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The map label for this gene is purB [H]

Identifier: 158423861

GI number: 158423861

Start: 2555179

End: 2556486

Strand: Reverse

Name: purB [H]

Synonym: AZC_2237

Alternate gene names: 158423861

Gene position: 2556486-2555179 (Counterclockwise)

Preceding gene: 158423862

Following gene: 158423860

Centisome position: 47.61

GC content: 66.28

Gene sequence:

>1308_bases
ATGATCCCCCGCTACAGCCGGCCCCAGATGGCCGACATCTGGTCCCCCGAAACCCGATTCCGCATCTGGTTCGAGATCGA
GGCCCATGCCGCCGATGCCATGGCCGAACTCGGCATTGTGCCGAAGGAAGCGGCAAAGGTGATCTGGGAGAAGGGCGCCA
ACGCCACCTTCGACGTGGAGCGCATCGACGCCATCGAGCGCGAAGTGAAGCACGACGTCATCGCCTTCCTCACGCATCTG
GCCGAGATCGTCGGCCCCGACGCGCGCTTCGTCCATCAGGGCATGACCTCGTCCGACGTGCTGGACACGTGCCTTGCCGT
TCAGCTCACCCGCGCCGCCGATCTCCTGATCGCGGACGTGGACAATCTGCTGGCGATCCTCGAGCGCCGTGCCTTCGAGC
ACAAGCTGACCCCCACCGTCGGCCGCTCCCACGGCATCCATGCCGAGCCCACCACCTTCGGCGTCAAGCTCGCCATTGCG
CACGCCGAATTCCAGCGCTCCCGCGCCCGCCTCGTCGCCGCCCGCGCTGAGATCGCCACCTGCGCCATCTCGGGCGCGGT
GGGCACCTTCGCCCATATCGATCCGCGCGTGGAGCAGCATGTGGCGGAGAAGATGGGCCTGACGGTCGAGACCGTCTCCA
CGCAGGTGATCCCGCGCGACCGCCACGCCATGTTCTTTGCCGTGCTCGGCGTCGTCGCCTCCTCCGTGGAGCGGCTCGCC
ACCGAGGTACGCCACCTCCAGCGCACCGAGGTGCTGGAAGCGGAAGAGTATTTCTCCGAGGCCCAGAAGGGCTCCTCGGC
CATGCCGCACAAGCGCAATCCGGTGCTGACCGAGAACCTCACGGGCCTTGCCCGCATGGTGCGCGGCTATGTGACCCCGG
CGCTGGAGAATGTGGCGCTTTGGCACGAGCGCGACATCTCGCACTCCTCCGTGGAGCGCATGATCGGCCCCGACGCCACC
GTGACGCTGGACTTCGCCCTCGCCCGTCTCGCCGGCGTGATGGACAAGCTCGTCGTCCATGCCGACAACATGCAGAAGAA
CATGGACAAGCTGGGTGGCCTCATCCACTCCCAGCGCGTGCTGCTGGCGCTGACCCAGAAGGGCGCGAGCCGCGAGGACA
GCTATCGCCTCGTGCAGCGCAACGCCATGAAGGTCTGGCGCGGCGAAGGCGACTTCCGCGCCTTCCTCTTGGCGGACGAA
GAGGTGCGCAAGTATCTCTCCGAGGACGACATCAACGAGAAGTTCGATCTCGGCTACCACTTCAAGAACGTGGACCAGAT
CTTCACCCGCGTCTTCGGCCGCGCCTGA

Upstream 100 bases:

>100_bases
ATGATCTCAGCGCAACGCTCGCGTTCCGGCGCGCGCCTATCCAGCAGCGCCGCGATCTGATAGAGCGCCCGCTTCAACGA
CAAGGCGTGCGAGCACCGCG

Downstream 100 bases:

>100_bases
GATTCTGCTTTTAATCTGAGAATTGGGAATGCCCGGCTTTGGCCGGGCATTTTTGTTTGGCGGACTTGATTGCCTCTCAA
ATGAGATACACATTACGTCC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MIPRYSRPQMADIWSPETRFRIWFEIEAHAADAMAELGIVPKEAAKVIWEKGANATFDVERIDAIEREVKHDVIAFLTHL
AEIVGPDARFVHQGMTSSDVLDTCLAVQLTRAADLLIADVDNLLAILERRAFEHKLTPTVGRSHGIHAEPTTFGVKLAIA
HAEFQRSRARLVAARAEIATCAISGAVGTFAHIDPRVEQHVAEKMGLTVETVSTQVIPRDRHAMFFAVLGVVASSVERLA
TEVRHLQRTEVLEAEEYFSEAQKGSSAMPHKRNPVLTENLTGLARMVRGYVTPALENVALWHERDISHSSVERMIGPDAT
VTLDFALARLAGVMDKLVVHADNMQKNMDKLGGLIHSQRVLLALTQKGASREDSYRLVQRNAMKVWRGEGDFRAFLLADE
EVRKYLSEDDINEKFDLGYHFKNVDQIFTRVFGRA

Sequences:

>Translated_435_residues
MIPRYSRPQMADIWSPETRFRIWFEIEAHAADAMAELGIVPKEAAKVIWEKGANATFDVERIDAIEREVKHDVIAFLTHL
AEIVGPDARFVHQGMTSSDVLDTCLAVQLTRAADLLIADVDNLLAILERRAFEHKLTPTVGRSHGIHAEPTTFGVKLAIA
HAEFQRSRARLVAARAEIATCAISGAVGTFAHIDPRVEQHVAEKMGLTVETVSTQVIPRDRHAMFFAVLGVVASSVERLA
TEVRHLQRTEVLEAEEYFSEAQKGSSAMPHKRNPVLTENLTGLARMVRGYVTPALENVALWHERDISHSSVERMIGPDAT
VTLDFALARLAGVMDKLVVHADNMQKNMDKLGGLIHSQRVLLALTQKGASREDSYRLVQRNAMKVWRGEGDFRAFLLADE
EVRKYLSEDDINEKFDLGYHFKNVDQIFTRVFGRA
>Mature_435_residues
MIPRYSRPQMADIWSPETRFRIWFEIEAHAADAMAELGIVPKEAAKVIWEKGANATFDVERIDAIEREVKHDVIAFLTHL
AEIVGPDARFVHQGMTSSDVLDTCLAVQLTRAADLLIADVDNLLAILERRAFEHKLTPTVGRSHGIHAEPTTFGVKLAIA
HAEFQRSRARLVAARAEIATCAISGAVGTFAHIDPRVEQHVAEKMGLTVETVSTQVIPRDRHAMFFAVLGVVASSVERLA
TEVRHLQRTEVLEAEEYFSEAQKGSSAMPHKRNPVLTENLTGLARMVRGYVTPALENVALWHERDISHSSVERMIGPDAT
VTLDFALARLAGVMDKLVVHADNMQKNMDKLGGLIHSQRVLLALTQKGASREDSYRLVQRNAMKVWRGEGDFRAFLLADE
EVRKYLSEDDINEKFDLGYHFKNVDQIFTRVFGRA

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=402, Percent_Identity=27.6119402985075, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI183227688, Length=381, Percent_Identity=27.5590551181102, Blast_Score=131, Evalue=1e-30,
Organism=Escherichia coli, GI1787376, Length=351, Percent_Identity=29.3447293447293, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17508577, Length=395, Percent_Identity=25.3164556962025, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32564234, Length=358, Percent_Identity=25.9776536312849, Blast_Score=88, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6323391, Length=404, Percent_Identity=27.7227722772277, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24647570, Length=381, Percent_Identity=25.4593175853018, Blast_Score=102, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48674; Mature: 48674

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYSRPQMADIWSPETRFRIWFEIEAHAADAMAELGIVPKEAAKVIWEKGANATFDVE
CCCCCCCCCCCCCCCCCCEEEEEEEEECHHHHHHHHHCCCCHHHHHHHHHCCCCCEECHH
RIDAIEREVKHDVIAFLTHLAEIVGPDARFVHQGMTSSDVLDTCLAVQLTRAADLLIADV
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DNLLAILERRAFEHKLTPTVGRSHGIHAEPTTFGVKLAIAHAEFQRSRARLVAARAEIAT
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
CAISGAVGTFAHIDPRVEQHVAEKMGLTVETVSTQVIPRDRHAMFFAVLGVVASSVERLA
HHHHCCHHHHHHCCHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
TEVRHLQRTEVLEAEEYFSEAQKGSSAMPHKRNPVLTENLTGLARMVRGYVTPALENVAL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WHERDISHSSVERMIGPDATVTLDFALARLAGVMDKLVVHADNMQKNMDKLGGLIHSQRV
HHHCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLALTQKGASREDSYRLVQRNAMKVWRGEGDFRAFLLADEEVRKYLSEDDINEKFDLGYH
HHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHCCCCCCCHHCCCCC
FKNVDQIFTRVFGRA
CCCHHHHHHHHHCCC
>Mature Secondary Structure
MIPRYSRPQMADIWSPETRFRIWFEIEAHAADAMAELGIVPKEAAKVIWEKGANATFDVE
CCCCCCCCCCCCCCCCCCEEEEEEEEECHHHHHHHHHCCCCHHHHHHHHHCCCCCEECHH
RIDAIEREVKHDVIAFLTHLAEIVGPDARFVHQGMTSSDVLDTCLAVQLTRAADLLIADV
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DNLLAILERRAFEHKLTPTVGRSHGIHAEPTTFGVKLAIAHAEFQRSRARLVAARAEIAT
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
CAISGAVGTFAHIDPRVEQHVAEKMGLTVETVSTQVIPRDRHAMFFAVLGVVASSVERLA
HHHHCCHHHHHHCCHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
TEVRHLQRTEVLEAEEYFSEAQKGSSAMPHKRNPVLTENLTGLARMVRGYVTPALENVAL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WHERDISHSSVERMIGPDATVTLDFALARLAGVMDKLVVHADNMQKNMDKLGGLIHSQRV
HHHCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLALTQKGASREDSYRLVQRNAMKVWRGEGDFRAFLLADEEVRKYLSEDDINEKFDLGYH
HHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHCCCCCCCHHCCCCC
FKNVDQIFTRVFGRA
CCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]