Click here to switch to the map view.

The map label for this gene is yiaO [H]

Identifier: 158424923

GI number: 158424923

Start: 3811466

End: 3812500

Strand: Direct

Name: yiaO [H]

Synonym: AZC_3299

Alternate gene names: 158424923

Gene position: 3811466-3812500 (Clockwise)

Preceding gene: 158424922

Following gene: 158424924

Centisome position: 70.98

GC content: 64.15

Gene sequence:

>1035_bases
ATGACCTTTTCCCGCGTCATTTCCCGCCGCCATGTGCTCGCCGGTGCCCTCGCCGCCCCCGCTGTCCTCAGCTTCGCGCG
CCTCGGCCATGCGGCGACCACGCTCAAGATCTCGCATCAGTTCCCGAGCTCCAGCGGCGACGAGGGCGATTTCCGCGACC
GCCTCGTCCGCCGCTTCGCCGCCGAGGTGGAGAAGCGCTCCAATGGCGAATTGAAGTTCGAGATCTATCCCGGTTCGTCG
CTGATGAAGACGAACTCCCAATTCTCGGCCCTGCGCAAGGGCGCACTCGATTTCTCGCTCTATCCCATCTCCTACGCCGG
CGGCGAGGCCCCCGAGTGCAACATCGGCCTCATGCCGTGCCTTGTCTCCTCCTACGCGCAGGGTCTCGCCTGGAAGACCA
AGCCGGTGGGCGAGGAGTTCAACGCCTATCTGGAAGGCAAGGGCGTCAAGATCATCACCTGGGTCTGGCAGGCGGGCGGT
GCCGCCAGCCGCGAGAAGCCGCTGGTCTCGCCCGAGGATGCCAAGGGCATGAAGATCCGCGGCGGCTCGCGCGAGATGGA
CCTGATGCTTCAGGCGGCGGGTGCTGCTGTGCTCAACCTGCCGTCCAACGAACTCTATGTGGCGATGCAGACCGGCGCCT
GCGATGCCGGCATCACCTCCTCCACCAGCCTCATCTCCTTCCGGCTGGAGGAACTGTCGAAGAACCTCGCCGCCGGCAAG
GGCAAGTCCTACTGGTTCATGCTTGAGCCGCTGCTCATGTCCAAGGCCATCTTCGAGAAGTTGCCCAAGGAGCAGCAGGA
TCTGATCCTCAAGGTCGGGGCGGAGATGGAGCCCTTTGGCCTCAAGGGCGCTCAGGAAGACGACCACAAGGTGGTGGAGG
TGTTCCAGAAGGCCGGCGCCAAGATCCATGATCTCGACGACGCGGTCCTGGAGAAGTGGAAGGTGATCGCCCGCGACACT
GCCTGGAAGGACTATGCCGCCAAGACGCCCCTCTCCGCGAAGCTGCTGAAGCTCGCCGAAGAGGTGCAGGCATGA

Upstream 100 bases:

>100_bases
GTCTCCGGCTCAAGATCCCGAACAATCGGGACGGCCGGGAGGAAACGCCAACCGCCGAGGCACCGCGTCAGCCACGCGGC
ATGCCCCAGGGAGGACTGCC

Downstream 100 bases:

>100_bases
GCGCACACGGGTTCGAGCTTCCAAGCGCCGGCGCCTCTCCGGAGGCGCGGGCACTCAGCGCGGCGCAGGCGGCACACCCT
CTCCTGGGTGTGCTGCAAGG

Product: twin-arginine translocation pathway signal precursor

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MTFSRVISRRHVLAGALAAPAVLSFARLGHAATTLKISHQFPSSSGDEGDFRDRLVRRFAAEVEKRSNGELKFEIYPGSS
LMKTNSQFSALRKGALDFSLYPISYAGGEAPECNIGLMPCLVSSYAQGLAWKTKPVGEEFNAYLEGKGVKIITWVWQAGG
AASREKPLVSPEDAKGMKIRGGSREMDLMLQAAGAAVLNLPSNELYVAMQTGACDAGITSSTSLISFRLEELSKNLAAGK
GKSYWFMLEPLLMSKAIFEKLPKEQQDLILKVGAEMEPFGLKGAQEDDHKVVEVFQKAGAKIHDLDDAVLEKWKVIARDT
AWKDYAAKTPLSAKLLKLAEEVQA

Sequences:

>Translated_344_residues
MTFSRVISRRHVLAGALAAPAVLSFARLGHAATTLKISHQFPSSSGDEGDFRDRLVRRFAAEVEKRSNGELKFEIYPGSS
LMKTNSQFSALRKGALDFSLYPISYAGGEAPECNIGLMPCLVSSYAQGLAWKTKPVGEEFNAYLEGKGVKIITWVWQAGG
AASREKPLVSPEDAKGMKIRGGSREMDLMLQAAGAAVLNLPSNELYVAMQTGACDAGITSSTSLISFRLEELSKNLAAGK
GKSYWFMLEPLLMSKAIFEKLPKEQQDLILKVGAEMEPFGLKGAQEDDHKVVEVFQKAGAKIHDLDDAVLEKWKVIARDT
AWKDYAAKTPLSAKLLKLAEEVQA
>Mature_343_residues
TFSRVISRRHVLAGALAAPAVLSFARLGHAATTLKISHQFPSSSGDEGDFRDRLVRRFAAEVEKRSNGELKFEIYPGSSL
MKTNSQFSALRKGALDFSLYPISYAGGEAPECNIGLMPCLVSSYAQGLAWKTKPVGEEFNAYLEGKGVKIITWVWQAGGA
ASREKPLVSPEDAKGMKIRGGSREMDLMLQAAGAAVLNLPSNELYVAMQTGACDAGITSSTSLISFRLEELSKNLAAGKG
KSYWFMLEPLLMSKAIFEKLPKEQQDLILKVGAEMEPFGLKGAQEDDHKVVEVFQKAGAKIHDLDDAVLEKWKVIARDTA
WKDYAAKTPLSAKLLKLAEEVQA

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=292, Percent_Identity=28.4246575342466, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 37492; Mature: 37360

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSRVISRRHVLAGALAAPAVLSFARLGHAATTLKISHQFPSSSGDEGDFRDRLVRRFA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHH
AEVEKRSNGELKFEIYPGSSLMKTNSQFSALRKGALDFSLYPISYAGGEAPECNIGLMPC
HHHHHCCCCCEEEEEECCCHHHHCCHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHH
LVSSYAQGLAWKTKPVGEEFNAYLEGKGVKIITWVWQAGGAASREKPLVSPEDAKGMKIR
HHHHHHHCCCCCCCCCCHHHHHEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEE
GGSREMDLMLQAAGAAVLNLPSNELYVAMQTGACDAGITSSTSLISFRLEELSKNLAAGK
CCCCHHHHHHHHCCCEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
GKSYWFMLEPLLMSKAIFEKLPKEQQDLILKVGAEMEPFGLKGAQEDDHKVVEVFQKAGA
CCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
KIHDLDDAVLEKWKVIARDTAWKDYAAKTPLSAKLLKLAEEVQA
EEECCHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TFSRVISRRHVLAGALAAPAVLSFARLGHAATTLKISHQFPSSSGDEGDFRDRLVRRFA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHH
AEVEKRSNGELKFEIYPGSSLMKTNSQFSALRKGALDFSLYPISYAGGEAPECNIGLMPC
HHHHHCCCCCEEEEEECCCHHHHCCHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHH
LVSSYAQGLAWKTKPVGEEFNAYLEGKGVKIITWVWQAGGAASREKPLVSPEDAKGMKIR
HHHHHHHCCCCCCCCCCHHHHHEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEE
GGSREMDLMLQAAGAAVLNLPSNELYVAMQTGACDAGITSSTSLISFRLEELSKNLAAGK
CCCCHHHHHHHHCCCEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
GKSYWFMLEPLLMSKAIFEKLPKEQQDLILKVGAEMEPFGLKGAQEDDHKVVEVFQKAGA
CCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
KIHDLDDAVLEKWKVIARDTAWKDYAAKTPLSAKLLKLAEEVQA
EEECCHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]