Click here to switch to the map view.

The map label for this gene is braE [H]

Identifier: 158424494

GI number: 158424494

Start: 3267580

End: 3268569

Strand: Reverse

Name: braE [H]

Synonym: AZC_2870

Alternate gene names: 158424494

Gene position: 3268569-3267580 (Counterclockwise)

Preceding gene: 158424495

Following gene: 158424493

Centisome position: 60.87

GC content: 67.58

Gene sequence:

>990_bases
ATGTCACAGACGTCCTCTCCCATACTCTCCGGCCGCAGCCTTGCCCTCGGGGCCGGCCTCGTGGTCGTCGCCCTGCTGCC
GCTGGCGGCGGGCGCCAGCCCCTACATGCTGGGGCTGCTCATCTCCGCCCTCATCCTCGCCGGTCTCGCCCTCTCCTGGG
CGCTGCTCGGCAATCTCGGCGGCATGGTCTCGTTCGGCCATGCAGCCTTCTTCGGGGTCGGCTCCTATGCCTCGGCGCTG
CTGGCGGCGCGGCTCGGCGTGCCGGTGCTGCTGGCGATCCCGCTGGCCGGCCTTATCGCAACCATTTCCTCGCTCGCCAT
GCTGCCGGCGCTGCGGCTCTCGGGGCCCTATTTCGCCCTCGCCATCCTCGCCTATGCGCAGATCTTCCGCATTCTCGCCA
CCGAGGCCACCTGGCTGACCGGTGGCGCCGCGGGCCTCCAGCGCTATCCCGGCCTGCCCACGCTGTTCGGCTTCAATCTC
GCCGAGCGCCACGGCAGCTACGTTCTGGTGCTGGCGATGGTGGTCATCTGCGCCATCATCTACGTGGCCATCCGCCGGAG
CCATGTGGGCATCGCGCTCCGGGCCATCGCGGAGAGCGAGGATGCGACCCGGGTCGTGGGCGTGAACAGCACCCTGCTCA
AGACCTGGATGCTGATGCTCTCGGCCTTCATCGCCGGCATGTTCGGCGCGCTGAACGCGCACATCATCGGCTTCCTGGAA
CCGGATTATGCCTTCTCCGGCATCTGGAGCGTGATGCCGATCATCGCGGCGGTGTTCGGCGGCTATCGCACGGTGCTCGG
GCCGGTGCTCGGCGCCATCGTGATCTACATGTTCGATCAGGTCGTGGCCAAGAGCCTCATCCCCGTCGGCCACCAGATCC
TGCTCGGCATCCTCCTCGCCATCATGGTGCTGGTGGCGCCGAACGGCCTTGCCGGTCTTCTGTCCCGTTTCTTCTCCCGC
AGCGGAGGCAAGCATCATGCTCGCGCTTGA

Upstream 100 bases:

>100_bases
CTTCAGGAACTCGCCGGCATGGTGCTGTTCCTGCTGGTCCTCTTCCTCATGCCGAACGGCCTGTTCGGCGCCAGCGCCCG
CCGCGGCTGAGAGGCGAGCC

Downstream 100 bases:

>100_bases
GGGCGTCTCCGTCCGCTTCGGCGGCCTGCTCGCGCTGTCCAACATCAGCTTCTCGGTCGAGAAGGGCGAGATTCTCGGCC
TTGTCGGCCCCAATGGCGCC

Product: branched-chain amino acid ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MSQTSSPILSGRSLALGAGLVVVALLPLAAGASPYMLGLLISALILAGLALSWALLGNLGGMVSFGHAAFFGVGSYASAL
LAARLGVPVLLAIPLAGLIATISSLAMLPALRLSGPYFALAILAYAQIFRILATEATWLTGGAAGLQRYPGLPTLFGFNL
AERHGSYVLVLAMVVICAIIYVAIRRSHVGIALRAIAESEDATRVVGVNSTLLKTWMLMLSAFIAGMFGALNAHIIGFLE
PDYAFSGIWSVMPIIAAVFGGYRTVLGPVLGAIVIYMFDQVVAKSLIPVGHQILLGILLAIMVLVAPNGLAGLLSRFFSR
SGGKHHARA

Sequences:

>Translated_329_residues
MSQTSSPILSGRSLALGAGLVVVALLPLAAGASPYMLGLLISALILAGLALSWALLGNLGGMVSFGHAAFFGVGSYASAL
LAARLGVPVLLAIPLAGLIATISSLAMLPALRLSGPYFALAILAYAQIFRILATEATWLTGGAAGLQRYPGLPTLFGFNL
AERHGSYVLVLAMVVICAIIYVAIRRSHVGIALRAIAESEDATRVVGVNSTLLKTWMLMLSAFIAGMFGALNAHIIGFLE
PDYAFSGIWSVMPIIAAVFGGYRTVLGPVLGAIVIYMFDQVVAKSLIPVGHQILLGILLAIMVLVAPNGLAGLLSRFFSR
SGGKHHARA
>Mature_328_residues
SQTSSPILSGRSLALGAGLVVVALLPLAAGASPYMLGLLISALILAGLALSWALLGNLGGMVSFGHAAFFGVGSYASALL
AARLGVPVLLAIPLAGLIATISSLAMLPALRLSGPYFALAILAYAQIFRILATEATWLTGGAAGLQRYPGLPTLFGFNLA
ERHGSYVLVLAMVVICAIIYVAIRRSHVGIALRAIAESEDATRVVGVNSTLLKTWMLMLSAFIAGMFGALNAHIIGFLEP
DYAFSGIWSVMPIIAAVFGGYRTVLGPVLGAIVIYMFDQVVAKSLIPVGHQILLGILLAIMVLVAPNGLAGLLSRFFSRS
GGKHHARA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=279, Percent_Identity=29.3906810035842, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 34136; Mature: 34004

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTSSPILSGRSLALGAGLVVVALLPLAAGASPYMLGLLISALILAGLALSWALLGNLG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
GMVSFGHAAFFGVGSYASALLAARLGVPVLLAIPLAGLIATISSLAMLPALRLSGPYFAL
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
AILAYAQIFRILATEATWLTGGAAGLQRYPGLPTLFGFNLAERHGSYVLVLAMVVICAII
HHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHCCCHHHHCCCHHHHHHHHHHHHHH
YVAIRRSHVGIALRAIAESEDATRVVGVNSTLLKTWMLMLSAFIAGMFGALNAHIIGFLE
HHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
PDYAFSGIWSVMPIIAAVFGGYRTVLGPVLGAIVIYMFDQVVAKSLIPVGHQILLGILLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
IMVLVAPNGLAGLLSRFFSRSGGKHHARA
HHHHHCCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SQTSSPILSGRSLALGAGLVVVALLPLAAGASPYMLGLLISALILAGLALSWALLGNLG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
GMVSFGHAAFFGVGSYASALLAARLGVPVLLAIPLAGLIATISSLAMLPALRLSGPYFAL
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
AILAYAQIFRILATEATWLTGGAAGLQRYPGLPTLFGFNLAERHGSYVLVLAMVVICAII
HHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHCCCHHHHCCCHHHHHHHHHHHHHH
YVAIRRSHVGIALRAIAESEDATRVVGVNSTLLKTWMLMLSAFIAGMFGALNAHIIGFLE
HHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
PDYAFSGIWSVMPIIAAVFGGYRTVLGPVLGAIVIYMFDQVVAKSLIPVGHQILLGILLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
IMVLVAPNGLAGLLSRFFSRSGGKHHARA
HHHHHCCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]