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The map label for this gene is nfdA [H]

Identifier: 158424715

GI number: 158424715

Start: 3537394

End: 3539265

Strand: Direct

Name: nfdA [H]

Synonym: AZC_3091

Alternate gene names: 158424715

Gene position: 3537394-3539265 (Clockwise)

Preceding gene: 158424714

Following gene: 158424716

Centisome position: 65.88

GC content: 67.63

Gene sequence:

>1872_bases
GTGACCGCCGACCTCATTCTCCACAACGGCAAGATCACCACGCTCGACCGGACCAATCCGGTGGCGAGCGCCGTCGCCAT
CAAGGACGGCACCTTCCTCGCCGTCGGCACCGATGCCGAGGCCATGGCCCATGCCGGCCCCACGACGCGCAAGGTGGACC
TCAAGGGCCGCAGCGTCCTGCCGGGTCTCATCGACAACCACCTGCACATCATCCGCGGCGGCCTGAATTTCAACATGGAG
CTGCGGTGGGACGGCATGCGCTCGCTGGCCGACGCCATGGACATGCTGAAGCGGCAGGTGGCCGTGACCCCGCCGCCGCA
ATGGGTGCGGGTGGTGGGCGGCTTCACCGAGCACCAGTTCGCCGAAAAGCGCCTGCCGACCATCGCGGAGCTGAACGCGG
TGGCCCCCGAGACGCCGGTCTTCATCCTGCACCTCTATGACCGTGCCCTGCTCAATGCCGCCGCTTTGCGCGCGGTGGGC
TACACGAAGGACACGCCCGAGCCTCCCGGCGGCGAGATCACCCGCGATGCCGCCGGCAATCCCACCGGCCTCCTGCTGGC
GCGGCCCAATGCGGCGATCCTCTATGCGACCCTCGCCAAGGGGCCGAAGCTGCCTTTTGACTATCAGCTCAACTCCACCC
GCCACTTCATGCGCGAGTTGAACCGCCTCGGCGTCACCGGCGCCATCGATGCGGGCGGCGGCTTCCAGAACTATCCCGAC
GATTATGCGGTGATCCAGAAGCTCGCCGACGACAACCAGCTCACCATCCGCCTCGCCTACAACCTCTTCACGCAGAAGCC
GAAGCAGGAGAAGGAGGACTTCCTCAACTGGACGAAGACCTCCAAGTACAAGCAGGGTAGCGACTATTTCCGCCACAACG
GCGCGGGCGAGATGCTGGTCTTCTCCGCCGCCGACTTCGAGGACTTCCGCGAGCCGCGTCCGGACATGCCGCCCGAAATG
GAAGGCGACCTCGAGGGCGTGGTCCGCATCCTCGCCGAGAACCGCTGGCCGTGGCGCCTGCACGCCACCTATGACGAGAC
CATCTCCCGCGCCCTCGACGTGTTCGAGACCGTCAACAAGGACGTGCCGCTCGCGGGACTCAACTGGTTCTTCGACCATG
CGGAGACCATTTCCGAGCGCTCCATCGACCGCATCGCGGCGCTCGGCGGCGGCATCGCCGTGCAGCACCGCATGGCCTAT
CAGGGCGAGTATTTCGTGGAGCGTTACGGCGCCGCCGCCGCCGAGGCGACCCCGCCAGTTGCGCGGATGCTCGACAAGGG
CGTGAAGGTCTCCGCCGGCACCGACGCCACCCGTGTCGCCTCCTATAATCCGTGGGTCTCGCTTTCCTGGCTGGTCACCG
GCCAGACCGTGGGCGGCCTGCGCCTCTATCCCCGCCACAACTGCCTCGACCGCGAGACGGCCCTGCGCATGTGGACGGAG
AAGGTGACGTGGTTCTCCAATGAGGAGGGCCGCAAGGGCCAGATCGTCGCCGGCCAGTTCGCCGACCTCATCGTGCCGGA
CCGTGACTTCTTCTCCTGCCCGGAGGCGGACATCGCCCATATGTCGTCCGACCTGACGATCGTGGGCGGCAAGGTCGTGT
ACGGCGCCGGCGACTTCAAGGCGCTGGACGACACCCCGCTGCCGCCCGCCATGCCCGACTGGTCGCCGGTGCGTACCTTC
AAGGGCTATGCCGCCTGGGGCGAGCCGGAAGGCGCGGGCAAGAACTCGCTGCGCATGGCGGCCGCCTGCGGCTGTGCCAA
TTCCTGCGGCGTGCACGGCCACCAGCATGCCAATGCCTGGTCGAGCCGGCTGCCGGTTTCGGACCTGCGCAGCTTCTGGG
GCGCCCTCGGCTGCGCCTGCTGGGCGGTGTGA

Upstream 100 bases:

>100_bases
CTTCCATCCAGCGCCTCATCGCGCTCCCGCCCGGCCCCGCCGCCTCCAGCTTTCGCACGTCGCCCGCCCCCAATGGGGCG
GCAGCAACGGAGAGACCCGC

Downstream 100 bases:

>100_bases
GGTGGCGCCCCGCGCCACATCGGCTTGGACGGCGGGCCTTCACCGGCTCGCCCTCCTCGCTCTTTGCGCCGCCTATATCC
AGGGGCCGGTGACGAAGCTG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 623; Mature: 622

Protein sequence:

>623_residues
MTADLILHNGKITTLDRTNPVASAVAIKDGTFLAVGTDAEAMAHAGPTTRKVDLKGRSVLPGLIDNHLHIIRGGLNFNME
LRWDGMRSLADAMDMLKRQVAVTPPPQWVRVVGGFTEHQFAEKRLPTIAELNAVAPETPVFILHLYDRALLNAAALRAVG
YTKDTPEPPGGEITRDAAGNPTGLLLARPNAAILYATLAKGPKLPFDYQLNSTRHFMRELNRLGVTGAIDAGGGFQNYPD
DYAVIQKLADDNQLTIRLAYNLFTQKPKQEKEDFLNWTKTSKYKQGSDYFRHNGAGEMLVFSAADFEDFREPRPDMPPEM
EGDLEGVVRILAENRWPWRLHATYDETISRALDVFETVNKDVPLAGLNWFFDHAETISERSIDRIAALGGGIAVQHRMAY
QGEYFVERYGAAAAEATPPVARMLDKGVKVSAGTDATRVASYNPWVSLSWLVTGQTVGGLRLYPRHNCLDRETALRMWTE
KVTWFSNEEGRKGQIVAGQFADLIVPDRDFFSCPEADIAHMSSDLTIVGGKVVYGAGDFKALDDTPLPPAMPDWSPVRTF
KGYAAWGEPEGAGKNSLRMAAACGCANSCGVHGHQHANAWSSRLPVSDLRSFWGALGCACWAV

Sequences:

>Translated_623_residues
MTADLILHNGKITTLDRTNPVASAVAIKDGTFLAVGTDAEAMAHAGPTTRKVDLKGRSVLPGLIDNHLHIIRGGLNFNME
LRWDGMRSLADAMDMLKRQVAVTPPPQWVRVVGGFTEHQFAEKRLPTIAELNAVAPETPVFILHLYDRALLNAAALRAVG
YTKDTPEPPGGEITRDAAGNPTGLLLARPNAAILYATLAKGPKLPFDYQLNSTRHFMRELNRLGVTGAIDAGGGFQNYPD
DYAVIQKLADDNQLTIRLAYNLFTQKPKQEKEDFLNWTKTSKYKQGSDYFRHNGAGEMLVFSAADFEDFREPRPDMPPEM
EGDLEGVVRILAENRWPWRLHATYDETISRALDVFETVNKDVPLAGLNWFFDHAETISERSIDRIAALGGGIAVQHRMAY
QGEYFVERYGAAAAEATPPVARMLDKGVKVSAGTDATRVASYNPWVSLSWLVTGQTVGGLRLYPRHNCLDRETALRMWTE
KVTWFSNEEGRKGQIVAGQFADLIVPDRDFFSCPEADIAHMSSDLTIVGGKVVYGAGDFKALDDTPLPPAMPDWSPVRTF
KGYAAWGEPEGAGKNSLRMAAACGCANSCGVHGHQHANAWSSRLPVSDLRSFWGALGCACWAV
>Mature_622_residues
TADLILHNGKITTLDRTNPVASAVAIKDGTFLAVGTDAEAMAHAGPTTRKVDLKGRSVLPGLIDNHLHIIRGGLNFNMEL
RWDGMRSLADAMDMLKRQVAVTPPPQWVRVVGGFTEHQFAEKRLPTIAELNAVAPETPVFILHLYDRALLNAAALRAVGY
TKDTPEPPGGEITRDAAGNPTGLLLARPNAAILYATLAKGPKLPFDYQLNSTRHFMRELNRLGVTGAIDAGGGFQNYPDD
YAVIQKLADDNQLTIRLAYNLFTQKPKQEKEDFLNWTKTSKYKQGSDYFRHNGAGEMLVFSAADFEDFREPRPDMPPEME
GDLEGVVRILAENRWPWRLHATYDETISRALDVFETVNKDVPLAGLNWFFDHAETISERSIDRIAALGGGIAVQHRMAYQ
GEYFVERYGAAAAEATPPVARMLDKGVKVSAGTDATRVASYNPWVSLSWLVTGQTVGGLRLYPRHNCLDRETALRMWTEK
VTWFSNEEGRKGQIVAGQFADLIVPDRDFFSCPEADIAHMSSDLTIVGGKVVYGAGDFKALDDTPLPPAMPDWSPVRTFK
GYAAWGEPEGAGKNSLRMAAACGCANSCGVHGHQHANAWSSRLPVSDLRSFWGALGCACWAV

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 68458; Mature: 68327

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADLILHNGKITTLDRTNPVASAVAIKDGTFLAVGTDAEAMAHAGPTTRKVDLKGRSVL
CCCEEEEECCEEEEECCCCCCCEEEEEECCCEEEECCCHHHHHCCCCCCEEEECCCCCCC
PGLIDNHLHIIRGGLNFNMELRWDGMRSLADAMDMLKRQVAVTPPPQWVRVVGGFTEHQF
CHHHCCCEEEEECCCEEEEEEEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHH
AEKRLPTIAELNAVAPETPVFILHLYDRALLNAAALRAVGYTKDTPEPPGGEITRDAAGN
HHHHCCCHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCC
PTGLLLARPNAAILYATLAKGPKLPFDYQLNSTRHFMRELNRLGVTGAIDAGGGFQNYPD
CCEEEEECCCCEEEEEEECCCCCCCEEEECCHHHHHHHHHHHCCCCEEEECCCCCCCCCH
DYAVIQKLADDNQLTIRLAYNLFTQKPKQEKEDFLNWTKTSKYKQGSDYFRHNGAGEMLV
HHHHHHHHCCCCCEEEEEEEEHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCEEE
FSAADFEDFREPRPDMPPEMEGDLEGVVRILAENRWPWRLHATYDETISRALDVFETVNK
EECCCHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHCC
DVPLAGLNWFFDHAETISERSIDRIAALGGGIAVQHRMAYQGEYFVERYGAAAAEATPPV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCHHHHHHHCCCCCCCCCHH
ARMLDKGVKVSAGTDATRVASYNPWVSLSWLVTGQTVGGLRLYPRHNCLDRETALRMWTE
HHHHHCCCEEECCCCCCEEECCCCCEEEEEEEECCCCCCEEECCCCCCCCHHHHHHHHHH
KVTWFSNEEGRKGQIVAGQFADLIVPDRDFFSCPEADIAHMSSDLTIVGGKVVYGAGDFK
HHEEECCCCCCCCCEEECCHHHEECCCCCCCCCCCHHHHHCCCCEEEECCEEEECCCCCC
ALDDTPLPPAMPDWSPVRTFKGYAAWGEPEGAGKNSLRMAAACGCANSCGVHGHQHANAW
CCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCEEHHHHHCCCCCCCCCCCCCCCHH
SSRLPVSDLRSFWGALGCACWAV
HHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TADLILHNGKITTLDRTNPVASAVAIKDGTFLAVGTDAEAMAHAGPTTRKVDLKGRSVL
CCEEEEECCEEEEECCCCCCCEEEEEECCCEEEECCCHHHHHCCCCCCEEEECCCCCCC
PGLIDNHLHIIRGGLNFNMELRWDGMRSLADAMDMLKRQVAVTPPPQWVRVVGGFTEHQF
CHHHCCCEEEEECCCEEEEEEEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHH
AEKRLPTIAELNAVAPETPVFILHLYDRALLNAAALRAVGYTKDTPEPPGGEITRDAAGN
HHHHCCCHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCC
PTGLLLARPNAAILYATLAKGPKLPFDYQLNSTRHFMRELNRLGVTGAIDAGGGFQNYPD
CCEEEEECCCCEEEEEEECCCCCCCEEEECCHHHHHHHHHHHCCCCEEEECCCCCCCCCH
DYAVIQKLADDNQLTIRLAYNLFTQKPKQEKEDFLNWTKTSKYKQGSDYFRHNGAGEMLV
HHHHHHHHCCCCCEEEEEEEEHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCEEE
FSAADFEDFREPRPDMPPEMEGDLEGVVRILAENRWPWRLHATYDETISRALDVFETVNK
EECCCHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHCC
DVPLAGLNWFFDHAETISERSIDRIAALGGGIAVQHRMAYQGEYFVERYGAAAAEATPPV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCHHHHHHHCCCCCCCCCHH
ARMLDKGVKVSAGTDATRVASYNPWVSLSWLVTGQTVGGLRLYPRHNCLDRETALRMWTE
HHHHHCCCEEECCCCCCEEECCCCCEEEEEEEECCCCCCEEECCCCCCCCHHHHHHHHHH
KVTWFSNEEGRKGQIVAGQFADLIVPDRDFFSCPEADIAHMSSDLTIVGGKVVYGAGDFK
HHEEECCCCCCCCCEEECCHHHEECCCCCCCCCCCHHHHHCCCCEEEECCEEEECCCCCC
ALDDTPLPPAMPDWSPVRTFKGYAAWGEPEGAGKNSLRMAAACGCANSCGVHGHQHANAW
CCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCEEHHHHHCCCCCCCCCCCCCCCHH
SSRLPVSDLRSFWGALGCACWAV
HHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA