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The map label for this gene is ilvI [H]

Identifier: 158424000

GI number: 158424000

Start: 2702056

End: 2703825

Strand: Reverse

Name: ilvI [H]

Synonym: AZC_2376

Alternate gene names: 158424000

Gene position: 2703825-2702056 (Counterclockwise)

Preceding gene: 158424008

Following gene: 158423999

Centisome position: 50.35

GC content: 65.42

Gene sequence:

>1770_bases
GTGGACGACATGACCAAGGAGATGACCGGCGCCGAAATGGTGATCCAGGCGCTCACCGATCAGGGTGTCGAACACCTGTT
CGGTTATCCGGGCGGCGCGGTGCTGCCCATCTATGACGCCCTGTTCCACCAGAACCGGATCGAGCACATCCTCGTGCGCC
ACGAGCAGGCGGCTGTGCATTCGGCGGAGGGCTATGCCCGCTCGTCCGGCAAGGTCGGCGTCGTCCTCGTCACGTCCGGC
CCCGGTGCCACCAACGCGGTCACCGGCCTGACGGATGCCCTGATGGATTCGATCCCGATCGTCTGCATCACCGGCCAGGT
CGCGACACACCTCATCGGCAACGATGCCTTCCAGGAGTGCGACACGGTCGGCATCACCCGGCCCTGCACCAAGCACAATT
ATCTGGTGCGCGACGTCAACGATCTCGCCCGCGTGCTGCACGAGGCCTTCTATGTCGCCCAGAACGGGCGTCCGGGTCCG
GTGGTCGTGGACATCCCCAAGGATGTCCAGTTCGCCAAGGGCACCTACATCGGCCGCGAGAATATCGAGCACAAGACCTA
CAAGCCGCGGCTCGTGGGCGATGACGGCGCGGTCCGCCAGGCCATCGAGATGATCGCCGGCGCCAAGCGTCCGATCTTCT
ACACCGGCGGCGGCGTCATCAATTCCGGCCCGGCGGCAAGCCGTGCGCTGCGCGAGCTGGCGCGCCTCTCGGGCGTTCCG
GTCACCTCGACCCTGATGGGCCTCGGCGCCTTCCCCGCCGCCAGCAGCCAGTGGCTCGGCATGCTCGGCATGCACGGTAC
CTACGAAGCCAACATGGCCATGCACGGCTGCGACGTGATGGTCTGCATCGGCGCGCGGTTCGACGACCGCATCACCGGCC
GCCTCGACGCCTTCGCGCCGGGTTCGAAGAAGATCCACATCGACATCGACCCCTCGTCCATCAACAAGAACGTCAAGGTC
GACCTGCCCATCATCGGCGACTGCACCAAGGTGCTGGAGGCGATGGTTGCCCTCTGGAAGTCGCAGGACCGCCAGACCGA
CAAGGCGGCGCTCGACGAATGGTGGAAGCAGATCGACCGCTGGCGTGCGCGCAACTCGCTCGCCTACCGTCTGCTGCCGG
ACGTCATCAAGCCGCAGCAGGCCATCGAGCGCCTCTATGCGGCGACGAAGGACAAGGACGTCTACATCACCACCGAGGTC
GGCCAGCACCAGATGTGGGCGGCGCAGTTCTTCCGGTTCGAGGAGCCGAACCGCTGGATGACCTCCGGCGGCCTCGGCAC
CATGGGCTACGGCCTGCCCGCCGCCATCGGCGCGCAGATCGCCCATCCGGACGGCCTGTGCATCGACATCGCCGGCGAGG
CCTCCATCCTGATGAACATGCAGGAGATGTCGACGGCCGTGCAGTTCAACCTGCCGGTGAAGATCTTCATCCTGAACAAC
CGCTACATGGGCATGGTGCGCCAGTGGCAGGAACTGCTGCACGGCGGGCGCTACTCGCACTCCTATTCGGAGGCGCTGCC
CGACTTCGTGAAGCTCGCGGACGCTTATGGCGGCGTCGGCATCCGCTGCGAGAAGCCCGGCGACCTCGACGGGGCCATCC
AGGAGATGATCAACATCAAGCGCCCGGTGATCTTCGACTGCATCGTGGACCAGCAGGAAAACTGCTTCCCCATGATCCCG
TCCGGCCGCGCGCACAATGAGATGATCCTCGGCGACATGGCGGTCGATCTCGACGAGGCCATCACCGACGAAGGCAAGAT
GCTGGTGTGA

Upstream 100 bases:

>100_bases
GGCCCCTTCGTTGTTTCTGGAGCTCGGCTCCGGAGCCCACATATGGTGATCAATCGGGATTGAGGGCGCGCGAGGCGCCG
GGCAGCTGGGGCTGCGAGGA

Downstream 100 bases:

>100_bases
GCCTCCTGCCGCCGGGCGCGCCTTTGCGCCCGGCCTCGCCGCGCCCCTCCATTCCCTGTTTTCGCAGAGTCCCCTCGATG
TCGCTCGCGCAAGAGAAGAC

Product: acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 589; Mature: 589

Protein sequence:

>589_residues
MDDMTKEMTGAEMVIQALTDQGVEHLFGYPGGAVLPIYDALFHQNRIEHILVRHEQAAVHSAEGYARSSGKVGVVLVTSG
PGATNAVTGLTDALMDSIPIVCITGQVATHLIGNDAFQECDTVGITRPCTKHNYLVRDVNDLARVLHEAFYVAQNGRPGP
VVVDIPKDVQFAKGTYIGRENIEHKTYKPRLVGDDGAVRQAIEMIAGAKRPIFYTGGGVINSGPAASRALRELARLSGVP
VTSTLMGLGAFPAASSQWLGMLGMHGTYEANMAMHGCDVMVCIGARFDDRITGRLDAFAPGSKKIHIDIDPSSINKNVKV
DLPIIGDCTKVLEAMVALWKSQDRQTDKAALDEWWKQIDRWRARNSLAYRLLPDVIKPQQAIERLYAATKDKDVYITTEV
GQHQMWAAQFFRFEEPNRWMTSGGLGTMGYGLPAAIGAQIAHPDGLCIDIAGEASILMNMQEMSTAVQFNLPVKIFILNN
RYMGMVRQWQELLHGGRYSHSYSEALPDFVKLADAYGGVGIRCEKPGDLDGAIQEMINIKRPVIFDCIVDQQENCFPMIP
SGRAHNEMILGDMAVDLDEAITDEGKMLV

Sequences:

>Translated_589_residues
MDDMTKEMTGAEMVIQALTDQGVEHLFGYPGGAVLPIYDALFHQNRIEHILVRHEQAAVHSAEGYARSSGKVGVVLVTSG
PGATNAVTGLTDALMDSIPIVCITGQVATHLIGNDAFQECDTVGITRPCTKHNYLVRDVNDLARVLHEAFYVAQNGRPGP
VVVDIPKDVQFAKGTYIGRENIEHKTYKPRLVGDDGAVRQAIEMIAGAKRPIFYTGGGVINSGPAASRALRELARLSGVP
VTSTLMGLGAFPAASSQWLGMLGMHGTYEANMAMHGCDVMVCIGARFDDRITGRLDAFAPGSKKIHIDIDPSSINKNVKV
DLPIIGDCTKVLEAMVALWKSQDRQTDKAALDEWWKQIDRWRARNSLAYRLLPDVIKPQQAIERLYAATKDKDVYITTEV
GQHQMWAAQFFRFEEPNRWMTSGGLGTMGYGLPAAIGAQIAHPDGLCIDIAGEASILMNMQEMSTAVQFNLPVKIFILNN
RYMGMVRQWQELLHGGRYSHSYSEALPDFVKLADAYGGVGIRCEKPGDLDGAIQEMINIKRPVIFDCIVDQQENCFPMIP
SGRAHNEMILGDMAVDLDEAITDEGKMLV
>Mature_589_residues
MDDMTKEMTGAEMVIQALTDQGVEHLFGYPGGAVLPIYDALFHQNRIEHILVRHEQAAVHSAEGYARSSGKVGVVLVTSG
PGATNAVTGLTDALMDSIPIVCITGQVATHLIGNDAFQECDTVGITRPCTKHNYLVRDVNDLARVLHEAFYVAQNGRPGP
VVVDIPKDVQFAKGTYIGRENIEHKTYKPRLVGDDGAVRQAIEMIAGAKRPIFYTGGGVINSGPAASRALRELARLSGVP
VTSTLMGLGAFPAASSQWLGMLGMHGTYEANMAMHGCDVMVCIGARFDDRITGRLDAFAPGSKKIHIDIDPSSINKNVKV
DLPIIGDCTKVLEAMVALWKSQDRQTDKAALDEWWKQIDRWRARNSLAYRLLPDVIKPQQAIERLYAATKDKDVYITTEV
GQHQMWAAQFFRFEEPNRWMTSGGLGTMGYGLPAAIGAQIAHPDGLCIDIAGEASILMNMQEMSTAVQFNLPVKIFILNN
RYMGMVRQWQELLHGGRYSHSYSEALPDFVKLADAYGGVGIRCEKPGDLDGAIQEMINIKRPVIFDCIVDQQENCFPMIP
SGRAHNEMILGDMAVDLDEAITDEGKMLV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=566, Percent_Identity=24.7349823321555, Blast_Score=159, Evalue=9e-39,
Organism=Homo sapiens, GI21361361, Length=513, Percent_Identity=26.5107212475634, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=51.5845070422535, Blast_Score=614, Evalue=1e-177,
Organism=Escherichia coli, GI1790104, Length=567, Percent_Identity=42.1516754850088, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI1786717, Length=564, Percent_Identity=33.3333333333333, Blast_Score=302, Evalue=4e-83,
Organism=Escherichia coli, GI1787096, Length=561, Percent_Identity=27.807486631016, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1788716, Length=578, Percent_Identity=25.0865051903114, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17531299, Length=554, Percent_Identity=26.173285198556, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17531301, Length=554, Percent_Identity=26.173285198556, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17542570, Length=514, Percent_Identity=25.0972762645914, Blast_Score=124, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=603, Percent_Identity=44.9419568822554, Blast_Score=494, Evalue=1e-140,
Organism=Saccharomyces cerevisiae, GI6320816, Length=484, Percent_Identity=24.1735537190083, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19922626, Length=486, Percent_Identity=25.5144032921811, Blast_Score=139, Evalue=7e-33,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64533; Mature: 64533

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDMTKEMTGAEMVIQALTDQGVEHLFGYPGGAVLPIYDALFHQNRIEHILVRHEQAAVH
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAEGYARSSGKVGVVLVTSGPGATNAVTGLTDALMDSIPIVCITGQVATHLIGNDAFQEC
CCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECHHHHHHHCCHHHHHH
DTVGITRPCTKHNYLVRDVNDLARVLHEAFYVAQNGRPGPVVVDIPKDVQFAKGTYIGRE
HHCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHCCCEECCC
NIEHKTYKPRLVGDDGAVRQAIEMIAGAKRPIFYTGGGVINSGPAASRALRELARLSGVP
CCCCCCCCCEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCC
VTSTLMGLGAFPAASSQWLGMLGMHGTYEANMAMHGCDVMVCIGARFDDRITGRLDAFAP
HHHHHHHCCCCCCCCCCHHHHHCCCCCEECCEEECCCCEEEEECCCCCCCCCCCEECCCC
GSKKIHIDIDPSSINKNVKVDLPIIGDCTKVLEAMVALWKSQDRQTDKAALDEWWKQIDR
CCCEEEEEECHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
WRARNSLAYRLLPDVIKPQQAIERLYAATKDKDVYITTEVGQHQMWAAQFFRFEEPNRWM
HHHHCCHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCEE
TSGGLGTMGYGLPAAIGAQIAHPDGLCIDIAGEASILMNMQEMSTAVQFNLPVKIFILNN
ECCCCCCCCCCCHHHHCCEECCCCCEEEEECCCHHHEEEHHHHHHEEEECCCEEEEEECC
RYMGMVRQWQELLHGGRYSHSYSEALPDFVKLADAYGGVGIRCEKPGDLDGAIQEMINIK
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHCC
RPVIFDCIVDQQENCFPMIPSGRAHNEMILGDMAVDLDEAITDEGKMLV
CCEEEEEEECCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDDMTKEMTGAEMVIQALTDQGVEHLFGYPGGAVLPIYDALFHQNRIEHILVRHEQAAVH
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAEGYARSSGKVGVVLVTSGPGATNAVTGLTDALMDSIPIVCITGQVATHLIGNDAFQEC
CCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECHHHHHHHCCHHHHHH
DTVGITRPCTKHNYLVRDVNDLARVLHEAFYVAQNGRPGPVVVDIPKDVQFAKGTYIGRE
HHCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHCCCEECCC
NIEHKTYKPRLVGDDGAVRQAIEMIAGAKRPIFYTGGGVINSGPAASRALRELARLSGVP
CCCCCCCCCEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCC
VTSTLMGLGAFPAASSQWLGMLGMHGTYEANMAMHGCDVMVCIGARFDDRITGRLDAFAP
HHHHHHHCCCCCCCCCCHHHHHCCCCCEECCEEECCCCEEEEECCCCCCCCCCCEECCCC
GSKKIHIDIDPSSINKNVKVDLPIIGDCTKVLEAMVALWKSQDRQTDKAALDEWWKQIDR
CCCEEEEEECHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
WRARNSLAYRLLPDVIKPQQAIERLYAATKDKDVYITTEVGQHQMWAAQFFRFEEPNRWM
HHHHCCHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCEE
TSGGLGTMGYGLPAAIGAQIAHPDGLCIDIAGEASILMNMQEMSTAVQFNLPVKIFILNN
ECCCCCCCCCCCHHHHCCEECCCCCEEEEECCCHHHEEEHHHHHHEEEECCCEEEEEECC
RYMGMVRQWQELLHGGRYSHSYSEALPDFVKLADAYGGVGIRCEKPGDLDGAIQEMINIK
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHCC
RPVIFDCIVDQQENCFPMIPSGRAHNEMILGDMAVDLDEAITDEGKMLV
CCEEEEEEECCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]