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The map label for this gene is cueO [H]

Identifier: 158424369

GI number: 158424369

Start: 3117166

End: 3118638

Strand: Direct

Name: cueO [H]

Synonym: AZC_2745

Alternate gene names: 158424369

Gene position: 3117166-3118638 (Clockwise)

Preceding gene: 158424368

Following gene: 158424370

Centisome position: 58.05

GC content: 66.94

Gene sequence:

>1473_bases
GTGAAGATTTCGCGTCGCAGTCTGTTGCTGGGAGGGGCCGCCGTCGGCGTGGTGTCCTTCGTGCCGTCTGCCATCGGTCA
GGTGCCTGCCGCCTTCGACACGCTGCCCGCCTCCGCCCCGACCCGCAATCTGACGCTGACCGCGCGCGAGATAGACGCCC
GGCTGCTCGGTGCCGATGGCCCCCTGACACGTCGCGTCTGCGCCTATGACGGCGAACTGTTCAAGATGTTCCGCTTCCAG
CGGGGCACGCGCCTCATCGTCGATTTCGAGAACGCCCTTCCCGATCCCACCACGGTCCACTGGCACGGCATCCGCCTGCC
CTATCTCTCCGACGGCGTGCCGCCGCTGACGCAGGACGTGGTCGAGCCCGGCGGGCACTTCCGCTACAACGTGCCGCTAC
CGGACGCCGGCTTCTATTTCATCCATCCCCATTGCGACGAGACCGGTCAGGTCGGGCGCGGCCTTGCGGCCCTGCTGGTT
GTGGATGATCCCGCCGAGGCAAAGGCGGGCTATGATGCCGAATGCTTCGTGGTGGTGAAGGACTGGCGCCTGGCCCCCGA
CGGCAAGTGGCTGGACATGACGACGGACGACGGCGCCGCGAACGCCGGCACCTTCGGCACCATCCGCGCCACCAACGGCA
AGACGACGCCCCCCCGCGTTGAGGCGCCGGCCTTCGGTGACGTCCGCGTGCGCGCGCTGGTGGCGGATGCCACGCGGGTC
ATCGACCTCGGCTGCGACAGCGATGATGCCTTCCTGATTGCCGTGGACGGGCAGGCCCTGCCGCCGGTCTCCCTCAATGC
GCTGCCGAACGGCGTATGGCGCATGGGGTCGGCCATGCGCGCCGACGTGCAGATCCGGATGCCCGCGCCGGGCGAGGAAG
TGAAGATCTACGATTATCGCTCGGACGAGCCTTACCTGCTGACGACGCTGGTCGCTGTGGATACGGGCAAGCGCAAGACG
CCGGTGAAGCCTCGGCCGCTGCCCGCGCCACAGGTGCCGCTGCCGGACCTGAAGAAGGCGCAGAAGCTGGAATTCCTGCT
CCAGGTGTCCGCCGGCCCCACCGCCGTCTCACCGCCTTTGCCGGCGGACGATCCCCTGTCCAAGGCCCTCGCCAATTCGC
TATGCGTGGGATCGACAACCCACTGGGCCATCAGCCGCGACAGCTGGTCCACCCGCGAGGATCTGCGGCTGCCGCCGCCT
CTGGCCCGCATGGATCATGGCGCCAGCTACATCGTCTCCATGACCAACGTGACCAAGCACGTGCATCCCATGCACCTGCA
CGGCCACATCTTCCGGGTGCTCTCCCACTCCAAGAAGCCGAAGATGCCGCAATACCTTGCGGACACGGTGATCGTGGAGC
CGAACGAAACAGTGGAGATTGCCTTCAAGGCGGTGAGCGGCAACTGGGTGTTCCACTGCCACATCCTGGAACACATGGAC
ACCGGCCTCATGGGCTGGTTCAAGGTCGCCTGA

Upstream 100 bases:

>100_bases
CCTCTGCCTGCTGGCACCCTTCGGTGCCCGCCGCCCGGACGGCCCGACGATCGGGGCCACAGCGGATCCTGCTGAGCGGG
GTTCGACGGGAGATGTTGAA

Downstream 100 bases:

>100_bases
TGATCTCCCGTCCGACCCGCGTCCTGCTCGGCCTGAGCCTCCTTGCCGGCCTGTGCGGGGCGGCAACCGCTGCCCTCGCC
GCCGGAGGCCTCGACGGGCT

Product: metallo-oxidoreductase

Products: NA

Alternate protein names: Copper efflux oxidase [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MKISRRSLLLGGAAVGVVSFVPSAIGQVPAAFDTLPASAPTRNLTLTAREIDARLLGADGPLTRRVCAYDGELFKMFRFQ
RGTRLIVDFENALPDPTTVHWHGIRLPYLSDGVPPLTQDVVEPGGHFRYNVPLPDAGFYFIHPHCDETGQVGRGLAALLV
VDDPAEAKAGYDAECFVVVKDWRLAPDGKWLDMTTDDGAANAGTFGTIRATNGKTTPPRVEAPAFGDVRVRALVADATRV
IDLGCDSDDAFLIAVDGQALPPVSLNALPNGVWRMGSAMRADVQIRMPAPGEEVKIYDYRSDEPYLLTTLVAVDTGKRKT
PVKPRPLPAPQVPLPDLKKAQKLEFLLQVSAGPTAVSPPLPADDPLSKALANSLCVGSTTHWAISRDSWSTREDLRLPPP
LARMDHGASYIVSMTNVTKHVHPMHLHGHIFRVLSHSKKPKMPQYLADTVIVEPNETVEIAFKAVSGNWVFHCHILEHMD
TGLMGWFKVA

Sequences:

>Translated_490_residues
MKISRRSLLLGGAAVGVVSFVPSAIGQVPAAFDTLPASAPTRNLTLTAREIDARLLGADGPLTRRVCAYDGELFKMFRFQ
RGTRLIVDFENALPDPTTVHWHGIRLPYLSDGVPPLTQDVVEPGGHFRYNVPLPDAGFYFIHPHCDETGQVGRGLAALLV
VDDPAEAKAGYDAECFVVVKDWRLAPDGKWLDMTTDDGAANAGTFGTIRATNGKTTPPRVEAPAFGDVRVRALVADATRV
IDLGCDSDDAFLIAVDGQALPPVSLNALPNGVWRMGSAMRADVQIRMPAPGEEVKIYDYRSDEPYLLTTLVAVDTGKRKT
PVKPRPLPAPQVPLPDLKKAQKLEFLLQVSAGPTAVSPPLPADDPLSKALANSLCVGSTTHWAISRDSWSTREDLRLPPP
LARMDHGASYIVSMTNVTKHVHPMHLHGHIFRVLSHSKKPKMPQYLADTVIVEPNETVEIAFKAVSGNWVFHCHILEHMD
TGLMGWFKVA
>Mature_490_residues
MKISRRSLLLGGAAVGVVSFVPSAIGQVPAAFDTLPASAPTRNLTLTAREIDARLLGADGPLTRRVCAYDGELFKMFRFQ
RGTRLIVDFENALPDPTTVHWHGIRLPYLSDGVPPLTQDVVEPGGHFRYNVPLPDAGFYFIHPHCDETGQVGRGLAALLV
VDDPAEAKAGYDAECFVVVKDWRLAPDGKWLDMTTDDGAANAGTFGTIRATNGKTTPPRVEAPAFGDVRVRALVADATRV
IDLGCDSDDAFLIAVDGQALPPVSLNALPNGVWRMGSAMRADVQIRMPAPGEEVKIYDYRSDEPYLLTTLVAVDTGKRKT
PVKPRPLPAPQVPLPDLKKAQKLEFLLQVSAGPTAVSPPLPADDPLSKALANSLCVGSTTHWAISRDSWSTREDLRLPPP
LARMDHGASYIVSMTNVTKHVHPMHLHGHIFRVLSHSKKPKMPQYLADTVIVEPNETVEIAFKAVSGNWVFHCHILEHMD
TGLMGWFKVA

Specific function: Probably involved in periplasmic detoxification of copper by oxidizing Cu(+) to Cu(2+) and thus preventing its uptake into the cytoplasm. Possesses phenoloxidase and ferroxidase activities and might be involved in the production of polyphenolic compounds

COG id: COG2132

COG function: function code Q; Putative multicopper oxidases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 plastocyanin-like domains [H]

Homologues:

Organism=Escherichia coli, GI1786314, Length=544, Percent_Identity=29.5955882352941, Blast_Score=118, Evalue=7e-28,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001117
- InterPro:   IPR011706
- InterPro:   IPR011707
- InterPro:   IPR002355
- InterPro:   IPR008972
- InterPro:   IPR006311 [H]

Pfam domain/function: PF00394 Cu-oxidase; PF07731 Cu-oxidase_2; PF07732 Cu-oxidase_3 [H]

EC number: NA

Molecular weight: Translated: 53321; Mature: 53321

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00037 MYB_1 ; PS00079 MULTICOPPER_OXIDASE1 ; PS00080 MULTICOPPER_OXIDASE2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISRRSLLLGGAAVGVVSFVPSAIGQVPAAFDTLPASAPTRNLTLTAREIDARLLGADG
CCCCCCEEEECHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCEEEEEEEECCEEEECCCC
PLTRRVCAYDGELFKMFRFQRGTRLIVDFENALPDPTTVHWHGIRLPYLSDGVPPLTQDV
CHHHHHHHCCCHHHHHHHHCCCCEEEEEEHHCCCCCCEEEEEEEECCCCCCCCCCHHHHH
VEPGGHFRYNVPLPDAGFYFIHPHCDETGQVGRGLAALLVVDDPAEAKAGYDAECFVVVK
HCCCCCEEEECCCCCCCEEEEECCCCCCCCCCCCEEEEEEEECCHHHCCCCCCEEEEEEE
DWRLAPDGKWLDMTTDDGAANAGTFGTIRATNGKTTPPRVEAPAFGDVRVRALVADATRV
CCEECCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEEECCEEE
IDLGCDSDDAFLIAVDGQALPPVSLNALPNGVWRMGSAMRADVQIRMPAPGEEVKIYDYR
EEECCCCCCEEEEEECCCCCCCEECCCCCCHHHHCCCCEEEEEEEECCCCCCEEEEEEEC
SDEPYLLTTLVAVDTGKRKTPVKPRPLPAPQVPLPDLKKAQKLEFLLQVSAGPTAVSPPL
CCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHEEEEEEECCCCCCCCCCC
PADDPLSKALANSLCVGSTTHWAISRDSWSTREDLRLPPPLARMDHGASYIVSMTNVTKH
CCCCHHHHHHHHHEECCCCCEEEEECCCCCCCCCCCCCCCHHHHCCCCEEEEEECHHHHH
VHPMHLHGHIFRVLSHSKKPKMPQYLADTVIVEPNETVEIAFKAVSGNWVFHCHILEHMD
CCEEEHHHHHHHHHHCCCCCCCCHHHHCEEEECCCCEEEEEEEEECCCEEEEEEEEHHCC
TGLMGWFKVA
CCCCEEEEEC
>Mature Secondary Structure
MKISRRSLLLGGAAVGVVSFVPSAIGQVPAAFDTLPASAPTRNLTLTAREIDARLLGADG
CCCCCCEEEECHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCEEEEEEEECCEEEECCCC
PLTRRVCAYDGELFKMFRFQRGTRLIVDFENALPDPTTVHWHGIRLPYLSDGVPPLTQDV
CHHHHHHHCCCHHHHHHHHCCCCEEEEEEHHCCCCCCEEEEEEEECCCCCCCCCCHHHHH
VEPGGHFRYNVPLPDAGFYFIHPHCDETGQVGRGLAALLVVDDPAEAKAGYDAECFVVVK
HCCCCCEEEECCCCCCCEEEEECCCCCCCCCCCCEEEEEEEECCHHHCCCCCCEEEEEEE
DWRLAPDGKWLDMTTDDGAANAGTFGTIRATNGKTTPPRVEAPAFGDVRVRALVADATRV
CCEECCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEEECCEEE
IDLGCDSDDAFLIAVDGQALPPVSLNALPNGVWRMGSAMRADVQIRMPAPGEEVKIYDYR
EEECCCCCCEEEEEECCCCCCCEECCCCCCHHHHCCCCEEEEEEEECCCCCCEEEEEEEC
SDEPYLLTTLVAVDTGKRKTPVKPRPLPAPQVPLPDLKKAQKLEFLLQVSAGPTAVSPPL
CCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHEEEEEEECCCCCCCCCCC
PADDPLSKALANSLCVGSTTHWAISRDSWSTREDLRLPPPLARMDHGASYIVSMTNVTKH
CCCCHHHHHHHHHEECCCCCEEEEECCCCCCCCCCCCCCCHHHHCCCCEEEEEECHHHHH
VHPMHLHGHIFRVLSHSKKPKMPQYLADTVIVEPNETVEIAFKAVSGNWVFHCHILEHMD
CCEEEHHHHHHHHHHCCCCCCCCHHHHCEEEECCCCEEEEEEEEECCCEEEEEEEEHHCC
TGLMGWFKVA
CCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]