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The map label for this gene is dctA

Identifier: 158424638

GI number: 158424638

Start: 3446958

End: 3448298

Strand: Direct

Name: dctA

Synonym: AZC_3014

Alternate gene names: 158424638

Gene position: 3446958-3448298 (Clockwise)

Preceding gene: 158424635

Following gene: 158424642

Centisome position: 64.19

GC content: 65.18

Gene sequence:

>1341_bases
ATGGCACCGGTCATTCCCGCCGCGAGCGCGCCGCACAAGCCGCGCAAGTTCTATCAGCACCTTTACGTCCAGGTTCTGGC
CGCGATCGCCATCGGCATCCTGCTCGGCCATTTCTATCCGGACCTCGGCACCCAGATGAAGCCGCTGGGCGATGCCTTCA
TCAAGCTGGTGAAGATGGTGATCGCGCCGGTGATCTTCCTCACCGTGGTGACCGGCATCGCCGGAATGCGCGATCTGGCA
AAGGTGGGTCGCGTGGCCGGCAAGGCCATGATCTACTTCCTCACCTTCTCCACCCTCGCGCTCATCATCGGCCTCATCAT
CGCCAATGTGGTGCAGCCCGGCGCCGGCATGCACATCGCGCCCGCCTCGCTGGATCCGAAGGCGGTCGCCAGCTACGCCG
CCAAGGCGCATGACGCCTCCATCATCGGCTTCCTGATGAACATCATCCCGGATACGCCGATCAGCGCGCTCGGCTCCGGC
GACATCCTCCAGGTGCTGTTCTTCTCCGTGCTGTTCGGTATTGCGCTGGCCGGCGTGGGCGATCGGGGCGAGCCGGTGAT
GAACGTGCTGGTTTCCGCCTCGCAGGCGATGTTCCGTCTGGTGCACATCCTCATGAAGGCCGCACCCATCGGCGCCTTCG
GCGCCATGGCCTTCACCATCGGCCGCTACGGCATCGGCTCGGTGGCGAACCTCGCCTTCCTCATCCTCACCTTCTACATC
ACATCCTTCCTGTTCGTGGTGGTCGTGCTCGGCCTTGTCGCCCGGTACAACGGCTTCTCCATCTTCGCGCTGCTGCGCTA
CATCAAGGAAGAGCTGCTGCTCGTGCTCGGCACCTCCTCCTCGGAAGCCGCGCTGCCGAGCCTCATCGAGAAGATGGAGC
GGGCCGGCTGCCGCAAGTCGGTGGTGGGCCTCGTCATCCCGACGGGCTACTCCTTCAACCTCGACGGCACCAACATCTAC
ATGACGCTCGCCGCGCTCTTCATCGCGCAGGCAACCGACATCCACCTCAGCCTGGGCGACCAGATCCTGCTGCTGCTCGT
GGCCATGCTCTCCTCCAAGGGCGCGGCCGGCATCACCGGCGCCGGCTTCATCACGCTGGCCGCCACGCTCTCCGTGGTTC
CCACCGTGCCGCTGGCCGGCATGGCGCTGATCCTCGGCATCGACCGCTTCATGTCGGAATGCCGGGCCATCACCAACTTC
ATCGGCAATGCGGTGGCCACCATCGTCGTCGCCCGCTGGGAAGGCGAACTGGACAAGGACAAGCTCGCCGCCGCCCTCTC
CGGCCGCATCTCGGTTGAGGATGACGACCTGCCGATCGAGGCGACCTCTCCGGCCCAGTGA

Upstream 100 bases:

>100_bases
CTTGCGAAATCGTCGGCGGGTAGTTGCCCCCTTCAGCTTCATCAGGTTCGCACGGCGCCCGTGTCCAAAAGGCGCTGATG
GACCGACAGGAGAGACGTCC

Downstream 100 bases:

>100_bases
CCGGATCGAACACCCACGCATGCAACGGCGGCCCTCGGGCCGCCGTTGTCGTTTTCAGCCGTTGATGACCTCCGCCATGG
CGGCCGGGAGCGGGATGCCG

Product: C4-dicarboxylate transporter

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MAPVIPAASAPHKPRKFYQHLYVQVLAAIAIGILLGHFYPDLGTQMKPLGDAFIKLVKMVIAPVIFLTVVTGIAGMRDLA
KVGRVAGKAMIYFLTFSTLALIIGLIIANVVQPGAGMHIAPASLDPKAVASYAAKAHDASIIGFLMNIIPDTPISALGSG
DILQVLFFSVLFGIALAGVGDRGEPVMNVLVSASQAMFRLVHILMKAAPIGAFGAMAFTIGRYGIGSVANLAFLILTFYI
TSFLFVVVVLGLVARYNGFSIFALLRYIKEELLLVLGTSSSEAALPSLIEKMERAGCRKSVVGLVIPTGYSFNLDGTNIY
MTLAALFIAQATDIHLSLGDQILLLLVAMLSSKGAAGITGAGFITLAATLSVVPTVPLAGMALILGIDRFMSECRAITNF
IGNAVATIVVARWEGELDKDKLAAALSGRISVEDDDLPIEATSPAQ

Sequences:

>Translated_446_residues
MAPVIPAASAPHKPRKFYQHLYVQVLAAIAIGILLGHFYPDLGTQMKPLGDAFIKLVKMVIAPVIFLTVVTGIAGMRDLA
KVGRVAGKAMIYFLTFSTLALIIGLIIANVVQPGAGMHIAPASLDPKAVASYAAKAHDASIIGFLMNIIPDTPISALGSG
DILQVLFFSVLFGIALAGVGDRGEPVMNVLVSASQAMFRLVHILMKAAPIGAFGAMAFTIGRYGIGSVANLAFLILTFYI
TSFLFVVVVLGLVARYNGFSIFALLRYIKEELLLVLGTSSSEAALPSLIEKMERAGCRKSVVGLVIPTGYSFNLDGTNIY
MTLAALFIAQATDIHLSLGDQILLLLVAMLSSKGAAGITGAGFITLAATLSVVPTVPLAGMALILGIDRFMSECRAITNF
IGNAVATIVVARWEGELDKDKLAAALSGRISVEDDDLPIEATSPAQ
>Mature_445_residues
APVIPAASAPHKPRKFYQHLYVQVLAAIAIGILLGHFYPDLGTQMKPLGDAFIKLVKMVIAPVIFLTVVTGIAGMRDLAK
VGRVAGKAMIYFLTFSTLALIIGLIIANVVQPGAGMHIAPASLDPKAVASYAAKAHDASIIGFLMNIIPDTPISALGSGD
ILQVLFFSVLFGIALAGVGDRGEPVMNVLVSASQAMFRLVHILMKAAPIGAFGAMAFTIGRYGIGSVANLAFLILTFYIT
SFLFVVVVLGLVARYNGFSIFALLRYIKEELLLVLGTSSSEAALPSLIEKMERAGCRKSVVGLVIPTGYSFNLDGTNIYM
TLAALFIAQATDIHLSLGDQILLLLVAMLSSKGAAGITGAGFITLAATLSVVPTVPLAGMALILGIDRFMSECRAITNFI
GNAVATIVVARWEGELDKDKLAAALSGRISVEDDDLPIEATSPAQ

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family

Homologues:

Organism=Homo sapiens, GI4827012, Length=448, Percent_Identity=26.1160714285714, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI169790839, Length=410, Percent_Identity=28.2926829268293, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI40254478, Length=455, Percent_Identity=25.0549450549451, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI194239697, Length=469, Percent_Identity=24.7334754797441, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI66773030, Length=475, Percent_Identity=23.5789473684211, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI21314632, Length=406, Percent_Identity=25.1231527093596, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI5032093, Length=383, Percent_Identity=27.4151436031332, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI223468564, Length=255, Percent_Identity=31.3725490196078, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI223468566, Length=285, Percent_Identity=29.8245614035088, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI262359914, Length=371, Percent_Identity=27.4932614555256, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI301601644, Length=172, Percent_Identity=28.4883720930233, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1789947, Length=412, Percent_Identity=64.3203883495146, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI1790514, Length=410, Percent_Identity=40.4878048780488, Blast_Score=322, Evalue=2e-89,
Organism=Escherichia coli, GI1788024, Length=412, Percent_Identity=22.5728155339806, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1789473, Length=323, Percent_Identity=22.9102167182663, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17537407, Length=432, Percent_Identity=24.537037037037, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71983099, Length=474, Percent_Identity=24.6835443037975, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71996953, Length=420, Percent_Identity=26.4285714285714, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71983106, Length=474, Percent_Identity=24.6835443037975, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI193206505, Length=444, Percent_Identity=24.7747747747748, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI193206654, Length=460, Percent_Identity=23.2608695652174, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17541374, Length=450, Percent_Identity=22.8888888888889, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24583025, Length=429, Percent_Identity=26.3403263403263, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI17137668, Length=429, Percent_Identity=26.3403263403263, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24583023, Length=429, Percent_Identity=26.3403263403263, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI281360481, Length=504, Percent_Identity=22.8174603174603, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI281360483, Length=504, Percent_Identity=22.8174603174603, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI17137666, Length=504, Percent_Identity=22.8174603174603, Blast_Score=89, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DCTA_AZOC5 (A8IDZ2)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001525930.1
- ProteinModelPortal:   A8IDZ2
- SMR:   A8IDZ2
- GeneID:   5689820
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_3014
- HOGENOM:   HBG739804
- OMA:   AGCKRSV
- ProtClustDB:   CLSK979528
- BioCyc:   ACAU438753:AZC_3014-MONOMER
- HAMAP:   MF_01300
- InterPro:   IPR001991
- InterPro:   IPR018107
- PANTHER:   PTHR11958
- PRINTS:   PR00173

Pfam domain/function: PF00375 SDF

EC number: NA

Molecular weight: Translated: 46893; Mature: 46762

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x21f9eea0)-; HASH(0x215f5ec0)-; HASH(0x21fcedc8)-; HASH(0x223a9f20)-; HASH(0x2233b4bc)-; HASH(0x22316ba0)-; HASH(0x21b96324)-; HASH(0x2255c980)-; HASH(0x2257e7f0)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHCCCEECCCCCCCCCCCCCH
>Mature Secondary Structure 
APVIPAASAPHKPRKFYQHLYVQVLAAIAIGILLGHFYPDLGTQMKPLGDAFIKLVKMV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
IAPVIFLTVVTGIAGMRDLAKVGRVAGKAMIYFLTFSTLALIIGLIIANVVQPGAGMHIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEC
PASLDPKAVASYAAKAHDASIIGFLMNIIPDTPISALGSGDILQVLFFSVLFGIALAGVG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCC
DRGEPVMNVLVSASQAMFRLVHILMKAAPIGAFGAMAFTIGRYGIGSVANLAFLILTFYI
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
TSFLFVVVVLGLVARYNGFSIFALLRYIKEELLLVLGTSSSEAALPSLIEKMERAGCRKS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCHHHH
VVGLVIPTGYSFNLDGTNIYMTLAALFIAQATDIHLSLGDQILLLLVAMLSSKGAAGITG
HEEEEEECCEEECCCCCHHHHHHHHHHHHHHHCEEEECHHHHHHHHHHHHHCCCCCCCCC
AGFITLAATLSVVPTVPLAGMALILGIDRFMSECRAITNFIGNAVATIVVARWEGELDKD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
KLAAALSGRISVEDDDLPIEATSPAQ
HHHHHHHCCCEECCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA