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The map label for this gene is ilvD [H]

Identifier: 158424267

GI number: 158424267

Start: 2993781

End: 2995511

Strand: Reverse

Name: ilvD [H]

Synonym: AZC_2643

Alternate gene names: 158424267

Gene position: 2995511-2993781 (Counterclockwise)

Preceding gene: 158424268

Following gene: 158424266

Centisome position: 55.78

GC content: 66.55

Gene sequence:

>1731_bases
ATGACCAAGACACCCGAGCAACTGCGCAGCGCACGCTATTTCGAGCCCGACGACTTCCGCTCCTTCGGCCATCGCTCGCG
CATGAGCCAGATGGGCTATGCGGCGGAGGAGTATCGCGGCAAGCCGGTCATCGCGATCATCAACACCTGGTCCGATCTCA
ACCAGTGCCACGCCCATTTCAAGCAGCGCGTGGAGGATGTGAAGCGGGGCATTCTTCAGGCGGGCGGCTTTCCGGTGGAA
CTGCCCGCCATCTCGCTCTCCGAGAGCAAGGTGAAGCCCACCACCATGCTCTACCGCAACTTCCTCGCCATGGAGACGGA
GGAGCTCATCCGTTCCCATCCCGTGGACGGAGCGGTGCTGATGGGCGGTTGCGACAAGACCACGCCCGGCCTGCTGCTGG
GCGCCACCAGCGCGGGCGTTCCGGCCATCTATGTGCCGGCCGGACCCATGCTGCGCGGCAACTGGCACGGCAAGGTGCTG
GGCTCCGGCTCGGACGCCTTCAAATATTGGGACGAGCGCCGCGCCGGCAACATCTCAGACAAGGACTGGGCCGAGATGGA
GGTAGGCATCGCCCGCTCCTATGGCGTGTGCATGACCATGGGCACGGCGGCTACCATGACGGCGCTCGCCGATGTGATCG
GCATGACACTGCCCGGCGCCTCCTCCATCCCGGCGGCGGATGCCAACCACATCCGCATGTGCGCGGCCGCCGGCCGGCGG
ATCGTCGAGATGGTGTGGGAGGATCTCACCCCGGACAAGATCCAGACCGAGGGCGCGTTCCGCAATGCCATCAACGTCGC
CATGGCCATGGGATGCTCGACAAACGCGGTGATCCACATCATCGCCATGGCCCGCCGCGCCGGCCACGCCATCGGGCTCG
AGGACTTCGACGCGGCGAGCCGCAAGGTGCCGGTGATCGCCAACATCCGCCCGAGCGGCGAGCGCTATCTGATGGAGGAT
TTCTATTACGCCGGCGGCCTCCTCGGCCTGCTGACCCGGCTCACGCCCCATCTCGACCTGTCGCTCATCAACGTCTCCGG
GCAGACCTGGGCGCAGTCGCTGGAGGGAGCAGAGGTCTACAATGACGATGTGATCCGCCCGCTGGACAATCCCATCTATG
CGGAAGGCGCGCTGGCGCTGCTGAAGGGCAATCTCGCGCCGAGCGGCTGCGTGATGAAACCCTCCGCCGCCGCTCCCCAT
CTCCTCACCCATTCCGGTCCCGCGCTGGTCTTCGACGACTATCCGGCGCTTAAGGCTAATATCGACCGGGACGATCTCGA
CGTGACGCCAGACACGGTTCTGGTGCTGCGCAATGCCGGTCCCTTGGGCGGGCCGGGCATGCCGGAATGGGGCATGCTGC
CCATCCCGAAGAAGCTGGTGAAGCAGGGGGTGCGCGACATGGTGCGCATCTCGGATGCGCGCATGAGCGGCACCTCCTAT
GGCGCCTGCATCCTGCATGTCTCGCCGGAATCCTACATCGGCGGCCCGCTGGCTCTCGTGCGCACCGGCGACATCATCAC
CGTGGACGTGCCCAACCGTAGCATCCGCCTGGAGGTGTCCGACGCCGAGCTGGAGCGCCGCCGGGTCGAGACGCCGCCCC
CCGCGCCGCGCTACGAGCGCGGCTATGGCTGGATGTTCTCCCGCCACATCGGCCAGGCCGACCAGGGCTGCGATTTCGAT
TTCCTGCGCACCGACTTCGGCGCGCCGGTGCCCGAGCCCGCCATCTTCTGA

Upstream 100 bases:

>100_bases
TCGTGGTCGAGAACGTGATCTTCCGCACCATCGAGCGCCGCACGGTCCTGAAATGGGGCCTCCAGCACTGACCCGCGCCT
CCCACGACCGAGCCCCTTCC

Downstream 100 bases:

>100_bases
CTTCCCGACAATCCGGAGTTTGCCCGATGCTGTCCGACGCCACCCGCGAGAAGCTGAAGCACGTCAGCACCGCGACCCTC
GCCACGGCCCTGTTCAAGCG

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 576; Mature: 575

Protein sequence:

>576_residues
MTKTPEQLRSARYFEPDDFRSFGHRSRMSQMGYAAEEYRGKPVIAIINTWSDLNQCHAHFKQRVEDVKRGILQAGGFPVE
LPAISLSESKVKPTTMLYRNFLAMETEELIRSHPVDGAVLMGGCDKTTPGLLLGATSAGVPAIYVPAGPMLRGNWHGKVL
GSGSDAFKYWDERRAGNISDKDWAEMEVGIARSYGVCMTMGTAATMTALADVIGMTLPGASSIPAADANHIRMCAAAGRR
IVEMVWEDLTPDKIQTEGAFRNAINVAMAMGCSTNAVIHIIAMARRAGHAIGLEDFDAASRKVPVIANIRPSGERYLMED
FYYAGGLLGLLTRLTPHLDLSLINVSGQTWAQSLEGAEVYNDDVIRPLDNPIYAEGALALLKGNLAPSGCVMKPSAAAPH
LLTHSGPALVFDDYPALKANIDRDDLDVTPDTVLVLRNAGPLGGPGMPEWGMLPIPKKLVKQGVRDMVRISDARMSGTSY
GACILHVSPESYIGGPLALVRTGDIITVDVPNRSIRLEVSDAELERRRVETPPPAPRYERGYGWMFSRHIGQADQGCDFD
FLRTDFGAPVPEPAIF

Sequences:

>Translated_576_residues
MTKTPEQLRSARYFEPDDFRSFGHRSRMSQMGYAAEEYRGKPVIAIINTWSDLNQCHAHFKQRVEDVKRGILQAGGFPVE
LPAISLSESKVKPTTMLYRNFLAMETEELIRSHPVDGAVLMGGCDKTTPGLLLGATSAGVPAIYVPAGPMLRGNWHGKVL
GSGSDAFKYWDERRAGNISDKDWAEMEVGIARSYGVCMTMGTAATMTALADVIGMTLPGASSIPAADANHIRMCAAAGRR
IVEMVWEDLTPDKIQTEGAFRNAINVAMAMGCSTNAVIHIIAMARRAGHAIGLEDFDAASRKVPVIANIRPSGERYLMED
FYYAGGLLGLLTRLTPHLDLSLINVSGQTWAQSLEGAEVYNDDVIRPLDNPIYAEGALALLKGNLAPSGCVMKPSAAAPH
LLTHSGPALVFDDYPALKANIDRDDLDVTPDTVLVLRNAGPLGGPGMPEWGMLPIPKKLVKQGVRDMVRISDARMSGTSY
GACILHVSPESYIGGPLALVRTGDIITVDVPNRSIRLEVSDAELERRRVETPPPAPRYERGYGWMFSRHIGQADQGCDFD
FLRTDFGAPVPEPAIF
>Mature_575_residues
TKTPEQLRSARYFEPDDFRSFGHRSRMSQMGYAAEEYRGKPVIAIINTWSDLNQCHAHFKQRVEDVKRGILQAGGFPVEL
PAISLSESKVKPTTMLYRNFLAMETEELIRSHPVDGAVLMGGCDKTTPGLLLGATSAGVPAIYVPAGPMLRGNWHGKVLG
SGSDAFKYWDERRAGNISDKDWAEMEVGIARSYGVCMTMGTAATMTALADVIGMTLPGASSIPAADANHIRMCAAAGRRI
VEMVWEDLTPDKIQTEGAFRNAINVAMAMGCSTNAVIHIIAMARRAGHAIGLEDFDAASRKVPVIANIRPSGERYLMEDF
YYAGGLLGLLTRLTPHLDLSLINVSGQTWAQSLEGAEVYNDDVIRPLDNPIYAEGALALLKGNLAPSGCVMKPSAAAPHL
LTHSGPALVFDDYPALKANIDRDDLDVTPDTVLVLRNAGPLGGPGMPEWGMLPIPKKLVKQGVRDMVRISDARMSGTSYG
ACILHVSPESYIGGPLALVRTGDIITVDVPNRSIRLEVSDAELERRRVETPPPAPRYERGYGWMFSRHIGQADQGCDFDF
LRTDFGAPVPEPAIF

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=545, Percent_Identity=32.8440366972477, Blast_Score=257, Evalue=2e-69,
Organism=Escherichia coli, GI1788157, Length=526, Percent_Identity=30.9885931558935, Blast_Score=178, Evalue=8e-46,
Organism=Escherichia coli, GI2367371, Length=461, Percent_Identity=29.9349240780911, Blast_Score=149, Evalue=7e-37,
Organism=Escherichia coli, GI1786464, Length=539, Percent_Identity=28.9424860853432, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6322476, Length=547, Percent_Identity=31.0786106032907, Blast_Score=250, Evalue=4e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 62566; Mature: 62435

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00886 ILVD_EDD_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTPEQLRSARYFEPDDFRSFGHRSRMSQMGYAAEEYRGKPVIAIINTWSDLNQCHAHF
CCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEECHHHHHHHHHHH
KQRVEDVKRGILQAGGFPVELPAISLSESKVKPTTMLYRNFLAMETEELIRSHPVDGAVL
HHHHHHHHHHHHHCCCCEEECCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEE
MGGCDKTTPGLLLGATSAGVPAIYVPAGPMLRGNWHGKVLGSGSDAFKYWDERRAGNISD
ECCCCCCCCCEEEEECCCCCCEEEECCCCEEECCCCCEEEECCCHHHHHHHHHCCCCCCC
KDWAEMEVGIARSYGVCMTMGTAATMTALADVIGMTLPGASSIPAADANHIRMCAAAGRR
CCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
IVEMVWEDLTPDKIQTEGAFRNAINVAMAMGCSTNAVIHIIAMARRAGHAIGLEDFDAAS
HHHHHHHCCCCCCEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
RKVPVIANIRPSGERYLMEDFYYAGGLLGLLTRLTPHLDLSLINVSGQTWAQSLEGAEVY
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHCCCCHHC
NDDVIRPLDNPIYAEGALALLKGNLAPSGCVMKPSAAAPHLLTHSGPALVFDDYPALKAN
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCEECCCCCCCCEEECCCCEEEEECCCCCCCC
IDRDDLDVTPDTVLVLRNAGPLGGPGMPEWGMLPIPKKLVKQGVRDMVRISDARMSGTSY
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
GACILHVSPESYIGGPLALVRTGDIITVDVPNRSIRLEVSDAELERRRVETPPPAPRYER
CEEEEEECCCCCCCCCEEEEEECCEEEEECCCCEEEEEECHHHHHHHCCCCCCCCCCCCC
GYGWMFSRHIGQADQGCDFDFLRTDFGAPVPEPAIF
CCCCHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TKTPEQLRSARYFEPDDFRSFGHRSRMSQMGYAAEEYRGKPVIAIINTWSDLNQCHAHF
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEECHHHHHHHHHHH
KQRVEDVKRGILQAGGFPVELPAISLSESKVKPTTMLYRNFLAMETEELIRSHPVDGAVL
HHHHHHHHHHHHHCCCCEEECCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEE
MGGCDKTTPGLLLGATSAGVPAIYVPAGPMLRGNWHGKVLGSGSDAFKYWDERRAGNISD
ECCCCCCCCCEEEEECCCCCCEEEECCCCEEECCCCCEEEECCCHHHHHHHHHCCCCCCC
KDWAEMEVGIARSYGVCMTMGTAATMTALADVIGMTLPGASSIPAADANHIRMCAAAGRR
CCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
IVEMVWEDLTPDKIQTEGAFRNAINVAMAMGCSTNAVIHIIAMARRAGHAIGLEDFDAAS
HHHHHHHCCCCCCEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
RKVPVIANIRPSGERYLMEDFYYAGGLLGLLTRLTPHLDLSLINVSGQTWAQSLEGAEVY
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHCCCCHHC
NDDVIRPLDNPIYAEGALALLKGNLAPSGCVMKPSAAAPHLLTHSGPALVFDDYPALKAN
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCEECCCCCCCCEEECCCCEEEEECCCCCCCC
IDRDDLDVTPDTVLVLRNAGPLGGPGMPEWGMLPIPKKLVKQGVRDMVRISDARMSGTSY
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
GACILHVSPESYIGGPLALVRTGDIITVDVPNRSIRLEVSDAELERRRVETPPPAPRYER
CEEEEEECCCCCCCCCEEEEEECCEEEEECCCCEEEEEECHHHHHHHCCCCCCCCCCCCC
GYGWMFSRHIGQADQGCDFDFLRTDFGAPVPEPAIF
CCCCHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA