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The map label for this gene is gatA [H]

Identifier: 158424317

GI number: 158424317

Start: 3052608

End: 3053999

Strand: Reverse

Name: gatA [H]

Synonym: AZC_2693

Alternate gene names: 158424317

Gene position: 3053999-3052608 (Counterclockwise)

Preceding gene: 158424318

Following gene: 158424314

Centisome position: 56.87

GC content: 68.89

Gene sequence:

>1392_bases
ATGTCGTCCCCTCTGGACCTTTCCGCCCTCGAACTCGTCGCTGCCTTCCGCGCCCGCAGGCTTTCGCCCAGCGAATATTT
CGACGCCTTGCTGCCCTATGTGGCGGCGAGCGAGCCCAAGCTCGGCGCGCTCTATGCCTTCGAGCCCGAGCGGGTGCGCG
CGGAAGCTGCCGCCTCAACGGCGCGCTGGGCGAAGAGCGCGCCCAACGGGCCGCTCGATGGCGTGCCTGTCACGCTGAAG
GAACTCATCGCGACGGAAGGGCTGCCGGTGCCGCTCGGCACCGCCGCCACCGATCTCGTGCCCGCCACCGCCGATGCGCC
GCCCGCCGCGCGCATGAAGGAAGCGGGGGCGATCCTCCTCGCCAAGACCACCGTGCCGGATTACGGCATGCTCTCCTCCG
GCCTCTCCAGCTTCCACACGCTGGCCCGCAATCCCTGGAACCTCGCCTGCAACCCGGGCGGCTCCAGCGCCGGGGCTGCG
GCGGCAGGGGCGGCGGGCTATGGGCCGCTGCATGTTGGCACGGACATTGGCGGTTCCGTGCGCCTGCCGGCCGGCTGGTG
TGGCCTAGTCGGCTTCAAGCCGAGCCTGGGGCGCATTCCCATCGACCCCTATTATGTCGGCCGCTGCGCCGGCCCCATGA
CGCGCACGGTGGCCGAGAGCGCGCTGATGATGCAGTTCCTCGCCAAGCCCGACTGGCGCGATGCCACGAGCCTGCCACCC
GAGGACATCGACTGGATGGACCTTGAGGGCATGAGCGTGAAAGGCCTGCGCATTGGCCTCATGCTGGAGGCCGGCTGCGG
CATGGACGTGGAGGACGAGGTGCGCAAGGCCGTGGAAGCGGCAGCGCGGCGCTTCGAGGCGGAGGGCGCGGAGATCGTGC
CCGCGCCGGGCGTCCTCACCCGCGCGATGCTGGATGGGCTCGATGATTTCTGGCGCGCCCGCTCGTGGGATGACATCAGT
GCGCTGCCGCCGGAGAAGCGGGCCAAGGTGCTGCCCTATATCGTGGACTGGGCGGCGGGCGGCGCCCATGTGGACGGCCC
GCGCGCGGTGCGGGGCTTCAACCAGACCATGAAGATGCGCCAGGCCTGCGCCACGCTCTTCCACGGCCTCGATTATCTGC
TAACGCCCACCAACAATGTCTCGGCCTTCCCCGCCGAATGGGCTTCCCCCGTCAATGATCCGGCCAAGCCCTTCGAGCAC
ATCTGCTTCACGGTGCCCTGGAACATGTCCGAGCAGCCGGCCATCTCCATCAACTGCGGCTTCACCCAAGCGGGCATGCC
CATCGGCCTCCAGATTGTCGGACCGCGTTTTGCGGACCACGCGGTGCTGCGCATAGCCCATCTTTATGAGGGCTGGCGCG
GGCCGATCACCAACTGGCCAAAGCTCGCCTGA

Upstream 100 bases:

>100_bases
ATCGCGGATGGGCGCGCCAGCGCCTGCCATCACGCCGAAGACCTTTCCCCTTCTCTTGTCCCCTAAGCGGCCGTCCGGCG
CGCCTCAGGAGCGTTGTTGC

Downstream 100 bases:

>100_bases
ACGGGCTCTCAGGCGGGCTGGAGCAAAGGCTCCAGCGCCGCCGCGATGCGCGGATGATGCGTGGGCGCAAGCGGGAGGAT
GGGGCGGGGCGGCAGCGCCG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MSSPLDLSALELVAAFRARRLSPSEYFDALLPYVAASEPKLGALYAFEPERVRAEAAASTARWAKSAPNGPLDGVPVTLK
ELIATEGLPVPLGTAATDLVPATADAPPAARMKEAGAILLAKTTVPDYGMLSSGLSSFHTLARNPWNLACNPGGSSAGAA
AAGAAGYGPLHVGTDIGGSVRLPAGWCGLVGFKPSLGRIPIDPYYVGRCAGPMTRTVAESALMMQFLAKPDWRDATSLPP
EDIDWMDLEGMSVKGLRIGLMLEAGCGMDVEDEVRKAVEAAARRFEAEGAEIVPAPGVLTRAMLDGLDDFWRARSWDDIS
ALPPEKRAKVLPYIVDWAAGGAHVDGPRAVRGFNQTMKMRQACATLFHGLDYLLTPTNNVSAFPAEWASPVNDPAKPFEH
ICFTVPWNMSEQPAISINCGFTQAGMPIGLQIVGPRFADHAVLRIAHLYEGWRGPITNWPKLA

Sequences:

>Translated_463_residues
MSSPLDLSALELVAAFRARRLSPSEYFDALLPYVAASEPKLGALYAFEPERVRAEAAASTARWAKSAPNGPLDGVPVTLK
ELIATEGLPVPLGTAATDLVPATADAPPAARMKEAGAILLAKTTVPDYGMLSSGLSSFHTLARNPWNLACNPGGSSAGAA
AAGAAGYGPLHVGTDIGGSVRLPAGWCGLVGFKPSLGRIPIDPYYVGRCAGPMTRTVAESALMMQFLAKPDWRDATSLPP
EDIDWMDLEGMSVKGLRIGLMLEAGCGMDVEDEVRKAVEAAARRFEAEGAEIVPAPGVLTRAMLDGLDDFWRARSWDDIS
ALPPEKRAKVLPYIVDWAAGGAHVDGPRAVRGFNQTMKMRQACATLFHGLDYLLTPTNNVSAFPAEWASPVNDPAKPFEH
ICFTVPWNMSEQPAISINCGFTQAGMPIGLQIVGPRFADHAVLRIAHLYEGWRGPITNWPKLA
>Mature_462_residues
SSPLDLSALELVAAFRARRLSPSEYFDALLPYVAASEPKLGALYAFEPERVRAEAAASTARWAKSAPNGPLDGVPVTLKE
LIATEGLPVPLGTAATDLVPATADAPPAARMKEAGAILLAKTTVPDYGMLSSGLSSFHTLARNPWNLACNPGGSSAGAAA
AGAAGYGPLHVGTDIGGSVRLPAGWCGLVGFKPSLGRIPIDPYYVGRCAGPMTRTVAESALMMQFLAKPDWRDATSLPPE
DIDWMDLEGMSVKGLRIGLMLEAGCGMDVEDEVRKAVEAAARRFEAEGAEIVPAPGVLTRAMLDGLDDFWRARSWDDISA
LPPEKRAKVLPYIVDWAAGGAHVDGPRAVRGFNQTMKMRQACATLFHGLDYLLTPTNNVSAFPAEWASPVNDPAKPFEHI
CFTVPWNMSEQPAISINCGFTQAGMPIGLQIVGPRFADHAVLRIAHLYEGWRGPITNWPKLA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=498, Percent_Identity=26.3052208835341, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI195972892, Length=268, Percent_Identity=29.8507462686567, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI166795287, Length=253, Percent_Identity=32.0158102766798, Blast_Score=69, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17537465, Length=496, Percent_Identity=26.2096774193548, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17543272, Length=430, Percent_Identity=24.8837209302326, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17556264, Length=258, Percent_Identity=28.2945736434109, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17538252, Length=448, Percent_Identity=26.3392857142857, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=194, Percent_Identity=33.5051546391753, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=248, Percent_Identity=28.6290322580645, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=248, Percent_Identity=28.6290322580645, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=448, Percent_Identity=24.5535714285714, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320448, Length=182, Percent_Identity=32.4175824175824, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323950, Length=209, Percent_Identity=26.3157894736842, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648113, Length=495, Percent_Identity=25.8585858585859, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21356731, Length=481, Percent_Identity=25.1559251559252, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI161078093, Length=506, Percent_Identity=24.5059288537549, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24644968, Length=502, Percent_Identity=24.3027888446215, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI45550774, Length=500, Percent_Identity=24.8, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648435, Length=500, Percent_Identity=24.8, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648437, Length=500, Percent_Identity=24.8, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648441, Length=424, Percent_Identity=25.7075471698113, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24648439, Length=424, Percent_Identity=25.7075471698113, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24652985, Length=328, Percent_Identity=25, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922090, Length=328, Percent_Identity=25, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24652981, Length=328, Percent_Identity=25, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24652983, Length=328, Percent_Identity=25, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 49296; Mature: 49165

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPLDLSALELVAAFRARRLSPSEYFDALLPYVAASEPKLGALYAFEPERVRAEAAAST
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHH
ARWAKSAPNGPLDGVPVTLKELIATEGLPVPLGTAATDLVPATADAPPAARMKEAGAILL
HHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHCCEEEE
AKTTVPDYGMLSSGLSSFHTLARNPWNLACNPGGSSAGAAAAGAAGYGPLHVGTDIGGSV
EECCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHCCCCCCCCEEECCCCCCCE
RLPAGWCGLVGFKPSLGRIPIDPYYVGRCAGPMTRTVAESALMMQFLAKPDWRDATSLPP
ECCCCCEEEECCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
EDIDWMDLEGMSVKGLRIGLMLEAGCGMDVEDEVRKAVEAAARRFEAEGAEIVPAPGVLT
CCCCCEECCCCEECCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCHHHH
RAMLDGLDDFWRARSWDDISALPPEKRAKVLPYIVDWAAGGAHVDGPRAVRGFNQTMKMR
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHH
QACATLFHGLDYLLTPTNNVSAFPAEWASPVNDPAKPFEHICFTVPWNMSEQPAISINCG
HHHHHHHHCCCEEECCCCCCCCCCHHHCCCCCCCHHHHHHEEEECCCCCCCCCEEEEECC
FTQAGMPIGLQIVGPRFADHAVLRIAHLYEGWRGPITNWPKLA
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SSPLDLSALELVAAFRARRLSPSEYFDALLPYVAASEPKLGALYAFEPERVRAEAAAST
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHH
ARWAKSAPNGPLDGVPVTLKELIATEGLPVPLGTAATDLVPATADAPPAARMKEAGAILL
HHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHCCEEEE
AKTTVPDYGMLSSGLSSFHTLARNPWNLACNPGGSSAGAAAAGAAGYGPLHVGTDIGGSV
EECCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHCCCCCCCCEEECCCCCCCE
RLPAGWCGLVGFKPSLGRIPIDPYYVGRCAGPMTRTVAESALMMQFLAKPDWRDATSLPP
ECCCCCEEEECCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
EDIDWMDLEGMSVKGLRIGLMLEAGCGMDVEDEVRKAVEAAARRFEAEGAEIVPAPGVLT
CCCCCEECCCCEECCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCHHHH
RAMLDGLDDFWRARSWDDISALPPEKRAKVLPYIVDWAAGGAHVDGPRAVRGFNQTMKMR
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHH
QACATLFHGLDYLLTPTNNVSAFPAEWASPVNDPAKPFEHICFTVPWNMSEQPAISINCG
HHHHHHHHCCCEEECCCCCCCCCCHHHCCCCCCCHHHHHHEEEECCCCCCCCCEEEEECC
FTQAGMPIGLQIVGPRFADHAVLRIAHLYEGWRGPITNWPKLA
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA