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The map label for this gene is clcD [H]

Identifier: 158425058

GI number: 158425058

Start: 3952959

End: 3953810

Strand: Reverse

Name: clcD [H]

Synonym: AZC_3434

Alternate gene names: 158425058

Gene position: 3953810-3952959 (Counterclockwise)

Preceding gene: 158425059

Following gene: 158425057

Centisome position: 73.63

GC content: 69.37

Gene sequence:

>852_bases
ATGCATCGGGGAGGGCAGGGACCGCGACAACCCCGGATCGCCCTGCCGCCCGCAGGGCCGCGTGTCCCGCGCGAGGATGT
CATGTTCCAGCTCACCGCCGCCGACGGCCACGGGCTCTCTGCCTATCGGGCCGAGCCCAAGGACGACGCCAGGGGCGCCG
TCGTGCTCATTCACGACATGTTCGGCATCACCGATGACATGTGCGCCGCCGCCGATGACTTTGCCGCACGGGGCTATCTT
GCCATCGTGCCGGCCCTGTTCGACCGCGCGGCGCCCGGCACTGTGCTCGCCTATGACGAGGAGGGCTTCGCCAGGGGCGT
GACCTTGGTGGAGGAAGTGGGGCGCGAGCCCGCCCTCAACGATATTCAGGCGGCCGTGGACGCGGCGCGGGAGGCCGGTA
AGGTCGCGGTCGTGGGCTATTCCTGGGGCGGGTTTCTCGCCTATGAGGCGGCGAACCAGCTGCGGGGTCTCGCCTGCGCC
ATCGCCTATTATCCTCTTGGTGTCGTCGGGGCCGGTGCAGAGAAGCGGCGCGTGCCGACGCTGATCCATTTCGGCGCCAC
CGATCCGCTGGTGCCGGTGGATCAGGCGCAGCGCTTCCGCGACCAGCGTCCGGATGTGAGCGCCTTCACCTATGAGGGTG
CGACCCATGGCTTTGCCAGCGCCGGCCGGGCCACCTACCACGCCGCCGCCACCTGCGAGGCCCAGGCGCGCACGCTGTTC
TGGATCGGCCAGTTCGTCGAGGGGCAGGCGCCCGTGCAGTTGAAGAATGCCGGCGCCTATGCCCAGGCCAAGACGGAAAA
GAAGAAGGCGAAGGCGCCGGCCGGGGATGATCTTGGCCCGCCGCTCGACTGA

Upstream 100 bases:

>100_bases
CAATCTGGTGTTCGATCCGCCGTGGGACACCAACCGCATGACCGACGACGTGAAGCTGGAGCTGGGTCTTCTCTGATCCG
GAGGCCATCTGGCACCCTCG

Downstream 100 bases:

>100_bases
CCCGGCCGGGGGAGAACGGGATGCGTGAAGGTGAAACGTCCGCGGCGGCCACGGCCTGGGCCGCGCGCCGGCTGCGGACG
CAGCTTGGCCTTGAGGCTCT

Product: carboxymethylenebutenolidase

Products: NA

Alternate protein names: Dienelactone hydrolase; DLH [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MHRGGQGPRQPRIALPPAGPRVPREDVMFQLTAADGHGLSAYRAEPKDDARGAVVLIHDMFGITDDMCAAADDFAARGYL
AIVPALFDRAAPGTVLAYDEEGFARGVTLVEEVGREPALNDIQAAVDAAREAGKVAVVGYSWGGFLAYEAANQLRGLACA
IAYYPLGVVGAGAEKRRVPTLIHFGATDPLVPVDQAQRFRDQRPDVSAFTYEGATHGFASAGRATYHAAATCEAQARTLF
WIGQFVEGQAPVQLKNAGAYAQAKTEKKKAKAPAGDDLGPPLD

Sequences:

>Translated_283_residues
MHRGGQGPRQPRIALPPAGPRVPREDVMFQLTAADGHGLSAYRAEPKDDARGAVVLIHDMFGITDDMCAAADDFAARGYL
AIVPALFDRAAPGTVLAYDEEGFARGVTLVEEVGREPALNDIQAAVDAAREAGKVAVVGYSWGGFLAYEAANQLRGLACA
IAYYPLGVVGAGAEKRRVPTLIHFGATDPLVPVDQAQRFRDQRPDVSAFTYEGATHGFASAGRATYHAAATCEAQARTLF
WIGQFVEGQAPVQLKNAGAYAQAKTEKKKAKAPAGDDLGPPLD
>Mature_283_residues
MHRGGQGPRQPRIALPPAGPRVPREDVMFQLTAADGHGLSAYRAEPKDDARGAVVLIHDMFGITDDMCAAADDFAARGYL
AIVPALFDRAAPGTVLAYDEEGFARGVTLVEEVGREPALNDIQAAVDAAREAGKVAVVGYSWGGFLAYEAANQLRGLACA
IAYYPLGVVGAGAEKRRVPTLIHFGATDPLVPVDQAQRFRDQRPDVSAFTYEGATHGFASAGRATYHAAATCEAQARTLF
WIGQFVEGQAPVQLKNAGAYAQAKTEKKKAKAPAGDDLGPPLD

Specific function: Ring cleavage of cyclic ester dienelactone to produce maleylacetate [H]

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dienelactone hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI48994982, Length=241, Percent_Identity=27.3858921161826, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: =3.1.1.45 [H]

Molecular weight: Translated: 29934; Mature: 29934

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRGGQGPRQPRIALPPAGPRVPREDVMFQLTAADGHGLSAYRAEPKDDARGAVVLIHDM
CCCCCCCCCCCEEECCCCCCCCCCHHEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEHH
FGITDDMCAAADDFAARGYLAIVPALFDRAAPGTVLAYDEEGFARGVTLVEEVGREPALN
HCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHH
DIQAAVDAAREAGKVAVVGYSWGGFLAYEAANQLRGLACAIAYYPLGVVGAGAEKRRVPT
HHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCHHEEECCCCCCCCCE
LIHFGATDPLVPVDQAQRFRDQRPDVSAFTYEGATHGFASAGRATYHAAATCEAQARTLF
EEEECCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCHHHCCCCEEEEEEECCHHHEEEE
WIGQFVEGQAPVQLKNAGAYAQAKTEKKKAKAPAGDDLGPPLD
EEHHHHCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MHRGGQGPRQPRIALPPAGPRVPREDVMFQLTAADGHGLSAYRAEPKDDARGAVVLIHDM
CCCCCCCCCCCEEECCCCCCCCCCHHEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEHH
FGITDDMCAAADDFAARGYLAIVPALFDRAAPGTVLAYDEEGFARGVTLVEEVGREPALN
HCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHH
DIQAAVDAAREAGKVAVVGYSWGGFLAYEAANQLRGLACAIAYYPLGVVGAGAEKRRVPT
HHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCHHEEECCCCCCCCCE
LIHFGATDPLVPVDQAQRFRDQRPDVSAFTYEGATHGFASAGRATYHAAATCEAQARTLF
EEEECCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCHHHCCCCEEEEEEECCHHHEEEE
WIGQFVEGQAPVQLKNAGAYAQAKTEKKKAKAPAGDDLGPPLD
EEHHHHCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3299368 [H]