCAIIGQSAYGAIYLQRVLHFAPLQSGLAMLLFLGPMMVCAPL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGVLYDRHGVKVPVVAGLTLATLGFVLEVHALPRMDFLYMAPALVMLGAGMGLALSQTYT
HHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEECCCC
DGTAQVAESHRGRAFGALDTVRQLGGAIGMAAIGTVVVALERGRVLAIAAEAAPEGAARA
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHH
QLEALMADAAYGKDEAVRTLAEQWPAVGAALRLSAARSISEGYYVGAAMLALALCAAVLV
HHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
MRRPPHAPREIAKGAS
HCCCCCCHHHHHCCCC
>Mature Secondary Structure 
TPPPRSPVQAGPRAGQLASCYRFDMYSAVRTAVHMRARGMGVGSIDSRQRWCTVFAAAG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHC
ALGMIMIDATGTAVALPSIQRDLVLSHGAQQWIITLYALTVAVAIATGGRLADVYGRARV
CCEEEEEECCCCEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
FRVGVMLFAFGSVVSGLAVNLPMLLCGRTLEGLGNILMAPAAALLATEAFGPTERGRAMG
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
LYSGLGGLAMVIGPIVCGAVVQVGGWRWAFFVNLPLALAVLLLLRAAGPAAASPRSGTFR
HHHCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCC
PAHSLLLVAALGPIVLGLQESHAWGWTSPLTLSLIAIGALLLVLFVATQARAADPLVDVR
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LFTRRQFCADGLVLFCAQCAIIGQSAYGAIYLQRVLHFAPLQSGLAMLLFLGPMMVCAPL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGVLYDRHGVKVPVVAGLTLATLGFVLEVHALPRMDFLYMAPALVMLGAGMGLALSQTYT
HHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEECCCC
DGTAQVAESHRGRAFGALDTVRQLGGAIGMAAIGTVVVALERGRVLAIAAEAAPEGAARA
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHH
QLEALMADAAYGKDEAVRTLAEQWPAVGAALRLSAARSISEGYYVGAAMLALALCAAVLV
HHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
MRRPPHAPREIAKGAS
HCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]