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The map label for this gene is ygbJ [C]

Identifier: 158424401

GI number: 158424401

Start: 3157807

End: 3158712

Strand: Reverse

Name: ygbJ [C]

Synonym: AZC_2777

Alternate gene names: 158424401

Gene position: 3158712-3157807 (Counterclockwise)

Preceding gene: 158424404

Following gene: 158424400

Centisome position: 58.82

GC content: 68.1

Gene sequence:

>906_bases
ATGCGGACGGTCGCGGTCATCGGCCTCGGGGCCATGGGCTTGGGCATGGCGAAGTCGCTGCTCGCCGCAGGTCTTGTGGT
GCGCGGCTGCGATCTGTCGGCGGCCGCGGTTGCCGCGCTGGCGGAGGCCGGCGGCACCGGCTGCGCCACGCCGGCGGAAG
CGGTGCAAGGCGCCAGCGTGGTGGTGAGCGTGGTGGTGAACGCCGACCAGACAGAGGCCATCCTGTTCGGTCCTCAAGGC
ATTGCCGAGGTCATGCCCGCCAATGCGGTGTTCGTCTCTTGCGCCACCATGGCGCCGGAGAAAGCCCGCGATTTCGCGGG
CCGCCTCGAAGCGCTCGGGCGCCTCTATCTCGATGCGCCCATCTCCGGCGGGGCGGCGCGGGCGGCCAAGGGCGAACTCA
CCATTCTGGCCTCCGGCCGTCCGGAGGCCTTCGCGGCCGCTGACGCGGCGCTCGAGGCCATGTCCGCCAAGCTCTATCGG
CTCGGCGATGCGGCGGGCACCGGCGCCGCCTTCAAGATGATCAACCAGCTTCTGGCCGGGGTGCACATCGCGGCCGCCTG
TGAGGCGATCACTTTTGCCGCCCGGCAGGATCTCGACCTTGAGATGGTGTATGAAGTCATTACAGCCTCGGCGGGTAACA
GCTGGATGTTCGAGAACCGCATCCCGCATGTGCTCGCCGGCGATTATCGGCCCCTGAGTGCGGTCGAGATCTTCGTGAAG
GACCTCGGCATCATTCAGGACATGGCTCGGGCCGCCCGTTACCCCGTGCCGCTCGCGGCCGCCGCGCTCCAGCTTTATCT
CGGGGCAGCGGGTGCCGGGATGGGCCGGGATGACGATTCTTCGCTCGCACGTCTTTATGCCGGCCTGTCCGGGGTGACAT
TGCCAAAGGGGGAGAGCGTTTCATGA

Upstream 100 bases:

>100_bases
TTGCGACGGGCGAAGGATCGAGTATTTATCGAAATGGCAGCGCTGCCAAAACATAAGGGCGAGGAAACCCATGTCGGTTG
CAGTTGACGGTGAGACCCAG

Downstream 100 bases:

>100_bases
TCCGTTTCGCTGCGAACCTCCATTCGCTCTTTACGGAATTGCCGTTTCTCGACCGCTTCGCGCCCGCCGCCGAGGCCGGC
TTTGCCGCCGTGGAATTCAC

Product: oxidoreductase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MRTVAVIGLGAMGLGMAKSLLAAGLVVRGCDLSAAAVAALAEAGGTGCATPAEAVQGASVVVSVVVNADQTEAILFGPQG
IAEVMPANAVFVSCATMAPEKARDFAGRLEALGRLYLDAPISGGAARAAKGELTILASGRPEAFAAADAALEAMSAKLYR
LGDAAGTGAAFKMINQLLAGVHIAAACEAITFAARQDLDLEMVYEVITASAGNSWMFENRIPHVLAGDYRPLSAVEIFVK
DLGIIQDMARAARYPVPLAAAALQLYLGAAGAGMGRDDDSSLARLYAGLSGVTLPKGESVS

Sequences:

>Translated_301_residues
MRTVAVIGLGAMGLGMAKSLLAAGLVVRGCDLSAAAVAALAEAGGTGCATPAEAVQGASVVVSVVVNADQTEAILFGPQG
IAEVMPANAVFVSCATMAPEKARDFAGRLEALGRLYLDAPISGGAARAAKGELTILASGRPEAFAAADAALEAMSAKLYR
LGDAAGTGAAFKMINQLLAGVHIAAACEAITFAARQDLDLEMVYEVITASAGNSWMFENRIPHVLAGDYRPLSAVEIFVK
DLGIIQDMARAARYPVPLAAAALQLYLGAAGAGMGRDDDSSLARLYAGLSGVTLPKGESVS
>Mature_301_residues
MRTVAVIGLGAMGLGMAKSLLAAGLVVRGCDLSAAAVAALAEAGGTGCATPAEAVQGASVVVSVVVNADQTEAILFGPQG
IAEVMPANAVFVSCATMAPEKARDFAGRLEALGRLYLDAPISGGAARAAKGELTILASGRPEAFAAADAALEAMSAKLYR
LGDAAGTGAAFKMINQLLAGVHIAAACEAITFAARQDLDLEMVYEVITASAGNSWMFENRIPHVLAGDYRPLSAVEIFVK
DLGIIQDMARAARYPVPLAAAALQLYLGAAGAGMGRDDDSSLARLYAGLSGVTLPKGESVS

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=289, Percent_Identity=28.3737024221453, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI40556376, Length=285, Percent_Identity=23.859649122807, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1789092, Length=298, Percent_Identity=46.6442953020134, Blast_Score=242, Evalue=2e-65,
Organism=Escherichia coli, GI1790315, Length=294, Percent_Identity=32.312925170068, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI145693186, Length=293, Percent_Identity=28.3276450511945, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1786719, Length=278, Percent_Identity=26.2589928057554, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17557316, Length=274, Percent_Identity=26.6423357664234, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24655230, Length=291, Percent_Identity=28.8659793814433, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI19922568, Length=291, Percent_Identity=28.8659793814433, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI28574115, Length=282, Percent_Identity=23.758865248227, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30441; Mature: 30441

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTVAVIGLGAMGLGMAKSLLAAGLVVRGCDLSAAAVAALAEAGGTGCATPAEAVQGASV
CCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCHHE
VVSVVVNADQTEAILFGPQGIAEVMPANAVFVSCATMAPEKARDFAGRLEALGRLYLDAP
EEEEEECCCCCCEEEECCCCHHHHCCCCHHEEEHHHCCCHHHHHHHHHHHHHHHHEEECC
ISGGAARAAKGELTILASGRPEAFAAADAALEAMSAKLYRLGDAAGTGAAFKMINQLLAG
CCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VHIAAACEAITFAARQDLDLEMVYEVITASAGNSWMFENRIPHVLAGDYRPLSAVEIFVK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCCEECCCCCCHHHHHHHHH
DLGIIQDMARAARYPVPLAAAALQLYLGAAGAGMGRDDDSSLARLYAGLSGVTLPKGESV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCC
S
C
>Mature Secondary Structure
MRTVAVIGLGAMGLGMAKSLLAAGLVVRGCDLSAAAVAALAEAGGTGCATPAEAVQGASV
CCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCHHE
VVSVVVNADQTEAILFGPQGIAEVMPANAVFVSCATMAPEKARDFAGRLEALGRLYLDAP
EEEEEECCCCCCEEEECCCCHHHHCCCCHHEEEHHHCCCHHHHHHHHHHHHHHHHEEECC
ISGGAARAAKGELTILASGRPEAFAAADAALEAMSAKLYRLGDAAGTGAAFKMINQLLAG
CCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VHIAAACEAITFAARQDLDLEMVYEVITASAGNSWMFENRIPHVLAGDYRPLSAVEIFVK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCCEECCCCCCHHHHHHHHH
DLGIIQDMARAARYPVPLAAAALQLYLGAAGAGMGRDDDSSLARLYAGLSGVTLPKGESV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]