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The map label for this gene is rhmD

Identifier: 158423895

GI number: 158423895

Start: 2595862

End: 2597043

Strand: Reverse

Name: rhmD

Synonym: AZC_2271

Alternate gene names: 158423895

Gene position: 2597043-2595862 (Counterclockwise)

Preceding gene: 158423896

Following gene: 158423894

Centisome position: 48.36

GC content: 64.97

Gene sequence:

>1182_bases
ATGTCCGTGCCTACCATTCGTGAAGTGCGCGCCTATGTGGTGCGCGGCGGGGGCGGCGATTATCACGACCAGCAGGGCAC
CCACTGGATCGACGACCACATCGCGACCCCCATGTCCAAGTATCCGGCCTATCGCCAGAGCCGGCGCACCTTCGGCATCA
ACGTGCTCGGCACGCTGGTGGTCGAGGTGGAGGCCTCCGACGGCACGGTGGGCTTTGCGGTGACCACTGGCGGCGAGCCC
GCCGCCTGGATCGTCGAGAAGCACCTCGCCCGCTTCATCGAAGGCGCCAAGATCACCGACATCGAGCTGATGTGGGACCA
GATGTACCGCTCGACGCTCTATTACGGGCGCAAGGGTCTGGTGGTGAACACCATCTCGGGCGTGGACCTGGCACTCTGGG
ACCTGCTCGCCAAGTGGCGCAAGGAGCCGGTCTATCACCTGCTCGGCGGCGCGGTGCGCGACGAGCTCCAGTTCTATGCC
ACCGGCGCCCGGCCCGATCTTGCCAAGGAGATGGGCTTCATCGGCGGCAAGATGGCGCTGCACCATGGTCCGGCAGAGGG
CGAGGAGGGGCTTCGCAAGAACCTCGACATCCTCAACGACATGCGGAACAAGGTCGGCAAGGACTTCTGGCTCATGCAGG
ACTGCTGGATGGCGCTGGACGTGGAATATGCCACGCGCTTCTCCACCATCGCCTGGAACGAGATGGGCCTGAAGTGGACC
GAGGAAGCCCTGCCGCCGGATGACTACTGGGGCTATGCGGCGCTCAAGAAGAACGCGCCGAAGGGCCACCTTATCACCAC
CGGCGAGCACGAGGCCACCCGCTGGGGCTTCAAGATGCTGCTGGACATGGAGTGCTGCGACATCATCCAGCCGGACGTGG
GCTGGTGCGGTGGCATCACCGAGCTCATCAAGATCACGGCGCTCGCGGATGCGGCCGGCGTGCTCGTGGTGCCGCACGGC
TCGTCCGTCTACAGCTATCACTTCGTGATCACCCGGCATAACAGCCCGTTTGCCGAGTTTCTGATGATGCACCCGGTGCC
AGATCAGGTGGTGCCCATGTTCACGCCGCTCCTGCTCGACGAGCCTGTGCCGGTGAACGGCCGCCTGAAGCTCGACGACA
CCCCCGGCTTCGGCGTGCGCCTGAACCCCGAGTGCAAGCTCTCGCGCCCCTACACCCACTGA

Upstream 100 bases:

>100_bases
CCGCCACCACCTTCATCGGCGTCCTCGCCATCCTGGCGCTGGGCGCAGCCCGCCAGCCCGTGGCCGGCGCCACCCCTGTG
ACTGCTCGAGGAGACCGTTG

Downstream 100 bases:

>100_bases
GGCGAACGAAATTCCATGAAGCTTCTGCGTTACGGCCCCGCGGGCCAGGAAAAGCCCGGCCTCCTCGATGAGGCCGGCAA
GATCCGCGATCTGTCGGGCG

Product: L-alanine-DL-glutamate epimerase

Products: NA

Alternate protein names: RhamD

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MSVPTIREVRAYVVRGGGGDYHDQQGTHWIDDHIATPMSKYPAYRQSRRTFGINVLGTLVVEVEASDGTVGFAVTTGGEP
AAWIVEKHLARFIEGAKITDIELMWDQMYRSTLYYGRKGLVVNTISGVDLALWDLLAKWRKEPVYHLLGGAVRDELQFYA
TGARPDLAKEMGFIGGKMALHHGPAEGEEGLRKNLDILNDMRNKVGKDFWLMQDCWMALDVEYATRFSTIAWNEMGLKWT
EEALPPDDYWGYAALKKNAPKGHLITTGEHEATRWGFKMLLDMECCDIIQPDVGWCGGITELIKITALADAAGVLVVPHG
SSVYSYHFVITRHNSPFAEFLMMHPVPDQVVPMFTPLLLDEPVPVNGRLKLDDTPGFGVRLNPECKLSRPYTH

Sequences:

>Translated_393_residues
MSVPTIREVRAYVVRGGGGDYHDQQGTHWIDDHIATPMSKYPAYRQSRRTFGINVLGTLVVEVEASDGTVGFAVTTGGEP
AAWIVEKHLARFIEGAKITDIELMWDQMYRSTLYYGRKGLVVNTISGVDLALWDLLAKWRKEPVYHLLGGAVRDELQFYA
TGARPDLAKEMGFIGGKMALHHGPAEGEEGLRKNLDILNDMRNKVGKDFWLMQDCWMALDVEYATRFSTIAWNEMGLKWT
EEALPPDDYWGYAALKKNAPKGHLITTGEHEATRWGFKMLLDMECCDIIQPDVGWCGGITELIKITALADAAGVLVVPHG
SSVYSYHFVITRHNSPFAEFLMMHPVPDQVVPMFTPLLLDEPVPVNGRLKLDDTPGFGVRLNPECKLSRPYTH
>Mature_392_residues
SVPTIREVRAYVVRGGGGDYHDQQGTHWIDDHIATPMSKYPAYRQSRRTFGINVLGTLVVEVEASDGTVGFAVTTGGEPA
AWIVEKHLARFIEGAKITDIELMWDQMYRSTLYYGRKGLVVNTISGVDLALWDLLAKWRKEPVYHLLGGAVRDELQFYAT
GARPDLAKEMGFIGGKMALHHGPAEGEEGLRKNLDILNDMRNKVGKDFWLMQDCWMALDVEYATRFSTIAWNEMGLKWTE
EALPPDDYWGYAALKKNAPKGHLITTGEHEATRWGFKMLLDMECCDIIQPDVGWCGGITELIKITALADAAGVLVVPHGS
SVYSYHFVITRHNSPFAEFLMMHPVPDQVVPMFTPLLLDEPVPVNGRLKLDDTPGFGVRLNPECKLSRPYTH

Specific function: Catalyzes the dehydration of L-rhamnonate to 2-keto-3- deoxy-L-rhamnonate (KDR)

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily

Homologues:

Organism=Escherichia coli, GI226510960, Length=403, Percent_Identity=60.7940446650124, Blast_Score=486, Evalue=1e-139,
Organism=Escherichia coli, GI1787864, Length=380, Percent_Identity=25.5263157894737, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RHMD_AZOC5 (A8I5F1)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001525187.1
- ProteinModelPortal:   A8I5F1
- SMR:   A8I5F1
- GeneID:   5689783
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_2271
- HOGENOM:   HBG705576
- OMA:   DEVVPMF
- ProtClustDB:   PRK15440
- BioCyc:   ACAU438753:AZC_2271-MONOMER
- HAMAP:   MF_01288
- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354
- PANTHER:   PTHR13794

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N

EC number: =4.2.1.90

Molecular weight: Translated: 44053; Mature: 43922

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00908 MR_MLE_1; PS00909 MR_MLE_2

Important sites: ACT_SITE 319-319 BINDING 22-22 BINDING 48-48 BINDING 339-339

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPTIREVRAYVVRGGGGDYHDQQGTHWIDDHIATPMSKYPAYRQSRRTFGINVLGTLV
CCCCCHHHEEEEEEECCCCCCCCCCCCEEHHHHHCCCHHHCCHHHHHCCEECEEEEEEEE
VEVEASDGTVGFAVTTGGEPAAWIVEKHLARFIEGAKITDIELMWDQMYRSTLYYGRKGL
EEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCE
VVNTISGVDLALWDLLAKWRKEPVYHLLGGAVRDELQFYATGARPDLAKEMGFIGGKMAL
EEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHCCCCEEEE
HHGPAEGEEGLRKNLDILNDMRNKVGKDFWLMQDCWMALDVEYATRFSTIAWNEMGLKWT
ECCCCCCHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHEEEHHHHHHEEEEECCCCCEEH
EEALPPDDYWGYAALKKNAPKGHLITTGEHEATRWGFKMLLDMECCDIIQPDVGWCGGIT
HHCCCCCCCCCHHHEECCCCCCCEEECCCCCHHHHHHHHEECCHHHHHCCCCCCCCCCHH
ELIKITALADAAGVLVVPHGSSVYSYHFVITRHNSPFAEFLMMHPVPDQVVPMFTPLLLD
HHHHHHHEECCCCEEEEECCCCEEEEEEEEEECCCCHHHHHEECCCCCHHHHHHHHHHHC
EPVPVNGRLKLDDTPGFGVRLNPECKLSRPYTH
CCCCCCCEEEECCCCCCCEEECCCCCCCCCCCC
>Mature Secondary Structure 
SVPTIREVRAYVVRGGGGDYHDQQGTHWIDDHIATPMSKYPAYRQSRRTFGINVLGTLV
CCCCHHHEEEEEEECCCCCCCCCCCCEEHHHHHCCCHHHCCHHHHHCCEECEEEEEEEE
VEVEASDGTVGFAVTTGGEPAAWIVEKHLARFIEGAKITDIELMWDQMYRSTLYYGRKGL
EEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCE
VVNTISGVDLALWDLLAKWRKEPVYHLLGGAVRDELQFYATGARPDLAKEMGFIGGKMAL
EEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHCCCCEEEE
HHGPAEGEEGLRKNLDILNDMRNKVGKDFWLMQDCWMALDVEYATRFSTIAWNEMGLKWT
ECCCCCCHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHEEEHHHHHHEEEEECCCCCEEH
EEALPPDDYWGYAALKKNAPKGHLITTGEHEATRWGFKMLLDMECCDIIQPDVGWCGGIT
HHCCCCCCCCCHHHEECCCCCCCEEECCCCCHHHHHHHHEECCHHHHHCCCCCCCCCCHH
ELIKITALADAAGVLVVPHGSSVYSYHFVITRHNSPFAEFLMMHPVPDQVVPMFTPLLLD
HHHHHHHEECCCCEEEEECCCCEEEEEEEEEECCCCHHHHHEECCCCCHHHHHHHHHHHC
EPVPVNGRLKLDDTPGFGVRLNPECKLSRPYTH
CCCCCCCEEEECCCCCCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA