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The map label for this gene is potH [H]

Identifier: 158423614

GI number: 158423614

Start: 2293965

End: 2294801

Strand: Reverse

Name: potH [H]

Synonym: AZC_1990

Alternate gene names: 158423614

Gene position: 2294801-2293965 (Counterclockwise)

Preceding gene: 158423615

Following gene: 158423613

Centisome position: 42.74

GC content: 67.98

Gene sequence:

>837_bases
ATGACGCGCCTTGGCAAACTTGATCCCGTGACGCTGCTGGCGCTGCCCGCCGTGGCCTATCTCGGCCTCGTCTATGGCCT
GCCGCTGGTCGGCCTGCTGGCCCGTTCGCTGATGGTGGAGGGCACGGTCTCCCTCACCTCCTACACCCGCTTCCTGTCCG
ATCCCTTCAGTTGGACGGTGATCGGCAACACGCTGCGGGTGGCGGCCGGCGTGACCTTGCTGTGCCTCTTCATCGGCTAC
CCGACCGCGCTGGCGCTCGCCCGCGCCAAGGGGGCGGCGCAGGCGCTCATTCTGGTGGCCATCATCCTTCCCCTCTCCGT
GGGTGTGGTGGTGAAGGCCTTCGCCTGGCAGATCGTGCTGCGGCGGGACGGGGTCATCTCGCAGCTTCTCGTTGGCCTTG
GCCTGTGGGACGCGCCGCAGCGCCTGCTGTTCACCGAGACGGGGCTGGTGGTGGGCGCGGCGAACGTGTTCGTGCCCTTC
ATGATCCTGCCCATCTATTCGGTGCTGAAGCTGGTGGACCCGCGCCTCATCGAGGCCGCCGCGACGCTCGGCGCCTCGCC
GCTCTATCGCTTCTTCCACGTGGCCCTGCCGCTCTCCCTGCCGGGCATCGTGACGGGCATCGCCTTCGTCTTCTCCATGG
CCACCTCCATGTATGTGGTGCCGAGCCTGCTCATCGGCGACCGCTTCCAGACGCTGGCGACCCTGACCGGCCGCTCCTTC
CTCTTCATGCGCAACGAGCAACTGGGCTCCACCACAGCCGTCGTGCTGCTGATCCTCTCGGTGGCCGTGGTGGTGGCGAG
CGCGGCGCTCTCCCGCCGGTTTGGAGGCGCGTCATGA

Upstream 100 bases:

>100_bases
AAGGCGCCGAACCGGGCGTGCCGTGCGCGCTGTCCTTCGCGGTGGAGGACACGCTGGTCTATCCCGCGCCGGAGCTGGAC
CACGCGGTCGAGGAGGCGGC

Downstream 100 bases:

>100_bases
CCCTCACCGAGCGCATCGCCTCTGTCCTCGTCTGGGTCATCGCGGCCTTCACGGTCATTTTCCTGCTGACGCCGCTGGTG
GTGACGGTGGCCGTCTCCTT

Product: ABC transporter permease

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MTRLGKLDPVTLLALPAVAYLGLVYGLPLVGLLARSLMVEGTVSLTSYTRFLSDPFSWTVIGNTLRVAAGVTLLCLFIGY
PTALALARAKGAAQALILVAIILPLSVGVVVKAFAWQIVLRRDGVISQLLVGLGLWDAPQRLLFTETGLVVGAANVFVPF
MILPIYSVLKLVDPRLIEAAATLGASPLYRFFHVALPLSLPGIVTGIAFVFSMATSMYVVPSLLIGDRFQTLATLTGRSF
LFMRNEQLGSTTAVVLLILSVAVVVASAALSRRFGGAS

Sequences:

>Translated_278_residues
MTRLGKLDPVTLLALPAVAYLGLVYGLPLVGLLARSLMVEGTVSLTSYTRFLSDPFSWTVIGNTLRVAAGVTLLCLFIGY
PTALALARAKGAAQALILVAIILPLSVGVVVKAFAWQIVLRRDGVISQLLVGLGLWDAPQRLLFTETGLVVGAANVFVPF
MILPIYSVLKLVDPRLIEAAATLGASPLYRFFHVALPLSLPGIVTGIAFVFSMATSMYVVPSLLIGDRFQTLATLTGRSF
LFMRNEQLGSTTAVVLLILSVAVVVASAALSRRFGGAS
>Mature_277_residues
TRLGKLDPVTLLALPAVAYLGLVYGLPLVGLLARSLMVEGTVSLTSYTRFLSDPFSWTVIGNTLRVAAGVTLLCLFIGYP
TALALARAKGAAQALILVAIILPLSVGVVVKAFAWQIVLRRDGVISQLLVGLGLWDAPQRLLFTETGLVVGAANVFVPFM
ILPIYSVLKLVDPRLIEAAATLGASPLYRFFHVALPLSLPGIVTGIAFVFSMATSMYVVPSLLIGDRFQTLATLTGRSFL
FMRNEQLGSTTAVVLLILSVAVVVASAALSRRFGGAS

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=218, Percent_Identity=35.3211009174312, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1787369, Length=220, Percent_Identity=32.7272727272727, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1787713, Length=225, Percent_Identity=32.8888888888889, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29447; Mature: 29316

Theoretical pI: Translated: 10.59; Mature: 10.59

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TRLGKLDPVTLLALPAVAYLGLVYGLPLVGLLARSLMVEGTVSLTSYTRFLSDPFSWTV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCEEEE
IGNTLRVAAGVTLLCLFIGYPTALALARAKGAAQALILVAIILPLSVGVVVKAFAWQIVL
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRDGVISQLLVGLGLWDAPQRLLFTETGLVVGAANVFVPFMILPIYSVLKLVDPRLIEAA
HCCCHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
ATLGASPLYRFFHVALPLSLPGIVTGIAFVFSMATSMYVVPSLLIGDRFQTLATLTGRSF
HHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LFMRNEQLGSTTAVVLLILSVAVVVASAALSRRFGGAS
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]