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The map label for this gene is Hgd [H]

Identifier: 158424529

GI number: 158424529

Start: 3317795

End: 3318673

Strand: Reverse

Name: Hgd [H]

Synonym: AZC_2905

Alternate gene names: 158424529

Gene position: 3318673-3317795 (Counterclockwise)

Preceding gene: 158424531

Following gene: 158424528

Centisome position: 61.8

GC content: 68.71

Gene sequence:

>879_bases
GTGGCGAACGTTGGTGTGATCGGTCTCGGCAATATGGGGCGCGGGATGGCGCTCTCGCTGAAGCGGGCCGGGGTGAATGT
GGTCGGTTTCGACGCGGTGGAAGCCACCCGCGCGGCGCTCGCGGCGGAAGGCGTCGAGACACTGGCGGATATCGCCGCCC
TTTGCGCCCGCGCCGATGTGCTGGTGCTCTCGCTGCCCACCGCTGCCATCGTGGAAGAGGTGGTGGCCGGCCCCGGCGGC
ATTCTGGCGTCCGGCAAGGCCGGTCTGCTGGTGGTGGACACCTCCACCTCCCATCCCGACACCACCCGCAAACTCGCCAC
GCAGCTCGAAGCTGCCGGCATGGCGATGATCGATGCCCCCGTGAGCGGCGGCCCCAAGGGCGCCATCACCGGCACCATGA
CCATGGTGATCGGCGGCGCGGACGCCGATGTGGCCCGCGCCATGCCGGTGCTGGAAGCCATGAGCGCCACCCGCGTGCAT
GTCGGCCCCGTGGGCGCCGGTCACGTCACCAAGATCATCAACAACCTGCTCTGCGCCGCCCACCTGCTCACCTGTGGCGA
GGCCTTGCGCCTCGCGCAGGAGGCGGGCGTCGAGCCCGCGCGGATGCTGGAAGGCATCAATGCCGGCTCCGGCCGCTCCG
GCGTCAGCCAGGTGAATGCCGGCACGTGGATCCTCAACGGTGCCTTCAATTCCGGCTTCACCATGAAGCTGATGCGCAAG
GACGTGAAGCTCGGCACCGACCTTTTGGCGAGCCTCGGCCTTGAGCTGCCCATGTTCGCCGGCACCGCCGGCATCTGGGC
GGACAGCGCCACGACCATCCCCGACGGCGAGGACTTCAACCGCATCGTCGAGACCGTTTTCCCCGGCAAGAAGAGCTGA

Upstream 100 bases:

>100_bases
CCAAGCGTTGCCTTGGAGTCAACAATACGGAACCGGCCATCTCCTGTCTGCCGCCAAAACAGCCGCGTAGCTTTTCTGGG
CAAGTTTCGAGGAGACGCGC

Downstream 100 bases:

>100_bases
TGCCCGTGACCGCTTTCATTGATCCGCGCCTGCAACAGGCGCTCGCCCCGTTCTGGCCCGGCCTCAAGACCATCGGCTCC
TTCGTGGGCGGTGAGATGCT

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MANVGVIGLGNMGRGMALSLKRAGVNVVGFDAVEATRAALAAEGVETLADIAALCARADVLVLSLPTAAIVEEVVAGPGG
ILASGKAGLLVVDTSTSHPDTTRKLATQLEAAGMAMIDAPVSGGPKGAITGTMTMVIGGADADVARAMPVLEAMSATRVH
VGPVGAGHVTKIINNLLCAAHLLTCGEALRLAQEAGVEPARMLEGINAGSGRSGVSQVNAGTWILNGAFNSGFTMKLMRK
DVKLGTDLLASLGLELPMFAGTAGIWADSATTIPDGEDFNRIVETVFPGKKS

Sequences:

