Click here to switch to the map view.

The map label for this gene is echA8 [H]

Identifier: 158423811

GI number: 158423811

Start: 2503324

End: 2504148

Strand: Reverse

Name: echA8 [H]

Synonym: AZC_2187

Alternate gene names: 158423811

Gene position: 2504148-2503324 (Counterclockwise)

Preceding gene: 158423817

Following gene: 158423810

Centisome position: 46.63

GC content: 66.06

Gene sequence:

>825_bases
ATGAGCGAAGCGTCTGCATCCGAAATGGAAGCTTCCTGGGGATTTATCCGACTACAGCGCGCATCCGGCGTTGCGATCCT
CACCCTCGCCCGGCCGGCCAGCCGCAACAGCCTCTCCGACGGCATGATGGCCGATGTGTCTGGCGCGCTGACAGAATTGG
CCGATGACGCCAGCGTTCGTGCCATTGTCCTGGACGCCGAAGGCCCGGTCTTCTCCGCCGGCCACGACCTGAAGGAAATT
CAGGCGCATCGCAGCGACAACGATCGCGGTCGTACCTATTTCGAATCTGTTCTGGCCCGTTGCTCGGCGCTGATGACCCA
GATCGTCCGCCAACCCCAGCCGGTGATCGCCGCCGTGGAGGGCATGGCGACCGCCGCCGGCTGCCAACTGGTCGCGAGCT
GCGACCTCGCGGTGGCCGGGGCCGGCGCCCGCTTCTGCACGCCGGGCGTGAACATCGGCCTCTTCTGCCACACGCCCATG
GTGGCGCTGTCCCGCTCCGTCGGGCGCAAGCACGCCATGGAGATGCTGTTGCTCGGCGAATGGGTCGAGGCGCCCGAGGC
CCATCGTATGGGCCTCGTCAACCGAATCGCTCCGGCCGGCGAGGCGCGATCCTTGGCGCTGGAGCTCGCGCAGAAGATCG
CCTCGAAATCGCGGGTCCCGGTGAAACTCGGAAAGGCCGCCTTCTATACGCAGGCCGAGATGGGCCTTGATGACGCCTAT
GCCTACGCGTCCCGCGTCATGGCGGAGAACATGATGGCACGCGATGCGGACGAGGGCATTTCGGCGGTGATCGGCAAGCG
CACACCGCAATGGGAAGACCGCTGA

Upstream 100 bases:

>100_bases
TTCTTCCACTCCCTGTGTAGCTTACATATGACACGAGCAAGCTTGTTTGAAACCTGCATAAAGCGCAGCAATACCCCTAC
ATAATACCGGGAGGACGGAA

Downstream 100 bases:

>100_bases
GCGACAATTTACCCTTCATCAACCATGCAGGCGCACCCTGAAATCGGACGTCTCCCGCACTCTGGCGGGGACGCAGGAGC
GCATGCATGGCCCTCGCCTG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSEASASEMEASWGFIRLQRASGVAILTLARPASRNSLSDGMMADVSGALTELADDASVRAIVLDAEGPVFSAGHDLKEI
QAHRSDNDRGRTYFESVLARCSALMTQIVRQPQPVIAAVEGMATAAGCQLVASCDLAVAGAGARFCTPGVNIGLFCHTPM
VALSRSVGRKHAMEMLLLGEWVEAPEAHRMGLVNRIAPAGEARSLALELAQKIASKSRVPVKLGKAAFYTQAEMGLDDAY
AYASRVMAENMMARDADEGISAVIGKRTPQWEDR

Sequences:

>Translated_274_residues
MSEASASEMEASWGFIRLQRASGVAILTLARPASRNSLSDGMMADVSGALTELADDASVRAIVLDAEGPVFSAGHDLKEI
QAHRSDNDRGRTYFESVLARCSALMTQIVRQPQPVIAAVEGMATAAGCQLVASCDLAVAGAGARFCTPGVNIGLFCHTPM
VALSRSVGRKHAMEMLLLGEWVEAPEAHRMGLVNRIAPAGEARSLALELAQKIASKSRVPVKLGKAAFYTQAEMGLDDAY
AYASRVMAENMMARDADEGISAVIGKRTPQWEDR
>Mature_273_residues
SEASASEMEASWGFIRLQRASGVAILTLARPASRNSLSDGMMADVSGALTELADDASVRAIVLDAEGPVFSAGHDLKEIQ
AHRSDNDRGRTYFESVLARCSALMTQIVRQPQPVIAAVEGMATAAGCQLVASCDLAVAGAGARFCTPGVNIGLFCHTPMV
ALSRSVGRKHAMEMLLLGEWVEAPEAHRMGLVNRIAPAGEARSLALELAQKIASKSRVPVKLGKAAFYTQAEMGLDDAYA
YASRVMAENMMARDADEGISAVIGKRTPQWEDR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=249, Percent_Identity=47.3895582329317, Blast_Score=231, Evalue=6e-61,
Organism=Homo sapiens, GI194097323, Length=256, Percent_Identity=32.421875, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=27.34375, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI70995211, Length=224, Percent_Identity=29.0178571428571, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI62530384, Length=191, Percent_Identity=27.7486910994764, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI68989263, Length=204, Percent_Identity=27.9411764705882, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI221136756, Length=251, Percent_Identity=27.4900398406374, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI221136753, Length=251, Percent_Identity=27.4900398406374, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI221307494, Length=251, Percent_Identity=27.4900398406374, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=33.5907335907336, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI221142681, Length=266, Percent_Identity=29.3233082706767, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1787660, Length=263, Percent_Identity=30.4182509505703, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082183, Length=265, Percent_Identity=23.0188679245283, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788597, Length=278, Percent_Identity=26.978417266187, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1788682, Length=280, Percent_Identity=25.7142857142857, Blast_Score=68, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=237, Percent_Identity=43.8818565400844, Blast_Score=189, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17554946, Length=259, Percent_Identity=33.5907335907336, Blast_Score=137, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI25145438, Length=255, Percent_Identity=30.9803921568627, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17560910, Length=248, Percent_Identity=27.0161290322581, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17540714, Length=235, Percent_Identity=30.6382978723404, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=260, Percent_Identity=26.5384615384615, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=218, Percent_Identity=27.0642201834862, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19922422, Length=260, Percent_Identity=36.5384615384615, Blast_Score=174, Evalue=5e-44,
Organism=Drosophila melanogaster, GI20129971, Length=273, Percent_Identity=31.8681318681319, Blast_Score=147, Evalue=8e-36,
Organism=Drosophila melanogaster, GI24653477, Length=273, Percent_Identity=31.8681318681319, Blast_Score=147, Evalue=8e-36,
Organism=Drosophila melanogaster, GI24653139, Length=258, Percent_Identity=27.5193798449612, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19920382, Length=222, Percent_Identity=31.981981981982, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24654903, Length=183, Percent_Identity=28.9617486338798, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21357171, Length=250, Percent_Identity=26.4, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24650670, Length=266, Percent_Identity=26.3157894736842, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 29153; Mature: 29022

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEASASEMEASWGFIRLQRASGVAILTLARPASRNSLSDGMMADVSGALTELADDASVR
CCCCCCHHHHHCCCEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEE
AIVLDAEGPVFSAGHDLKEIQAHRSDNDRGRTYFESVLARCSALMTQIVRQPQPVIAAVE
EEEEECCCCEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
GMATAAGCQLVASCDLAVAGAGARFCTPGVNIGLFCHTPMVALSRSVGRKHAMEMLLLGE
HHHHHHHHHHHHHCCEEEECCCCCEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
WVEAPEAHRMGLVNRIAPAGEARSLALELAQKIASKSRVPVKLGKAAFYTQAEMGLDDAY
HHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHCCCHHHH
AYASRVMAENMMARDADEGISAVIGKRTPQWEDR
HHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SEASASEMEASWGFIRLQRASGVAILTLARPASRNSLSDGMMADVSGALTELADDASVR
CCCCCHHHHHCCCEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEE
AIVLDAEGPVFSAGHDLKEIQAHRSDNDRGRTYFESVLARCSALMTQIVRQPQPVIAAVE
EEEEECCCCEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
GMATAAGCQLVASCDLAVAGAGARFCTPGVNIGLFCHTPMVALSRSVGRKHAMEMLLLGE
HHHHHHHHHHHHHCCEEEECCCCCEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
WVEAPEAHRMGLVNRIAPAGEARSLALELAQKIASKSRVPVKLGKAAFYTQAEMGLDDAY
HHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHCCCHHHH
AYASRVMAENMMARDADEGISAVIGKRTPQWEDR
HHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]