Click here to switch to the map view.

The map label for this gene is gloA [H]

Identifier: 158423558

GI number: 158423558

Start: 2236122

End: 2236544

Strand: Reverse

Name: gloA [H]

Synonym: AZC_1934

Alternate gene names: 158423558

Gene position: 2236544-2236122 (Counterclockwise)

Preceding gene: 158423562

Following gene: 158423557

Centisome position: 41.65

GC content: 67.14

Gene sequence:

>423_bases
ATGCGCTATCTCCACACCATGGTCCGCGTCACGGATCTCGACGCCTCGCTGGATTTCTACTGCAACAAGCTGGGGCTCGT
CGAAGTCCGCCGCAAGGAGGTGCCGGCCGGACGCTACACGCTGGTTTTCCTCGCCGCCCCCGGACAGGCGGGCGTCGCCG
AGGTGGAACTCACCTACAATTGGGACCCGGAAGCCTATGGCGAGGGCCGCAACTTCGGCCATCTCGCCTTCGAGGTGGAC
GACATCTACGCCACCTGCGCCAGGCTGCAGGCAGCGGGCGTGACCATCAACCGCCCGCCCCGCGACGGCTACATGGCCTT
CGTGCGCTCGCCCGACAACATCTCCGTCGAACTGCTCCAGAAGGGCGGCGCCCGTGCCCCCGAGGAGCCCTGGGCCTCCC
TGCCGAACACTGGCAAGTGGTGA

Upstream 100 bases:

>100_bases
CCGCCAGTTCACGCTGGGCGGTCTTGAGATCGGCTTCGAAAATCATACCTTTGCCCCGCCGGCGCCCCGCCAGGTTTTCC
CTCCCCGCCAAGGATCAGCC

Downstream 100 bases:

>100_bases
GCATTCACCACTGAACGGGACGGGCTCCGGCAGGCCTCGGCCGCCGGAGCCCGTCTCTCCATCTGTCGCGCCTGTGAGGC
TGTTGGGTGGTGGACACCGG

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 140; Mature: 140

Protein sequence:

>140_residues
MRYLHTMVRVTDLDASLDFYCNKLGLVEVRRKEVPAGRYTLVFLAAPGQAGVAEVELTYNWDPEAYGEGRNFGHLAFEVD
DIYATCARLQAAGVTINRPPRDGYMAFVRSPDNISVELLQKGGARAPEEPWASLPNTGKW

Sequences:

>Translated_140_residues
MRYLHTMVRVTDLDASLDFYCNKLGLVEVRRKEVPAGRYTLVFLAAPGQAGVAEVELTYNWDPEAYGEGRNFGHLAFEVD
DIYATCARLQAAGVTINRPPRDGYMAFVRSPDNISVELLQKGGARAPEEPWASLPNTGKW
>Mature_140_residues
MRYLHTMVRVTDLDASLDFYCNKLGLVEVRRKEVPAGRYTLVFLAAPGQAGVAEVELTYNWDPEAYGEGRNFGHLAFEVD
DIYATCARLQAAGVTINRPPRDGYMAFVRSPDNISVELLQKGGARAPEEPWASLPNTGKW

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=142, Percent_Identity=32.3943661971831, Blast_Score=80, Evalue=8e-16,
Organism=Escherichia coli, GI1787940, Length=126, Percent_Identity=38.0952380952381, Blast_Score=92, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323639, Length=142, Percent_Identity=31.6901408450704, Blast_Score=65, Evalue=5e-12,
Organism=Drosophila melanogaster, GI20129743, Length=144, Percent_Identity=32.6388888888889, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 15593; Mature: 15593

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYLHTMVRVTDLDASLDFYCNKLGLVEVRRKEVPAGRYTLVFLAAPGQAGVAEVELTYN
CCHHHEEEEEECCCCCCHHHCCCCCEEEEHHHCCCCCCEEEEEEECCCCCCEEEEEEEEC
WDPEAYGEGRNFGHLAFEVDDIYATCARLQAAGVTINRPPRDGYMAFVRSPDNISVELLQ
CCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCEEEECCCCCCEEEEEECCCCCEEEEEE
KGGARAPEEPWASLPNTGKW
CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRYLHTMVRVTDLDASLDFYCNKLGLVEVRRKEVPAGRYTLVFLAAPGQAGVAEVELTYN
CCHHHEEEEEECCCCCCHHHCCCCCEEEEHHHCCCCCCEEEEEEECCCCCCEEEEEEEEC
WDPEAYGEGRNFGHLAFEVDDIYATCARLQAAGVTINRPPRDGYMAFVRSPDNISVELLQ
CCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCEEEECCCCCCEEEEEECCCCCEEEEEE
KGGARAPEEPWASLPNTGKW
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]