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The map label for this gene is alkJ [H]

Identifier: 158424892

GI number: 158424892

Start: 3773030

End: 3774661

Strand: Reverse

Name: alkJ [H]

Synonym: AZC_3268

Alternate gene names: 158424892

Gene position: 3774661-3773030 (Counterclockwise)

Preceding gene: 158424893

Following gene: 158424891

Centisome position: 70.29

GC content: 66.24

Gene sequence:

>1632_bases
ATGCCTCAGGCCACCGGCTCGGGCGAAACCTATGATTATGTGATCGTCGGCGCAGGATCCGCCGGCTGCGTTCTGGCCAA
CCGCCTCTCGGCCGATCCGAATGTGCGGGTCGCGGTGGTTGAGGCCGGAGGGCGCGACAACTGGATCTGGTTCCACATTC
CCGTGGGCTATCTCTTCGCCATCGGCAATCCGCGCGCCGACTGGTGCTTCAAGACCGAGCCGGAGCCGGGGCTCAATGGC
CGCGCGCTCTCCTATCCCCGCGGCAAGACCATTGGTGGCTCCTCGGCCATCAACGCCATGATCTACATGCGCGGGCAGGC
GGCGGATTACGACCACTGGCGCCAGCTCGGCCTTGCCGGCTGGGGCTGGGAGGATGTGGAGCCGCTCTTCAAGGCGCATG
AGGACCATTTCCTCGGCCCCAACCCCCATCACGGCAAGGGCGGGGAATGGCATGTGGAGTTCCCGCGCATCACCTGGCCG
CTGCTCGACGTGGTGCGGCAGGCCGCGGACGAGGATGGCATCCGCGCCATCGACGACTTCAATACGGGCGACAATGAAGG
CGCCAGCTACTTCCACGTGAACCAGAAGCGGGGCCTGCGCTGGAGCGCCGCGCGCGGCTTCCTGAAACCGGTGCTCAACC
GGCCTAACCTCGACCTCATTACCACGGGACACGTGGAGAAGGTGCTGGTGCACGAGAAGCGCGCCACCGGCATTCGCGTG
CGGGGCAAGGACGGCCTGCGCACGCTCAACGCCCGCCGGGAGGTGATCCTGGCCGCAGGGGCGCTTGGCTCGCCGCAATT
GCTGATGCTGTCGGGCATCGGTCCGGCCGAGCATCTGGCGGCGAAGGGCATCGATGTTGTGCTCGACAGGCCGGGTGTCG
GGTCGAACCTGCAGGACCACCTGCAACTGCGTCTTATCTACAAGGTGTCCGGTATCGGCACGCTCAACCAGCGTTATCAT
TCGCTGTTCGGGCGCATGGGCATGGGCGTGGAATATGCGCTCTTCCGGCGCGGGCCGCTCACCATGGCGCCCTCGCAGAT
GGGCATCTTCACGAAATCCGGCCCCGAGCAGGAGCGCGCCAATATCGAGTTCCACGTGCAGCCGCTGTCGCTCGACAAGT
TCGGCGAGCCGCTGCACCGCTATCCGGCCTTCACCATGAGCGTGTGCAACCTGCGTCCCACCTCGCGCGGCACGATCCGC
CTCAAGGGGCCGAACCCGTTGGCGCCGCCGGAAATCCGGCCGAACTATCTGGATACGGAAGAGGATCGCAAGGTGGCCGC
GGATTCCATCCGTGTGGCGCGCCGGATCGTCGGGCAGCCGGCGCTCAGGCCCTATCACCCGGAGGAGATGCTGCCGGGGC
CTCAGGTCTCCGATACCGATGAGGCGGCGCTCGCCAAGGCGGCGGGCGACGTGGGCACCACCATCTTCCATCCCGTCGGC
ACGGCGAAGATGGGCCTGCCCTCGGACCCGCTTGCGGTGGTGGACGAGCGACTGCGGGTGTTCGGCATCGCGGGCCTGCG
CGTGGTGGATGCGTCCATCATGCCTGCCATTGTCTCAGGAAACACCAATTCCCCCACCATCATGATCGCCGAAAAGGGCG
CGCGCATGATCCTGGAGGATGCCAAAGCATGA

Upstream 100 bases:

>100_bases
CGCCCGCGCCTTTCGTCGCCCTTGCCCAAGCCTCAGCCTGACGCCACAGTCGGCGCCAAGCCGGCGCAAGTCCGGATGAG
TCCCATGGGAGGAATGCGGG

Downstream 100 bases:

>100_bases
TGAGCCAACGCTTCAAGCGCACGTGGGGTGCTGCTGCCGCCGGACTTGCTCTGGCGTTTCTTGCCGCCCCGGCTGAAGCC
TCGAGCCCCGATGCCTGGGC

Product: glucose-methanol-choline oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MPQATGSGETYDYVIVGAGSAGCVLANRLSADPNVRVAVVEAGGRDNWIWFHIPVGYLFAIGNPRADWCFKTEPEPGLNG
RALSYPRGKTIGGSSAINAMIYMRGQAADYDHWRQLGLAGWGWEDVEPLFKAHEDHFLGPNPHHGKGGEWHVEFPRITWP
LLDVVRQAADEDGIRAIDDFNTGDNEGASYFHVNQKRGLRWSAARGFLKPVLNRPNLDLITTGHVEKVLVHEKRATGIRV
RGKDGLRTLNARREVILAAGALGSPQLLMLSGIGPAEHLAAKGIDVVLDRPGVGSNLQDHLQLRLIYKVSGIGTLNQRYH
SLFGRMGMGVEYALFRRGPLTMAPSQMGIFTKSGPEQERANIEFHVQPLSLDKFGEPLHRYPAFTMSVCNLRPTSRGTIR
LKGPNPLAPPEIRPNYLDTEEDRKVAADSIRVARRIVGQPALRPYHPEEMLPGPQVSDTDEAALAKAAGDVGTTIFHPVG
TAKMGLPSDPLAVVDERLRVFGIAGLRVVDASIMPAIVSGNTNSPTIMIAEKGARMILEDAKA

Sequences:

>Translated_543_residues
MPQATGSGETYDYVIVGAGSAGCVLANRLSADPNVRVAVVEAGGRDNWIWFHIPVGYLFAIGNPRADWCFKTEPEPGLNG
RALSYPRGKTIGGSSAINAMIYMRGQAADYDHWRQLGLAGWGWEDVEPLFKAHEDHFLGPNPHHGKGGEWHVEFPRITWP
LLDVVRQAADEDGIRAIDDFNTGDNEGASYFHVNQKRGLRWSAARGFLKPVLNRPNLDLITTGHVEKVLVHEKRATGIRV
RGKDGLRTLNARREVILAAGALGSPQLLMLSGIGPAEHLAAKGIDVVLDRPGVGSNLQDHLQLRLIYKVSGIGTLNQRYH
SLFGRMGMGVEYALFRRGPLTMAPSQMGIFTKSGPEQERANIEFHVQPLSLDKFGEPLHRYPAFTMSVCNLRPTSRGTIR
LKGPNPLAPPEIRPNYLDTEEDRKVAADSIRVARRIVGQPALRPYHPEEMLPGPQVSDTDEAALAKAAGDVGTTIFHPVG
TAKMGLPSDPLAVVDERLRVFGIAGLRVVDASIMPAIVSGNTNSPTIMIAEKGARMILEDAKA
>Mature_542_residues
PQATGSGETYDYVIVGAGSAGCVLANRLSADPNVRVAVVEAGGRDNWIWFHIPVGYLFAIGNPRADWCFKTEPEPGLNGR
ALSYPRGKTIGGSSAINAMIYMRGQAADYDHWRQLGLAGWGWEDVEPLFKAHEDHFLGPNPHHGKGGEWHVEFPRITWPL
LDVVRQAADEDGIRAIDDFNTGDNEGASYFHVNQKRGLRWSAARGFLKPVLNRPNLDLITTGHVEKVLVHEKRATGIRVR
GKDGLRTLNARREVILAAGALGSPQLLMLSGIGPAEHLAAKGIDVVLDRPGVGSNLQDHLQLRLIYKVSGIGTLNQRYHS
LFGRMGMGVEYALFRRGPLTMAPSQMGIFTKSGPEQERANIEFHVQPLSLDKFGEPLHRYPAFTMSVCNLRPTSRGTIRL
KGPNPLAPPEIRPNYLDTEEDRKVAADSIRVARRIVGQPALRPYHPEEMLPGPQVSDTDEAALAKAAGDVGTTIFHPVGT
AKMGLPSDPLAVVDERLRVFGIAGLRVVDASIMPAIVSGNTNSPTIMIAEKGARMILEDAKA

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=540, Percent_Identity=40, Blast_Score=368, Evalue=1e-102,
Organism=Escherichia coli, GI1786503, Length=536, Percent_Identity=39.365671641791, Blast_Score=326, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI17532301, Length=547, Percent_Identity=35.8318098720293, Blast_Score=324, Evalue=6e-89,
Organism=Drosophila melanogaster, GI24642042, Length=568, Percent_Identity=36.2676056338028, Blast_Score=321, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24642055, Length=577, Percent_Identity=37.0883882149047, Blast_Score=318, Evalue=5e-87,
Organism=Drosophila melanogaster, GI24642048, Length=572, Percent_Identity=36.8881118881119, Blast_Score=315, Evalue=8e-86,
Organism=Drosophila melanogaster, GI45551458, Length=572, Percent_Identity=36.013986013986, Blast_Score=299, Evalue=3e-81,
Organism=Drosophila melanogaster, GI45549471, Length=572, Percent_Identity=36.013986013986, Blast_Score=299, Evalue=4e-81,
Organism=Drosophila melanogaster, GI24642059, Length=570, Percent_Identity=36.8421052631579, Blast_Score=299, Evalue=4e-81,
Organism=Drosophila melanogaster, GI18859995, Length=566, Percent_Identity=37.8091872791519, Blast_Score=287, Evalue=2e-77,
Organism=Drosophila melanogaster, GI17137792, Length=558, Percent_Identity=34.2293906810036, Blast_Score=278, Evalue=6e-75,
Organism=Drosophila melanogaster, GI24642039, Length=564, Percent_Identity=35.1063829787234, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24650267, Length=580, Percent_Identity=32.9310344827586, Blast_Score=271, Evalue=9e-73,
Organism=Drosophila melanogaster, GI24642035, Length=575, Percent_Identity=34.2608695652174, Blast_Score=262, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24642051, Length=574, Percent_Identity=32.7526132404181, Blast_Score=258, Evalue=1e-68,
Organism=Drosophila melanogaster, GI18859993, Length=584, Percent_Identity=32.0205479452055, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24642037, Length=565, Percent_Identity=32.0353982300885, Blast_Score=234, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24645930, Length=582, Percent_Identity=31.0996563573883, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24642057, Length=587, Percent_Identity=30.1533219761499, Blast_Score=162, Evalue=7e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 59220; Mature: 59089

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQATGSGETYDYVIVGAGSAGCVLANRLSADPNVRVAVVEAGGRDNWIWFHIPVGYLFA
CCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCEEEEEEECCCCCCEEEEEECCEEEEE
IGNPRADWCFKTEPEPGLNGRALSYPRGKTIGGSSAINAMIYMRGQAADYDHWRQLGLAG
ECCCCCCCEEECCCCCCCCCCEECCCCCCCCCCCHHEEEEEEEECCCCCHHHHHHHCCCC
WGWEDVEPLFKAHEDHFLGPNPHHGKGGEWHVEFPRITWPLLDVVRQAADEDGIRAIDDF
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCEEEECC
NTGDNEGASYFHVNQKRGLRWSAARGFLKPVLNRPNLDLITTGHVEKVLVHEKRATGIRV
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCCEEE
RGKDGLRTLNARREVILAAGALGSPQLLMLSGIGPAEHLAAKGIDVVLDRPGVGSNLQDH
ECCCCCHHHCCCCEEEEEECCCCCCCEEEEECCCHHHHHHHCCCEEEEECCCCCCCHHHC
LQLRLIYKVSGIGTLNQRYHSLFGRMGMGVEYALFRRGPLTMAPSQMGIFTKSGPEQERA
EEEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEECCCCEECCCCCCEEECCCCCHHHC
NIEFHVQPLSLDKFGEPLHRYPAFTMSVCNLRPTSRGTIRLKGPNPLAPPEIRPNYLDTE
CEEEEEEECCHHHHCCHHHHCCCCEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCC
EDRKVAADSIRVARRIVGQPALRPYHPEEMLPGPQVSDTDEAALAKAAGDVGTTIFHPVG
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHCCCCCCEEECCCC
TAKMGLPSDPLAVVDERLRVFGIAGLRVVDASIMPAIVSGNTNSPTIMIAEKGARMILED
CCCCCCCCCCHHHHHHHHHEEEECCEEEECCCCCCEEEECCCCCCEEEEECCCCEEHHHC
AKA
CCC
>Mature Secondary Structure 
PQATGSGETYDYVIVGAGSAGCVLANRLSADPNVRVAVVEAGGRDNWIWFHIPVGYLFA
CCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCEEEEEEECCCCCCEEEEEECCEEEEE
IGNPRADWCFKTEPEPGLNGRALSYPRGKTIGGSSAINAMIYMRGQAADYDHWRQLGLAG
ECCCCCCCEEECCCCCCCCCCEECCCCCCCCCCCHHEEEEEEEECCCCCHHHHHHHCCCC
WGWEDVEPLFKAHEDHFLGPNPHHGKGGEWHVEFPRITWPLLDVVRQAADEDGIRAIDDF
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCEEEECC
NTGDNEGASYFHVNQKRGLRWSAARGFLKPVLNRPNLDLITTGHVEKVLVHEKRATGIRV
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCCEEE
RGKDGLRTLNARREVILAAGALGSPQLLMLSGIGPAEHLAAKGIDVVLDRPGVGSNLQDH
ECCCCCHHHCCCCEEEEEECCCCCCCEEEEECCCHHHHHHHCCCEEEEECCCCCCCHHHC
LQLRLIYKVSGIGTLNQRYHSLFGRMGMGVEYALFRRGPLTMAPSQMGIFTKSGPEQERA
EEEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEECCCCEECCCCCCEEECCCCCHHHC
NIEFHVQPLSLDKFGEPLHRYPAFTMSVCNLRPTSRGTIRLKGPNPLAPPEIRPNYLDTE
CEEEEEEECCHHHHCCHHHHCCCCEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCC
EDRKVAADSIRVARRIVGQPALRPYHPEEMLPGPQVSDTDEAALAKAAGDVGTTIFHPVG
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHCCCCCCEEECCCC
TAKMGLPSDPLAVVDERLRVFGIAGLRVVDASIMPAIVSGNTNSPTIMIAEKGARMILED
CCCCCCCCCCHHHHHHHHHEEEECCEEEECCCCCCEEEECCCCCCEEEEECCCCEEHHHC
AKA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]