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The map label for this gene is gsiB [H]

Identifier: 158424619

GI number: 158424619

Start: 3423739

End: 3425265

Strand: Reverse

Name: gsiB [H]

Synonym: AZC_2995

Alternate gene names: 158424619

Gene position: 3425265-3423739 (Counterclockwise)

Preceding gene: 158424620

Following gene: 158424618

Centisome position: 63.79

GC content: 64.18

Gene sequence:

>1527_bases
ATGCGTCTCAAATCCCTCATGACTGCCGGCCTGCTGGCGGCGCTGGCCATGGCGCCGGGGCTGGCGCTGGCGAAGCCGCT
CGTGGTCGTCGTGCCGGACAATCTCACCGGCCTCGATCCCATGAACGCCAACGACACCCTGTCCCAGACGGCCATGCGCA
CCGTCTATCAGGGCCTCTTCGGCTTTGATCGCGACATGAAGATCGTGCCCGTGCTCGCCGAGAGCTACGAGGCCAATGAC
AATGCGACGGAATTCACCATCCGCCTGCGCAAGGGCGTGAAGTTCCACGACGGCACCGACTTCAACGCCGCTGCGGTGAA
GGCGAGCCTCGAGCGCCTTGCGAACCCCGACAACAAGCTCTCCCGCCGTTCGCTCATCTCCATGCTGGACCGCGTGGATG
TGGTGGACGACAGCACGGTCAAGATTGTGTTGAAGACGCCCTTCGGCGCGCTCATCAACACGCTCGCCCATCCGGCGCTG
GTGATGATCTCGCCCGCCGCCATGGAAAAGTACGGCAAGGACATCGCCCGCAATCCTGTGGGCACCGGCGCGTTCAAGTT
CAAGCGCTGGGCAGCGGACACCTTCGAGGTGGAGAAGAACGACAATTACTGGAAAGCCGGCTGGCCCAAGGTAGAAGCCG
TGACGATCCGCTCCGTGCCGGAGAACGGCAGCCGCTTTGCCATGCTGCAGGCCGGCGAGGCGCAATATGCGACGGTGTTC
CCGCCGGAACTGGTGCCGGTGGCGGAAAAGCTGCCGAACCTCGAAGTGGTGCGCCGCCCGTCCATCGTCGGCTGGTACGT
GGCGATGAACACGATGAAGAAGCCGTTCAACGACCCGCGCGTGCGCCAGGCGATGAACTATGCCGTGGACAAGGCCGCCT
ATTGCAAGGTGGTCGAGAACGGCTACTGCACGCCCTCGCTCTCTCCGCTGCCCGACCTGCTCGGCTTCTACGAGCCGCAG
AAGCCCTATGCCTATGATGTGGCCAAGGCCAAGGCGCTGCTCGCGGAAGCGGGCTATCCGAACGGGTTCGAGACCGAGAT
CTTCTCCCGCAACAACACCACCTTCATCCGTGCCATGCAGTTCATCCAGCAGCAGCTGGCGCAGGTGGGCGTGAAGGTGA
CGGTCACGCCGCTTGAGGCCGGTGTCGAGGCGCAGCGCATCTGGTCGGTGCAGAAGCCCGAGGATGCGACCGTGCAGATG
CAATACACCGGCTGGTCGTCCTCCACGGGCGACGCCGACTGGGCGCTGCGTCCGCTGCTGTGGGGCAAGGGCTTCCCGCC
GAACCTCTTCAACGTCGCCTACTACAAGAACGAGAAGGTGGACGCCGACTTCGAAGGCGCGTTGGAAACCGCCGATCCGG
CCAAGCGCGGCGCCTTCTATAAGGACGCGCAGCAGCGCATCTGGGAGGATGCGCCGTGGATCTTCCTCGGGGCCTCGCAG
CTCATCACGGTCCAGTCGAAGTCCCTCTCCGGCGTCTACATGCTGCCGGATCGCGGCTTCGTGGTGGAAACCGCCGAGTT
CAAGTGA

Upstream 100 bases:

>100_bases
CTGGTCGAAGTGGGGGAGGGCCATTTCGTGGCCCGTCACCCGATCGGTGACTTCTCCTGAAGTTTTCCAACAACACCTTC
CCAGACAAGAGGAAAACCGG

Downstream 100 bases:

>100_bases
CACTCGGCCGGGGCCGGGAGCGCTGAACGCCCTCCCGCTGCCCCCAACCGCATCCGCCCACCGCCCTCGTGGTGGAGGGC
GGTGCTCCTCCCGGCTGTCC

Product: ABC transporter substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MRLKSLMTAGLLAALAMAPGLALAKPLVVVVPDNLTGLDPMNANDTLSQTAMRTVYQGLFGFDRDMKIVPVLAESYEAND
NATEFTIRLRKGVKFHDGTDFNAAAVKASLERLANPDNKLSRRSLISMLDRVDVVDDSTVKIVLKTPFGALINTLAHPAL
VMISPAAMEKYGKDIARNPVGTGAFKFKRWAADTFEVEKNDNYWKAGWPKVEAVTIRSVPENGSRFAMLQAGEAQYATVF
PPELVPVAEKLPNLEVVRRPSIVGWYVAMNTMKKPFNDPRVRQAMNYAVDKAAYCKVVENGYCTPSLSPLPDLLGFYEPQ
KPYAYDVAKAKALLAEAGYPNGFETEIFSRNNTTFIRAMQFIQQQLAQVGVKVTVTPLEAGVEAQRIWSVQKPEDATVQM
QYTGWSSSTGDADWALRPLLWGKGFPPNLFNVAYYKNEKVDADFEGALETADPAKRGAFYKDAQQRIWEDAPWIFLGASQ
LITVQSKSLSGVYMLPDRGFVVETAEFK

Sequences:

>Translated_508_residues
MRLKSLMTAGLLAALAMAPGLALAKPLVVVVPDNLTGLDPMNANDTLSQTAMRTVYQGLFGFDRDMKIVPVLAESYEAND
NATEFTIRLRKGVKFHDGTDFNAAAVKASLERLANPDNKLSRRSLISMLDRVDVVDDSTVKIVLKTPFGALINTLAHPAL
VMISPAAMEKYGKDIARNPVGTGAFKFKRWAADTFEVEKNDNYWKAGWPKVEAVTIRSVPENGSRFAMLQAGEAQYATVF
PPELVPVAEKLPNLEVVRRPSIVGWYVAMNTMKKPFNDPRVRQAMNYAVDKAAYCKVVENGYCTPSLSPLPDLLGFYEPQ
KPYAYDVAKAKALLAEAGYPNGFETEIFSRNNTTFIRAMQFIQQQLAQVGVKVTVTPLEAGVEAQRIWSVQKPEDATVQM
QYTGWSSSTGDADWALRPLLWGKGFPPNLFNVAYYKNEKVDADFEGALETADPAKRGAFYKDAQQRIWEDAPWIFLGASQ
LITVQSKSLSGVYMLPDRGFVVETAEFK
>Mature_508_residues
MRLKSLMTAGLLAALAMAPGLALAKPLVVVVPDNLTGLDPMNANDTLSQTAMRTVYQGLFGFDRDMKIVPVLAESYEAND
NATEFTIRLRKGVKFHDGTDFNAAAVKASLERLANPDNKLSRRSLISMLDRVDVVDDSTVKIVLKTPFGALINTLAHPAL
VMISPAAMEKYGKDIARNPVGTGAFKFKRWAADTFEVEKNDNYWKAGWPKVEAVTIRSVPENGSRFAMLQAGEAQYATVF
PPELVPVAEKLPNLEVVRRPSIVGWYVAMNTMKKPFNDPRVRQAMNYAVDKAAYCKVVENGYCTPSLSPLPDLLGFYEPQ
KPYAYDVAKAKALLAEAGYPNGFETEIFSRNNTTFIRAMQFIQQQLAQVGVKVTVTPLEAGVEAQRIWSVQKPEDATVQM
QYTGWSSSTGDADWALRPLLWGKGFPPNLFNVAYYKNEKVDADFEGALETADPAKRGAFYKDAQQRIWEDAPWIFLGASQ
LITVQSKSLSGVYMLPDRGFVVETAEFK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=505, Percent_Identity=47.9207920792079, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI1789966, Length=529, Percent_Identity=27.9773156899811, Blast_Score=161, Evalue=7e-41,
Organism=Escherichia coli, GI1787762, Length=481, Percent_Identity=25.3638253638254, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1787495, Length=518, Percent_Identity=26.0617760617761, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789397, Length=488, Percent_Identity=25.4098360655738, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1787551, Length=530, Percent_Identity=25.2830188679245, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789887, Length=532, Percent_Identity=22.9323308270677, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI87081878, Length=347, Percent_Identity=27.3775216138329, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 56241; Mature: 56241

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLKSLMTAGLLAALAMAPGLALAKPLVVVVPDNLTGLDPMNANDTLSQTAMRTVYQGLF
CCHHHHHHHHHHHHHHHCCCHHHHCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHH
GFDRDMKIVPVLAESYEANDNATEFTIRLRKGVKFHDGTDFNAAAVKASLERLANPDNKL
CCCCCCEEEEEEHHCCCCCCCCEEEEEEEECCCEECCCCCCCHHHHHHHHHHHCCCCHHH
SRRSLISMLDRVDVVDDSTVKIVLKTPFGALINTLAHPALVMISPAAMEKYGKDIARNPV
HHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHCCCEEEEECHHHHHHHHHHHHCCCC
GTGAFKFKRWAADTFEVEKNDNYWKAGWPKVEAVTIRSVPENGSRFAMLQAGEAQYATVF
CCCCHHHHHHCCCEEEEECCCCEEECCCCCEEEEEEEECCCCCCEEEEEECCCCCEEEEC
PPELVPVAEKLPNLEVVRRPSIVGWYVAMNTMKKPFNDPRVRQAMNYAVDKAAYCKVVEN
CCCHHHHHHHCCCCEEEECCCEEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
GYCTPSLSPLPDLLGFYEPQKPYAYDVAKAKALLAEAGYPNGFETEIFSRNNTTFIRAMQ
CCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHH
FIQQQLAQVGVKVTVTPLEAGVEAQRIWSVQKPEDATVQMQYTGWSSSTGDADWALRPLL
HHHHHHHHCCCEEEEEEHHCCCCHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCEEHHHH
WGKGFPPNLFNVAYYKNEKVDADFEGALETADPAKRGAFYKDAQQRIWEDAPWIFLGASQ
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHCCCCEEEEECCE
LITVQSKSLSGVYMLPDRGFVVETAEFK
EEEEECCCCCCEEEECCCCEEEEECCCC
>Mature Secondary Structure
MRLKSLMTAGLLAALAMAPGLALAKPLVVVVPDNLTGLDPMNANDTLSQTAMRTVYQGLF
CCHHHHHHHHHHHHHHHCCCHHHHCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHH
GFDRDMKIVPVLAESYEANDNATEFTIRLRKGVKFHDGTDFNAAAVKASLERLANPDNKL
CCCCCCEEEEEEHHCCCCCCCCEEEEEEEECCCEECCCCCCCHHHHHHHHHHHCCCCHHH
SRRSLISMLDRVDVVDDSTVKIVLKTPFGALINTLAHPALVMISPAAMEKYGKDIARNPV
HHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHCCCEEEEECHHHHHHHHHHHHCCCC
GTGAFKFKRWAADTFEVEKNDNYWKAGWPKVEAVTIRSVPENGSRFAMLQAGEAQYATVF
CCCCHHHHHHCCCEEEEECCCCEEECCCCCEEEEEEEECCCCCCEEEEEECCCCCEEEEC
PPELVPVAEKLPNLEVVRRPSIVGWYVAMNTMKKPFNDPRVRQAMNYAVDKAAYCKVVEN
CCCHHHHHHHCCCCEEEECCCEEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
GYCTPSLSPLPDLLGFYEPQKPYAYDVAKAKALLAEAGYPNGFETEIFSRNNTTFIRAMQ
CCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHH
FIQQQLAQVGVKVTVTPLEAGVEAQRIWSVQKPEDATVQMQYTGWSSSTGDADWALRPLL
HHHHHHHHCCCEEEEEEHHCCCCHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCEEHHHH
WGKGFPPNLFNVAYYKNEKVDADFEGALETADPAKRGAFYKDAQQRIWEDAPWIFLGASQ
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHCCCCEEEEECCE
LITVQSKSLSGVYMLPDRGFVVETAEFK
EEEEECCCCCCEEEECCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA