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The map label for this gene is ligE [H]

Identifier: 158424890

GI number: 158424890

Start: 3771882

End: 3772703

Strand: Direct

Name: ligE [H]

Synonym: AZC_3266

Alternate gene names: 158424890

Gene position: 3771882-3772703 (Clockwise)

Preceding gene: 158424889

Following gene: 158424895

Centisome position: 70.24

GC content: 65.82

Gene sequence:

>822_bases
TTGGGCGCCGGCTTTTTAATTTCCATTGGCGAACCGCTGTTTCACTCCGATCCTGAAGGTCTGATAGGAAAGCAGGCCTT
GCGCGGACGACGCGCCTTTCAAGTGGACGTTGCGATGCCCCCTTCCCTCACGCTCTATGAACTGACCGGCGCGGACGACG
ATCTGCGCTTCAGCCCCCACTGCTGGAAAATCCGCATGGCGCTCCTGCACAAGGGCCTCGAGGCGGATCGCCGTCCCTGG
CGCTTCACGGAAAAGGACGCGATCGCCTTCTCAGGCGGCGCCACCGTGCCGGTGCTCGTCGATGGCGACAAGACAGTTAC
GGATTCCTGGCGCATCGCGCTCCATCTGGAAGAGCACCATGCGGATCGCCCGTCCCTCTTCGGGTCAGAGACGGGGCGTT
CCTATGCCCGCTTCGTCAACAGCTATGCGGACACCGTTCTCGTCGCGCCGCTGCGCCATCTGCTGACGCTCGACATCCTG
GCCCGTGTGCATGAGAAGGACCGCGACTATTTCCGCGCCTCGCGGGAGAAGCGCTTCGGCCAGCCTCTGGAAACCCATGC
CGGCGATCCGGCCGTTCACATCCCGGCCTTCCGTGCGGCCCTGACGCCCGTCCGGACGGTCGTGGCCGAACAGCCGTTCC
TCTCCGGCGCCGCGCCCGCTTATGCGGACTATTGCGCCTTTGGCGCCCTCATGTGGGCCCGCTGCGTCAGCCCGCTGCGC
CTGCTGGAGAAGGACGATCCGGTTTACGGCTGGCGCGAGCGCATGCTCGACGCCTTTGGCGGCCATGCGCGGGCCGCCGC
CTGCACCGAGCAGGACGCGTAA

Upstream 100 bases:

>100_bases
GACGGAAGCGCCCGCGCTGGACCGTCGCCGCGCGGCGGCGCCTGCGCCCCGTCGTCGCGGCCGCTGATCGCGACGCCCGT
TTGAAGACCAGCCGGCGCCT

Downstream 100 bases:

>100_bases
CGATCACGGCCGGGGCAGCGTGATTTCCTGCGCCTCGGCCGTCCCGCCCGCCTTCGCATAGAGGCAGGCGAACGTGCCCC
TGGCATTGTTCATGTGCGCC

Product: glutathione S-transferase

Products: NA

Alternate protein names: Beta-aryl ether cleaving enzyme [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MGAGFLISIGEPLFHSDPEGLIGKQALRGRRAFQVDVAMPPSLTLYELTGADDDLRFSPHCWKIRMALLHKGLEADRRPW
RFTEKDAIAFSGGATVPVLVDGDKTVTDSWRIALHLEEHHADRPSLFGSETGRSYARFVNSYADTVLVAPLRHLLTLDIL
ARVHEKDRDYFRASREKRFGQPLETHAGDPAVHIPAFRAALTPVRTVVAEQPFLSGAAPAYADYCAFGALMWARCVSPLR
LLEKDDPVYGWRERMLDAFGGHARAAACTEQDA

Sequences:

>Translated_273_residues
MGAGFLISIGEPLFHSDPEGLIGKQALRGRRAFQVDVAMPPSLTLYELTGADDDLRFSPHCWKIRMALLHKGLEADRRPW
RFTEKDAIAFSGGATVPVLVDGDKTVTDSWRIALHLEEHHADRPSLFGSETGRSYARFVNSYADTVLVAPLRHLLTLDIL
ARVHEKDRDYFRASREKRFGQPLETHAGDPAVHIPAFRAALTPVRTVVAEQPFLSGAAPAYADYCAFGALMWARCVSPLR
LLEKDDPVYGWRERMLDAFGGHARAAACTEQDA
>Mature_272_residues
GAGFLISIGEPLFHSDPEGLIGKQALRGRRAFQVDVAMPPSLTLYELTGADDDLRFSPHCWKIRMALLHKGLEADRRPWR
FTEKDAIAFSGGATVPVLVDGDKTVTDSWRIALHLEEHHADRPSLFGSETGRSYARFVNSYADTVLVAPLRHLLTLDILA
RVHEKDRDYFRASREKRFGQPLETHAGDPAVHIPAFRAALTPVRTVVAEQPFLSGAAPAYADYCAFGALMWARCVSPLRL
LEKDDPVYGWRERMLDAFGGHARAAACTEQDA

Specific function: Able to degrade various dimeric lignin compounds. Catalyzes the unique and reductive cleavage of arylglycerol-beta- aryl ether [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: NA

Molecular weight: Translated: 30338; Mature: 30207

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAGFLISIGEPLFHSDPEGLIGKQALRGRRAFQVDVAMPPSLTLYELTGADDDLRFSPH
CCCCEEEECCCCHHCCCCCHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCCCCCEECCH
CWKIRMALLHKGLEADRRPWRFTEKDAIAFSGGATVPVLVDGDKTVTDSWRIALHLEEHH
HHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCEECCCEEEEEEEHHHC
ADRPSLFGSETGRSYARFVNSYADTVLVAPLRHLLTLDILARVHEKDRDYFRASREKRFG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QPLETHAGDPAVHIPAFRAALTPVRTVVAEQPFLSGAAPAYADYCAFGALMWARCVSPLR
CCHHHCCCCCEEECHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LLEKDDPVYGWRERMLDAFGGHARAAACTEQDA
HHCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCC
>Mature Secondary Structure 
GAGFLISIGEPLFHSDPEGLIGKQALRGRRAFQVDVAMPPSLTLYELTGADDDLRFSPH
CCCEEEECCCCHHCCCCCHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCCCCCEECCH
CWKIRMALLHKGLEADRRPWRFTEKDAIAFSGGATVPVLVDGDKTVTDSWRIALHLEEHH
HHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCEECCCEEEEEEEHHHC
ADRPSLFGSETGRSYARFVNSYADTVLVAPLRHLLTLDILARVHEKDRDYFRASREKRFG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QPLETHAGDPAVHIPAFRAALTPVRTVVAEQPFLSGAAPAYADYCAFGALMWARCVSPLR
CCHHHCCCCCEEECHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LLEKDDPVYGWRERMLDAFGGHARAAACTEQDA
HHCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1744051; 8495726 [H]