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The map label for this gene is glgX [H]

Identifier: 158424374

GI number: 158424374

Start: 3123741

End: 3125879

Strand: Reverse

Name: glgX [H]

Synonym: AZC_2750

Alternate gene names: 158424374

Gene position: 3125879-3123741 (Counterclockwise)

Preceding gene: 158424375

Following gene: 158424373

Centisome position: 58.21

GC content: 66.25

Gene sequence:

>2139_bases
TTGCGGGCGCGGCAGCGTTTCAGTTCGGATGTCGAACCGCGCGAGCCCCCCATGCAGCATGATTTTCACCCCGTCCGCGA
AATCCTGGCCGGCGATCCGACGCGGCTCGGCGCCGTCTACGACGGGCAGGGGACGAATTTCGCGCTGTTTTCCGCGCATG
CGGAGCGGGTGGAACTCTGCCTCTTCGACGGCACCGGCCGGCAGGAGCTGGAGCGCATCACCCTGCCCGAATATACGGAC
GAGGTCTGGCACGGCTATCTGCCGGGCGTCGGACCCGGCACGCTCTATGGCTACCGGGTCCACGGCCCCTATGCCCCGGA
GGAGGGGCACCGCTTCAATCCCAACAAGCTGCTGATTGATCCCTACGCCCGCGATCTCGTGGGGGCGGTGAAATCGTCTC
CCGCTCATTATGGCTATGTGCTCGGCGGCGAGGAGCAGGATCTCGCCTTCGACACGCAGGACAGCGCGCCCTTCATGCCC
AAGTGCCGCGTGGTGGATCCGCGCGAGGACGGCGGTCGCATCCACGCCAAGCCCCGCGTGCCGTGGGACCGGACGGTGAT
CTATGAGACCCATGTGAAGGGCATGACGAAGCTGCATCCTTCGGTGCCGGCGGACCTGCGCGGGACCTTCGAGGGCATGG
GGCATCGCGCGGTGGTGGACTATGTGAAGAGCCTCGGCGTCACTGCCGTCGAGCTTCTGCCCGTGCATGCCTTTCCCGAT
GATCCCTATCTCGTGGAAAAGGGCCTCGCCAATTACTGGGGCTACAACACGATTTCCTTCTTCACGCCGGAGCTGCGCTA
TCGCGGCCCCCGCGGGCGCGCCGGTTTTCGCCGCATGGTGCGGGCCTTCCATGATGCGGGCATTGAGGTGATCCTCGACG
TGGTGTTCAACCACACCGCCGAGGGCAATGAGCTGGGGCCGACGCTTTCCTTCAAGGGCATCGACAACTTCAGCTACTAC
CGGACGATGCCCGACACGCCGCGCCACTACATCAACGACACCGGCACCGGCAACACCATGAACGTCGCCCATCCGCGCGT
GATGCGGATGGTGCTGGACAGTCTGCGCTACTGGGCGAGCGAGATGGAGGTGGACGGCTTCCGCTTCGACCTCGCCACGA
TCCTCGGCCGCGAGCCCCATGGCTTTGATCCGCGCGGCGGCTTCCTCGATGCCATCGGGCAGGATCCGGTGCTCGCGCAG
GTGAAGCTGATCGCGGAGCCCTGGGACATCGGCCCTGGCGGCTATCAGGTCGGCGCCTTCCCGCCCGGCTGGGGCGAGTG
GAACGACAAATTCCGCGACACCATGCGCGATTACTGGCGCGATACCGAGGGCACCACAGGCGACTTTGCGGCTCGCCTCA
CGGGCTCCGGCGACATCTATAATCAGCGCGGGCGCCGGCCCTGGGCTTCGGTGAATTTCATCACCGCCCACGACGGTTTC
ACCCTCAACGACGTCGTTTCCTACAACGACAAGCACAACGAGGCGAACGGCGAGAACAATGCCGACGGCCACTCCCACAA
CCGCAGCTTCAATTACGGCGTGGAAGGCCCGACCGACGATCCGGGCATTCGCGCCGTGCGCGAGCGGCAGAAGCGCAACC
TGCTGGCGACGCTGCTCCTCTCGCACGGCACGCCCATGCTGCTCGGCGGCGACGAGTTCGGCCGCACGCAGGATGGCAAC
AACAATTGTTATTGCCAGGACAATCCTCTCTCCTGGATCGACTGGAAGGGCATAACGGAGGAGGGCAAGGCGCTGACCGC
CTTCGTGCGCAAGCTGCTGGCCCTGCGCGCCCGCCAGCCCTTGCTGCGGCGGGCGAGTTTCCGCGATGGCATGGTCGTCA
CCTGGCTCAATCCGGGCGGCGGCGAGCAGACGCTGGAACAATGGGCCGATGCCGGCGCCACCTCCATCGCCGTTCGCCTG
GCGCGGGAGGACCTTCAGGGACAGGAGGGTGTTTGGCGTGAACTCATCCTCGCCTTCAATCCCCATGAGGCGCCCGTGCC
GTTCGTCCTGCCCGAACGCGGGGGGAAGCGCTGGGAGGCGGTGCTGGACACGGACATGGGCGAGGGGCCGGCCCGCTCAA
CGGCCGGTGGCGCGGAGGCCGTCGAACTCGCGGGTCGCTCTCTGGTGCTGTTCGCGTGA

Upstream 100 bases:

>100_bases
TCCCGCCGCTTCGAGGAGACCAAGGCGCTGAAGAAGCGCGTGGCGGAACTGGAAAAGGCGGCGGGGGCCGCCTGAACCCT
CGCCTCCGGCAAGGGAAACT

Downstream 100 bases:

>100_bases
GGGCGACGGGAGCGGCCCCCTAGGCAAGCGGCCTCCCAGCGCCTAACCATGGCCCCGGAACGTTCGATCCGGGGACCCCA
TGACCGCCTATTCCGCGCTT

Product: glycogen debranching protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 712; Mature: 712

Protein sequence:

>712_residues
MRARQRFSSDVEPREPPMQHDFHPVREILAGDPTRLGAVYDGQGTNFALFSAHAERVELCLFDGTGRQELERITLPEYTD
EVWHGYLPGVGPGTLYGYRVHGPYAPEEGHRFNPNKLLIDPYARDLVGAVKSSPAHYGYVLGGEEQDLAFDTQDSAPFMP
KCRVVDPREDGGRIHAKPRVPWDRTVIYETHVKGMTKLHPSVPADLRGTFEGMGHRAVVDYVKSLGVTAVELLPVHAFPD
DPYLVEKGLANYWGYNTISFFTPELRYRGPRGRAGFRRMVRAFHDAGIEVILDVVFNHTAEGNELGPTLSFKGIDNFSYY
RTMPDTPRHYINDTGTGNTMNVAHPRVMRMVLDSLRYWASEMEVDGFRFDLATILGREPHGFDPRGGFLDAIGQDPVLAQ
VKLIAEPWDIGPGGYQVGAFPPGWGEWNDKFRDTMRDYWRDTEGTTGDFAARLTGSGDIYNQRGRRPWASVNFITAHDGF
TLNDVVSYNDKHNEANGENNADGHSHNRSFNYGVEGPTDDPGIRAVRERQKRNLLATLLLSHGTPMLLGGDEFGRTQDGN
NNCYCQDNPLSWIDWKGITEEGKALTAFVRKLLALRARQPLLRRASFRDGMVVTWLNPGGGEQTLEQWADAGATSIAVRL
AREDLQGQEGVWRELILAFNPHEAPVPFVLPERGGKRWEAVLDTDMGEGPARSTAGGAEAVELAGRSLVLFA

Sequences:

>Translated_712_residues
MRARQRFSSDVEPREPPMQHDFHPVREILAGDPTRLGAVYDGQGTNFALFSAHAERVELCLFDGTGRQELERITLPEYTD
EVWHGYLPGVGPGTLYGYRVHGPYAPEEGHRFNPNKLLIDPYARDLVGAVKSSPAHYGYVLGGEEQDLAFDTQDSAPFMP
KCRVVDPREDGGRIHAKPRVPWDRTVIYETHVKGMTKLHPSVPADLRGTFEGMGHRAVVDYVKSLGVTAVELLPVHAFPD
DPYLVEKGLANYWGYNTISFFTPELRYRGPRGRAGFRRMVRAFHDAGIEVILDVVFNHTAEGNELGPTLSFKGIDNFSYY
RTMPDTPRHYINDTGTGNTMNVAHPRVMRMVLDSLRYWASEMEVDGFRFDLATILGREPHGFDPRGGFLDAIGQDPVLAQ
VKLIAEPWDIGPGGYQVGAFPPGWGEWNDKFRDTMRDYWRDTEGTTGDFAARLTGSGDIYNQRGRRPWASVNFITAHDGF
TLNDVVSYNDKHNEANGENNADGHSHNRSFNYGVEGPTDDPGIRAVRERQKRNLLATLLLSHGTPMLLGGDEFGRTQDGN
NNCYCQDNPLSWIDWKGITEEGKALTAFVRKLLALRARQPLLRRASFRDGMVVTWLNPGGGEQTLEQWADAGATSIAVRL
AREDLQGQEGVWRELILAFNPHEAPVPFVLPERGGKRWEAVLDTDMGEGPARSTAGGAEAVELAGRSLVLFA
>Mature_712_residues
MRARQRFSSDVEPREPPMQHDFHPVREILAGDPTRLGAVYDGQGTNFALFSAHAERVELCLFDGTGRQELERITLPEYTD
EVWHGYLPGVGPGTLYGYRVHGPYAPEEGHRFNPNKLLIDPYARDLVGAVKSSPAHYGYVLGGEEQDLAFDTQDSAPFMP
KCRVVDPREDGGRIHAKPRVPWDRTVIYETHVKGMTKLHPSVPADLRGTFEGMGHRAVVDYVKSLGVTAVELLPVHAFPD
DPYLVEKGLANYWGYNTISFFTPELRYRGPRGRAGFRRMVRAFHDAGIEVILDVVFNHTAEGNELGPTLSFKGIDNFSYY
RTMPDTPRHYINDTGTGNTMNVAHPRVMRMVLDSLRYWASEMEVDGFRFDLATILGREPHGFDPRGGFLDAIGQDPVLAQ
VKLIAEPWDIGPGGYQVGAFPPGWGEWNDKFRDTMRDYWRDTEGTTGDFAARLTGSGDIYNQRGRRPWASVNFITAHDGF
TLNDVVSYNDKHNEANGENNADGHSHNRSFNYGVEGPTDDPGIRAVRERQKRNLLATLLLSHGTPMLLGGDEFGRTQDGN
NNCYCQDNPLSWIDWKGITEEGKALTAFVRKLLALRARQPLLRRASFRDGMVVTWLNPGGGEQTLEQWADAGATSIAVRL
AREDLQGQEGVWRELILAFNPHEAPVPFVLPERGGKRWEAVLDTDMGEGPARSTAGGAEAVELAGRSLVLFA

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=217, Percent_Identity=27.6497695852535, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI2367229, Length=622, Percent_Identity=49.1961414790997, Blast_Score=568, Evalue=1e-163,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 79451; Mature: 79451

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRARQRFSSDVEPREPPMQHDFHPVREILAGDPTRLGAVYDGQGTNFALFSAHAERVELC
CCCHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEECCCCCCEEEEECCCCEEEEE
LFDGTGRQELERITLPEYTDEVWHGYLPGVGPGTLYGYRVHGPYAPEEGHRFNPNKLLID
EECCCCHHHHHHCCCCCHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEC
PYARDLVGAVKSSPAHYGYVLGGEEQDLAFDTQDSAPFMPKCRVVDPREDGGRIHAKPRV
HHHHHHHHHHHCCCCCEEEEECCCCCCCEECCCCCCCCCCCCEEECCCCCCCEEEECCCC
PWDRTVIYETHVKGMTKLHPSVPADLRGTFEGMGHRAVVDYVKSLGVTAVELLPVHAFPD
CCCCEEEEEECCCCHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEEEECCCC
DPYLVEKGLANYWGYNTISFFTPELRYRGPRGRAGFRRMVRAFHDAGIEVILDVVFNHTA
CCHHHHHHHHHHCCCCEEEEECCHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCC
EGNELGPTLSFKGIDNFSYYRTMPDTPRHYINDTGTGNTMNVAHPRVMRMVLDSLRYWAS
CCCCCCCEEEECCCCCCCEEECCCCCCHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHH
EMEVDGFRFDLATILGREPHGFDPRGGFLDAIGQDPVLAQVKLIAEPWDIGPGGYQVGAF
HCCCCCEEEHHHHHHCCCCCCCCCCCCHHHCCCCCCCEEEEEEEECCCCCCCCCEECCCC
PPGWGEWNDKFRDTMRDYWRDTEGTTGDFAARLTGSGDIYNQRGRRPWASVNFITAHDGF
CCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCC
TLNDVVSYNDKHNEANGENNADGHSHNRSFNYGVEGPTDDPGIRAVRERQKRNLLATLLL
CHHHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SHGTPMLLGGDEFGRTQDGNNNCYCQDNPLSWIDWKGITEEGKALTAFVRKLLALRARQP
HCCCCEEECCHHCCCCCCCCCCEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHH
LLRRASFRDGMVVTWLNPGGGEQTLEQWADAGATSIAVRLAREDLQGQEGVWRELILAFN
HHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHEEEC
PHEAPVPFVLPERGGKRWEAVLDTDMGEGPARSTAGGAEAVELAGRSLVLFA
CCCCCCCEEECCCCCCEEEHEECCCCCCCCCCCCCCCHHHHHHCCCEEEEEC
>Mature Secondary Structure
MRARQRFSSDVEPREPPMQHDFHPVREILAGDPTRLGAVYDGQGTNFALFSAHAERVELC
CCCHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEECCCCCCEEEEECCCCEEEEE
LFDGTGRQELERITLPEYTDEVWHGYLPGVGPGTLYGYRVHGPYAPEEGHRFNPNKLLID
EECCCCHHHHHHCCCCCHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEC
PYARDLVGAVKSSPAHYGYVLGGEEQDLAFDTQDSAPFMPKCRVVDPREDGGRIHAKPRV
HHHHHHHHHHHCCCCCEEEEECCCCCCCEECCCCCCCCCCCCEEECCCCCCCEEEECCCC
PWDRTVIYETHVKGMTKLHPSVPADLRGTFEGMGHRAVVDYVKSLGVTAVELLPVHAFPD
CCCCEEEEEECCCCHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEEEECCCC
DPYLVEKGLANYWGYNTISFFTPELRYRGPRGRAGFRRMVRAFHDAGIEVILDVVFNHTA
CCHHHHHHHHHHCCCCEEEEECCHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCC
EGNELGPTLSFKGIDNFSYYRTMPDTPRHYINDTGTGNTMNVAHPRVMRMVLDSLRYWAS
CCCCCCCEEEECCCCCCCEEECCCCCCHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHH
EMEVDGFRFDLATILGREPHGFDPRGGFLDAIGQDPVLAQVKLIAEPWDIGPGGYQVGAF
HCCCCCEEEHHHHHHCCCCCCCCCCCCHHHCCCCCCCEEEEEEEECCCCCCCCCEECCCC
PPGWGEWNDKFRDTMRDYWRDTEGTTGDFAARLTGSGDIYNQRGRRPWASVNFITAHDGF
CCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCC
TLNDVVSYNDKHNEANGENNADGHSHNRSFNYGVEGPTDDPGIRAVRERQKRNLLATLLL
CHHHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SHGTPMLLGGDEFGRTQDGNNNCYCQDNPLSWIDWKGITEEGKALTAFVRKLLALRARQP
HCCCCEEECCHHCCCCCCCCCCEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHH
LLRRASFRDGMVVTWLNPGGGEQTLEQWADAGATSIAVRLAREDLQGQEGVWRELILAFN
HHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHEEEC
PHEAPVPFVLPERGGKRWEAVLDTDMGEGPARSTAGGAEAVELAGRSLVLFA
CCCCCCCEEECCCCCCEEEHEECCCCCCCCCCCCCCCHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]