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The map label for this gene is dapE [H]

Identifier: 158424681

GI number: 158424681

Start: 3495351

End: 3496730

Strand: Reverse

Name: dapE [H]

Synonym: AZC_3057

Alternate gene names: 158424681

Gene position: 3496730-3495351 (Counterclockwise)

Preceding gene: 158424682

Following gene: 158424680

Centisome position: 65.12

GC content: 69.13

Gene sequence:

>1380_bases
ATGGCCGGTGTGGATGAGGTACTGAACGCAGTGGATGCCGGGCTCGACGCCTCCCTGGCGCGGCTCTCCGATTTCCTGCG
CATCCCCAGCATTTCCACCGATCCGGCCTATGCCGCCGCCTGCCGGCAGGCGGCGGATTTCGCCGCCGAGGCGCTGACCG
GCCTCGGGCTCGCCGCCACCGTGCGCGAGACGGCGGGCCATCCCGTGGTGATCGGCCGCACCGACACGGGCGCCGCCCGC
CGGGTGCTCTTCTACGGCCATTATGACGTGCAGCCGGTGGACCCGCTGGACCTCTGGGACAGCCCGCCCTTTGAGCCGCG
CATCGCCACCGGCACCGACGGCAAGACCCGAATCTATGCGCGCGGCGCCTGCGACGACAAGGGACAGGTCTCCACCTTCC
TCGAGGCGCTGCGGGCGTTGAAGACCGTGCAGGGCAAGCTGCCGGTGGACGTGACCGTGCTGCTGGAAGGGGAGGAGGAG
TGCGGCTCCCCGAGCCTGCCGGCCTTCCTCGCCGCCAACGCGGAAGAACTGAAGGCCGACGTGGCGCTGGTCTGCGACAC
CGGCATGTGGGACCCGCAGACGCCGGCGATCACCACCTCGCTGCGTGGCATCCTGCACACCGAGTTCACCATTACCGGCG
CCGATCGCGATCTGCATTCCGGCCTCTTCGGCGGCGCGGCGCGCAATCCCATCCACGTCCTCGCCCGCATCCTCGCGGCG
GTGCATGACGACACGGGCCACATCACCATTCCGGGCTTCTATGACGACGTGGCCGAGATGCCGCAGGCGGTGCGCGATGC
CTGGAACGCACTCGGTCTGACGCCAGAGGCCTTCCTCGGCCCGGTGGGCCTGTCCGTGGCGGCGGGCGAGAGCGACCGTT
CGCTGATCGAGCAGATCCAGTCCCGCCCCACCTTCGAGGTGAACGGCATCTATGGCGGCTATCAGGGGCAGGGCTCGAAG
ACCATCATTCCCTCGACCGCCACTGCCAAGGTGACGATGCGCCTTGTGGCCAATCAGGATCCGGCCAAGATCGCGGCGGC
CTTCAAGGATTTCGTGCAGGCGCGCCTGCCGGCGGACTGCAAGGTCACCTTCGGCAAGAGCGAGGGCAGCCGCGCCGTGC
TCGTGCCGCCGGACAGCCCGGACCTCGCCAAGGCGCGCACGGCGCTTCAGGCGGAGTGGGGCACGACGCCGGTCGCCATC
GGGTCCGGCGGCTCCATCCCCATCGTCGGCCAGTTCAAGAACGTGCTCGGCATGGACACGCTGCTCATCGGCTTCGGCCT
GGATGACGACCGGGTGCACTCTCCGAACGAGAAATACGACCTCACCTGCTTCCACAAGGGCACGCGGAGCTGGGTGCGGG
TGCTCAGCGCCCTCGCCTGA

Upstream 100 bases:

>100_bases
CCGCGTACCGCGCCGCCTTGTCAGCCGTGCCGCACCGGCCCAGCATTCGCCCCCATGAGTCGCTGCCCATCAGCGGTGGC
GCGAAGGTGAGGAGAAGGCG

Downstream 100 bases:

>100_bases
GGAGCGTCTTACCGCCTTCCCTTGCCGCCTTCCCTTGCGTCCTTCTTTGAACTGTAACGCTTCCGTGTCAGTGAGAAGAC
GCGATAGCCTCTTCGGCCCG

Product: hypothetical protein

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MAGVDEVLNAVDAGLDASLARLSDFLRIPSISTDPAYAAACRQAADFAAEALTGLGLAATVRETAGHPVVIGRTDTGAAR
RVLFYGHYDVQPVDPLDLWDSPPFEPRIATGTDGKTRIYARGACDDKGQVSTFLEALRALKTVQGKLPVDVTVLLEGEEE
CGSPSLPAFLAANAEELKADVALVCDTGMWDPQTPAITTSLRGILHTEFTITGADRDLHSGLFGGAARNPIHVLARILAA
VHDDTGHITIPGFYDDVAEMPQAVRDAWNALGLTPEAFLGPVGLSVAAGESDRSLIEQIQSRPTFEVNGIYGGYQGQGSK
TIIPSTATAKVTMRLVANQDPAKIAAAFKDFVQARLPADCKVTFGKSEGSRAVLVPPDSPDLAKARTALQAEWGTTPVAI
GSGGSIPIVGQFKNVLGMDTLLIGFGLDDDRVHSPNEKYDLTCFHKGTRSWVRVLSALA

Sequences:

>Translated_459_residues
MAGVDEVLNAVDAGLDASLARLSDFLRIPSISTDPAYAAACRQAADFAAEALTGLGLAATVRETAGHPVVIGRTDTGAAR
RVLFYGHYDVQPVDPLDLWDSPPFEPRIATGTDGKTRIYARGACDDKGQVSTFLEALRALKTVQGKLPVDVTVLLEGEEE
CGSPSLPAFLAANAEELKADVALVCDTGMWDPQTPAITTSLRGILHTEFTITGADRDLHSGLFGGAARNPIHVLARILAA
VHDDTGHITIPGFYDDVAEMPQAVRDAWNALGLTPEAFLGPVGLSVAAGESDRSLIEQIQSRPTFEVNGIYGGYQGQGSK
TIIPSTATAKVTMRLVANQDPAKIAAAFKDFVQARLPADCKVTFGKSEGSRAVLVPPDSPDLAKARTALQAEWGTTPVAI
GSGGSIPIVGQFKNVLGMDTLLIGFGLDDDRVHSPNEKYDLTCFHKGTRSWVRVLSALA
>Mature_458_residues
AGVDEVLNAVDAGLDASLARLSDFLRIPSISTDPAYAAACRQAADFAAEALTGLGLAATVRETAGHPVVIGRTDTGAARR
VLFYGHYDVQPVDPLDLWDSPPFEPRIATGTDGKTRIYARGACDDKGQVSTFLEALRALKTVQGKLPVDVTVLLEGEEEC
GSPSLPAFLAANAEELKADVALVCDTGMWDPQTPAITTSLRGILHTEFTITGADRDLHSGLFGGAARNPIHVLARILAAV
HDDTGHITIPGFYDDVAEMPQAVRDAWNALGLTPEAFLGPVGLSVAAGESDRSLIEQIQSRPTFEVNGIYGGYQGQGSKT
IIPSTATAKVTMRLVANQDPAKIAAAFKDFVQARLPADCKVTFGKSEGSRAVLVPPDSPDLAKARTALQAEWGTTPVAIG
SGGSIPIVGQFKNVLGMDTLLIGFGLDDDRVHSPNEKYDLTCFHKGTRSWVRVLSALA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=468, Percent_Identity=29.0598290598291, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI21071039, Length=468, Percent_Identity=27.1367521367521, Blast_Score=185, Evalue=8e-47,
Organism=Homo sapiens, GI271398277, Length=313, Percent_Identity=29.3929712460064, Blast_Score=153, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17563146, Length=470, Percent_Identity=30.8510638297872, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI193211059, Length=478, Percent_Identity=27.6150627615063, Blast_Score=187, Evalue=9e-48,
Organism=Saccharomyces cerevisiae, GI6321159, Length=470, Percent_Identity=27.2340425531915, Blast_Score=185, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6319758, Length=410, Percent_Identity=22.4390243902439, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24585880, Length=459, Percent_Identity=29.1938997821351, Blast_Score=192, Evalue=5e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 48499; Mature: 48368

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGVDEVLNAVDAGLDASLARLSDFLRIPSISTDPAYAAACRQAADFAAEALTGLGLAAT
CCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
VRETAGHPVVIGRTDTGAARRVLFYGHYDVQPVDPLDLWDSPPFEPRIATGTDGKTRIYA
HHHCCCCCEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEE
RGACDDKGQVSTFLEALRALKTVQGKLPVDVTVLLEGEEECGSPSLPAFLAANAEELKAD
CCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECHHHCCCCCCCHHHHCCHHHHCCC
VALVCDTGMWDPQTPAITTSLRGILHTEFTITGADRDLHSGLFGGAARNPIHVLARILAA
EEEEEECCCCCCCCCCHHHHHHHEEEEEEEEECCCCHHHHCCCCCCCCCHHHHHHHHHHH
VHDDTGHITIPGFYDDVAEMPQAVRDAWNALGLTPEAFLGPVGLSVAAGESDRSLIEQIQ
HCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEECCCCHHHHHHHHH
SRPTFEVNGIYGGYQGQGSKTIIPSTATAKVTMRLVANQDPAKIAAAFKDFVQARLPADC
CCCCEEEECEECCCCCCCCCEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCE
KVTFGKSEGSRAVLVPPDSPDLAKARTALQAEWGTTPVAIGSGGSIPIVGQFKNVLGMDT
EEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEECCCCCEEEHHHHHHHHCCCE
LLIGFGLDDDRVHSPNEKYDLTCFHKGTRSWVRVLSALA
EEEEECCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHC
>Mature Secondary Structure 
AGVDEVLNAVDAGLDASLARLSDFLRIPSISTDPAYAAACRQAADFAAEALTGLGLAAT
CCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
VRETAGHPVVIGRTDTGAARRVLFYGHYDVQPVDPLDLWDSPPFEPRIATGTDGKTRIYA
HHHCCCCCEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEE
RGACDDKGQVSTFLEALRALKTVQGKLPVDVTVLLEGEEECGSPSLPAFLAANAEELKAD
CCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECHHHCCCCCCCHHHHCCHHHHCCC
VALVCDTGMWDPQTPAITTSLRGILHTEFTITGADRDLHSGLFGGAARNPIHVLARILAA
EEEEEECCCCCCCCCCHHHHHHHEEEEEEEEECCCCHHHHCCCCCCCCCHHHHHHHHHHH
VHDDTGHITIPGFYDDVAEMPQAVRDAWNALGLTPEAFLGPVGLSVAAGESDRSLIEQIQ
HCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEECCCCHHHHHHHHH
SRPTFEVNGIYGGYQGQGSKTIIPSTATAKVTMRLVANQDPAKIAAAFKDFVQARLPADC
CCCCEEEECEECCCCCCCCCEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCE
KVTFGKSEGSRAVLVPPDSPDLAKARTALQAEWGTTPVAIGSGGSIPIVGQFKNVLGMDT
EEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEECCCCCEEEHHHHHHHHCCCE
LLIGFGLDDDRVHSPNEKYDLTCFHKGTRSWVRVLSALA
EEEEECCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA