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The map label for this gene is yjeA [H]

Identifier: 158424020

GI number: 158424020

Start: 2723904

End: 2725052

Strand: Direct

Name: yjeA [H]

Synonym: AZC_2396

Alternate gene names: 158424020

Gene position: 2723904-2725052 (Clockwise)

Preceding gene: 158424015

Following gene: 158424021

Centisome position: 50.73

GC content: 69.19

Gene sequence:

>1149_bases
ATGTCGGCGACATGCCGCAGTGTTTCAGCCGGGCGCACCATAACGATCTTCGCGATTTGCGCCAGTCCTGCCCGGCAACC
CTGCGCCCGGGCCGCTGACATGACCCGCCCCAGCCCCTGGTGGCACCCGAGCGTCCATGAGGACCGCCGCCCCTTTCTCC
TCGCCCGCGCCCGCATCGCCGCAGCCATCCGCGCCGATTTCGCCGCCCGCGACTTTCTGGAAGTGGAAACGCCTGTGCTT
CAGGTGTCGCCGGGGAACGAGGCGCACCTGCACGCCTTCGCCACGCTCTGGCGGCGACCGGAGGGCGGCACCCTGCCCTA
TTATCTGCGCACCTCGCCGGAGTTTGCCGCCAAGAAGCTGCTGGCGGCCGGGGAGCCCCGCATCTTCGAATTCGCCCGTG
TCTTCCGCGACCGCGAGCGCGGCACGACGCACCATCCCGAGTTCACCATGCTGGAATGGTACCGGGCCCGCGAACCCTAC
GAGACCCTGATGGAGGACTGTGTCCGCCTGCTCCGCCTTGCTTCCAACGCGGGCGGCATCTCCGGTTTTTCGTTCCGGGG
GCGGAGCTGCGATCCCTTTGCCGAGGCCGAGCGGCTGACGCTCGTCGCGGCATTCGCGCGGCATGCGGGCATCGATCTGT
CCCGCTTTCTCGCGCCGCCGGGGCACGAGCCGGAAGCCGCTCCCTTTGCCGAGGCGGTGGCGGCGGCGGGCATCCGCACC
GCGCCGGACGACACTTGGGCGGACCTCTTCTCCCGTGTTCTCGTGGAGAAGGTGGAGCCGCATCTGGGCGACGGACGCCC
GACCCTGCTCTGCGAATATCCCACCAGCGAGGCGGCCCTCGCCCGTCCCACCGTGGCCGACCCGCGCGTCGCGGAACGTT
TCGAGCTCTATGCCTGCGGAGTCGAACTCGCCAATGCCTTCGGAGAACTGACGGACCCGGTGGAGCAGCGGCGGCGCTTC
GAGATCGAGATGGCGGAGAAGGCCCGCCTCTATGGCGAGCGCTATCCCATCGACGAGGGGCTTCTGGAGGCGCTGGAGCA
TATGCCCGAGGCGAGCGGCTGCGCGCTCGGCTTCGACCGCCTTGCTATGCTGGCGAGCGGTGCCCGGCGCCTCGACGACA
TGCTGTGGGCGCCAGTCGAGATTTCGTGA

Upstream 100 bases:

>100_bases
GCACTGAGAACCACGTAGAGCTTGTCATCGATCTCGACGACATTGCCCTTGCGCAGAGAGCTGGCGATGACCTTGACCAC
GTTTTTTCTTCCTCAGACAG

Downstream 100 bases:

>100_bases
TCCACGTTTTCGCCACATTTTGCGTTGCGTCAATCACAGACTGTTCTGCCCCGCTCAAGATCAGCCTCACTGATTAAGTG
GAGAACAGTCATGTCGATCG

Product: lysyl-tRNA synthetase

Products: AMP; diphosphate; L-lysyl-tRNALys [C]

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MSATCRSVSAGRTITIFAICASPARQPCARAADMTRPSPWWHPSVHEDRRPFLLARARIAAAIRADFAARDFLEVETPVL
QVSPGNEAHLHAFATLWRRPEGGTLPYYLRTSPEFAAKKLLAAGEPRIFEFARVFRDRERGTTHHPEFTMLEWYRAREPY
ETLMEDCVRLLRLASNAGGISGFSFRGRSCDPFAEAERLTLVAAFARHAGIDLSRFLAPPGHEPEAAPFAEAVAAAGIRT
APDDTWADLFSRVLVEKVEPHLGDGRPTLLCEYPTSEAALARPTVADPRVAERFELYACGVELANAFGELTDPVEQRRRF
EIEMAEKARLYGERYPIDEGLLEALEHMPEASGCALGFDRLAMLASGARRLDDMLWAPVEIS

Sequences:

>Translated_382_residues
MSATCRSVSAGRTITIFAICASPARQPCARAADMTRPSPWWHPSVHEDRRPFLLARARIAAAIRADFAARDFLEVETPVL
QVSPGNEAHLHAFATLWRRPEGGTLPYYLRTSPEFAAKKLLAAGEPRIFEFARVFRDRERGTTHHPEFTMLEWYRAREPY
ETLMEDCVRLLRLASNAGGISGFSFRGRSCDPFAEAERLTLVAAFARHAGIDLSRFLAPPGHEPEAAPFAEAVAAAGIRT
APDDTWADLFSRVLVEKVEPHLGDGRPTLLCEYPTSEAALARPTVADPRVAERFELYACGVELANAFGELTDPVEQRRRF
EIEMAEKARLYGERYPIDEGLLEALEHMPEASGCALGFDRLAMLASGARRLDDMLWAPVEIS
>Mature_381_residues
SATCRSVSAGRTITIFAICASPARQPCARAADMTRPSPWWHPSVHEDRRPFLLARARIAAAIRADFAARDFLEVETPVLQ
VSPGNEAHLHAFATLWRRPEGGTLPYYLRTSPEFAAKKLLAAGEPRIFEFARVFRDRERGTTHHPEFTMLEWYRAREPYE
TLMEDCVRLLRLASNAGGISGFSFRGRSCDPFAEAERLTLVAAFARHAGIDLSRFLAPPGHEPEAAPFAEAVAAAGIRTA
PDDTWADLFSRVLVEKVEPHLGDGRPTLLCEYPTSEAALARPTVADPRVAERFELYACGVELANAFGELTDPVEQRRRFE
IEMAEKARLYGERYPIDEGLLEALEHMPEASGCALGFDRLAMLASGARRLDDMLWAPVEIS

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=342, Percent_Identity=28.6549707602339, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI5031815, Length=342, Percent_Identity=28.6549707602339, Blast_Score=131, Evalue=1e-30,
Organism=Escherichia coli, GI87082379, Length=347, Percent_Identity=38.6167146974063, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1790571, Length=339, Percent_Identity=32.7433628318584, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1789256, Length=339, Percent_Identity=31.858407079646, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71994340, Length=339, Percent_Identity=29.4985250737463, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17535925, Length=339, Percent_Identity=29.4985250737463, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17535927, Length=339, Percent_Identity=29.4985250737463, Blast_Score=123, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6320242, Length=350, Percent_Identity=27.7142857142857, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24640851, Length=344, Percent_Identity=29.3604651162791, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24640849, Length=344, Percent_Identity=29.3604651162791, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6 [C]

Molecular weight: Translated: 42481; Mature: 42349

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATCRSVSAGRTITIFAICASPARQPCARAADMTRPSPWWHPSVHEDRRPFLLARARIA
CCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
AAIRADFAARDFLEVETPVLQVSPGNEAHLHAFATLWRRPEGGTLPYYLRTSPEFAAKKL
HHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHH
LAAGEPRIFEFARVFRDRERGTTHHPEFTMLEWYRAREPYETLMEDCVRLLRLASNAGGI
HHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
SGFSFRGRSCDPFAEAERLTLVAAFARHAGIDLSRFLAPPGHEPEAAPFAEAVAAAGIRT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCC
APDDTWADLFSRVLVEKVEPHLGDGRPTLLCEYPTSEAALARPTVADPRVAERFELYACG
CCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHCCCCCCCCHHHHHHHHHHHH
VELANAFGELTDPVEQRRRFEIEMAEKARLYGERYPIDEGLLEALEHMPEASGCALGFDR
HHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHH
LAMLASGARRLDDMLWAPVEIS
HHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SATCRSVSAGRTITIFAICASPARQPCARAADMTRPSPWWHPSVHEDRRPFLLARARIA
CCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
AAIRADFAARDFLEVETPVLQVSPGNEAHLHAFATLWRRPEGGTLPYYLRTSPEFAAKKL
HHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHH
LAAGEPRIFEFARVFRDRERGTTHHPEFTMLEWYRAREPYETLMEDCVRLLRLASNAGGI
HHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
SGFSFRGRSCDPFAEAERLTLVAAFARHAGIDLSRFLAPPGHEPEAAPFAEAVAAAGIRT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCC
APDDTWADLFSRVLVEKVEPHLGDGRPTLLCEYPTSEAALARPTVADPRVAERFELYACG
CCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHCCCCCCCCHHHHHHHHHHHH
VELANAFGELTDPVEQRRRFEIEMAEKARLYGERYPIDEGLLEALEHMPEASGCALGFDR
HHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHH
LAMLASGARRLDDMLWAPVEIS
HHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNALys [C]

Specific reaction: ATP + L-lysine + tRNALys = AMP + diphosphate + L-lysyl-tRNALys [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA