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The map label for this gene is rutF2 [H]

Identifier: 158424783

GI number: 158424783

Start: 3614837

End: 3615388

Strand: Direct

Name: rutF2 [H]

Synonym: AZC_3159

Alternate gene names: 158424783

Gene position: 3614837-3615388 (Clockwise)

Preceding gene: 158424780

Following gene: 158424784

Centisome position: 67.32

GC content: 69.38

Gene sequence:

>552_bases
ATGAACGCCCATTCCGCCATCACGGCCGTCGCGCCCGCCCAGCCGGAACTTGCCGCCACCGCCCTCGCCTTTCGTGCCGG
GATGCGCAAGCTCGCGGGCGCGGTGAGCATTCTCACCGTGGGCGAAGGTGAGCGCCGCACCGGCCTCACCGCCACCTCCG
TTACCTCGCTGTCCGTCGATCCGCCGACGCTCATCATCTGCGTCAACCGTTCGTCCTCCGCCCGGGCGACGCTGATCGAG
GAGGGCCGTTTCGCCATCAACGTGCTGCGGCCGCATCATCAGGCGCTCGCCGACCAGTTCGCCGGGCGCAACGGCGAGAA
GGGGCCGGAGCGCTACAAGGGCGCCGACTGGACACGGCTCGAAACCGGAGCCCCGGTGCTCAACGACGCTCTGGCGGCCT
TCGACTGCTATCTGGAGGAGGCCATCGAGCGGCACTCCCACATCATCGTCATCGGCCGCGTCGCCGCCGCCCGCATCACC
GAGAATGCCGAGCCCCTGCTCTATTGGGCCGGCGCCTATCGCGGCCTCGAAGCGTCGCCAGCCAACGTCTGA

Upstream 100 bases:

>100_bases
TCACGTGATTAGAAGGCGGCATATTCGGGCACGGGCCCGGCACCCCTTCTGCCCGTCGTGACCAGACCCGCCGCCCGCCC
GGCGCTTACACGAGGTTGCC

Downstream 100 bases:

>100_bases
AATCCATCCGGTCCGCGTCACCGCCTCAGGGGTCATGTCATGTCGCACACGAACAACCCGTTTTCCGTGACGCGCCGGCG
CTTCGGGGCGCTGGCCGCCG

Product: actinorhodin polyketide dimerase

Products: NA

Alternate protein names: FMN reductase 2; NAD(P)H:flavin oxidoreductase 2 [H]

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MNAHSAITAVAPAQPELAATALAFRAGMRKLAGAVSILTVGEGERRTGLTATSVTSLSVDPPTLIICVNRSSSARATLIE
EGRFAINVLRPHHQALADQFAGRNGEKGPERYKGADWTRLETGAPVLNDALAAFDCYLEEAIERHSHIIVIGRVAAARIT
ENAEPLLYWAGAYRGLEASPANV

Sequences:

>Translated_183_residues
MNAHSAITAVAPAQPELAATALAFRAGMRKLAGAVSILTVGEGERRTGLTATSVTSLSVDPPTLIICVNRSSSARATLIE
EGRFAINVLRPHHQALADQFAGRNGEKGPERYKGADWTRLETGAPVLNDALAAFDCYLEEAIERHSHIIVIGRVAAARIT
ENAEPLLYWAGAYRGLEASPANV
>Mature_183_residues
MNAHSAITAVAPAQPELAATALAFRAGMRKLAGAVSILTVGEGERRTGLTATSVTSLSVDPPTLIICVNRSSSARATLIE
EGRFAINVLRPHHQALADQFAGRNGEKGPERYKGADWTRLETGAPVLNDALAAFDCYLEEAIERHSHIIVIGRVAAARIT
ENAEPLLYWAGAYRGLEASPANV

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=153, Percent_Identity=29.4117647058824, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 19441; Mature: 19441

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAHSAITAVAPAQPELAATALAFRAGMRKLAGAVSILTVGEGERRTGLTATSVTSLSVD
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEEEEEECC
PPTLIICVNRSSSARATLIEEGRFAINVLRPHHQALADQFAGRNGEKGPERYKGADWTRL
CCEEEEEECCCCCCCEEEEECCCEEEEEECHHHHHHHHHHCCCCCCCCCHHHCCCCCEEE
ETGAPVLNDALAAFDCYLEEAIERHSHIIVIGRVAAARITENAEPLLYWAGAYRGLEASP
CCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHCCCCCCEEEECCCCCCCCCCC
ANV
CCC
>Mature Secondary Structure
MNAHSAITAVAPAQPELAATALAFRAGMRKLAGAVSILTVGEGERRTGLTATSVTSLSVD
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEEEEEECC
PPTLIICVNRSSSARATLIEEGRFAINVLRPHHQALADQFAGRNGEKGPERYKGADWTRL
CCEEEEEECCCCCCCEEEEECCCEEEEEECHHHHHHHHHHCCCCCCCCCHHHCCCCCEEE
ETGAPVLNDALAAFDCYLEEAIERHSHIIVIGRVAAARITENAEPLLYWAGAYRGLEASP
CCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHCCCCCCEEEECCCCCCCCCCC
ANV
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA