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The map label for this gene is ispDF

Identifier: 158424713

GI number: 158424713

Start: 3535497

End: 3536672

Strand: Direct

Name: ispDF

Synonym: AZC_3089

Alternate gene names: 158424713

Gene position: 3535497-3536672 (Clockwise)

Preceding gene: 158424699

Following gene: 158424714

Centisome position: 65.84

GC content: 70.07

Gene sequence:

>1176_bases
ATGGCTTGCGCTGTTCTTATCGTCGCTGCGGGCCGCGGCACCAGGGCCGGTGGTGCGGTGCCGAAGCAATACCACAGACT
TGGCGGCGAATCTGTGTTGCGGCGCACACTGCTTCGCTTTGCGGAATGCGCAGCCATCGATGTGATTCAGGTGGTCACCC
ATCCCGACGACGCGCCGCTTTATGAGCAGGCGGTCGCGGGCCTGACCAAAGTGCTGCCGCAGGTGGCAGGTGGCGCCACC
CGCCAGCAGTCCGTCCGCGCAGGTCTCGAGGCGCTCGCAGCTTATGGTCCGGAGAGGGTTCTGGTGCAGGACGGCGCCCG
CCCCTTCACGCCGCCGGAGGTGATTGCCCGCGCCATCGCCGCGCTGGACGGAAACACCGCCGCCATCCCCGTCCTGCCCG
TCACGGACACCATCAAGCACGTGGATGCCGCCGGACACGTGACCGGCACCGTGGACCGCGCCATGCTGCGAGCGGTGCAG
ACACCCCAGGCTTTCCGATTCGCGGAGCTGCTCGCCGCCCACCGCGCGGCGGCGGACGCCCGGGTGGCCGACCTCACCGA
CGATGCCTCTGTCATGGAATGGGCGGGGCACACGGTGGCAACCTTTGCCGGAGACAGTGAGAACGTGAAGCTGACCAGCG
CCGAAGACCTCGCCCGTGCCGCCCGCATGCTGGCGGACCTGCCCGACGTGCGCACCGGCACCGGCTTCGACGTCCACGCC
TTCGGCCCCGGCGACCATGTGATGCTGGGCGGCGTCGCCATCCCCTTTGACAGGGGCCTCGACGGCCATTCGGATGCGGA
CGTGGGCCTGCACGCGCTCACCGACGCGGTGCTGGGCGCGCTGGCGGATGGCGACATCGGCGCCCATTTCCCGCCCTCCG
ATCCGCAGTGGAAGGGCGCCACCTCCGACCGCTTCCTCGCCTTCGCCATCGAGCGGGTGCGCGAACGGGGCGGCATCGTC
GCCCATCTGGACCTCACGCTGATCTGCGAGGCGCCCAAGGTCGGGCCCCATCGCGATGCCATCCGCGCCGAGATCGCCCG
CATCTGCGACCTCCCCCTCGGCCGCGTGAGCGTGAAGGCGACCACCACGGAGAAGCTGGGCTTCACTGGCCGGCGCGAGG
GTATCGCCGCCATGGCCTCCGCCACCATCCGCCTGCCGCTGGAGATCGGAGCATGA

Upstream 100 bases:

>100_bases
CCGATGGGGCGGCGGCTCGCTGCCTCCGCATTATCCCTTGCGCCTGCGGCGCGGGCGCTGTTCAACGACGACCAATCGTC
CAGCGGGAACCCTCAAATCA

Downstream 100 bases:

>100_bases
CCGACGAGATCACCGAACGCGCAAGCCGGGTGCTGGACGTCTGCCGGGCGGCGGGCCTGATGGTGGCCACCGCGGAAAGC
TGCACCGGCGGCCTCGTCGT

Product: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MACAVLIVAAGRGTRAGGAVPKQYHRLGGESVLRRTLLRFAECAAIDVIQVVTHPDDAPLYEQAVAGLTKVLPQVAGGAT
RQQSVRAGLEALAAYGPERVLVQDGARPFTPPEVIARAIAALDGNTAAIPVLPVTDTIKHVDAAGHVTGTVDRAMLRAVQ
TPQAFRFAELLAAHRAAADARVADLTDDASVMEWAGHTVATFAGDSENVKLTSAEDLARAARMLADLPDVRTGTGFDVHA
FGPGDHVMLGGVAIPFDRGLDGHSDADVGLHALTDAVLGALADGDIGAHFPPSDPQWKGATSDRFLAFAIERVRERGGIV
AHLDLTLICEAPKVGPHRDAIRAEIARICDLPLGRVSVKATTTEKLGFTGRREGIAAMASATIRLPLEIGA

Sequences:

>Translated_391_residues
MACAVLIVAAGRGTRAGGAVPKQYHRLGGESVLRRTLLRFAECAAIDVIQVVTHPDDAPLYEQAVAGLTKVLPQVAGGAT
RQQSVRAGLEALAAYGPERVLVQDGARPFTPPEVIARAIAALDGNTAAIPVLPVTDTIKHVDAAGHVTGTVDRAMLRAVQ
TPQAFRFAELLAAHRAAADARVADLTDDASVMEWAGHTVATFAGDSENVKLTSAEDLARAARMLADLPDVRTGTGFDVHA
FGPGDHVMLGGVAIPFDRGLDGHSDADVGLHALTDAVLGALADGDIGAHFPPSDPQWKGATSDRFLAFAIERVRERGGIV
AHLDLTLICEAPKVGPHRDAIRAEIARICDLPLGRVSVKATTTEKLGFTGRREGIAAMASATIRLPLEIGA
>Mature_390_residues
ACAVLIVAAGRGTRAGGAVPKQYHRLGGESVLRRTLLRFAECAAIDVIQVVTHPDDAPLYEQAVAGLTKVLPQVAGGATR
QQSVRAGLEALAAYGPERVLVQDGARPFTPPEVIARAIAALDGNTAAIPVLPVTDTIKHVDAAGHVTGTVDRAMLRAVQT
PQAFRFAELLAAHRAAADARVADLTDDASVMEWAGHTVATFAGDSENVKLTSAEDLARAARMLADLPDVRTGTGFDVHAF
GPGDHVMLGGVAIPFDRGLDGHSDADVGLHALTDAVLGALADGDIGAHFPPSDPQWKGATSDRFLAFAIERVRERGGIVA
HLDLTLICEAPKVGPHRDAIRAEIARICDLPLGRVSVKATTTEKLGFTGRREGIAAMASATIRLPLEIGA

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family

Homologues:

Organism=Homo sapiens, GI157412259, Length=237, Percent_Identity=27.0042194092827, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=52.5974025974026, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1789104, Length=224, Percent_Identity=33.4821428571429, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPDF_AZOC5 (A8IBL6)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001526005.1
- ProteinModelPortal:   A8IBL6
- SMR:   A8IBL6
- GeneID:   5688538
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_3089
- HOGENOM:   HBG672839
- OMA:   IVLIHDA
- ProtClustDB:   CLSK2298514
- BioCyc:   ACAU438753:AZC_3089-MONOMER
- HAMAP:   MF_01520
- InterPro:   IPR001228
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00453
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF01128 IspD; PF02542 YgbB; SSF69765 YgbB_synth

EC number: =2.7.7.60; =4.6.1.12

Molecular weight: Translated: 40845; Mature: 40714

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS01295 ISPD; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACAVLIVAAGRGTRAGGAVPKQYHRLGGESVLRRTLLRFAECAAIDVIQVVTHPDDAPL
CCEEEEEEECCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHH
YEQAVAGLTKVLPQVAGGATRQQSVRAGLEALAAYGPERVLVQDGARPFTPPEVIARAIA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
ALDGNTAAIPVLPVTDTIKHVDAAGHVTGTVDRAMLRAVQTPQAFRFAELLAAHRAAADA
HCCCCCEEEEEEECHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
RVADLTDDASVMEWAGHTVATFAGDSENVKLTSAEDLARAARMLADLPDVRTGTGFDVHA
HHHCCCCCHHHHHHCCCEEEEECCCCCCEEEECHHHHHHHHHHHHCCCCCCCCCCCEEEE
FGPGDHVMLGGVAIPFDRGLDGHSDADVGLHALTDAVLGALADGDIGAHFPPSDPQWKGA
ECCCCEEEECCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
TSDRFLAFAIERVRERGGIVAHLDLTLICEAPKVGPHRDAIRAEIARICDLPLGRVSVKA
CCCHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEE
TTTEKLGFTGRREGIAAMASATIRLPLEIGA
ECHHHHCCCCCHHHHHHHHHCEEEEEEEECC
>Mature Secondary Structure 
ACAVLIVAAGRGTRAGGAVPKQYHRLGGESVLRRTLLRFAECAAIDVIQVVTHPDDAPL
CEEEEEEECCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHH
YEQAVAGLTKVLPQVAGGATRQQSVRAGLEALAAYGPERVLVQDGARPFTPPEVIARAIA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
ALDGNTAAIPVLPVTDTIKHVDAAGHVTGTVDRAMLRAVQTPQAFRFAELLAAHRAAADA
HCCCCCEEEEEEECHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
RVADLTDDASVMEWAGHTVATFAGDSENVKLTSAEDLARAARMLADLPDVRTGTGFDVHA
HHHCCCCCHHHHHHCCCEEEEECCCCCCEEEECHHHHHHHHHHHHCCCCCCCCCCCEEEE
FGPGDHVMLGGVAIPFDRGLDGHSDADVGLHALTDAVLGALADGDIGAHFPPSDPQWKGA
ECCCCEEEECCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
TSDRFLAFAIERVRERGGIVAHLDLTLICEAPKVGPHRDAIRAEIARICDLPLGRVSVKA
CCCHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEE
TTTEKLGFTGRREGIAAMASATIRLPLEIGA
ECHHHHCCCCCHHHHHHHHHCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA