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The map label for this gene is rutB

Identifier: 158424117

GI number: 158424117

Start: 2828638

End: 2829321

Strand: Direct

Name: rutB

Synonym: AZC_2493

Alternate gene names: 158424117

Gene position: 2828638-2829321 (Clockwise)

Preceding gene: 158424116

Following gene: 158424118

Centisome position: 52.68

GC content: 64.77

Gene sequence:

>684_bases
GTGCTGCCGGCCCGGCCCGAACCCCTGTCCGTGGCGGTGGAGGAAACCGCCGTCGTGGTCGTGGACATGCAGAATGCCTA
CGCCTCGCCCGGCGGCTATCTCGATATCGCCGGCTTCGACATTTCCGGCGCCAAGGCCGCCATTTCGGCCATCGCCGAGA
CGGTGGAGGCGGCCCGCGCGGCCGGCGTGACCGTGATCTATTTCCAGAACGGTTGGGACAAGGATTATGTGGAGGCGGGC
GGCCCCGGCTCGCCCAACTGGCACAAGTCCAATGCGCTGAAGACCATGCGCAAGAAGCCCGAGCTTCAGGGCCAGCTTCT
GGCGAAGGGCGGCTGGGACTATGCGCTGGTGGACCAGTTGGCCCCGCAGCCCGGCGACATCGTGGTGCCCAAGCCGCGCT
ATTCCGGCTTCTTCAACTCACAGTTCGACAGCATCCTGCGGGCGCGCGGCATCCGCAATCTCGTCTTCTGCGGCATCGCC
ACCAATGTCTGCGTGGAATCCACCCTGCGCGACGGCTTCCACCTCGAATATTTCGGCATCCTGCTGGAGGACGCGACCCA
TCAGGCCGGTCCCGAATACGTGCAGAAGGCCGCCATCTACAATGTGGAGACATTCTTCGGCTGGGTTTCCACCGTCGCCG
ATTTCCGCCGCGCCTTCGCGCAGATCCCTCCCAAGGAAGACTGA

Upstream 100 bases:

>100_bases
CCCAGATGGTGGACAATTCCTTCGCCCTGAAGGCGGCGAAGGATTTCGGAGTGGCCGGATGACCTTTGCGACCAGCTATC
CCCCGGCCCGCCGCACCGTC

Downstream 100 bases:

>100_bases
CCGGCCATGACCTCCCGCGCCATCATTCCCCCCGGCTCCGGCGTGCCGCTGGCGCCCTATTCGCCCGGTATGCAGGCGGA
CAATGTGATCTATGTCTCGG

Product: isochorismatase family protein

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MLPARPEPLSVAVEETAVVVVDMQNAYASPGGYLDIAGFDISGAKAAISAIAETVEAARAAGVTVIYFQNGWDKDYVEAG
GPGSPNWHKSNALKTMRKKPELQGQLLAKGGWDYALVDQLAPQPGDIVVPKPRYSGFFNSQFDSILRARGIRNLVFCGIA
TNVCVESTLRDGFHLEYFGILLEDATHQAGPEYVQKAAIYNVETFFGWVSTVADFRRAFAQIPPKED

Sequences:

>Translated_227_residues
MLPARPEPLSVAVEETAVVVVDMQNAYASPGGYLDIAGFDISGAKAAISAIAETVEAARAAGVTVIYFQNGWDKDYVEAG
GPGSPNWHKSNALKTMRKKPELQGQLLAKGGWDYALVDQLAPQPGDIVVPKPRYSGFFNSQFDSILRARGIRNLVFCGIA
TNVCVESTLRDGFHLEYFGILLEDATHQAGPEYVQKAAIYNVETFFGWVSTVADFRRAFAQIPPKED
>Mature_227_residues
MLPARPEPLSVAVEETAVVVVDMQNAYASPGGYLDIAGFDISGAKAAISAIAETVEAARAAGVTVIYFQNGWDKDYVEAG
GPGSPNWHKSNALKTMRKKPELQGQLLAKGGWDYALVDQLAPQPGDIVVPKPRYSGFFNSQFDSILRARGIRNLVFCGIA
TNVCVESTLRDGFHLEYFGILLEDATHQAGPEYVQKAAIYNVETFFGWVSTVADFRRAFAQIPPKED

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily

Homologues:

Organism=Escherichia coli, GI87081820, Length=219, Percent_Identity=71.2328767123288, Blast_Score=342, Evalue=1e-95,
Organism=Escherichia coli, GI87081992, Length=184, Percent_Identity=29.3478260869565, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUTB_AZOC5 (A8IAC9)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001525409.1
- ProteinModelPortal:   A8IAC9
- SMR:   A8IAC9
- GeneID:   5689537
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_2493
- HOGENOM:   HBG752434
- OMA:   MQNAYAS
- ProtClustDB:   CLSK879924
- BioCyc:   ACAU438753:AZC_2493-MONOMER
- HAMAP:   MF_00830
- InterPro:   IPR019916
- InterPro:   IPR000868
- Gene3D:   G3DSA:3.40.50.850
- TIGRFAMs:   TIGR03614

Pfam domain/function: PF00857 Isochorismatase; SSF52499 Iscrsm_hydrolase

EC number: NA

Molecular weight: Translated: 24689; Mature: 24689

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: ACT_SITE 164-164

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPARPEPLSVAVEETAVVVVDMQNAYASPGGYLDIAGFDISGAKAAISAIAETVEAARA
CCCCCCCCCEEEEECEEEEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
AGVTVIYFQNGWDKDYVEAGGPGSPNWHKSNALKTMRKKPELQGQLLAKGGWDYALVDQL
CCEEEEEEECCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
APQPGDIVVPKPRYSGFFNSQFDSILRARGIRNLVFCGIATNVCVESTLRDGFHLEYFGI
CCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCCCHHHHH
LLEDATHQAGPEYVQKAAIYNVETFFGWVSTVADFRRAFAQIPPKED
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLPARPEPLSVAVEETAVVVVDMQNAYASPGGYLDIAGFDISGAKAAISAIAETVEAARA
CCCCCCCCCEEEEECEEEEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
AGVTVIYFQNGWDKDYVEAGGPGSPNWHKSNALKTMRKKPELQGQLLAKGGWDYALVDQL
CCEEEEEEECCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
APQPGDIVVPKPRYSGFFNSQFDSILRARGIRNLVFCGIATNVCVESTLRDGFHLEYFGI
CCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCCCHHHHH
LLEDATHQAGPEYVQKAAIYNVETFFGWVSTVADFRRAFAQIPPKED
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA