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The map label for this gene is lysR [H]

Identifier: 158423573

GI number: 158423573

Start: 2250261

End: 2251217

Strand: Reverse

Name: lysR [H]

Synonym: AZC_1949

Alternate gene names: 158423573

Gene position: 2251217-2250261 (Counterclockwise)

Preceding gene: 158423574

Following gene: 158423571

Centisome position: 41.92

GC content: 67.5

Gene sequence:

>957_bases
ATGCCGCCATCCCGCCACTGGACGCACGGGCTCGACTGGAACCTGCTGCGCACGTTCCTCGTGATCGCCGAAGAGCGGAG
CGTAACGCGCGCCGCCGAGCGCCTGCTGCTCCAGCAGCCGAGCGTCAGCGCCGCTTTGAAGCGGTTGGAAGAGACCTGCG
AGTGCCGGCTCTTCGAGCGGGGCTGCCGGCCCATGCGTCTCACGCCGCAGGGCGAGGCGCTCTACCAGCAGTGCCGCGAA
CTCTATCGCGCCATCCTCACTCTCGATCCCGGCATCGCCCCCTCTTCCAGCAGCCTGACCGGGCTGGTGCGGATCGAGAT
CGTGACCCATCTGGTGAATAGCGGGCTTGATGCGGTTGTGCGGCGGCTCAACGCCAAGCACCCCGGCATTTCCTACAATG
TGGAAGTGTCCAGCAGCTACGGCATCGTGCGGGCCGTCTCGCAGCAGCTCACGCCCTTCGGCATCTGCCTTCTGGCCAAA
CCCCTTCCCACGCTCTCTTGCCGGTTCCTGTTCCGCGAGCGGTTCGGCGTCTTCTGTGGGCGCGGCCATCGCCTGTTCGG
CGTGGAAGCGATCGACTTCGACGCCATCCGCAACGAACCCTGGGTCTCGTTCGCCTGCGCCGAGGTGTCCAACTCGCTGG
AGCCGATGCTGACCCTGCGCCTCGGCGCCGATATCGCCCGCCGGGTGGTGGCGACGAGCCCCGACCTGTTCGAGGTCAGG
CGCTTCGTCAACGCTGGCGTCGGCATCGGCACTCTGCCGCTGGAAGCGGCGGCGCGGGATGTGGCGGAAGGCGCACTGTG
GCCCCTCCCCCTCCCCGGCCTCGATGCGCTGGGCGCCGACGTCTATTTTCTCTCCAATCCGGCGCTCCGCTACAGTCCCG
CGGAGCAGGCCTTCATGGAGGAAATGTCGGCGTTCGAGGATGCGAACGCCGACATAGAGGCACCGGTCGGGCCGTGA

Upstream 100 bases:

>100_bases
CGGAGGACCGGTGCCCTTCCCCAGTGGATCGGGCCAGCAACCGGGCTGGCCCTTACGCATCCGGCAGGGGTAGGATGGAC
AGGCATCACAAGGGCCGAAA

Downstream 100 bases:

>100_bases
GTGATCGCGGGAGCCGGCCGCTCCCGCCCGCACGTCAGTGCGCTTCGGCGAGATCGGCCTTCAGCGTCTCCACCAGCATC
AGCAGCGCGATGGTCGAGAT

Product: transcriptional regulator

Products: NA

Alternate protein names: Citrate synthase I repressor [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MPPSRHWTHGLDWNLLRTFLVIAEERSVTRAAERLLLQQPSVSAALKRLEETCECRLFERGCRPMRLTPQGEALYQQCRE
LYRAILTLDPGIAPSSSSLTGLVRIEIVTHLVNSGLDAVVRRLNAKHPGISYNVEVSSSYGIVRAVSQQLTPFGICLLAK
PLPTLSCRFLFRERFGVFCGRGHRLFGVEAIDFDAIRNEPWVSFACAEVSNSLEPMLTLRLGADIARRVVATSPDLFEVR
RFVNAGVGIGTLPLEAAARDVAEGALWPLPLPGLDALGADVYFLSNPALRYSPAEQAFMEEMSAFEDANADIEAPVGP

Sequences:

>Translated_318_residues
MPPSRHWTHGLDWNLLRTFLVIAEERSVTRAAERLLLQQPSVSAALKRLEETCECRLFERGCRPMRLTPQGEALYQQCRE
LYRAILTLDPGIAPSSSSLTGLVRIEIVTHLVNSGLDAVVRRLNAKHPGISYNVEVSSSYGIVRAVSQQLTPFGICLLAK
PLPTLSCRFLFRERFGVFCGRGHRLFGVEAIDFDAIRNEPWVSFACAEVSNSLEPMLTLRLGADIARRVVATSPDLFEVR
RFVNAGVGIGTLPLEAAARDVAEGALWPLPLPGLDALGADVYFLSNPALRYSPAEQAFMEEMSAFEDANADIEAPVGP
>Mature_317_residues
PPSRHWTHGLDWNLLRTFLVIAEERSVTRAAERLLLQQPSVSAALKRLEETCECRLFERGCRPMRLTPQGEALYQQCREL
YRAILTLDPGIAPSSSSLTGLVRIEIVTHLVNSGLDAVVRRLNAKHPGISYNVEVSSSYGIVRAVSQQLTPFGICLLAKP
LPTLSCRFLFRERFGVFCGRGHRLFGVEAIDFDAIRNEPWVSFACAEVSNSLEPMLTLRLGADIARRVVATSPDLFEVRR
FVNAGVGIGTLPLEAAARDVAEGALWPLPLPGLDALGADVYFLSNPALRYSPAEQAFMEEMSAFEDANADIEAPVGP

Specific function: Negative regulatory protein for the citA gene for citrate synthase I [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788748, Length=244, Percent_Identity=27.0491803278689, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI145693105, Length=245, Percent_Identity=24.4897959183673, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787589, Length=135, Percent_Identity=31.1111111111111, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787601, Length=179, Percent_Identity=24.0223463687151, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35034; Mature: 34903

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPSRHWTHGLDWNLLRTFLVIAEERSVTRAAERLLLQQPSVSAALKRLEETCECRLFER
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
GCRPMRLTPQGEALYQQCRELYRAILTLDPGIAPSSSSLTGLVRIEIVTHLVNSGLDAVV
CCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHH
RRLNAKHPGISYNVEVSSSYGIVRAVSQQLTPFGICLLAKPLPTLSCRFLFRERFGVFCG
HHHCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
RGHRLFGVEAIDFDAIRNEPWVSFACAEVSNSLEPMLTLRLGADIARRVVATSPDLFEVR
CCCEEEEEEEECHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
RFVNAGVGIGTLPLEAAARDVAEGALWPLPLPGLDALGADVYFLSNPALRYSPAEQAFME
HHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHCCCEEEECCCCCCCCCHHHHHHH
EMSAFEDANADIEAPVGP
HHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PPSRHWTHGLDWNLLRTFLVIAEERSVTRAAERLLLQQPSVSAALKRLEETCECRLFER
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
GCRPMRLTPQGEALYQQCRELYRAILTLDPGIAPSSSSLTGLVRIEIVTHLVNSGLDAVV
CCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHH
RRLNAKHPGISYNVEVSSSYGIVRAVSQQLTPFGICLLAKPLPTLSCRFLFRERFGVFCG
HHHCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
RGHRLFGVEAIDFDAIRNEPWVSFACAEVSNSLEPMLTLRLGADIARRVVATSPDLFEVR
CCCEEEEEEEECHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
RFVNAGVGIGTLPLEAAARDVAEGALWPLPLPGLDALGADVYFLSNPALRYSPAEQAFME
HHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHCCCEEEECCCCCCCCCHHHHHHH
EMSAFEDANADIEAPVGP
HHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8045898; 9579061; 9384377; 8045899 [H]