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The map label for this gene is trmFO

Identifier: 158424513

GI number: 158424513

Start: 3300730

End: 3302145

Strand: Direct

Name: trmFO

Synonym: AZC_2889

Alternate gene names: 158424513

Gene position: 3300730-3302145 (Clockwise)

Preceding gene: 158424512

Following gene: 158424514

Centisome position: 61.47

GC content: 68.5

Gene sequence:

>1416_bases
ATGAGCCAGACGTCCCTCCAGCCGGTCCATGTGGTCGGCGGCGGCCTTGCCGGTTCCGAAGCGGCCTTCCAGCTCGCCGA
ACGCGGCGTGCCGGTGGTGCTGCACGAGATGCGCCCGGTGCGCGGCACGGAGGCCCATCACACGGACGGCCTTGCGGAAC
TCGTCTGCTCCAATTCCTTCCGCTCCGATGACGCCCAGACCAATGCGGTGGGCGTGCTGCACGCGGAGATGCGCCGCGCC
GGCTCGCTCATCCTGCGCTGCGCCGATGCCAATCAGGTCCCTGCCGGCGGAGCGCTGGCGGTGGATCGCGACGGTTTCTC
GCAGGCGGTCACCGCCGCTCTGGCGGAACACCCCCTGGTGGAGATTCGCCGCGAGGAAGTCACCGCCATCCCGCCGGAGG
AGTGGGACAACGTCATCATCGCCACCGGCCCCCTCACCTCGCCCGCGCTCGCGCAGGCGGTGCTGGAGCTGACCGGCGAA
AGCGCCCTCGCCTTCTTCGACGCCATCGCCCCGATCGTGCATCTGGACAGCATCGATTTGTCCAAGGCCTGGTTCCAGTC
GCGCTATGACAAGGTGGGCCCCGGCGGCACGGGCGCGGATTACATCAACTGCCCGCTGGACAAGGAACAGTATGAGGCCT
TCGTCGATGCGCTGCTGGCGTCCGAGAAGGTGCCGCTGCGCGATTTCGAGGCCAAGACGCCCTATTTCGACGGCTGCCTG
CCCATCGAAGTGATGGCCGAACGCGGCCGCGAGACGCTCCGCTTCGGGCCCCTGAAGCCGGTCGGCCTCACCAATCCGCA
CAATCCGGATGTGAAGCCCCACGCCGTCATCCAGCTGCGGCAGGATAACAAGCTGGGCACGCTCTATAATCTGGTCGGCT
TCCAGACCAAGATGCGCCATGGCGAGCAGGTGCGCGTGTTCCGCACCATTCCCGGCCTTGAGAATGCGGAGTTCGCCCGG
CTCGGCGGCCTGCATCGCAACACCTATCTGAACTCGCCTCGCCTGCTTGACGGCACCCTGCGCCTGAAGGCGGCGCCCCG
CCTGCGCTTCGCCGGCCAGATCACCGGTTGCGAAGGCTATGTGGAAAGCGCCGCCGTGGGCCTCATGGCCGGCCGCTTCG
CGGCCGCAGAACGCCGGGGCGAAGCCCTGGTGCCGCCGCCCGCCACCACAGCCCATGGGGCGCTCCTCGCCCACATCACC
GGCGGGCATATCGAGACGGTGGATGCCGGCCCGCGCTCCTTCCAGCCCATGAACGTCAATTTCGGCCTGTTTCCGCAGCT
CGACGTGAGCCCCAAGGGGGCCGACGGCAAGCGCCTGCGCGGGTCGGAGAAGACGCTCGCCAAGAAGCGCCTGCTGGCGG
AACGGGCACTTTCCGACATGAGCGCGTGGCTCGACACGCCCGCCGCGGCGGCGTAA

Upstream 100 bases:

>100_bases
CGCGCGAGCGCACCGACCGCCTGCCCGCAGGCCGGTCTGTGAACGCGCTCCAAGCCGGGCCTGACCCACCCGCCCGTCCT
GCCCGCTCAAGTGAGGATGC

Downstream 100 bases:

>100_bases
GAGGCGCCATGCCGAGGCTGAAACGCTCCGTGCCGCCGGTCGAACACCAGGCGGACCCTTCCCGCTTCACCGTGGACACG
GTGCTGAAGCGCGACGTGTT

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSQTSLQPVHVVGGGLAGSEAAFQLAERGVPVVLHEMRPVRGTEAHHTDGLAELVCSNSFRSDDAQTNAVGVLHAEMRRA
GSLILRCADANQVPAGGALAVDRDGFSQAVTAALAEHPLVEIRREEVTAIPPEEWDNVIIATGPLTSPALAQAVLELTGE
SALAFFDAIAPIVHLDSIDLSKAWFQSRYDKVGPGGTGADYINCPLDKEQYEAFVDALLASEKVPLRDFEAKTPYFDGCL
PIEVMAERGRETLRFGPLKPVGLTNPHNPDVKPHAVIQLRQDNKLGTLYNLVGFQTKMRHGEQVRVFRTIPGLENAEFAR
LGGLHRNTYLNSPRLLDGTLRLKAAPRLRFAGQITGCEGYVESAAVGLMAGRFAAAERRGEALVPPPATTAHGALLAHIT
GGHIETVDAGPRSFQPMNVNFGLFPQLDVSPKGADGKRLRGSEKTLAKKRLLAERALSDMSAWLDTPAAAA

Sequences:

>Translated_471_residues
MSQTSLQPVHVVGGGLAGSEAAFQLAERGVPVVLHEMRPVRGTEAHHTDGLAELVCSNSFRSDDAQTNAVGVLHAEMRRA
GSLILRCADANQVPAGGALAVDRDGFSQAVTAALAEHPLVEIRREEVTAIPPEEWDNVIIATGPLTSPALAQAVLELTGE
SALAFFDAIAPIVHLDSIDLSKAWFQSRYDKVGPGGTGADYINCPLDKEQYEAFVDALLASEKVPLRDFEAKTPYFDGCL
PIEVMAERGRETLRFGPLKPVGLTNPHNPDVKPHAVIQLRQDNKLGTLYNLVGFQTKMRHGEQVRVFRTIPGLENAEFAR
LGGLHRNTYLNSPRLLDGTLRLKAAPRLRFAGQITGCEGYVESAAVGLMAGRFAAAERRGEALVPPPATTAHGALLAHIT
GGHIETVDAGPRSFQPMNVNFGLFPQLDVSPKGADGKRLRGSEKTLAKKRLLAERALSDMSAWLDTPAAAA
>Mature_470_residues
SQTSLQPVHVVGGGLAGSEAAFQLAERGVPVVLHEMRPVRGTEAHHTDGLAELVCSNSFRSDDAQTNAVGVLHAEMRRAG
SLILRCADANQVPAGGALAVDRDGFSQAVTAALAEHPLVEIRREEVTAIPPEEWDNVIIATGPLTSPALAQAVLELTGES
ALAFFDAIAPIVHLDSIDLSKAWFQSRYDKVGPGGTGADYINCPLDKEQYEAFVDALLASEKVPLRDFEAKTPYFDGCLP
IEVMAERGRETLRFGPLKPVGLTNPHNPDVKPHAVIQLRQDNKLGTLYNLVGFQTKMRHGEQVRVFRTIPGLENAEFARL
GGLHRNTYLNSPRLLDGTLRLKAAPRLRFAGQITGCEGYVESAAVGLMAGRFAAAERRGEALVPPPATTAHGALLAHITG
GHIETVDAGPRSFQPMNVNFGLFPQLDVSPKGADGKRLRGSEKTLAKKRLLAERALSDMSAWLDTPAAAA

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_AZOC5 (A8ID68)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001525805.1
- ProteinModelPortal:   A8ID68
- SMR:   A8ID68
- GeneID:   5688269
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_2889
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- BioCyc:   ACAU438753:AZC_2889-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 50522; Mature: 50391

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTSLQPVHVVGGGLAGSEAAFQLAERGVPVVLHEMRPVRGTEAHHTDGLAELVCSNSF
CCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHCCCC
RSDDAQTNAVGVLHAEMRRAGSLILRCADANQVPAGGALAVDRDGFSQAVTAALAEHPLV
CCCCCCCCEEEEHHHHHHHCCCEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHCCHH
EIRREEVTAIPPEEWDNVIIATGPLTSPALAQAVLELTGESALAFFDAIAPIVHLDSIDL
HHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHEECCCCH
SKAWFQSRYDKVGPGGTGADYINCPLDKEQYEAFVDALLASEKVPLRDFEAKTPYFDGCL
HHHHHHHHHCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
PIEVMAERGRETLRFGPLKPVGLTNPHNPDVKPHAVIQLRQDNKLGTLYNLVGFQTKMRH
HHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCC
GEQVRVFRTIPGLENAEFARLGGLHRNTYLNSPRLLDGTLRLKAAPRLRFAGQITGCEGY
CCEEEEEEECCCCCCCCHHHHCCCHHCCCCCCCCEECCEEEEECCCCEEECEEECCCHHH
VESAAVGLMAGRFAAAERRGEALVPPPATTAHGALLAHITGGHIETVDAGPRSFQPMNVN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCC
FGLFPQLDVSPKGADGKRLRGSEKTLAKKRLLAERALSDMSAWLDTPAAAA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SQTSLQPVHVVGGGLAGSEAAFQLAERGVPVVLHEMRPVRGTEAHHTDGLAELVCSNSF
CCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHCCCC
RSDDAQTNAVGVLHAEMRRAGSLILRCADANQVPAGGALAVDRDGFSQAVTAALAEHPLV
CCCCCCCCEEEEHHHHHHHCCCEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHCCHH
EIRREEVTAIPPEEWDNVIIATGPLTSPALAQAVLELTGESALAFFDAIAPIVHLDSIDL
HHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHEECCCCH
SKAWFQSRYDKVGPGGTGADYINCPLDKEQYEAFVDALLASEKVPLRDFEAKTPYFDGCL
HHHHHHHHHCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
PIEVMAERGRETLRFGPLKPVGLTNPHNPDVKPHAVIQLRQDNKLGTLYNLVGFQTKMRH
HHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCC
GEQVRVFRTIPGLENAEFARLGGLHRNTYLNSPRLLDGTLRLKAAPRLRFAGQITGCEGY
CCEEEEEEECCCCCCCCHHHHCCCHHCCCCCCCCEECCEEEEECCCCEEECEEECCCHHH
VESAAVGLMAGRFAAAERRGEALVPPPATTAHGALLAHITGGHIETVDAGPRSFQPMNVN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCC
FGLFPQLDVSPKGADGKRLRGSEKTLAKKRLLAERALSDMSAWLDTPAAAA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA