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The map label for this gene is 158424591

Identifier: 158424591

GI number: 158424591

Start: 3388770

End: 3390140

Strand: Reverse

Name: 158424591

Synonym: AZC_2967

Alternate gene names: NA

Gene position: 3390140-3388770 (Counterclockwise)

Preceding gene: 158424592

Following gene: 158424589

Centisome position: 63.13

GC content: 67.25

Gene sequence:

>1371_bases
ATGGCCCGCGACACCACCGATGCGACGCCGATCCGCTCGCGCGACGAGCTCGTTGCCTATCTTGAAGCCGGCAACAAGCC
GGAAGCGGCGTTCCGCATCGGGACCGAGCACGAGAAGATTCCCTTCACCCTCGGCCGGCACGAGCCGGTTCCCTATGAAG
GCGACAAGGGAATCCGGGCGATTCTCGAAGGCATGCGGGACGTGCTCGGCTGGGACCCCATCATGGAAGGCCCGACCATC
ATCGGCCTTGCGGACCCCCGCGCCGGCGGCGCCATCTCGCTGGAGCCCGGCGGCCAGTTCGAGCTTTCGGGCGCGCCGCT
TGCGTCCATCCATGAGACCTGTTCGGAGCTGAACACCCATCTTGCGCAGCTGCGCACGGTGGCCGAGCCGCTCGGCGTCG
GCTTCCTCGGCCTCGGCATGAGCCCCAAATGGAGCCGGGCGGAGACGCCGGTGATGCCCAAGGGCCGCTACAAGATCATG
TCCGGCTACATGCCCAAGGTGGGCAAGCTCGGCCTCGACATGATGTTCCGCACCTGCACCGTGCAGGTGAACCTCGACTT
CTCTTCCGAAGCCGACATGGTGCAGAAGCTGCGCGTCAGCCTCGCGCTCCAGCCCATCGCCACGGCGCTCTTTGCCAATT
CGCCCTTCACCGAAGGGCGGCCGAACGGCTTCCTGACCTTCCGCTCCGAGATCTGGCGCGACACGGACAATGCGCGCTCC
GGAATGCTGCCCTTCGCCTTCGAGGACGGCATGGGCTTCGAGCGCTATGTGGACTATGCGCTCGACGTGCCGATGTATTT
CATCAAGCGCGGCGATGCCTATATCGACGTGGCCGGCTCGTCCTTCCGCGATCTGCTCGCCGGCCGCCATCCGGCGGTGC
CGGGAGAGACCGCAACGCTGGCGGACTGGATCAACCACCTCTCCACCATCTTCCCCGAAGTCCGCCTTAAGCGGTATCTG
GAGATGCGCGGCGCCGACGCGGGTCCGTGGGCCAAGCTCTGCGCGCTGCCGGCCTTCTGGGTCGGGCTGCTCTATGACCG
GCAGAGCCTGGATGCCGCCTGGCAGCTTGCCAAGGACTGGACCGCCGACGAGCGCCAGACCCTGCGTGATGAGGTGCCGC
GGCTCGGGTTGAAGGCCACCATCGGCGGCCGCACCGTGCAGGACATCGCCCGCGACCTTCTTGCGCTTTCGCGCGCGGGT
CTCGTCCGCCGCCGCCAGCTCGACAGCCAGGGCCGGGATGAAACGCGCTTCCTTGCACCGCTGGAAGAGGTGGTCGCCAC
CGGCCGCACGCCTGCCGACGATCTGCTGTCCCGCTATCACGGCGCCTGGCAGGGCTCGGTGGAGCCGGTGTTCGAAGAAC
TCGCCTACTGA

Upstream 100 bases:

>100_bases
CGGGCCGGCCCGCCTGGGCCTGCCGCTTGGTTCTGGACCGGCCGCGTTGCCGCTGGCGCCCTGCCAGCGCGGCGGCTGAG
GGCACGGGGATTAGAGTTTC

Downstream 100 bases:

>100_bases
TCCGGTTTTTGGATCGCGTTCCGAAGGATCGGCGCGGCTATTCCGCCGCGTCGGCCTCGGTGAGATTGTCCAGCCCCTGA
ATGATGTGGTGGGCCAGCGC

Product: glutamate-cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Gamma-Glutamylcysteine Synthetase; Glutamate/Cysteine Ligase Family Protein; Glutamate-Cysteine Ligase GshA; Glutamylcysteine Synthetase; Glutamate-Cysteine Ligase Family 2 Protein; Glutamate-Cysteine Ligase Protein; Glutathione Synthetase; Glutamate-Cysteine Ligase Gsha; Glutamate-Cysteine Ligase Protein; Glutamate-Cysteine Ligase Family Protein; Glutamate Cystein Ligase Protein; Glutamate-Cysteine Ligase GSHA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MARDTTDATPIRSRDELVAYLEAGNKPEAAFRIGTEHEKIPFTLGRHEPVPYEGDKGIRAILEGMRDVLGWDPIMEGPTI
IGLADPRAGGAISLEPGGQFELSGAPLASIHETCSELNTHLAQLRTVAEPLGVGFLGLGMSPKWSRAETPVMPKGRYKIM
SGYMPKVGKLGLDMMFRTCTVQVNLDFSSEADMVQKLRVSLALQPIATALFANSPFTEGRPNGFLTFRSEIWRDTDNARS
GMLPFAFEDGMGFERYVDYALDVPMYFIKRGDAYIDVAGSSFRDLLAGRHPAVPGETATLADWINHLSTIFPEVRLKRYL
EMRGADAGPWAKLCALPAFWVGLLYDRQSLDAAWQLAKDWTADERQTLRDEVPRLGLKATIGGRTVQDIARDLLALSRAG
LVRRRQLDSQGRDETRFLAPLEEVVATGRTPADDLLSRYHGAWQGSVEPVFEELAY

Sequences:

>Translated_456_residues
MARDTTDATPIRSRDELVAYLEAGNKPEAAFRIGTEHEKIPFTLGRHEPVPYEGDKGIRAILEGMRDVLGWDPIMEGPTI
IGLADPRAGGAISLEPGGQFELSGAPLASIHETCSELNTHLAQLRTVAEPLGVGFLGLGMSPKWSRAETPVMPKGRYKIM
SGYMPKVGKLGLDMMFRTCTVQVNLDFSSEADMVQKLRVSLALQPIATALFANSPFTEGRPNGFLTFRSEIWRDTDNARS
GMLPFAFEDGMGFERYVDYALDVPMYFIKRGDAYIDVAGSSFRDLLAGRHPAVPGETATLADWINHLSTIFPEVRLKRYL
EMRGADAGPWAKLCALPAFWVGLLYDRQSLDAAWQLAKDWTADERQTLRDEVPRLGLKATIGGRTVQDIARDLLALSRAG
LVRRRQLDSQGRDETRFLAPLEEVVATGRTPADDLLSRYHGAWQGSVEPVFEELAY
>Mature_455_residues
ARDTTDATPIRSRDELVAYLEAGNKPEAAFRIGTEHEKIPFTLGRHEPVPYEGDKGIRAILEGMRDVLGWDPIMEGPTII
GLADPRAGGAISLEPGGQFELSGAPLASIHETCSELNTHLAQLRTVAEPLGVGFLGLGMSPKWSRAETPVMPKGRYKIMS
GYMPKVGKLGLDMMFRTCTVQVNLDFSSEADMVQKLRVSLALQPIATALFANSPFTEGRPNGFLTFRSEIWRDTDNARSG
MLPFAFEDGMGFERYVDYALDVPMYFIKRGDAYIDVAGSSFRDLLAGRHPAVPGETATLADWINHLSTIFPEVRLKRYLE
MRGADAGPWAKLCALPAFWVGLLYDRQSLDAAWQLAKDWTADERQTLRDEVPRLGLKATIGGRTVQDIARDLLALSRAGL
VRRRQLDSQGRDETRFLAPLEEVVATGRTPADDLLSRYHGAWQGSVEPVFEELAY

Specific function: Unknown

COG id: COG3572

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 50476; Mature: 50345

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDTTDATPIRSRDELVAYLEAGNKPEAAFRIGTEHEKIPFTLGRHEPVPYEGDKGIRA
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCEECCCCCCCCCCCCHHHHH
ILEGMRDVLGWDPIMEGPTIIGLADPRAGGAISLEPGGQFELSGAPLASIHETCSELNTH
HHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEEECCCCCEEECCCCHHHHHHHHHHHHHH
LAQLRTVAEPLGVGFLGLGMSPKWSRAETPVMPKGRYKIMSGYMPKVGKLGLDMMFRTCT
HHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHEEEEEE
VQVNLDFSSEADMVQKLRVSLALQPIATALFANSPFTEGRPNGFLTFRSEIWRDTDNARS
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHCCCCCCCC
GMLPFAFEDGMGFERYVDYALDVPMYFIKRGDAYIDVAGSSFRDLLAGRHPAVPGETATL
CCCCEEECCCCCHHHHHHHHHCCHHHHHHCCCEEEEECCCHHHHHHCCCCCCCCCCHHHH
ADWINHLSTIFPEVRLKRYLEMRGADAGPWAKLCALPAFWVGLLYDRQSLDAAWQLAKDW
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TADERQTLRDEVPRLGLKATIGGRTVQDIARDLLALSRAGLVRRRQLDSQGRDETRFLAP
CCHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LEEVVATGRTPADDLLSRYHGAWQGSVEPVFEELAY
HHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
ARDTTDATPIRSRDELVAYLEAGNKPEAAFRIGTEHEKIPFTLGRHEPVPYEGDKGIRA
CCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCEECCCCCCCCCCCCHHHHH
ILEGMRDVLGWDPIMEGPTIIGLADPRAGGAISLEPGGQFELSGAPLASIHETCSELNTH
HHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEEECCCCCEEECCCCHHHHHHHHHHHHHH
LAQLRTVAEPLGVGFLGLGMSPKWSRAETPVMPKGRYKIMSGYMPKVGKLGLDMMFRTCT
HHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHEEEEEE
VQVNLDFSSEADMVQKLRVSLALQPIATALFANSPFTEGRPNGFLTFRSEIWRDTDNARS
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHCCCCCCCC
GMLPFAFEDGMGFERYVDYALDVPMYFIKRGDAYIDVAGSSFRDLLAGRHPAVPGETATL
CCCCEEECCCCCHHHHHHHHHCCHHHHHHCCCEEEEECCCHHHHHHCCCCCCCCCCHHHH
ADWINHLSTIFPEVRLKRYLEMRGADAGPWAKLCALPAFWVGLLYDRQSLDAAWQLAKDW
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TADERQTLRDEVPRLGLKATIGGRTVQDIARDLLALSRAGLVRRRQLDSQGRDETRFLAP
CCHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LEEVVATGRTPADDLLSRYHGAWQGSVEPVFEELAY
HHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA