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The map label for this gene is garD [H]

Identifier: 158424260

GI number: 158424260

Start: 2984845

End: 2986407

Strand: Reverse

Name: garD [H]

Synonym: AZC_2636

Alternate gene names: 158424260

Gene position: 2986407-2984845 (Counterclockwise)

Preceding gene: 158424261

Following gene: 158424246

Centisome position: 55.62

GC content: 67.31

Gene sequence:

>1563_bases
GTGCCAGACGGGAGGACACAGATGACGTACCAGGGCCCGGCCGGGGTCGAGACCCCGCGCACCATCAAGGTCCACGAGAG
CGACAATGTCGCCATTGTGGTCAACAGCTTCGGCCTCAAGCCCGGCACCGATCTGCCCGATGGCACGCGCCTCGTGGAAT
TCGTGCCTCAGGGCCACAAGGTGGCCCTGACCGATATTGCCGATGGCGCGCCCATCCTTCGCTATGGCGAGGTGATCGGG
CGTGCCATTGGCGACATCCCGCGCGGGGCGTGGGTGGAGGAATCCCGCGTCGCCATGCCCGTTCCACCGGATCTTTCGGC
GCTGGAAAAGGCCACGCGTGTTCCCGCCCCGCTGCCTCCGCTGGAAGGCTATACGTTTGAGGGCTACCGCAATCCGGACG
GATCGGTGGGCACCAAGAACATCCTCGCCATCTCCACCAGCGTGCAGTGCGTGGCCGGAACGCTGGATTTCGCCATCAGG
CGCATCCGTGAGGATCTGCTGCCGCGCTATCCCAATGTGGATGACGTGGTGGCGGTGACACACGCTTATGGCTGCGGCGT
CGCCATCGGCGCCCCGCAGTCGGTGGTGCCCATCCGCACCCTCCAGAGCCTCGCCACGCATCCGAACTTCGGTGGCGAGG
TGATGATCGTCTCGCTGGGCTGCGAGAAGCTCCAGCCCGAGCGCCTGTTGCCCGAGGGCAAGCAGGGCATCGTCCGCCTT
CAGGATGAGGCCTTCCACGGCTTCGATGCCATGATCGGCGCCATATTGGAGATGGCCGAGGCGCGGCTTGAGGTGCTGAA
CCGCCGGAAGCGGGAGACCGTGCCGGCCTCGGAACTGGTGGTGGGCCTCCAGTGCGGCGGCTCGGACGCTTTCTCCGGCG
TCACCGCCAATCCCGCGCTCGGCTATGCCGCCGACCTGCTGGTGCGAGCGGGCGCGACGGTCATGTTCTCGGAAGTCACC
GAGGTGCGCGACGCCATCCATCTGCTCACCCCGCGCGCCGCAACGCCCGAGGTGGCCGATGCGCTGATCCGCGAGATGGC
CTGGTATGACGATTATCTGGAGCAGGGCGGCGCCGACCGCAGCGCCAACCCCTCTCCCGGCAACAAGAAGGGCGGCCTCG
CCAATGTGGTGGAGAAAGCGCTTGGCTCCGTGGCCAAGTCCGGCTCCAGCACCATTGCGGGGGTGCTCGCGCCCGGCGAG
AAGGCGAAGCAGAAGGGGCTCATCTTCGCCGCGACGCCGGCCAGCGACTTCGTGTGCGGCACGCTTCAGCTGGCCTCCGG
CATGACACTTCAGGTCTTCTCCACGGGCCGGGGCACGCCCTATGGCCTTTCCGCCGCGCCGGTCATCAAGGTCGCCACGC
GCACGGACCTCGCCCGCCGCTGGCATGACCTCATCGACCTCGACGCCGGCCGCATCGCCACGGGCGAAGCCACCATCGAG
GACGTGGGCTGGGATCTGTTCCGCCTCATGCTCGATGTGGCCAGCGGCAAGGTGAAGCCCTGGTCCGACAAGTGGGGCCT
CTTCAACGCGCTGACGCTGTTTAACCCGGCGCCCATCACCTGA

Upstream 100 bases:

>100_bases
CGCGAGCAGGGACGCTACGCGGCCGAATTCTACACAACGGTGAAGACCGCCTATACCAACGCGGGCTGAGCGGCGCCGCG
CCAATAACAAGAACAGGCGC

Downstream 100 bases:

>100_bases
CGTTTCGGCGGCCGCCTGGCCGCCTCTTTTCGTTTGGTAATGCCTAATCAAGCTGGACCGGATTCTGCTGGATCCGGTCC
AGCTTGATTAGGCATTACCT

Product: galactarate dehydratase

Products: NA

Alternate protein names: GalcD [H]

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MPDGRTQMTYQGPAGVETPRTIKVHESDNVAIVVNSFGLKPGTDLPDGTRLVEFVPQGHKVALTDIADGAPILRYGEVIG
RAIGDIPRGAWVEESRVAMPVPPDLSALEKATRVPAPLPPLEGYTFEGYRNPDGSVGTKNILAISTSVQCVAGTLDFAIR
RIREDLLPRYPNVDDVVAVTHAYGCGVAIGAPQSVVPIRTLQSLATHPNFGGEVMIVSLGCEKLQPERLLPEGKQGIVRL
QDEAFHGFDAMIGAILEMAEARLEVLNRRKRETVPASELVVGLQCGGSDAFSGVTANPALGYAADLLVRAGATVMFSEVT
EVRDAIHLLTPRAATPEVADALIREMAWYDDYLEQGGADRSANPSPGNKKGGLANVVEKALGSVAKSGSSTIAGVLAPGE
KAKQKGLIFAATPASDFVCGTLQLASGMTLQVFSTGRGTPYGLSAAPVIKVATRTDLARRWHDLIDLDAGRIATGEATIE
DVGWDLFRLMLDVASGKVKPWSDKWGLFNALTLFNPAPIT

Sequences:

>Translated_520_residues
MPDGRTQMTYQGPAGVETPRTIKVHESDNVAIVVNSFGLKPGTDLPDGTRLVEFVPQGHKVALTDIADGAPILRYGEVIG
RAIGDIPRGAWVEESRVAMPVPPDLSALEKATRVPAPLPPLEGYTFEGYRNPDGSVGTKNILAISTSVQCVAGTLDFAIR
RIREDLLPRYPNVDDVVAVTHAYGCGVAIGAPQSVVPIRTLQSLATHPNFGGEVMIVSLGCEKLQPERLLPEGKQGIVRL
QDEAFHGFDAMIGAILEMAEARLEVLNRRKRETVPASELVVGLQCGGSDAFSGVTANPALGYAADLLVRAGATVMFSEVT
EVRDAIHLLTPRAATPEVADALIREMAWYDDYLEQGGADRSANPSPGNKKGGLANVVEKALGSVAKSGSSTIAGVLAPGE
KAKQKGLIFAATPASDFVCGTLQLASGMTLQVFSTGRGTPYGLSAAPVIKVATRTDLARRWHDLIDLDAGRIATGEATIE
DVGWDLFRLMLDVASGKVKPWSDKWGLFNALTLFNPAPIT
>Mature_519_residues
PDGRTQMTYQGPAGVETPRTIKVHESDNVAIVVNSFGLKPGTDLPDGTRLVEFVPQGHKVALTDIADGAPILRYGEVIGR
AIGDIPRGAWVEESRVAMPVPPDLSALEKATRVPAPLPPLEGYTFEGYRNPDGSVGTKNILAISTSVQCVAGTLDFAIRR
IREDLLPRYPNVDDVVAVTHAYGCGVAIGAPQSVVPIRTLQSLATHPNFGGEVMIVSLGCEKLQPERLLPEGKQGIVRLQ
DEAFHGFDAMIGAILEMAEARLEVLNRRKRETVPASELVVGLQCGGSDAFSGVTANPALGYAADLLVRAGATVMFSEVTE
VRDAIHLLTPRAATPEVADALIREMAWYDDYLEQGGADRSANPSPGNKKGGLANVVEKALGSVAKSGSSTIAGVLAPGEK
AKQKGLIFAATPASDFVCGTLQLASGMTLQVFSTGRGTPYGLSAAPVIKVATRTDLARRWHDLIDLDAGRIATGEATIED
VGWDLFRLMLDVASGKVKPWSDKWGLFNALTLFNPAPIT

Specific function: D-galactarate metabolism; first step. [C]

COG id: COG2721

COG function: function code G; Altronate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uxaA family [H]

Homologues:

Organism=Escherichia coli, GI1789516, Length=515, Percent_Identity=67.1844660194175, Blast_Score=715, Evalue=0.0,
Organism=Escherichia coli, GI1789475, Length=517, Percent_Identity=33.6557059961315, Blast_Score=226, Evalue=3e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007392
- InterPro:   IPR017654
- InterPro:   IPR013974 [H]

Pfam domain/function: PF04295 GD_AH_C; PF08666 SAF [H]

EC number: =4.2.1.42 [H]

Molecular weight: Translated: 55161; Mature: 55029

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDGRTQMTYQGPAGVETPRTIKVHESDNVAIVVNSFGLKPGTDLPDGTRLVEFVPQGHK
CCCCCCEEEECCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCHHHHCCCCCCE
VALTDIADGAPILRYGEVIGRAIGDIPRGAWVEESRVAMPVPPDLSALEKATRVPAPLPP
EEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCHHHHHHHHCCCCCCCC
LEGYTFEGYRNPDGSVGTKNILAISTSVQCVAGTLDFAIRRIREDLLPRYPNVDDVVAVT
CCCEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
HAYGCGVAIGAPQSVVPIRTLQSLATHPNFGGEVMIVSLGCEKLQPERLLPEGKQGIVRL
HHCCCCEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHCCCCCCCCEEEE
QDEAFHGFDAMIGAILEMAEARLEVLNRRKRETVPASELVVGLQCGGSDAFSGVTANPAL
EHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEECCCCCCCCCCCCCCH
GYAADLLVRAGATVMFSEVTEVRDAIHLLTPRAATPEVADALIREMAWYDDYLEQGGADR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
SANPSPGNKKGGLANVVEKALGSVAKSGSSTIAGVLAPGEKAKQKGLIFAATPASDFVCG
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCEEEEECCCCCCEEE
TLQLASGMTLQVFSTGRGTPYGLSAAPVIKVATRTDLARRWHDLIDLDAGRIATGEATIE
HHHHCCCCEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCEECCCCHHH
DVGWDLFRLMLDVASGKVKPWSDKWGLFNALTLFNPAPIT
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
PDGRTQMTYQGPAGVETPRTIKVHESDNVAIVVNSFGLKPGTDLPDGTRLVEFVPQGHK
CCCCCEEEECCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCHHHHCCCCCCE
VALTDIADGAPILRYGEVIGRAIGDIPRGAWVEESRVAMPVPPDLSALEKATRVPAPLPP
EEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCHHHHHHHHCCCCCCCC
LEGYTFEGYRNPDGSVGTKNILAISTSVQCVAGTLDFAIRRIREDLLPRYPNVDDVVAVT
CCCEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
HAYGCGVAIGAPQSVVPIRTLQSLATHPNFGGEVMIVSLGCEKLQPERLLPEGKQGIVRL
HHCCCCEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHCCCCCCCCEEEE
QDEAFHGFDAMIGAILEMAEARLEVLNRRKRETVPASELVVGLQCGGSDAFSGVTANPAL
EHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEECCCCCCCCCCCCCCH
GYAADLLVRAGATVMFSEVTEVRDAIHLLTPRAATPEVADALIREMAWYDDYLEQGGADR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
SANPSPGNKKGGLANVVEKALGSVAKSGSSTIAGVLAPGEKAKQKGLIFAATPASDFVCG
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCEEEEECCCCCCEEE
TLQLASGMTLQVFSTGRGTPYGLSAAPVIKVATRTDLARRWHDLIDLDAGRIATGEATIE
HHHHCCCCEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCEECCCCHHH
DVGWDLFRLMLDVASGKVKPWSDKWGLFNALTLFNPAPIT
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704254; 9384377 [H]