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The map label for this gene is rpoA

Identifier: 158424154

GI number: 158424154

Start: 2867269

End: 2868294

Strand: Reverse

Name: rpoA

Synonym: AZC_2530

Alternate gene names: 158424154

Gene position: 2868294-2867269 (Counterclockwise)

Preceding gene: 158424155

Following gene: 158424153

Centisome position: 53.42

GC content: 63.06

Gene sequence:

>1026_bases
GTGACGCAAGTGATTCAGAAGAACTGGCAAGAGCTGATCAAGCCCGAGAAGCTGCAGGTGACTGCCGGCGCCGATCCCAA
GCGCCTCGCCACCGTCGTGGCCGAGCCGCTGGAGCGCGGCTTCGGCATGACGCTCGGCAATTCGCTGCGCCGGATCCTGC
TGTCGTCCCTTCAGGGCGCGGCGGTGACGTCGGTGCACATCGATGGCGTGCTGCACGAGTTCTCGTCCATTCCGGGCGTG
CGTGAGGACGTCACCGACATCATCCTCAACATCAAGGACATCGCCATCAAGATGCAGGGCGATGGCCCCAAGCGTCTCGT
GGCGAAGAAGAACGGTCCCGGCGTCCTCGTCGCCGGCGACATCCAGACCGTGGGCGACATCCAGATCCTGAACCCCCACC
TCGTGCTCTGCACGCTGGACGAGGGCGCGGAACTGCGCATGGAATTCACCGTCGATACCGGCAAGGGCTATGTCCCCGCC
GAACGCAACCGTCCCGAGGATGCTCCGATCGGCCTCATCCCGATCGACAGCCTCTACTCGCCGGTCCGCAAGGTCTCCTA
CAAGGTCGAGAACACCCGTGAGGGTCAGATCCTCGACTATGACAAGCTGACCATGCAGATCGAGACCGACGGCTCGGTGT
CCGCCGAGGACGCGCTCGCCTATGCGGCGCGCATCCTGCAGGACCAGCTTGAGATCTTCGTGAACTTCGAGGAGCCCAAG
CGCGAGAGCGAGACCGCTGCCATCCAGGAACTGCCCTTCAACCCGGCGCTCCTGAAGAAGGTGGACGAGCTCGAACTGTC
GGTGCGTTCGGCGAACTGCCTGAAGAACGACAACATCGTCTACATCGGCGACCTGATCCAGAAGAGCGAGACGGAGATGC
TCCGTACGCCGAATTTCGGCCGCAAGTCGCTCAACGAGATCAAGGAAGTGCTGGCCTCCATGGGCCTGCACCTCGGCATG
GAAGTGCCGGGCTGGCCGCCGGAGAACATCGAAGAGCTCGCGAAGCGCTTCGAGGACCACTACTGA

Upstream 100 bases:

>100_bases
GCTCGGTTCATCCCCGGCGAGCGGGGCGGGCCGGCGCCGCACAGGACCAGCGGGGTTTCCGTCCGGTTGATCGAGTGCCG
CCGCCCGGCGAGAAGGCAAG

Downstream 100 bases:

>100_bases
CCATTCACCCCGCGGCTCGAGCCGCTTAACCAATCTCGGCTCGAAACGCCGGAGGCATTTAGGAGTTCGCCGTCATGCGT
CACGGCAAGGTTCACCGCAA

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MTQVIQKNWQELIKPEKLQVTAGADPKRLATVVAEPLERGFGMTLGNSLRRILLSSLQGAAVTSVHIDGVLHEFSSIPGV
REDVTDIILNIKDIAIKMQGDGPKRLVAKKNGPGVLVAGDIQTVGDIQILNPHLVLCTLDEGAELRMEFTVDTGKGYVPA
ERNRPEDAPIGLIPIDSLYSPVRKVSYKVENTREGQILDYDKLTMQIETDGSVSAEDALAYAARILQDQLEIFVNFEEPK
RESETAAIQELPFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKSETEMLRTPNFGRKSLNEIKEVLASMGLHLGM
EVPGWPPENIEELAKRFEDHY

Sequences:

>Translated_341_residues
MTQVIQKNWQELIKPEKLQVTAGADPKRLATVVAEPLERGFGMTLGNSLRRILLSSLQGAAVTSVHIDGVLHEFSSIPGV
REDVTDIILNIKDIAIKMQGDGPKRLVAKKNGPGVLVAGDIQTVGDIQILNPHLVLCTLDEGAELRMEFTVDTGKGYVPA
ERNRPEDAPIGLIPIDSLYSPVRKVSYKVENTREGQILDYDKLTMQIETDGSVSAEDALAYAARILQDQLEIFVNFEEPK
RESETAAIQELPFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKSETEMLRTPNFGRKSLNEIKEVLASMGLHLGM
EVPGWPPENIEELAKRFEDHY
>Mature_340_residues
TQVIQKNWQELIKPEKLQVTAGADPKRLATVVAEPLERGFGMTLGNSLRRILLSSLQGAAVTSVHIDGVLHEFSSIPGVR
EDVTDIILNIKDIAIKMQGDGPKRLVAKKNGPGVLVAGDIQTVGDIQILNPHLVLCTLDEGAELRMEFTVDTGKGYVPAE
RNRPEDAPIGLIPIDSLYSPVRKVSYKVENTREGQILDYDKLTMQIETDGSVSAEDALAYAARILQDQLEIFVNFEEPKR
ESETAAIQELPFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKSETEMLRTPNFGRKSLNEIKEVLASMGLHLGME
VPGWPPENIEELAKRFEDHY

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=331, Percent_Identity=48.3383685800604, Blast_Score=298, Evalue=3e-82,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_AZOC5 (A8IAN5)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001525446.1
- ProteinModelPortal:   A8IAN5
- GeneID:   5688955
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_2530
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   ACAU438753:AZC_2530-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 37820; Mature: 37689

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQVIQKNWQELIKPEKLQVTAGADPKRLATVVAEPLERGFGMTLGNSLRRILLSSLQGA
CCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
AVTSVHIDGVLHEFSSIPGVREDVTDIILNIKDIAIKMQGDGPKRLVAKKNGPGVLVAGD
EEEEEEHHHHHHHHHHCCCCHHHHHHHHHEEEEEEEEECCCCCCEEEEECCCCEEEEECC
IQTVGDIQILNPHLVLCTLDEGAELRMEFTVDTGKGYVPAERNRPEDAPIGLIPIDSLYS
CCCCCCEEEECCEEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHH
PVRKVSYKVENTREGQILDYDKLTMQIETDGSVSAEDALAYAARILQDQLEIFVNFEEPK
HHHHHHEEECCCCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEECCCCCC
RESETAAIQELPFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKSETEMLRTPNFG
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHCCCCHHHHCCCCCC
RKSLNEIKEVLASMGLHLGMEVPGWPPENIEELAKRFEDHY
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQVIQKNWQELIKPEKLQVTAGADPKRLATVVAEPLERGFGMTLGNSLRRILLSSLQGA
CHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
AVTSVHIDGVLHEFSSIPGVREDVTDIILNIKDIAIKMQGDGPKRLVAKKNGPGVLVAGD
EEEEEEHHHHHHHHHHCCCCHHHHHHHHHEEEEEEEEECCCCCCEEEEECCCCEEEEECC
IQTVGDIQILNPHLVLCTLDEGAELRMEFTVDTGKGYVPAERNRPEDAPIGLIPIDSLYS
CCCCCCEEEECCEEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHH
PVRKVSYKVENTREGQILDYDKLTMQIETDGSVSAEDALAYAARILQDQLEIFVNFEEPK
HHHHHHEEECCCCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEECCCCCC
RESETAAIQELPFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKSETEMLRTPNFG
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHCCCCHHHHCCCCCC
RKSLNEIKEVLASMGLHLGMEVPGWPPENIEELAKRFEDHY
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA