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The map label for this gene is thiC [H]

Identifier: 158424231

GI number: 158424231

Start: 2942174

End: 2944030

Strand: Direct

Name: thiC [H]

Synonym: AZC_2607

Alternate gene names: 158424231

Gene position: 2942174-2944030 (Clockwise)

Preceding gene: 158424230

Following gene: 158424238

Centisome position: 54.79

GC content: 66.02

Gene sequence:

>1857_bases
TTGAAGGAGATCCGTATGCTCGACGAAGCCTCCACCTCCGCCCTCAAGGTGACGACGGGGCCCCTTCCCCATTCCCGCAA
GGTCTTCATCCCGGCCGAGAATCCGGCTGTGAAAGTCGCCATGCGCGAGATCGGCCTGTCCGATCCAAGCGAGCGGCCGG
TGCGCGTCTATGACACCTCCGGCCCCTATACGGACCCCAACGCCATCATCGATCTCACCAAGGGCCTGCCCGAACTGCGC
CGCCAGTGGGTGCTTGATCGCGGCGACGTCGAAGAGGTGGAAGGTCGTGCCCAGAAGCCGGAGGACGATGGCCTGAAGCC
CGGCCAGGTGAGCCACCTGCCCGCCTTCGATCGCACGGGCCGCAAGCCGCTGCGCGCCAAAGCCGGCAAACGTCCGACCC
AGATGGCTTACGCCAAGGCCGGCATCGTGACGCCGGAAATGGAATATGTGGCGGCGCGCGAGAATCTCGCCCGCGCCAAT
GGCGGCGCCCACCCCAAGCACCAGGCCATGGGCCTCGGCGCGGCGATTCCCGCCGACATCACCGGCGAGTTCGTGCGGCA
GGAAGTGGCGCGCGGGCGGGCCATCATCCCGTCCAACATCAACCATCTCGAGACCGAGCCCATGGCCATCGGTCGCAACT
TCCTCGTGAAGATCAACGCCAACATCGGCAATTCGGCCGTGACGTCAGGCGTGGCGGAAGAGGTGGACAAGCTGGTGTGG
GCCATCCGCTGGGGTGGCGACACGGTGATGGACCTCTCCACCGGCAAGAACATCCACACCATCCGCGAATGGATCATCCG
CAACGCGCCGGTGCCGATCGGCACGGTGCCGATCTATCAGGCGCTGGAGAAGGTCAACGGCGTGGTGGAGGACCTCAACT
GGGAGATCTTCCGCGACACGGTGATCGAGCAGGCCGAGCAGGGCGTGGACTACATCACCGTCCATGCGGGCGTGCGCCTC
CCCTATATCCCGCTCACCGCCAACCGCGTCACGGGCATCGTCTCACGCGGCGGCTCGATCATGGCCAAGTGGTGCCTGGC
CCACCACAAGGAGAGCTTCCTCTACGAACATTTCGAGGAGCTGTGCGAGATCCTCGCCCAGTATGACGTCGCCTTCTCGC
TGGGCGATGGCCTGCGCCCCGGCTCCATCGCGGACGCCAATGACGCCGCCCAGTTCGCCGAGCTGGAGACGCTGGGTGAA
CTCACCGAGATCGCCTGGAAGCACGACGTGCAGGTGATGATCGAGGGGCCGGGCCACGTGCCCATGCACCTCATCCGCGA
GAATGTGGAGAAGCAGCTCAAGCTCTGCCACGAGGCGCCATTCTATACGCTTGGCCCGCTCGTGACGGACATCTCCCCCG
GCTATGACCACATCTCCTCGGCCATCGGCGCGGCGATGATCGGCTGGTTCGGCACCGCCATGCTCTGCTACGTGACGCCG
AAGGAGCATCTCGGCCTTCCGGATCGCGACGACGTGAAGGCCGGCGTGATCTCCTACAAGATCGCCGCCCATGCGGCCGA
CCTTGCCAAGGGCCATCCCGCCGCGCGCATCCGGGACGATGCACTGTCCCGTGCGCGCTTCTCCTTCCGCTGGCGCGACC
AGTTCGCGCTCTCGCTCGATCCGGAAACGGCGGAGGCCTATCACGACGAGACGCTGCCGGCGGAAGGCGCGAAGGTCGCG
CACTTCTGCTCCATGTGCGGACCGAAATTCTGCTCCATGAAGATCAGCCAGGAGCTGAAGATCGAGGCCGGCGATATCGC
CCAGAGCGGCATGGCTGAGATGGCCGAGAAGTTCCGCGCCAGCGGCGGCGAGATCTACCAGCCCGCCCCGGACGTGGCCG
CGCCGCCCTCCGCCTGA

Upstream 100 bases:

>100_bases
CGAGGTTCTCCTCCTCAGCAGGCCGGTCCCTCCCCAGAGGGTAGTCTGTCAGGCCAACCTGCTCCTGAAGCTCATCCCGG
GTCTCATTTGAAGCGCGAGC

Downstream 100 bases:

>100_bases
CAATGACGGGTCGCGCCATGCTCTGGCGCGGCCCTCTTTTTTTTCGCGGGCACACAAGGGTGTGGATGACCGGGCCGAAG
GTCGGCAGAAAGGCACCCGC

Product: thiamine biosynthesis protein

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MKEIRMLDEASTSALKVTTGPLPHSRKVFIPAENPAVKVAMREIGLSDPSERPVRVYDTSGPYTDPNAIIDLTKGLPELR
RQWVLDRGDVEEVEGRAQKPEDDGLKPGQVSHLPAFDRTGRKPLRAKAGKRPTQMAYAKAGIVTPEMEYVAARENLARAN
GGAHPKHQAMGLGAAIPADITGEFVRQEVARGRAIIPSNINHLETEPMAIGRNFLVKINANIGNSAVTSGVAEEVDKLVW
AIRWGGDTVMDLSTGKNIHTIREWIIRNAPVPIGTVPIYQALEKVNGVVEDLNWEIFRDTVIEQAEQGVDYITVHAGVRL
PYIPLTANRVTGIVSRGGSIMAKWCLAHHKESFLYEHFEELCEILAQYDVAFSLGDGLRPGSIADANDAAQFAELETLGE
LTEIAWKHDVQVMIEGPGHVPMHLIRENVEKQLKLCHEAPFYTLGPLVTDISPGYDHISSAIGAAMIGWFGTAMLCYVTP
KEHLGLPDRDDVKAGVISYKIAAHAADLAKGHPAARIRDDALSRARFSFRWRDQFALSLDPETAEAYHDETLPAEGAKVA
HFCSMCGPKFCSMKISQELKIEAGDIAQSGMAEMAEKFRASGGEIYQPAPDVAAPPSA

Sequences:

>Translated_618_residues
MKEIRMLDEASTSALKVTTGPLPHSRKVFIPAENPAVKVAMREIGLSDPSERPVRVYDTSGPYTDPNAIIDLTKGLPELR
RQWVLDRGDVEEVEGRAQKPEDDGLKPGQVSHLPAFDRTGRKPLRAKAGKRPTQMAYAKAGIVTPEMEYVAARENLARAN
GGAHPKHQAMGLGAAIPADITGEFVRQEVARGRAIIPSNINHLETEPMAIGRNFLVKINANIGNSAVTSGVAEEVDKLVW
AIRWGGDTVMDLSTGKNIHTIREWIIRNAPVPIGTVPIYQALEKVNGVVEDLNWEIFRDTVIEQAEQGVDYITVHAGVRL
PYIPLTANRVTGIVSRGGSIMAKWCLAHHKESFLYEHFEELCEILAQYDVAFSLGDGLRPGSIADANDAAQFAELETLGE
LTEIAWKHDVQVMIEGPGHVPMHLIRENVEKQLKLCHEAPFYTLGPLVTDISPGYDHISSAIGAAMIGWFGTAMLCYVTP
KEHLGLPDRDDVKAGVISYKIAAHAADLAKGHPAARIRDDALSRARFSFRWRDQFALSLDPETAEAYHDETLPAEGAKVA
HFCSMCGPKFCSMKISQELKIEAGDIAQSGMAEMAEKFRASGGEIYQPAPDVAAPPSA
>Mature_618_residues
MKEIRMLDEASTSALKVTTGPLPHSRKVFIPAENPAVKVAMREIGLSDPSERPVRVYDTSGPYTDPNAIIDLTKGLPELR
RQWVLDRGDVEEVEGRAQKPEDDGLKPGQVSHLPAFDRTGRKPLRAKAGKRPTQMAYAKAGIVTPEMEYVAARENLARAN
GGAHPKHQAMGLGAAIPADITGEFVRQEVARGRAIIPSNINHLETEPMAIGRNFLVKINANIGNSAVTSGVAEEVDKLVW
AIRWGGDTVMDLSTGKNIHTIREWIIRNAPVPIGTVPIYQALEKVNGVVEDLNWEIFRDTVIEQAEQGVDYITVHAGVRL
PYIPLTANRVTGIVSRGGSIMAKWCLAHHKESFLYEHFEELCEILAQYDVAFSLGDGLRPGSIADANDAAQFAELETLGE
LTEIAWKHDVQVMIEGPGHVPMHLIRENVEKQLKLCHEAPFYTLGPLVTDISPGYDHISSAIGAAMIGWFGTAMLCYVTP
KEHLGLPDRDDVKAGVISYKIAAHAADLAKGHPAARIRDDALSRARFSFRWRDQFALSLDPETAEAYHDETLPAEGAKVA
HFCSMCGPKFCSMKISQELKIEAGDIAQSGMAEMAEKFRASGGEIYQPAPDVAAPPSA

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=606, Percent_Identity=65.3465346534654, Blast_Score=826, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 67513; Mature: 67513

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEIRMLDEASTSALKVTTGPLPHSRKVFIPAENPAVKVAMREIGLSDPSERPVRVYDTS
CCCHHHHHCCCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCEEEEECC
GPYTDPNAIIDLTKGLPELRRQWVLDRGDVEEVEGRAQKPEDDGLKPGQVSHLPAFDRTG
CCCCCCHHHHHHHCCCHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
RKPLRAKAGKRPTQMAYAKAGIVTPEMEYVAARENLARANGGAHPKHQAMGLGAAIPADI
CCCHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCC
TGEFVRQEVARGRAIIPSNINHLETEPMAIGRNFLVKINANIGNSAVTSGVAEEVDKLVW
HHHHHHHHHHCCCEECCCCCCCCCCCCCEECCEEEEEEECCCCCHHHHHHHHHHHHHHEE
AIRWGGDTVMDLSTGKNIHTIREWIIRNAPVPIGTVPIYQALEKVNGVVEDLNWEIFRDT
EEEECCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
VIEQAEQGVDYITVHAGVRLPYIPLTANRVTGIVSRGGSIMAKWCLAHHKESFLYEHFEE
HHHHHHCCCCEEEEECCCCCCEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LCEILAQYDVAFSLGDGLRPGSIADANDAAQFAELETLGELTEIAWKHDVQVMIEGPGHV
HHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
PMHLIRENVEKQLKLCHEAPFYTLGPLVTDISPGYDHISSAIGAAMIGWFGTAMLCYVTP
HHHHHHHHHHHHHHHHHCCCCHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEECC
KEHLGLPDRDDVKAGVISYKIAAHAADLAKGHPAARIRDDALSRARFSFRWRDQFALSLD
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEECCCEEEEEC
PETAEAYHDETLPAEGAKVAHFCSMCGPKFCSMKISQELKIEAGDIAQSGMAEMAEKFRA
CCHHHHHCCCCCCCCCCHHHHHHHHCCCHHHCEECCCCEEEECCHHHHHHHHHHHHHHHC
SGGEIYQPAPDVAAPPSA
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKEIRMLDEASTSALKVTTGPLPHSRKVFIPAENPAVKVAMREIGLSDPSERPVRVYDTS
CCCHHHHHCCCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCEEEEECC
GPYTDPNAIIDLTKGLPELRRQWVLDRGDVEEVEGRAQKPEDDGLKPGQVSHLPAFDRTG
CCCCCCHHHHHHHCCCHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
RKPLRAKAGKRPTQMAYAKAGIVTPEMEYVAARENLARANGGAHPKHQAMGLGAAIPADI
CCCHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCC
TGEFVRQEVARGRAIIPSNINHLETEPMAIGRNFLVKINANIGNSAVTSGVAEEVDKLVW
HHHHHHHHHHCCCEECCCCCCCCCCCCCEECCEEEEEEECCCCCHHHHHHHHHHHHHHEE
AIRWGGDTVMDLSTGKNIHTIREWIIRNAPVPIGTVPIYQALEKVNGVVEDLNWEIFRDT
EEEECCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
VIEQAEQGVDYITVHAGVRLPYIPLTANRVTGIVSRGGSIMAKWCLAHHKESFLYEHFEE
HHHHHHCCCCEEEEECCCCCCEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LCEILAQYDVAFSLGDGLRPGSIADANDAAQFAELETLGELTEIAWKHDVQVMIEGPGHV
HHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
PMHLIRENVEKQLKLCHEAPFYTLGPLVTDISPGYDHISSAIGAAMIGWFGTAMLCYVTP
HHHHHHHHHHHHHHHHHCCCCHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEECC
KEHLGLPDRDDVKAGVISYKIAAHAADLAKGHPAARIRDDALSRARFSFRWRDQFALSLD
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEECCCEEEEEC
PETAEAYHDETLPAEGAKVAHFCSMCGPKFCSMKISQELKIEAGDIAQSGMAEMAEKFRA
CCHHHHHCCCCCCCCCCHHHHHHHHCCCHHHCEECCCCEEEECCHHHHHHHHHHHHHHHC
SGGEIYQPAPDVAAPPSA
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA