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The map label for this gene is potH [H]

Identifier: 158424969

GI number: 158424969

Start: 3873083

End: 3873985

Strand: Direct

Name: potH [H]

Synonym: AZC_3345

Alternate gene names: 158424969

Gene position: 3873083-3873985 (Clockwise)

Preceding gene: 158424968

Following gene: 158424970

Centisome position: 72.13

GC content: 66.45

Gene sequence:

>903_bases
ATGAGCCGCCCCGTGTCCCTCGACGCGCCCCGCATCTCCCTGTCGCAGCGCCTCGCCGCCCGAGGCATCGACGGCCTGAC
GCTGCTGGTTCTGCCGGCCGCGCTCTTCATCCTTGGCCTGTTCGTCTATCCCTTCCTCTATGGGCTCTGGCTGTCGTTCC
AGCCCAAGGTGGGCGGCACGCTGGCCAATTATGTGAAGTTCTTCTCCGACCCGTTCCTCTACGGGACCATCGCCACCACC
TTGTGGCTGGCGCTGCCGGTGACGATCCTCAACATCGTGCTCGCGGTGCCGATCGCGCTGCGCGTACGCCACCTGCGTCG
CCAGAGGCTGCTGACCACCATCCTCGTGATCCCGATCACGCTGGGCACGGTGCTCGTGGCGGAAGGCCTGCTGAACTATC
TGGGGCCGCAGGGCTGGTTCAACCGCACGCTCATCACCCTCGGCCTCATCTCCAAGCCGCTGGCGCTGCTCCACAATTAC
TGGGGCGTGTTCATCTCGCTGCTGCTGACGGGCTTCCCCTTCACCTTCCTGCTCACCCTCTCCTATCTATCGGGCCTCGA
CCCGGCGCTGGAGCAGGCAGCGGCGACGTTGGGGGCGGGATCGTGGCAGCGGTTCCGGCACATCATCCTGCCGCTGCTGC
TGGCGGGCCTTGCCATCACCTTCTGCCTGTCCTTCGTGCAGGCCTTCTCGGTGTTTCCCTCGGCGGTGCTGCTGGGTGCC
CCGGCCGGGCCCACCCGCGTCATCTCGATCGCGGCCTATCAGGCGGCCTTCGAGGACTATGATTATTCCATGGCCTCGGC
CATCGCCATGATCATGGCCGTGGTGCAGCTGGCCATCGTCGCGGCGCTTCTGTTCGCCCGCGGCCTCATCTATCGCGGCG
CCACCTCCGGCGGAAAGGGCTGA

Upstream 100 bases:

>100_bases
CGGATCTCGCCTTCCTTTCCCACAGTCCGCTGGTGGCCGGCGATCAGGTTCAGGTCTCCGCCGATCCCGCCCGCGTCCTC
GTCTTCCCGGATCGCACCCA

Downstream 100 bases:

>100_bases
GCCATGATCCGGGATACGACACTCGGCGCGAAGCTCTGGATCACCGCCGTCTGGGCCGTGATCGGCTTCTTCATCCTGAA
CCTCCTCGCCATGATCGCGG

Product: ABC transporter membrane spanning protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MSRPVSLDAPRISLSQRLAARGIDGLTLLVLPAALFILGLFVYPFLYGLWLSFQPKVGGTLANYVKFFSDPFLYGTIATT
LWLALPVTILNIVLAVPIALRVRHLRRQRLLTTILVIPITLGTVLVAEGLLNYLGPQGWFNRTLITLGLISKPLALLHNY
WGVFISLLLTGFPFTFLLTLSYLSGLDPALEQAAATLGAGSWQRFRHIILPLLLAGLAITFCLSFVQAFSVFPSAVLLGA
PAGPTRVISIAAYQAAFEDYDYSMASAIAMIMAVVQLAIVAALLFARGLIYRGATSGGKG

Sequences:

>Translated_300_residues
MSRPVSLDAPRISLSQRLAARGIDGLTLLVLPAALFILGLFVYPFLYGLWLSFQPKVGGTLANYVKFFSDPFLYGTIATT
LWLALPVTILNIVLAVPIALRVRHLRRQRLLTTILVIPITLGTVLVAEGLLNYLGPQGWFNRTLITLGLISKPLALLHNY
WGVFISLLLTGFPFTFLLTLSYLSGLDPALEQAAATLGAGSWQRFRHIILPLLLAGLAITFCLSFVQAFSVFPSAVLLGA
PAGPTRVISIAAYQAAFEDYDYSMASAIAMIMAVVQLAIVAALLFARGLIYRGATSGGKG
>Mature_299_residues
SRPVSLDAPRISLSQRLAARGIDGLTLLVLPAALFILGLFVYPFLYGLWLSFQPKVGGTLANYVKFFSDPFLYGTIATTL
WLALPVTILNIVLAVPIALRVRHLRRQRLLTTILVIPITLGTVLVAEGLLNYLGPQGWFNRTLITLGLISKPLALLHNYW
GVFISLLLTGFPFTFLLTLSYLSGLDPALEQAAATLGAGSWQRFRHIILPLLLAGLAITFCLSFVQAFSVFPSAVLLGAP
AGPTRVISIAAYQAAFEDYDYSMASAIAMIMAVVQLAIVAALLFARGLIYRGATSGGKG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1175

COG function: function code G; ABC-type sugar transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=253, Percent_Identity=26.8774703557312, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787570, Length=248, Percent_Identity=26.6129032258064, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787369, Length=217, Percent_Identity=27.6497695852535, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788764, Length=214, Percent_Identity=26.1682242990654, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786254, Length=228, Percent_Identity=27.6315789473684, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32397; Mature: 32266

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPVSLDAPRISLSQRLAARGIDGLTLLVLPAALFILGLFVYPFLYGLWLSFQPKVGGT
CCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
LANYVKFFSDPFLYGTIATTLWLALPVTILNIVLAVPIALRVRHLRRQRLLTTILVIPIT
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGTVLVAEGLLNYLGPQGWFNRTLITLGLISKPLALLHNYWGVFISLLLTGFPFTFLLTL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
SYLSGLDPALEQAAATLGAGSWQRFRHIILPLLLAGLAITFCLSFVQAFSVFPSAVLLGA
HHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PAGPTRVISIAAYQAAFEDYDYSMASAIAMIMAVVQLAIVAALLFARGLIYRGATSGGKG
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SRPVSLDAPRISLSQRLAARGIDGLTLLVLPAALFILGLFVYPFLYGLWLSFQPKVGGT
CCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH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PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]