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The map label for this gene is lacI [H]

Identifier: 158424402

GI number: 158424402

Start: 3158820

End: 3159911

Strand: Direct

Name: lacI [H]

Synonym: AZC_2778

Alternate gene names: 158424402

Gene position: 3158820-3159911 (Clockwise)

Preceding gene: 158424398

Following gene: 158424403

Centisome position: 58.83

GC content: 68.5

Gene sequence:

>1092_bases
TTGGCAGCGCTGCCATTTTTGTTGCGGCTGTGCCTGCGACGTGGGATGGTGCCGCCCGTTGGCCGTATGACCTGCCGGAG
TGCCCCTGTGACCGCGTTTCCCGTCCGCGCCCCCGCCGCCACCCTTGCGGACGTCGCCTCGGCGGCGGGCGTGGGCGAAA
GCACGGTGTCGCGCGTGCTCCGCAACCACGGCTCCTTTTCCGAGAAGACCCGCAAGCGTGTGATGGAGGCCGCCGCCCAG
CTCGGCTATGTGCCCAACCGGATCGCGGGCACGCTGGCCTCCTCCGGGTCGCGTCTGGTTGGCATCATCGTGCCCTCGCT
GTCCAACATCGTCTTTCCGGAGGTGTTGAGCGGCGTGAACCAGGCGCTTGAGGACGAGGGCTATCAGGCGGTGTTCGCGG
TCAGCGAATACAAGATGGCCCGCGAGGAAGAGATGGTCGCCTCCATGCTCGCCTGGCGGCCCGCCGCGCTGATGGTGGCG
GGCCTCGAACACACGCCCGCGGCGCGCGCGCTTCTGGAGGCCGGGGGCACACGCATCATCGAACTGATGGATCTCGACGG
CGAGCCCATCGACATTGCGGTGGGCTTCTCCAATCTGGAGGTCGGCCGCGTCAGTGCCCGGCACCTGCTGGCGCGGGGCT
ATCGCCGGATCGCCTATGTGGGCCACGATCTTGCCGCCGACACACGCGCCGCCAAGCGCCTCGAGGGCTTCGAGGGCGTG
CTGCGGGACCAGGGCCTCGCCCTCATGGAGCGTTCGACCTTGCCCACGCTGTCCTCCATCGAGGCCGGGCGCGCGGGACT
GGAGCAGGCACTCGCCCGTCGGCCGGACCTTGAAGCAGTCTATTTCTCCAATGACGACATGGCCTTCGGGGGCTATTGCC
TGTGTCTGTCACGCGGTATCCGAGTGCCGGACCAACTGGCACTGTTCGGCTACAACGGCCTCGGCATCGGTCAGGCGCTG
CCCCAGCCACTCTCGACCATCCGCACGCCGCGGGTCGAAATCGGCGCCACAGCAGCCCGCCTCGCCGCTCGCAACGCCCC
GGCCCAGCGCATCGATCTCGGCTTCGAACTCATTGCGGGCGCTACCGCCTGA

Upstream 100 bases:

>100_bases
TCACCGTCAACTGCAACCGACATGGGTTTCCTCGCCCTTATGTTTTGGCAGCGCTGCCATTTCGATAAATACTCGATCCT
TCGCCCGTCGCAACCAGATT

Downstream 100 bases:

>100_bases
GGTTCACATCCAGCCACGGGAGACGGCCATGAGCACCGCCGACGAAAATGCCCTGCGGGACGACATCTGCCGCTTCGCGC
GTTCACTGTTCGAGCGCGGG

Product: transcriptional regulator

Products: NA

Alternate protein names: Gluconate utilization system GNT-I transcriptional repressor [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MAALPFLLRLCLRRGMVPPVGRMTCRSAPVTAFPVRAPAATLADVASAAGVGESTVSRVLRNHGSFSEKTRKRVMEAAAQ
LGYVPNRIAGTLASSGSRLVGIIVPSLSNIVFPEVLSGVNQALEDEGYQAVFAVSEYKMAREEEMVASMLAWRPAALMVA
GLEHTPAARALLEAGGTRIIELMDLDGEPIDIAVGFSNLEVGRVSARHLLARGYRRIAYVGHDLAADTRAAKRLEGFEGV
LRDQGLALMERSTLPTLSSIEAGRAGLEQALARRPDLEAVYFSNDDMAFGGYCLCLSRGIRVPDQLALFGYNGLGIGQAL
PQPLSTIRTPRVEIGATAARLAARNAPAQRIDLGFELIAGATA

Sequences:

>Translated_363_residues
MAALPFLLRLCLRRGMVPPVGRMTCRSAPVTAFPVRAPAATLADVASAAGVGESTVSRVLRNHGSFSEKTRKRVMEAAAQ
LGYVPNRIAGTLASSGSRLVGIIVPSLSNIVFPEVLSGVNQALEDEGYQAVFAVSEYKMAREEEMVASMLAWRPAALMVA
GLEHTPAARALLEAGGTRIIELMDLDGEPIDIAVGFSNLEVGRVSARHLLARGYRRIAYVGHDLAADTRAAKRLEGFEGV
LRDQGLALMERSTLPTLSSIEAGRAGLEQALARRPDLEAVYFSNDDMAFGGYCLCLSRGIRVPDQLALFGYNGLGIGQAL
PQPLSTIRTPRVEIGATAARLAARNAPAQRIDLGFELIAGATA
>Mature_362_residues
AALPFLLRLCLRRGMVPPVGRMTCRSAPVTAFPVRAPAATLADVASAAGVGESTVSRVLRNHGSFSEKTRKRVMEAAAQL
GYVPNRIAGTLASSGSRLVGIIVPSLSNIVFPEVLSGVNQALEDEGYQAVFAVSEYKMAREEEMVASMLAWRPAALMVAG
LEHTPAARALLEAGGTRIIELMDLDGEPIDIAVGFSNLEVGRVSARHLLARGYRRIAYVGHDLAADTRAAKRLEGFEGVL
RDQGLALMERSTLPTLSSIEAGRAGLEQALARRPDLEAVYFSNDDMAFGGYCLCLSRGIRVPDQLALFGYNGLGIGQALP
QPLSTIRTPRVEIGATAARLAARNAPAQRIDLGFELIAGATA

Specific function: Negative regulator for the gluconate utilization system GNT-I, the gntUKR operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI48994940, Length=330, Percent_Identity=37.8787878787879, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1790715, Length=334, Percent_Identity=30.8383233532934, Blast_Score=185, Evalue=3e-48,
Organism=Escherichia coli, GI1789068, Length=307, Percent_Identity=27.0358306188925, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1790369, Length=321, Percent_Identity=25.5451713395639, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1790194, Length=302, Percent_Identity=27.1523178807947, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787948, Length=326, Percent_Identity=26.6871165644172, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1788474, Length=310, Percent_Identity=28.7096774193548, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789202, Length=302, Percent_Identity=27.4834437086093, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786540, Length=303, Percent_Identity=29.3729372937294, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1787906, Length=271, Percent_Identity=30.2583025830258, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787580, Length=315, Percent_Identity=24.4444444444444, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790689, Length=251, Percent_Identity=24.7011952191235, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38595; Mature: 38464

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALPFLLRLCLRRGMVPPVGRMTCRSAPVTAFPVRAPAATLADVASAAGVGESTVSRVL
CCHHHHHHHHHHHCCCCCCCCCHHCCCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHH
RNHGSFSEKTRKRVMEAAAQLGYVPNRIAGTLASSGSRLVGIIVPSLSNIVFPEVLSGVN
HCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHH
QALEDEGYQAVFAVSEYKMAREEEMVASMLAWRPAALMVAGLEHTPAARALLEAGGTRII
HHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHCCCCEEE
ELMDLDGEPIDIAVGFSNLEVGRVSARHLLARGYRRIAYVGHDLAADTRAAKRLEGFEGV
EEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LRDQGLALMERSTLPTLSSIEAGRAGLEQALARRPDLEAVYFSNDDMAFGGYCLCLSRGI
HHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHCCC
RVPDQLALFGYNGLGIGQALPQPLSTIRTPRVEIGATAARLAARNAPAQRIDLGFELIAG
CCCCCEEEECCCCCCCCHHCCHHHHHHCCCCEECCHHHHHHHHCCCCHHHHHCCHHHHCC
ATA
CCC
>Mature Secondary Structure 
AALPFLLRLCLRRGMVPPVGRMTCRSAPVTAFPVRAPAATLADVASAAGVGESTVSRVL
CHHHHHHHHHHHCCCCCCCCCHHCCCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHH
RNHGSFSEKTRKRVMEAAAQLGYVPNRIAGTLASSGSRLVGIIVPSLSNIVFPEVLSGVN
HCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHH
QALEDEGYQAVFAVSEYKMAREEEMVASMLAWRPAALMVAGLEHTPAARALLEAGGTRII
HHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHCCCCEEE
ELMDLDGEPIDIAVGFSNLEVGRVSARHLLARGYRRIAYVGHDLAADTRAAKRLEGFEGV
EEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LRDQGLALMERSTLPTLSSIEAGRAGLEQALARRPDLEAVYFSNDDMAFGGYCLCLSRGI
HHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHCCC
RVPDQLALFGYNGLGIGQALPQPLSTIRTPRVEIGATAARLAARNAPAQRIDLGFELIAG
CCCCCEEEECCCCCCCCHHCCHHHHHHCCCCEECCHHHHHHHHCCCCHHHHHCCHHHHCC
ATA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]