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The map label for this gene is shiA [H]

Identifier: 158424412

GI number: 158424412

Start: 3168335

End: 3169780

Strand: Reverse

Name: shiA [H]

Synonym: AZC_2788

Alternate gene names: 158424412

Gene position: 3169780-3168335 (Counterclockwise)

Preceding gene: 158424414

Following gene: 158424411

Centisome position: 59.03

GC content: 65.84

Gene sequence:

>1446_bases
ATGAACAATACCGGCGAACCCTCGCCGGAGCGGCTCCGCGCGTCAGCGCCGGATGCCCGCCTGCCCGGACGCAGAAGCCG
GACAGGCAACGACCCCGCGTGCGCGTCCCAACGACCTTCCAGAGAGGCATTCATGACGTATACCGGCACCCTGGCGCCGC
CCTCGGCGGCAGTTGAACGCAACGAGATGCGCCGCATCGTCTGGTCGTCCGTCATCGGCACGGCCGTCGAGTGGTACGAC
TTCCTCATCTATGCGGCAGCGACCGCCCTCGTCTTCAACAAGCTGTTCTTCCCGGTGGGCGATCCGGCACTCGCCACCAT
CGCCGCCTTCGGCACCTATGCGGTGGGCTTCCTGGCCCGGCCGCTCGGCGCTGCCATTTTCGGCCATTATGGTGACAAGC
TGGGCCGCAAGGCCATGCTCGCCACCACCATCGTCATCATGGGGCTCGGCACCTTCTGCATCGGCCTCCTGCCGACTTAC
GAGCAGATCGGCATCGCCGCTCCCATTCTGCTGCTGATCCTGCGCTTTCTCCAGGGCATCGGCATCGGCGGCGAGTGGGG
CGGGGCGGTGCTCATGGTGGTGGAGAATGCGCCGGCCAAGCGCCGCGGCCTCTTTGGCTCCATGGTGCAGGTGGGCAACC
CCATCGGCAATCTCGCCGCCATCGGCATCTTCGCCCTCGTCTCGCAACTGCCCGACAGCGCCTTCCTGTCCTGGGGGTGG
CGGCTGCCGTTCCTGCTCTCCATCGTCCTCATCGCGGTCGGTCTCTTCATCCGGCTGAAGCTGGACGAGACGCCCGCCTT
CCGCGAGATGAAGGCGAAGAACGCCTGTGCCCAGGCGCCCCTCGTCGAGATCTTCCGCGATCACCGCCGCCCCTTCTTCA
CCGCCGTGGGCCTGAAGATCGCCGAAATCTCCTACGCCTCCATCGCCGGCGTCTTCGTCATCTCCTATGCCACCGTCCAG
CTCGGCATGCCGCGCTCCACCATTCTCAACGGCGTCTGGCTCTCCTCCTTCATCGCGCTGTTCACCATCCCGCTGTTCGG
CTGGATGTCGGACAAACTCGGTCGAAAGACCATGTTCTTCCTGAGCTGCCTGTTCTGCGCCGGCTTCGCCTTTCCCATGT
TCTCGCTGCTTGAGACCCGCGATCCGGCCATCGTCACGGCGACCATCGTGGTGGCCATCTCCTGCGGCCAGATGGTGATG
TTCGGCATCGGCGCCCCCTGGTACTCGGAGCTGTTCACGGCGCGGCTGCGCTATTCCGGCGCCTCGCTGGGCTTCCAGAT
CGGGGCGGCGCTGTCCGGCGGGCTCACACCCTTCATTGCGGCGGCGCTGATGCAGTGGAGCGGCGGCGGCACCTGGCCCA
TCTCGCTCTATCTCATCGGCTGTGCGCTCATCACCGCTTTCGCTACCTTCATGGCGCCGGAGACGGCGCGCAAGGAAATG
ATCTGA

Upstream 100 bases:

>100_bases
CGGTGGCGCCCTTGTACCGATCATCCAGCGCAAAGGCGACCGGTGCTGCCGGCCGCCCCGTTCTCGGGCAGGCACCGGTC
GCCGGCTCAAGCGTGATCAG

Downstream 100 bases:

>100_bases
CGCTTCCCGGTCCCGGGAGAGCGATCTCCCGGGCCTCGATCCGAAAGGCCCAAGGACATGCTCAGTCTGCAACCCGCCAT
CGAGACGCCGGCCCCCGCTT

Product: metabolite transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MNNTGEPSPERLRASAPDARLPGRRSRTGNDPACASQRPSREAFMTYTGTLAPPSAAVERNEMRRIVWSSVIGTAVEWYD
FLIYAAATALVFNKLFFPVGDPALATIAAFGTYAVGFLARPLGAAIFGHYGDKLGRKAMLATTIVIMGLGTFCIGLLPTY
EQIGIAAPILLLILRFLQGIGIGGEWGGAVLMVVENAPAKRRGLFGSMVQVGNPIGNLAAIGIFALVSQLPDSAFLSWGW
RLPFLLSIVLIAVGLFIRLKLDETPAFREMKAKNACAQAPLVEIFRDHRRPFFTAVGLKIAEISYASIAGVFVISYATVQ
LGMPRSTILNGVWLSSFIALFTIPLFGWMSDKLGRKTMFFLSCLFCAGFAFPMFSLLETRDPAIVTATIVVAISCGQMVM
FGIGAPWYSELFTARLRYSGASLGFQIGAALSGGLTPFIAAALMQWSGGGTWPISLYLIGCALITAFATFMAPETARKEM
I

Sequences:

>Translated_481_residues
MNNTGEPSPERLRASAPDARLPGRRSRTGNDPACASQRPSREAFMTYTGTLAPPSAAVERNEMRRIVWSSVIGTAVEWYD
FLIYAAATALVFNKLFFPVGDPALATIAAFGTYAVGFLARPLGAAIFGHYGDKLGRKAMLATTIVIMGLGTFCIGLLPTY
EQIGIAAPILLLILRFLQGIGIGGEWGGAVLMVVENAPAKRRGLFGSMVQVGNPIGNLAAIGIFALVSQLPDSAFLSWGW
RLPFLLSIVLIAVGLFIRLKLDETPAFREMKAKNACAQAPLVEIFRDHRRPFFTAVGLKIAEISYASIAGVFVISYATVQ
LGMPRSTILNGVWLSSFIALFTIPLFGWMSDKLGRKTMFFLSCLFCAGFAFPMFSLLETRDPAIVTATIVVAISCGQMVM
FGIGAPWYSELFTARLRYSGASLGFQIGAALSGGLTPFIAAALMQWSGGGTWPISLYLIGCALITAFATFMAPETARKEM
I
>Mature_481_residues
MNNTGEPSPERLRASAPDARLPGRRSRTGNDPACASQRPSREAFMTYTGTLAPPSAAVERNEMRRIVWSSVIGTAVEWYD
FLIYAAATALVFNKLFFPVGDPALATIAAFGTYAVGFLARPLGAAIFGHYGDKLGRKAMLATTIVIMGLGTFCIGLLPTY
EQIGIAAPILLLILRFLQGIGIGGEWGGAVLMVVENAPAKRRGLFGSMVQVGNPIGNLAAIGIFALVSQLPDSAFLSWGW
RLPFLLSIVLIAVGLFIRLKLDETPAFREMKAKNACAQAPLVEIFRDHRRPFFTAVGLKIAEISYASIAGVFVISYATVQ
LGMPRSTILNGVWLSSFIALFTIPLFGWMSDKLGRKTMFFLSCLFCAGFAFPMFSLLETRDPAIVTATIVVAISCGQMVM
FGIGAPWYSELFTARLRYSGASLGFQIGAALSGGLTPFIAAALMQWSGGGTWPISLYLIGCALITAFATFMAPETARKEM
I

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=429, Percent_Identity=40.5594405594406, Blast_Score=325, Evalue=5e-90,
Organism=Escherichia coli, GI1789941, Length=426, Percent_Identity=38.0281690140845, Blast_Score=254, Evalue=6e-69,
Organism=Escherichia coli, GI1790550, Length=439, Percent_Identity=34.624145785877, Blast_Score=231, Evalue=6e-62,
Organism=Escherichia coli, GI1788942, Length=398, Percent_Identity=30.9045226130653, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI87082404, Length=411, Percent_Identity=24.5742092457421, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082231, Length=234, Percent_Identity=25.6410256410256, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 51772; Mature: 51772

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS00359 RIBOSOMAL_L11 ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNTGEPSPERLRASAPDARLPGRRSRTGNDPACASQRPSREAFMTYTGTLAPPSAAVER
CCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHH
NEMRRIVWSSVIGTAVEWYDFLIYAAATALVFNKLFFPVGDPALATIAAFGTYAVGFLAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
PLGAAIFGHYGDKLGRKAMLATTIVIMGLGTFCIGLLPTYEQIGIAAPILLLILRFLQGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
GIGGEWGGAVLMVVENAPAKRRGLFGSMVQVGNPIGNLAAIGIFALVSQLPDSAFLSWGW
CCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHCCC
RLPFLLSIVLIAVGLFIRLKLDETPAFREMKAKNACAQAPLVEIFRDHRRPFFTAVGLKI
CHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHCCCH
AEISYASIAGVFVISYATVQLGMPRSTILNGVWLSSFIALFTIPLFGWMSDKLGRKTMFF
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
LSCLFCAGFAFPMFSLLETRDPAIVTATIVVAISCGQMVMFGIGAPWYSELFTARLRYSG
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHCC
ASLGFQIGAALSGGLTPFIAAALMQWSGGGTWPISLYLIGCALITAFATFMAPETARKEM
CCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCC
I
C
>Mature Secondary Structure
MNNTGEPSPERLRASAPDARLPGRRSRTGNDPACASQRPSREAFMTYTGTLAPPSAAVER
CCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHH
NEMRRIVWSSVIGTAVEWYDFLIYAAATALVFNKLFFPVGDPALATIAAFGTYAVGFLAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
PLGAAIFGHYGDKLGRKAMLATTIVIMGLGTFCIGLLPTYEQIGIAAPILLLILRFLQGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
GIGGEWGGAVLMVVENAPAKRRGLFGSMVQVGNPIGNLAAIGIFALVSQLPDSAFLSWGW
CCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHCCC
RLPFLLSIVLIAVGLFIRLKLDETPAFREMKAKNACAQAPLVEIFRDHRRPFFTAVGLKI
CHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHCCCH
AEISYASIAGVFVISYATVQLGMPRSTILNGVWLSSFIALFTIPLFGWMSDKLGRKTMFF
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
LSCLFCAGFAFPMFSLLETRDPAIVTATIVVAISCGQMVMFGIGAPWYSELFTARLRYSG
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHCC
ASLGFQIGAALSGGLTPFIAAALMQWSGGGTWPISLYLIGCALITAFATFMAPETARKEM
CCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCC
I
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]