Click here to switch to the map view.

The map label for this gene is iaaA [H]

Identifier: 158424614

GI number: 158424614

Start: 3418919

End: 3419854

Strand: Reverse

Name: iaaA [H]

Synonym: AZC_2990

Alternate gene names: 158424614

Gene position: 3419854-3418919 (Counterclockwise)

Preceding gene: 158424615

Following gene: 158424607

Centisome position: 63.69

GC content: 71.26

Gene sequence:

>936_bases
GTGACGATGCCCGAGTTCAAGCCCGTTCTCGCGCTGCACGGCGGCGCCGGCACCATCCGCCGCTCCGCCATGACGCCAGA
GCGCGAGGCCGCCTATACGCAGGGGCTTCTCACCGCGCTCTCCGCCGGCCGGGCCGTGCTGGAGAGCGGCGGTTCAGCGC
TCGATGCGGTGACGGCCACGGTCATGGCGCTGGAGGACGAGCCGCTGTTCAATGCCGGGCGTGGCGCCGTGCTCACCAGC
GCGGGCACGCTGGAGATGGATGCCGCCATCATGGACGGACGGGACCGCCGGGCCGGCGCGGTGGCGGGCATCTTCGGCCC
GCGCAATCCCATCCTCGCCGCCCGCGCCGTGATGGAAAAGACCGAGCATGTCATGCTGACTGGGAGCGGCGCCAACGCCT
TCTGCCGCGAGAGCGGCCTTGAGATCATGCCGGAGGACTATTTCTTGACCCCCGCCCGGCAGGAGGCACTTGCCCGCGAG
CTGGAGCGCCGCCGCACCGGCGCGCCCGATGATGGCGACGCGGCCCGCAAGCATGGCACGGTGGGTGCGGTGGCGCTCGA
CAGCCATGGCCATCTGGCCGCCGCCACCTCCACCGGCGGCATGACGGCGAAGCTCCCCGGCCGCGTGGGCGACAGCCCTG
TCTTTGGCGCGGGCACATGGGCGGATGACGCCACCTGCGCCGTCTCGGCGACCGGGCATGGCGAATATTTCATCCGCTAT
GCGGTGGGCCATGAGATCGACGCGCGGATGCGCTGGGCCGGGCAGAGCCTGAAGAGGGCCAGCGAGGGCATCGTGCGCGA
GCTGGCGCCGCTGGGCGGCTCGGGCGGCCTCATCGCCGTGGACCGGACGGGGGCTATTTCGCTGCCCTTCAACAGCGAGG
GCATGTACCGCGCCTGGATGCAGGCCGACGGCTCCATGCACACCGCGATTTTCTGA

Upstream 100 bases:

>100_bases
CAGGACGCGACGACGCTCGCCTTCGCCTGCGCCGATGCCGCGGAGGCGGTGGGCTGGATGACGGCGCTCTCCGCCATGTC
TGCCGTCCTGCGCTGAGGAG

Downstream 100 bases:

>100_bases
GAGGTCCGGCGCGCGGGCAGCCCGCGCGCCGTTCGTGGATCAGGGCGCCGGGCTGCCGCGCAGCTGGAAGATGGCGTTCA
CCTTCTCTTCGATCTCCTGC

Product: aspartylglucosaminidase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MTMPEFKPVLALHGGAGTIRRSAMTPEREAAYTQGLLTALSAGRAVLESGGSALDAVTATVMALEDEPLFNAGRGAVLTS
AGTLEMDAAIMDGRDRRAGAVAGIFGPRNPILAARAVMEKTEHVMLTGSGANAFCRESGLEIMPEDYFLTPARQEALARE
LERRRTGAPDDGDAARKHGTVGAVALDSHGHLAAATSTGGMTAKLPGRVGDSPVFGAGTWADDATCAVSATGHGEYFIRY
AVGHEIDARMRWAGQSLKRASEGIVRELAPLGGSGGLIAVDRTGAISLPFNSEGMYRAWMQADGSMHTAIF

Sequences:

>Translated_311_residues
MTMPEFKPVLALHGGAGTIRRSAMTPEREAAYTQGLLTALSAGRAVLESGGSALDAVTATVMALEDEPLFNAGRGAVLTS
AGTLEMDAAIMDGRDRRAGAVAGIFGPRNPILAARAVMEKTEHVMLTGSGANAFCRESGLEIMPEDYFLTPARQEALARE
LERRRTGAPDDGDAARKHGTVGAVALDSHGHLAAATSTGGMTAKLPGRVGDSPVFGAGTWADDATCAVSATGHGEYFIRY
AVGHEIDARMRWAGQSLKRASEGIVRELAPLGGSGGLIAVDRTGAISLPFNSEGMYRAWMQADGSMHTAIF
>Mature_310_residues
TMPEFKPVLALHGGAGTIRRSAMTPEREAAYTQGLLTALSAGRAVLESGGSALDAVTATVMALEDEPLFNAGRGAVLTSA
GTLEMDAAIMDGRDRRAGAVAGIFGPRNPILAARAVMEKTEHVMLTGSGANAFCRESGLEIMPEDYFLTPARQEALAREL
ERRRTGAPDDGDAARKHGTVGAVALDSHGHLAAATSTGGMTAKLPGRVGDSPVFGAGTWADDATCAVSATGHGEYFIRYA
VGHEIDARMRWAGQSLKRASEGIVRELAPLGGSGGLIAVDRTGAISLPFNSEGMYRAWMQADGSMHTAIF

Specific function: Degrades proteins damaged by L-isoaspartyl residue formation (also known as beta-Asp residues). Degrades L- isoaspartyl-containing di- and tripeptides. Acts best on iso-Asp- Leu, followed by iso-Asp-Ala, -His and to a lesser extent iso-Asp- Lys, -Phe and

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=314, Percent_Identity=41.7197452229299, Blast_Score=197, Evalue=9e-51,
Organism=Homo sapiens, GI145275200, Length=314, Percent_Identity=41.7197452229299, Blast_Score=197, Evalue=9e-51,
Organism=Homo sapiens, GI98991769, Length=267, Percent_Identity=31.8352059925094, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI285002251, Length=247, Percent_Identity=34.0080971659919, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI285002253, Length=247, Percent_Identity=31.9838056680162, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1787050, Length=312, Percent_Identity=52.2435897435897, Blast_Score=283, Evalue=8e-78,
Organism=Caenorhabditis elegans, GI32564649, Length=204, Percent_Identity=26.4705882352941, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI18921183, Length=327, Percent_Identity=37.6146788990826, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI21357893, Length=267, Percent_Identity=30.3370786516854, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI221330102, Length=245, Percent_Identity=34.6938775510204, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24652135, Length=245, Percent_Identity=34.6938775510204, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 32401; Mature: 32270

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMPEFKPVLALHGGAGTIRRSAMTPEREAAYTQGLLTALSAGRAVLESGGSALDAVTAT
CCCCCCCCEEEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHE
VMALEDEPLFNAGRGAVLTSAGTLEMDAAIMDGRDRRAGAVAGIFGPRNPILAARAVMEK
EEEECCCCCCCCCCCEEEECCCCEEECHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHC
TEHVMLTGSGANAFCRESGLEIMPEDYFLTPARQEALARELERRRTGAPDDGDAARKHGT
CCEEEEECCCCCHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHCCC
VGAVALDSHGHLAAATSTGGMTAKLPGRVGDSPVFGAGTWADDATCAVSATGHGEYFIRY
EEEEEECCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEE
AVGHEIDARMRWAGQSLKRASEGIVRELAPLGGSGGLIAVDRTGAISLPFNSEGMYRAWM
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEECCCCCCCCEEHEE
QADGSMHTAIF
CCCCCEEEECC
>Mature Secondary Structure 
TMPEFKPVLALHGGAGTIRRSAMTPEREAAYTQGLLTALSAGRAVLESGGSALDAVTAT
CCCCCCCEEEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHE
VMALEDEPLFNAGRGAVLTSAGTLEMDAAIMDGRDRRAGAVAGIFGPRNPILAARAVMEK
EEEECCCCCCCCCCCEEEECCCCEEECHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHC
TEHVMLTGSGANAFCRESGLEIMPEDYFLTPARQEALARELERRRTGAPDDGDAARKHGT
CCEEEEECCCCCHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHCCC
VGAVALDSHGHLAAATSTGGMTAKLPGRVGDSPVFGAGTWADDATCAVSATGHGEYFIRY
EEEEEECCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEE
AVGHEIDARMRWAGQSLKRASEGIVRELAPLGGSGGLIAVDRTGAISLPFNSEGMYRAWM
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEECCCCCCCCEEHEE
QADGSMHTAIF
CCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]