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The map label for this gene is 158424112

Identifier: 158424112

GI number: 158424112

Start: 2824161

End: 2825093

Strand: Direct

Name: 158424112

Synonym: AZC_2488

Alternate gene names: NA

Gene position: 2824161-2825093 (Clockwise)

Preceding gene: 158424111

Following gene: 158424113

Centisome position: 52.59

GC content: 65.06

Gene sequence:

>933_bases
ATGCGTGAAATCAAGGTCGCGGCGGCCCAGTTGGGCGCCATCCAGAAGGCGGAGAGCCGGGAGGCCGTGGTCGGCCGCAT
GCTCGCCCTGATGGCCCAGGCGAAAGCCGCCGGCTGCAACCTCATCGTCTATCCCGAGCTCGCGCTCACCACTTTCTTCC
CGCGCTGGTACCACGAGAACCGCGCCGAGGCGGACGTGTGGTTCGAGAGCGCGATGCCCAACGAGGCGACCCAGCCGCTG
TTCGACGCCGCGCGCGAATATGGCATGGCCATGAGCTTCGGCTATGCGGAGCTGACCCCGGACGGCCACCACTTCAACAC
GTCCATCCTCGTGGACGACAAGGGCAACATCGTCGGCAAGTATCGCAAGATCCACCTGCCGGGCCATGCGGAATACGACC
ACGACCGCAGCCACCAGCATCTGGAGAAGCGCTATTTCGAGCCGGGCGACCTCGGCTTCAACGTGTGGCGCACCATGGGC
GGCATCTTCGGCATGGCGATCTGCAACGATCGCCGCTGGCCGGAGACCTATCGCGAGATGGGCCTTCAGGGCGTGGAGAT
GATCCTGCTGGGCTTCAACACCCCGTCGGTGAACGGCCAGAAGGGCGCCGAGGCCCATGCCCAGCGCATGTTCCACCACA
AGCTCTCCGTGCAAGCCGGCGCCTACCAGAATTCCACCTGGGTCATCGCCGTCGCCAAGGCCGGTTCCGAGGACGGCCAT
CACCTGATGGGCGGCACCGTCATCGTGAATCCGGATGGCGAGATCGTCGCGGAATCGAGGACCGAGGACGATGAACTGGT
GATCGCCGCCTGCGATCTCGATGCCACGGTTTTCGGCAAGGAGACGGTGTTCGATTTCGCCCGCCACCGCCGCATCGAAC
ACTATGGCCGCATCAACACCCAGACGGGCGTGATCCTGCCGCCGGAGGCCTGA

Upstream 100 bases:

>100_bases
GCGCCTCATGAGCCGGCTGGAACACCGGCTCGGAACCTGATCCCCTCTCAAGCGAAACGGCCCGGCGCGGCGGAGCGCAG
GCCCCCAGGAGTCTAGGAAG

Downstream 100 bases:

>100_bases
TCCCATGAGCGATACGGACGCGGCCGAAGGGCTGAGCTTTGATTTCGCCGCCACGGCGCCGGACCTGCGCTACAAGCTGC
TGACCGGCCTCGTGGTGCCG

Product: N-carbamyl-D-amino acid amidohydrolase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MREIKVAAAQLGAIQKAESREAVVGRMLALMAQAKAAGCNLIVYPELALTTFFPRWYHENRAEADVWFESAMPNEATQPL
FDAAREYGMAMSFGYAELTPDGHHFNTSILVDDKGNIVGKYRKIHLPGHAEYDHDRSHQHLEKRYFEPGDLGFNVWRTMG
GIFGMAICNDRRWPETYREMGLQGVEMILLGFNTPSVNGQKGAEAHAQRMFHHKLSVQAGAYQNSTWVIAVAKAGSEDGH
HLMGGTVIVNPDGEIVAESRTEDDELVIAACDLDATVFGKETVFDFARHRRIEHYGRINTQTGVILPPEA

Sequences:

>Translated_310_residues
MREIKVAAAQLGAIQKAESREAVVGRMLALMAQAKAAGCNLIVYPELALTTFFPRWYHENRAEADVWFESAMPNEATQPL
FDAAREYGMAMSFGYAELTPDGHHFNTSILVDDKGNIVGKYRKIHLPGHAEYDHDRSHQHLEKRYFEPGDLGFNVWRTMG
GIFGMAICNDRRWPETYREMGLQGVEMILLGFNTPSVNGQKGAEAHAQRMFHHKLSVQAGAYQNSTWVIAVAKAGSEDGH
HLMGGTVIVNPDGEIVAESRTEDDELVIAACDLDATVFGKETVFDFARHRRIEHYGRINTQTGVILPPEA
>Mature_310_residues
MREIKVAAAQLGAIQKAESREAVVGRMLALMAQAKAAGCNLIVYPELALTTFFPRWYHENRAEADVWFESAMPNEATQPL
FDAAREYGMAMSFGYAELTPDGHHFNTSILVDDKGNIVGKYRKIHLPGHAEYDHDRSHQHLEKRYFEPGDLGFNVWRTMG
GIFGMAICNDRRWPETYREMGLQGVEMILLGFNTPSVNGQKGAEAHAQRMFHHKLSVQAGAYQNSTWVIAVAKAGSEDGH
HLMGGTVIVNPDGEIVAESRTEDDELVIAACDLDATVFGKETVFDFARHRRIEHYGRINTQTGVILPPEA

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=302, Percent_Identity=23.1788079470199, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323383, Length=305, Percent_Identity=25.5737704918033, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21358471, Length=297, Percent_Identity=24.2424242424242, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 34573; Mature: 34573

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS50263 CN_HYDROLASE ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREIKVAAAQLGAIQKAESREAVVGRMLALMAQAKAAGCNLIVYPELALTTFFPRWYHEN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCC
RAEADVWFESAMPNEATQPLFDAAREYGMAMSFGYAELTPDGHHFNTSILVDDKGNIVGK
CCCHHEEECCCCCCCCCHHHHHHHHHCCCEEECCCEEECCCCCEEEEEEEEECCCCEEEE
YRKIHLPGHAEYDHDRSHQHLEKRYFEPGDLGFNVWRTMGGIFGMAICNDRRWPETYREM
EEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEEEECCCCCHHHHHHC
GLQGVEMILLGFNTPSVNGQKGAEAHAQRMFHHKLSVQAGAYQNSTWVIAVAKAGSEDGH
CCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCEEEEEEEECCCCCCC
HLMGGTVIVNPDGEIVAESRTEDDELVIAACDLDATVFGKETVFDFARHRRIEHYGRINT
EEECCEEEECCCCCEEECCCCCCCCEEEEEECCCCEECCHHHHHHHHHHHHHHHHCCCCC
QTGVILPPEA
CCCEEECCCC
>Mature Secondary Structure
MREIKVAAAQLGAIQKAESREAVVGRMLALMAQAKAAGCNLIVYPELALTTFFPRWYHEN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCC
RAEADVWFESAMPNEATQPLFDAAREYGMAMSFGYAELTPDGHHFNTSILVDDKGNIVGK
CCCHHEEECCCCCCCCCHHHHHHHHHCCCEEECCCEEECCCCCEEEEEEEEECCCCEEEE
YRKIHLPGHAEYDHDRSHQHLEKRYFEPGDLGFNVWRTMGGIFGMAICNDRRWPETYREM
EEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEEEECCCCCHHHHHHC
GLQGVEMILLGFNTPSVNGQKGAEAHAQRMFHHKLSVQAGAYQNSTWVIAVAKAGSEDGH
CCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCEEEEEEEECCCCCCC
HLMGGTVIVNPDGEIVAESRTEDDELVIAACDLDATVFGKETVFDFARHRRIEHYGRINT
EEECCEEEECCCCCEEECCCCCCCCEEEEEECCCCEECCHHHHHHHHHHHHHHHHCCCCC
QTGVILPPEA
CCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]