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The map label for this gene is rnhB

Identifier: 158424022

GI number: 158424022

Start: 2725711

End: 2726490

Strand: Reverse

Name: rnhB

Synonym: AZC_2398

Alternate gene names: 158424022

Gene position: 2726490-2725711 (Counterclockwise)

Preceding gene: 158424025

Following gene: 158424019

Centisome position: 50.77

GC content: 68.85

Gene sequence:

>780_bases
GTGCTCAGCACTCCTCCGAAGTTGCCAAGTGCCCACGGCCCCGTGCATTTTCCCCGACGTTCTGGAACGGGGATGAATCG
CATGGCGCGCTCGGCCACGGCCGTGAAGACACTGGCCGGCCTCAGCTTCAAGCGGGAGCGGGCGGTCCTGAAGTCAGGGA
TGGCACCGGTCGCCGGCGCGGACGAGGCGGGACGGGGTCCACTCGCCGGTCCGGTGGTCGCGGCCGCCGTCATTCTCGAT
CCCAAGCGCATTCCGGCCGGCCTCGACGATTCGAAGAAGCTCACCGCCACCCGGCGCGAGGCGCTGTTCGACGAGATTTG
CGCCACTGCCGAGGTTTCGGTGGTGATGGCGCCGCCGAGCCGCATCGATCGCGACAACATCCGGCAGGCGACGCTCTGGG
CGCTGGCCAATGCGGTGAAGGGCCTTCCTGAAGACCCGCGCCTCGTCTTCGTGGACGGCAACGACCGGCCTCCGCTCGAA
TGCGAGGTGGAGATGGTCATCGGCGGCGACGGGCTGATCGCCTCCATCGCAGCCGCCTCGATCGTCGCCAAGGTGACGCG
CGACCGCCTCATGGTGGGGCTTGGTGCGGCCTTTCCGGGCTATGGATTCGAGCGTCACATGGGCTACGGCACCGCGGAGC
ACGGGGCGGCGCTGCGCCGGCTCGGCCCCTGCCGGCACCACCGCACGTCTTTCGCACCGGTGCGGGCGCAGCAGCTTGTG
CTCTTCGAGACGGAAATGCTGGAGATCGAAGCCGAGCCGCTGGTCGACGCGCTCGTGTGA

Upstream 100 bases:

>100_bases
CCGAGGTCAACCTCCGGCCAGCCCCACGGGTTCATCAGGCAAACGCACGGATGAGTGGCCCCCGCGCCGGTGGCCGTCAA
GCACCGGCCTGCGACCGTCT

Downstream 100 bases:

>100_bases
CACGCAACATCGCGGCCTTCGGCGCCAGCGCGCGAACAGCAAAAAGCCCCTCCGACCCATGGCCGGAGGGGCTCTGCGTT
TCGCGACAAGCTCAGGCCTT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MLSTPPKLPSAHGPVHFPRRSGTGMNRMARSATAVKTLAGLSFKRERAVLKSGMAPVAGADEAGRGPLAGPVVAAAVILD
PKRIPAGLDDSKKLTATRREALFDEICATAEVSVVMAPPSRIDRDNIRQATLWALANAVKGLPEDPRLVFVDGNDRPPLE
CEVEMVIGGDGLIASIAAASIVAKVTRDRLMVGLGAAFPGYGFERHMGYGTAEHGAALRRLGPCRHHRTSFAPVRAQQLV
LFETEMLEIEAEPLVDALV

Sequences:

>Translated_259_residues
MLSTPPKLPSAHGPVHFPRRSGTGMNRMARSATAVKTLAGLSFKRERAVLKSGMAPVAGADEAGRGPLAGPVVAAAVILD
PKRIPAGLDDSKKLTATRREALFDEICATAEVSVVMAPPSRIDRDNIRQATLWALANAVKGLPEDPRLVFVDGNDRPPLE
CEVEMVIGGDGLIASIAAASIVAKVTRDRLMVGLGAAFPGYGFERHMGYGTAEHGAALRRLGPCRHHRTSFAPVRAQQLV
LFETEMLEIEAEPLVDALV
>Mature_259_residues
MLSTPPKLPSAHGPVHFPRRSGTGMNRMARSATAVKTLAGLSFKRERAVLKSGMAPVAGADEAGRGPLAGPVVAAAVILD
PKRIPAGLDDSKKLTATRREALFDEICATAEVSVVMAPPSRIDRDNIRQATLWALANAVKGLPEDPRLVFVDGNDRPPLE
CEVEMVIGGDGLIASIAAASIVAKVTRDRLMVGLGAAFPGYGFERHMGYGTAEHGAALRRLGPCRHHRTSFAPVRAQQLV
LFETEMLEIEAEPLVDALV

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=180, Percent_Identity=51.6666666666667, Blast_Score=159, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_AZOC5 (A8I6E6)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001525314.1
- ProteinModelPortal:   A8I6E6
- SMR:   A8I6E6
- GeneID:   5689063
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_2398
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- BioCyc:   ACAU438753:AZC_2398-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 27525; Mature: 27525

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSTPPKLPSAHGPVHFPRRSGTGMNRMARSATAVKTLAGLSFKRERAVLKSGMAPVAGA
CCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCC
DEAGRGPLAGPVVAAAVILDPKRIPAGLDDSKKLTATRREALFDEICATAEVSVVMAPPS
CCCCCCCCCHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCC
RIDRDNIRQATLWALANAVKGLPEDPRLVFVDGNDRPPLECEVEMVIGGDGLIASIAAAS
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHH
IVAKVTRDRLMVGLGAAFPGYGFERHMGYGTAEHGAALRRLGPCRHHRTSFAPVRAQQLV
HHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHCCHHHCCCCCCCCCHHEEE
LFETEMLEIEAEPLVDALV
EEEHHHHEECHHHHHHHCC
>Mature Secondary Structure
MLSTPPKLPSAHGPVHFPRRSGTGMNRMARSATAVKTLAGLSFKRERAVLKSGMAPVAGA
CCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCC
DEAGRGPLAGPVVAAAVILDPKRIPAGLDDSKKLTATRREALFDEICATAEVSVVMAPPS
CCCCCCCCCHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCC
RIDRDNIRQATLWALANAVKGLPEDPRLVFVDGNDRPPLECEVEMVIGGDGLIASIAAAS
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHH
IVAKVTRDRLMVGLGAAFPGYGFERHMGYGTAEHGAALRRLGPCRHHRTSFAPVRAQQLV
HHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHCCHHHCCCCCCCCCHHEEE
LFETEMLEIEAEPLVDALV
EEEHHHHEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA