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The map label for this gene is hsrA [H]

Identifier: 158424995

GI number: 158424995

Start: 3900075

End: 3901526

Strand: Direct

Name: hsrA [H]

Synonym: AZC_3371

Alternate gene names: 158424995

Gene position: 3900075-3901526 (Clockwise)

Preceding gene: 158424993

Following gene: 158425000

Centisome position: 72.63

GC content: 66.94

Gene sequence:

>1452_bases
ATGCTCGCATCTGCCCTGCCCCGCCATATCCTCGTTCCGCTCATCGTGGCGTGCGCATTGTTCATGGAGCAGGTGGATTC
CACCGTCATCTCGACATCTCTGCCCGCCATTGCCGCCTCGCTGCACGATGATCCGGTGGCGCTGAAGCTGGCGCTCACCT
CCTATCTGCTGAGCCTCGCGGTCTTCATTCCCGCGTCGGGCTGGTGTGCGGACCGATTCGGCGCGCGCACCGTGTTCCGC
TCGGCCATCGTCATCTTCACGCTGGGCTCGATCCTCTGCGGGCTCTCCACCAGCCTTCTGGATTTCGTGATCTACCGCAT
CATCCAGGGCATGGGCGGGGCGATGATGGTGCCGGTGGGCCGCCTCGTGATCCTGCGGTCGGTGCCGAAGTCCGAGATCG
TCTCGGCGCTCGCCTGGCTGACCATTCCGGCGCTGCTCGGCCCCGTGATGGGTCCGCCGCTGGGCGGCTTCATCACCACC
TATTTCGACTGGCGGCTCATCTTCTTCATCAACGTGCCCATCGGCATTCTCGGCATCATCCTCGCCACGCGCTTCATCGA
GAACGTGCGCGAGGAGGACGTACCGCCTCTGGACCTGCACGGCCTCGTGCTCTCCGGCATCGGCCTCTCCGGGCTCGTCT
TCGGCCTCGCCGTTCTGGGCCAGCACCTCGTGCCCTATTGGGCGGCGGCGGGCGTCACCGCCATCGGCATCGTGGCCCTC
GGCTTCTATGTCCGCCACGCCCGGCGCACGCCCCATGCGGTGCTGGACCTCTCGCTGCTGCGCATCCCGACCTTCCGCGC
CGGCGTGGTCGGCGGCTCGCTGTTCCGCATCGGCGTGGGCGCCCTGCCCTTCGCTCTGCCGCTGATGCTGCAACTGACCT
TCGGCCTGTCCCCCTTCGCCTCCGGCATGCTCACCTTCGCCTCATCCGCCGGTGCCCTGGTGATGAAGACGACGGCGCGG
CCGATCCTGCGGCGCTTCGGCTTCTGGCGGGTGCTGGTGGTGAACGCGCTGATCTCCTCGGCCTTCATCGGTGCCAATGC
GCTGTTCACGCCGGAAACGCCCCATGCCGCCATCGTCGCGGTGCTGCTGATGGGCGGCTTCTTCCGCTCGCTGGAGTTCA
CCGCCATCAACGCCTTGTCCTATGCGGACGTCTCCCAGCCGGAGATGAGCCGCGCCACCTCCTTCGTCAGCGTGGCGCAG
CAGGTGTCGCTGTCTCTGGGTGTGGCGCTGGTAGGCATGGTGCTGGAGGCAATGCGCAGCGCACGGGGCGAGACGGCACT
GCAACTGGCCGACTTCACCCCCGCCTTCCTCATCGTCGCAGGAGTGAGCGCGCTTGCCGTCTTCAGCTTCATGAACCTGC
CGAAGGATGCGGGCGCAGAACTCTCCGGCCACAAGGAAAAGCCGAGCACGCTGCCCGATCCCCGCGGCGAGGTGAACACC
GCGGGCTCGTAA

Upstream 100 bases:

>100_bases
TGAACGTCCGAACCTTGCGCCACGGGCTGGAGGAACGGCGGCCAGGAGGCTAGATGAGGGACGCGCCTTCCCCGCCGCAC
CGTGCCCTTCCGGAGCGCAC

Downstream 100 bases:

>100_bases
GGTTCAGGCCTCGTCCGTCTCGTCTTCCGTCCGGTCGGCGCTGCCGCGAAAACCGGTCGCGACGAGATAGAGCTCGGCCG
AATCCGCACGGCTCGCCGCC

Product: multidrug efflux protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MLASALPRHILVPLIVACALFMEQVDSTVISTSLPAIAASLHDDPVALKLALTSYLLSLAVFIPASGWCADRFGARTVFR
SAIVIFTLGSILCGLSTSLLDFVIYRIIQGMGGAMMVPVGRLVILRSVPKSEIVSALAWLTIPALLGPVMGPPLGGFITT
YFDWRLIFFINVPIGILGIILATRFIENVREEDVPPLDLHGLVLSGIGLSGLVFGLAVLGQHLVPYWAAAGVTAIGIVAL
GFYVRHARRTPHAVLDLSLLRIPTFRAGVVGGSLFRIGVGALPFALPLMLQLTFGLSPFASGMLTFASSAGALVMKTTAR
PILRRFGFWRVLVVNALISSAFIGANALFTPETPHAAIVAVLLMGGFFRSLEFTAINALSYADVSQPEMSRATSFVSVAQ
QVSLSLGVALVGMVLEAMRSARGETALQLADFTPAFLIVAGVSALAVFSFMNLPKDAGAELSGHKEKPSTLPDPRGEVNT
AGS

Sequences:

>Translated_483_residues
MLASALPRHILVPLIVACALFMEQVDSTVISTSLPAIAASLHDDPVALKLALTSYLLSLAVFIPASGWCADRFGARTVFR
SAIVIFTLGSILCGLSTSLLDFVIYRIIQGMGGAMMVPVGRLVILRSVPKSEIVSALAWLTIPALLGPVMGPPLGGFITT
YFDWRLIFFINVPIGILGIILATRFIENVREEDVPPLDLHGLVLSGIGLSGLVFGLAVLGQHLVPYWAAAGVTAIGIVAL
GFYVRHARRTPHAVLDLSLLRIPTFRAGVVGGSLFRIGVGALPFALPLMLQLTFGLSPFASGMLTFASSAGALVMKTTAR
PILRRFGFWRVLVVNALISSAFIGANALFTPETPHAAIVAVLLMGGFFRSLEFTAINALSYADVSQPEMSRATSFVSVAQ
QVSLSLGVALVGMVLEAMRSARGETALQLADFTPAFLIVAGVSALAVFSFMNLPKDAGAELSGHKEKPSTLPDPRGEVNT
AGS
>Mature_483_residues
MLASALPRHILVPLIVACALFMEQVDSTVISTSLPAIAASLHDDPVALKLALTSYLLSLAVFIPASGWCADRFGARTVFR
SAIVIFTLGSILCGLSTSLLDFVIYRIIQGMGGAMMVPVGRLVILRSVPKSEIVSALAWLTIPALLGPVMGPPLGGFITT
YFDWRLIFFINVPIGILGIILATRFIENVREEDVPPLDLHGLVLSGIGLSGLVFGLAVLGQHLVPYWAAAGVTAIGIVAL
GFYVRHARRTPHAVLDLSLLRIPTFRAGVVGGSLFRIGVGALPFALPLMLQLTFGLSPFASGMLTFASSAGALVMKTTAR
PILRRFGFWRVLVVNALISSAFIGANALFTPETPHAAIVAVLLMGGFFRSLEFTAINALSYADVSQPEMSRATSFVSVAQ
QVSLSLGVALVGMVLEAMRSARGETALQLADFTPAFLIVAGVSALAVFSFMNLPKDAGAELSGHKEKPSTLPDPRGEVNT
AGS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=464, Percent_Identity=37.7155172413793, Blast_Score=317, Evalue=2e-87,
Organism=Escherichia coli, GI1788392, Length=467, Percent_Identity=35.5460385438972, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI1788710, Length=439, Percent_Identity=27.1070615034169, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1789042, Length=427, Percent_Identity=25.9953161592506, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI87081983, Length=321, Percent_Identity=26.1682242990654, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6325455, Length=233, Percent_Identity=25.7510729613734, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323735, Length=202, Percent_Identity=28.7128712871287, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51009; Mature: 51009

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLASALPRHILVPLIVACALFMEQVDSTVISTSLPAIAASLHDDPVALKLALTSYLLSLA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VFIPASGWCADRFGARTVFRSAIVIFTLGSILCGLSTSLLDFVIYRIIQGMGGAMMVPVG
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHH
RLVILRSVPKSEIVSALAWLTIPALLGPVMGPPLGGFITTYFDWRLIFFINVPIGILGII
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEEEEEEECHHHHHHHH
LATRFIENVREEDVPPLDLHGLVLSGIGLSGLVFGLAVLGQHLVPYWAAAGVTAIGIVAL
HHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFYVRHARRTPHAVLDLSLLRIPTFRAGVVGGSLFRIGVGALPFALPLMLQLTFGLSPFA
HHHHHHHCCCCHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHH
SGMLTFASSAGALVMKTTARPILRRFGFWRVLVVNALISSAFIGANALFTPETPHAAIVA
HHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHH
VLLMGGFFRSLEFTAINALSYADVSQPEMSRATSFVSVAQQVSLSLGVALVGMVLEAMRS
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARGETALQLADFTPAFLIVAGVSALAVFSFMNLPKDAGAELSGHKEKPSTLPDPRGEVNT
CCCCCEEEHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AGS
CCC
>Mature Secondary Structure
MLASALPRHILVPLIVACALFMEQVDSTVISTSLPAIAASLHDDPVALKLALTSYLLSLA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VFIPASGWCADRFGARTVFRSAIVIFTLGSILCGLSTSLLDFVIYRIIQGMGGAMMVPVG
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHH
RLVILRSVPKSEIVSALAWLTIPALLGPVMGPPLGGFITTYFDWRLIFFINVPIGILGII
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEEEEEEECHHHHHHHH
LATRFIENVREEDVPPLDLHGLVLSGIGLSGLVFGLAVLGQHLVPYWAAAGVTAIGIVAL
HHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFYVRHARRTPHAVLDLSLLRIPTFRAGVVGGSLFRIGVGALPFALPLMLQLTFGLSPFA
HHHHHHHCCCCHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHH
SGMLTFASSAGALVMKTTARPILRRFGFWRVLVVNALISSAFIGANALFTPETPHAAIVA
HHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHH
VLLMGGFFRSLEFTAINALSYADVSQPEMSRATSFVSVAQQVSLSLGVALVGMVLEAMRS
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARGETALQLADFTPAFLIVAGVSALAVFSFMNLPKDAGAELSGHKEKPSTLPDPRGEVNT
CCCCCEEEHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AGS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]