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The map label for this gene is tuf1

Identifier: 158424180

GI number: 158424180

Start: 2881682

End: 2882875

Strand: Reverse

Name: tuf1

Synonym: AZC_2556

Alternate gene names: 158424180

Gene position: 2882875-2881682 (Counterclockwise)

Preceding gene: 158424181

Following gene: 158424179

Centisome position: 53.69

GC content: 65.33

Gene sequence:

>1194_bases
ATGGCCAAAGCTAAGTTTGAACGTAACAAGCCGCACTGCAACATTGGCACCATCGGCCACGTTGACCACGGCAAGACCTC
TCTGACGGCCGCGATCACGAAGGTGCTCGCCGAGACGTCGGGTGGCGCGACCTTCACCGCCTACGACCAGATCGACAAGG
CGCCGGAAGAGAAGGCGCGCGGCATCACGATCTCGACGGCGCACGTCGAGTACGAGACGCAGAACCGCCACTATGCGCAC
GTGGACTGCCCCGGCCACGCCGACTATGTGAAGAACATGATCACCGGCGCGGCCCAGATGGACGGCGCGATCCTGGTGGT
GTCCGCCGCCGACGGCCCGATGCCGCAGACCCGCGAGCACATCCTGCTCGCCCGTCAGGTCGGCGTGCCGGCTCTGGTGG
TGTTCCTGAACAAGTGCGACATGGTCGACGATCCGGAGCTTCTTGAGCTGGTCGAGCTCGAGGTCCGCGAGCTTCTGTCC
AAGTACGACTTCCCCGGCGACGACATCCCGATCGTGCGCGGCTCTGCCCTGTGCGCGCTCGAGAACAAGTCTCCCGAGCT
TGGCGCCGAAGCCATCCTGAAGCTGATGGCCGAGGTGGACAAGTACATCCCGCAGCCGGAGCGTCCGGTTGACCAGCCCT
TCCTGATGCCGATCGAAGACGTGTTCTCGATCTCCGGCCGCGGCACCGTGGTGACCGGCCGTGTCGAGCGCGGCATCGTG
AAGGTGGGCGACGAAGTCGAGATCGTCGGCATCCGCCCGACGGTGAAGACCACCGTCACCGGCATCGAGATGTTCCGCAA
GCTGCTCGACCAGGGCCAGGCCGGCGACAACGTCGGCGTGCTGCTGCGCGGCACGAAGCGCGAGGACGTGGAGCGTGGCC
AGGTGGTGTGCAAGCCGGGTTCGGTGAAGCCGCACACCAAGTTCAAGGCCGAAGCCTACATCCTGACGAAGGAAGAGGGC
GGCCGTCATACGCCGTTCTTCACCAACTACCGCCCGCAGTTCTACTTCCGCACGACGGACGTGACCGGCGTGGTGACGCT
GCCCGAAGGCACCGAGATGGTGATGCCGGGCGACAACATCTCGGTGGACGTGCAGCTGATCGTCCCGATCGCGATGGAAG
AGAAGCTCCGCTTCGCCATCCGTGAGGGCGGCCGCACCGTCGGCGCCGGCGTCGTCGCCTCCATCATCGAGTGA

Upstream 100 bases:

>100_bases
GAACGTCGCTCAGGAGGTTCAGGCGAAATACGCCTGAACTTCTTAAGTCCTTATCGTTTTCGCAAGAAATCACCGCCAGA
GAGACCCAACGGAGAGTCCC

Downstream 100 bases:

>100_bases
AACAATAAGAAACGGGGCCGCGCCGCCAGCGCGGCGCGTCCCTCTTTTTCTTGGAGCCCTTGAGCCATGAACGGCCAGAA
CATTCGGATCCGCCTTAAGG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MAKAKFERNKPHCNIGTIGHVDHGKTSLTAAITKVLAETSGGATFTAYDQIDKAPEEKARGITISTAHVEYETQNRHYAH
VDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFLNKCDMVDDPELLELVELEVRELLS
KYDFPGDDIPIVRGSALCALENKSPELGAEAILKLMAEVDKYIPQPERPVDQPFLMPIEDVFSISGRGTVVTGRVERGIV
KVGDEVEIVGIRPTVKTTVTGIEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVVCKPGSVKPHTKFKAEAYILTKEEG
GRHTPFFTNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNISVDVQLIVPIAMEEKLRFAIREGGRTVGAGVVASIIE

Sequences:

>Translated_397_residues
MAKAKFERNKPHCNIGTIGHVDHGKTSLTAAITKVLAETSGGATFTAYDQIDKAPEEKARGITISTAHVEYETQNRHYAH
VDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFLNKCDMVDDPELLELVELEVRELLS
KYDFPGDDIPIVRGSALCALENKSPELGAEAILKLMAEVDKYIPQPERPVDQPFLMPIEDVFSISGRGTVVTGRVERGIV
KVGDEVEIVGIRPTVKTTVTGIEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVVCKPGSVKPHTKFKAEAYILTKEEG
GRHTPFFTNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNISVDVQLIVPIAMEEKLRFAIREGGRTVGAGVVASIIE
>Mature_396_residues
AKAKFERNKPHCNIGTIGHVDHGKTSLTAAITKVLAETSGGATFTAYDQIDKAPEEKARGITISTAHVEYETQNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFLNKCDMVDDPELLELVELEVRELLSK
YDFPGDDIPIVRGSALCALENKSPELGAEAILKLMAEVDKYIPQPERPVDQPFLMPIEDVFSISGRGTVVTGRVERGIVK
VGDEVEIVGIRPTVKTTVTGIEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVVCKPGSVKPHTKFKAEAYILTKEEGG
RHTPFFTNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNISVDVQLIVPIAMEEKLRFAIREGGRTVGAGVVASIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=57.9746835443038, Blast_Score=495, Evalue=1e-140,
Organism=Homo sapiens, GI223555963, Length=441, Percent_Identity=31.9727891156463, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI5729864, Length=441, Percent_Identity=31.9727891156463, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI4503471, Length=442, Percent_Identity=29.6380090497738, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI4503475, Length=443, Percent_Identity=29.1196388261851, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI194018520, Length=323, Percent_Identity=30.3405572755418, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI194097354, Length=323, Percent_Identity=30.3405572755418, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI194018522, Length=323, Percent_Identity=30.3405572755418, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI46094014, Length=323, Percent_Identity=30.6501547987616, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI54607086, Length=318, Percent_Identity=29.559748427673, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI4503507, Length=273, Percent_Identity=28.2051282051282, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI157426893, Length=289, Percent_Identity=25.9515570934256, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI53729339, Length=233, Percent_Identity=31.7596566523605, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI53729337, Length=233, Percent_Identity=31.7596566523605, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=77.5252525252525, Blast_Score=603, Evalue=1e-174,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=77.5252525252525, Blast_Score=603, Evalue=1e-174,
Organism=Escherichia coli, GI2367247, Length=381, Percent_Identity=30.4461942257218, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI48994988, Length=278, Percent_Identity=33.4532374100719, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789108, Length=330, Percent_Identity=28.4848484848485, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17556456, Length=401, Percent_Identity=54.3640897755611, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI25141371, Length=388, Percent_Identity=44.0721649484536, Blast_Score=331, Evalue=4e-91,
Organism=Caenorhabditis elegans, GI17552884, Length=444, Percent_Identity=30.1801801801802, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17569207, Length=444, Percent_Identity=30.1801801801802, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI32566303, Length=427, Percent_Identity=29.2740046838407, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI115532067, Length=439, Percent_Identity=28.4738041002278, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=434, Percent_Identity=28.5714285714286, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=439, Percent_Identity=27.1070615034169, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17506081, Length=299, Percent_Identity=29.0969899665552, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=38.0952380952381, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=310, Percent_Identity=26.4516129032258, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=241, Percent_Identity=26.5560165975104, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=399, Percent_Identity=67.4185463659148, Blast_Score=551, Evalue=1e-158,
Organism=Saccharomyces cerevisiae, GI6325337, Length=442, Percent_Identity=30.9954751131222, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=442, Percent_Identity=30.9954751131222, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=351, Percent_Identity=25.9259259259259, Blast_Score=99, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6320377, Length=342, Percent_Identity=27.7777777777778, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=282, Percent_Identity=28.0141843971631, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323320, Length=239, Percent_Identity=27.1966527196653, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=173, Percent_Identity=32.9479768786127, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322359, Length=134, Percent_Identity=30.5970149253731, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI281363316, Length=394, Percent_Identity=59.8984771573604, Blast_Score=502, Evalue=1e-142,
Organism=Drosophila melanogaster, GI17864358, Length=394, Percent_Identity=59.8984771573604, Blast_Score=502, Evalue=1e-142,
Organism=Drosophila melanogaster, GI19921738, Length=385, Percent_Identity=50.1298701298701, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI45553807, Length=443, Percent_Identity=28.8939051918736, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI45553816, Length=443, Percent_Identity=28.8939051918736, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24651721, Length=443, Percent_Identity=28.8939051918736, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI17864154, Length=443, Percent_Identity=28.8939051918736, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24652838, Length=448, Percent_Identity=28.5714285714286, Blast_Score=154, Evalue=9e-38,
Organism=Drosophila melanogaster, GI17137572, Length=448, Percent_Identity=28.5714285714286, Blast_Score=154, Evalue=9e-38,
Organism=Drosophila melanogaster, GI17137380, Length=345, Percent_Identity=30.4347826086957, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19922690, Length=304, Percent_Identity=31.25, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI45550900, Length=337, Percent_Identity=29.3768545994065, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24647048, Length=290, Percent_Identity=26.2068965517241, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24647046, Length=290, Percent_Identity=26.2068965517241, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28572034, Length=181, Percent_Identity=34.2541436464088, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_AZOC5 (A8HTW6)

Other databases:

- EMBL:   AP009384
- EMBL:   AP009384
- RefSeq:   YP_001523796.1
- RefSeq:   YP_001525472.1
- ProteinModelPortal:   A8HTW6
- SMR:   A8HTW6
- GeneID:   5690343
- GeneID:   5692332
- GenomeReviews:   AP009384_GR
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_0880
- KEGG:   azc:AZC_2556
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- BioCyc:   ACAU438753:AZC_0880-MONOMER
- BioCyc:   ACAU438753:AZC_2556-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43315; Mature: 43184

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERNKPHCNIGTIGHVDHGKTSLTAAITKVLAETSGGATFTAYDQIDKAPEEKAR
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHCCHHHHHC
GITISTAHVEYETQNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREH
CEEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHH
ILLARQVGVPALVVFLNKCDMVDDPELLELVELEVRELLSKYDFPGDDIPIVRGSALCAL
EEEHHHCCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEE
ENKSPELGAEAILKLMAEVDKYIPQPERPVDQPFLMPIEDVFSISGRGTVVTGRVERGIV
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECHHCCCEE
KVGDEVEIVGIRPTVKTTVTGIEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVVCKPG
EECCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCC
SVKPHTKFKAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNI
CCCCCCCEEEEEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCC
SVDVQLIVPIAMEEKLRFAIREGGRTVGAGVVASIIE
EEEEEEEEEEHHHHHHHHHHHHCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKAKFERNKPHCNIGTIGHVDHGKTSLTAAITKVLAETSGGATFTAYDQIDKAPEEKAR
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHCCHHHHHC
GITISTAHVEYETQNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREH
CEEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHH
ILLARQVGVPALVVFLNKCDMVDDPELLELVELEVRELLSKYDFPGDDIPIVRGSALCAL
EEEHHHCCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEE
ENKSPELGAEAILKLMAEVDKYIPQPERPVDQPFLMPIEDVFSISGRGTVVTGRVERGIV
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECHHCCCEE
KVGDEVEIVGIRPTVKTTVTGIEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVVCKPG
EECCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCC
SVKPHTKFKAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNI
CCCCCCCEEEEEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCC
SVDVQLIVPIAMEEKLRFAIREGGRTVGAGVVASIIE
EEEEEEEEEEHHHHHHHHHHHHCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA