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The map label for this gene is rfbF [H]

Identifier: 158425104

GI number: 158425104

Start: 3997253

End: 3998026

Strand: Reverse

Name: rfbF [H]

Synonym: AZC_3480

Alternate gene names: 158425104

Gene position: 3998026-3997253 (Counterclockwise)

Preceding gene: 158425105

Following gene: 158425103

Centisome position: 74.45

GC content: 64.21

Gene sequence:

>774_bases
ATGAAAGTCGTTATTCTGGCCGGCGGGCGGGGCTCGCGTCTCTCCGAGGAGACCAGTGTCCGGCCGAAGCCGCTGGTGGA
GATCGGTGACAAGCCGATCATCTGGCACATCATGAGCATCTATGCTCATCACGGCTTCAACGAGTTCATCGTCTGCGCCG
GCTACAAGGGCTACCAGATCAAGGAATATTTCGCGAACCTCGTGCTCCACCATTCCGACGTGACGGTGGATCTGGGTCGC
AATCTCGTCCACTACCACCAGTCGGACAAGCCGAACTGGCGGGTGAGCATCGTCGACACCGGGCTCGACACCATGACCGG
CGGCCGCCTGAAGCGCGTGCGCCCCTATCTCACCCCCGGCGAACCCTTCCTGATGACCTATGGCGACGGCGTCGCCGACG
TGGACGTTGCGCAGGAAGTGGCCTTCCACAAGGCCCATGGGCTTAAGGCCACCATGTGCGCCGTGGCACCTCCCGGCCGC
TTCGGAAGCGCCAATATCGAGGGCAACAAGGTCTCCTCCTTCGTGGAGAAGCCGACCGCGGGCAACCAGCGCATCAATGG
CGGCTTCTTCGTGCTGGAGCACGACGTTCTCGACCTGATCGACAATGACGACACGGTTTGGGAGGCGGAGCCGCTGGAGT
CGCTGGTGGCGGAGGGGCAGCTCGCGGCCTTCCCGCACGACGGCTTCTGGCGTCCCATGGACACGCTGCGCGAGCGCACC
CAGCTCGACGAGCTCTGGAACTCCGGCCGCGCGCCCTGGAAGGTGTGGGCGTGA

Upstream 100 bases:

>100_bases
CTTTAAGTGGCGCCAACGATCCATGGTCGACTGCCGACTGAACCATGCGCGTGGCCTTCGCGGCCATCATCGGGGATGCG
GGACGTCACGGGGGGATGTG

Downstream 100 bases:

>100_bases
GTGGGTTCTGGGCCGGTCGCCGTGTCCTCGTGACCGGACATACCGGCTTCAAGGGGGCGTGGCTCTGCCTGTGGCTGGAA
CGTCTTGGCGCGCAGGTGAG

Product: glucose-1-phosphate cytidylyltransferase

Products: NA

Alternate protein names: CDP-glucose pyrophosphorylase [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKVVILAGGRGSRLSEETSVRPKPLVEIGDKPIIWHIMSIYAHHGFNEFIVCAGYKGYQIKEYFANLVLHHSDVTVDLGR
NLVHYHQSDKPNWRVSIVDTGLDTMTGGRLKRVRPYLTPGEPFLMTYGDGVADVDVAQEVAFHKAHGLKATMCAVAPPGR
FGSANIEGNKVSSFVEKPTAGNQRINGGFFVLEHDVLDLIDNDDTVWEAEPLESLVAEGQLAAFPHDGFWRPMDTLRERT
QLDELWNSGRAPWKVWA

Sequences:

>Translated_257_residues
MKVVILAGGRGSRLSEETSVRPKPLVEIGDKPIIWHIMSIYAHHGFNEFIVCAGYKGYQIKEYFANLVLHHSDVTVDLGR
NLVHYHQSDKPNWRVSIVDTGLDTMTGGRLKRVRPYLTPGEPFLMTYGDGVADVDVAQEVAFHKAHGLKATMCAVAPPGR
FGSANIEGNKVSSFVEKPTAGNQRINGGFFVLEHDVLDLIDNDDTVWEAEPLESLVAEGQLAAFPHDGFWRPMDTLRERT
QLDELWNSGRAPWKVWA
>Mature_257_residues
MKVVILAGGRGSRLSEETSVRPKPLVEIGDKPIIWHIMSIYAHHGFNEFIVCAGYKGYQIKEYFANLVLHHSDVTVDLGR
NLVHYHQSDKPNWRVSIVDTGLDTMTGGRLKRVRPYLTPGEPFLMTYGDGVADVDVAQEVAFHKAHGLKATMCAVAPPGR
FGSANIEGNKVSSFVEKPTAGNQRINGGFFVLEHDVLDLIDNDDTVWEAEPLESLVAEGQLAAFPHDGFWRPMDTLRERT
QLDELWNSGRAPWKVWA

Specific function: Involved in the biosynthesis of the tyvelose, a 3,6- dideoxyhexose found in the O-antigen of the surface lipopolysaccharides. It catalyzes the transfer of a CMP moiety from CTP to glucose 1-phosphate. This enzyme can utilize either CTP or UTP as the nucle

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate cytidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=236, Percent_Identity=28.3898305084746, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI11761619, Length=236, Percent_Identity=28.3898305084746, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI133931050, Length=247, Percent_Identity=31.1740890688259, Blast_Score=103, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6320148, Length=248, Percent_Identity=33.0645161290323, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21355443, Length=247, Percent_Identity=27.9352226720648, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24644084, Length=247, Percent_Identity=27.9352226720648, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013446
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.33 [H]

Molecular weight: Translated: 28693; Mature: 28693

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVILAGGRGSRLSEETSVRPKPLVEIGDKPIIWHIMSIYAHHGFNEFIVCAGYKGYQI
CEEEEEECCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCHH
KEYFANLVLHHSDVTVDLGRNLVHYHQSDKPNWRVSIVDTGLDTMTGGRLKRVRPYLTPG
HHHHHHHHEECCCEEEECCCCEEECCCCCCCCEEEEEEECCCCCCCCCCHHHCCCCCCCC
EPFLMTYGDGVADVDVAQEVAFHKAHGLKATMCAVAPPGRFGSANIEGNKVSSFVEKPTA
CCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCC
GNQRINGGFFVLEHDVLDLIDNDDTVWEAEPLESLVAEGQLAAFPHDGFWRPMDTLRERT
CCCEECCCEEEEECCHHHHHCCCCCEECCHHHHHHHHCCCEEECCCCCCCCCHHHHHHHH
QLDELWNSGRAPWKVWA
HHHHHHHCCCCCEEECC
>Mature Secondary Structure
MKVVILAGGRGSRLSEETSVRPKPLVEIGDKPIIWHIMSIYAHHGFNEFIVCAGYKGYQI
CEEEEEECCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCHH
KEYFANLVLHHSDVTVDLGRNLVHYHQSDKPNWRVSIVDTGLDTMTGGRLKRVRPYLTPG
HHHHHHHHEECCCEEEECCCCEEECCCCCCCCEEEEEEECCCCCCCCCCHHHCCCCCCCC
EPFLMTYGDGVADVDVAQEVAFHKAHGLKATMCAVAPPGRFGSANIEGNKVSSFVEKPTA
CCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCC
GNQRINGGFFVLEHDVLDLIDNDDTVWEAEPLESLVAEGQLAAFPHDGFWRPMDTLRERT
CCCEECCCEEEEECCHHHHHCCCCCEECCHHHHHHHHCCCEEECCCCCCCCCHHHHHHHH
QLDELWNSGRAPWKVWA
HHHHHHHCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]