>Translated_292_residues
MANVGVIGLGNMGRGMALSLKRAGVNVVGFDAVEATRAALAAEGVETLADIAALCARADVLVLSLPTAAIVEEVVAGPGG
ILASGKAGLLVVDTSTSHPDTTRKLATQLEAAGMAMIDAPVSGGPKGAITGTMTMVIGGADADVARAMPVLEAMSATRVH
VGPVGAGHVTKIINNLLCAAHLLTCGEALRLAQEAGVEPARMLEGINAGSGRSGVSQVNAGTWILNGAFNSGFTMKLMRK
DVKLGTDLLASLGLELPMFAGTAGIWADSATTIPDGEDFNRIVETVFPGKKS
>Mature_291_residues
ANVGVIGLGNMGRGMALSLKRAGVNVVGFDAVEATRAALAAEGVETLADIAALCARADVLVLSLPTAAIVEEVVAGPGGI
LASGKAGLLVVDTSTSHPDTTRKLATQLEAAGMAMIDAPVSGGPKGAITGTMTMVIGGADADVARAMPVLEAMSATRVHV
GPVGAGHVTKIINNLLCAAHLLTCGEALRLAQEAGVEPARMLEGINAGSGRSGVSQVNAGTWILNGAFNSGFTMKLMRKD
VKLGTDLLASLGLELPMFAGTAGIWADSATTIPDGEDFNRIVETVFPGKKS

Specific function: Catalyzes the conversion of 2-formylglutarate to (S)-2- hydroxymethylglutarate. Has very low activity with (S)-3- hydroxyisobutyrate [H]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=250, Percent_Identity=33.6, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI40556376, Length=267, Percent_Identity=26.2172284644195, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI145693186, Length=258, Percent_Identity=35.6589147286822, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1786719, Length=257, Percent_Identity=33.0739299610895, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1790315, Length=294, Percent_Identity=31.6326530612245, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1789092, Length=297, Percent_Identity=32.996632996633, Blast_Score=102, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17557316, Length=266, Percent_Identity=32.7067669172932, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24655230, Length=263, Percent_Identity=31.9391634980989, Blast_Score=124, Evalue=5e-29,
Organism=Drosophila melanogaster, GI19922568, Length=263, Percent_Identity=31.9391634980989, Blast_Score=124, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.291 [H]

Molecular weight: Translated: 29425; Mature: 29294

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVGVIGLGNMGRGMALSLKRAGVNVVGFDAVEATRAALAAEGVETLADIAALCARADV
CCCCEEEEECCCCCCEEEEEHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
LVLSLPTAAIVEEVVAGPGGILASGKAGLLVVDTSTSHPDTTRKLATQLEAAGMAMIDAP
EEEECCHHHHHHHHHCCCCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCEEEEECC
VSGGPKGAITGTMTMVIGGADADVARAMPVLEAMSATRVHVGPVGAGHVTKIINNLLCAA
CCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHH
HLLTCGEALRLAQEAGVEPARMLEGINAGSGRSGVSQVNAGTWILNGAFNSGFTMKLMRK
HHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHCCCCEEEEECCCCCCCHHHHHHH
DVKLGTDLLASLGLELPMFAGTAGIWADSATTIPDGEDFNRIVETVFPGKKS
HHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ANVGVIGLGNMGRGMALSLKRAGVNVVGFDAVEATRAALAAEGVETLADIAALCARADV
CCCEEEEECCCCCCEEEEEHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
LVLSLPTAAIVEEVVAGPGGILASGKAGLLVVDTSTSHPDTTRKLATQLEAAGMAMIDAP
EEEECCHHHHHHHHHCCCCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCEEEEECC
VSGGPKGAITGTMTMVIGGADADVARAMPVLEAMSATRVHVGPVGAGHVTKIINNLLCAA
CCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHH
HLLTCGEALRLAQEAGVEPARMLEGINAGSGRSGVSQVNAGTWILNGAFNSGFTMKLMRK
HHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHCCCCEEEEECCCCCCCHHHHHHH
DVKLGTDLLASLGLELPMFAGTAGIWADSATTIPDGEDFNRIVETVFPGKKS
HHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA