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The map label for this gene is fabI1 [H]

Identifier: 158423378

GI number: 158423378

Start: 2004064

End: 2004891

Strand: Reverse

Name: fabI1 [H]

Synonym: AZC_1754

Alternate gene names: 158423378

Gene position: 2004891-2004064 (Counterclockwise)

Preceding gene: 158423380

Following gene: 158423377

Centisome position: 37.34

GC content: 63.89

Gene sequence:

>828_bases
ATGGTGACCAATGACAGCGCGAGCGGTCTCCTCAAGGGCAAGCGCGGGCTGATCTTCGGCGTCGCCAACAACCGATCCAT
CGCTTACGGCATCGCCAAGGCGGCGCGCGCGCAGGGCGCGGAGATCGCCCTCACCTATCAGGGTGAAGCGCTGAAGAAGC
GCGTGGAACCGCTCGCCGCCGAACTCGGCGGCCATATCGTCGGCCATTGCGACGTCACCGATCCCGCCACCATCGACGCG
GTGTTCGCCGAGACCGAGAAGCTGTTCGGCTCCATCGACTTTCTCATCCACTGCATCGCCTTCTCCGACAAGGACGAGCT
GGATGGCCGCTATGTGGACACCACGGCGGACAATTTCACCAAGAGCATGTTCATCTCCTGCTACTCCTTTACGGCGCTGG
CCCAGCGCGCGGAGAAGCTGATGCCGAACGGCGGCTCGCTGGTGACCCTTACCTATTACGGCGCCGAGAAGTGGATGCCC
CATTACAACGTGATGGGTGTGGCCAAGGCGGCCCTTGAGGCCAGCGTGCGCTATCTTGCCGCCGACCTCGGCCCCAAGAA
CATCCGCGTCAATGCCATCTCGGCCGGCCCCATCAAGACGCTCGCCGCCTCCGGCATCGGCGATTTCCGCTACATCCTGA
AGTGGAACGAGCTGAATTCGCCGCTGCGCCGCACAGTCACCACCGACGAAGTGGGCGATGCGGGTGTCTACCTGATCTCC
GATCTCTCCCGCGGCGTTACGGGCGAGGTGCAGCACGTGGATGCGGGCTATCATATCGTCGGCATGAAGCACCCCGACGC
ACCGGACCTCGCCCTCGTGCGGGACTGA

Upstream 100 bases:

>100_bases
GGGCGTTCTCACACAGCCCTCGCTTGAACCCCCAACGCGCGCGTGCCATAGGAAGCGCCGCCCGGTGCGGGCCAACGATC
GAGCGACGGAGTACGTGGAC

Downstream 100 bases:

>100_bases
GCGCGGAAGGTGTGACCGGCAAGCGGCCTGCCCGGAGCGGCAGGCCGGAACGAACATCAGGAGACGATTGATGAAGCGGA
TGCTGCGCCCTGCCCTCTCG

Product: enoyl-acyl carrier protein reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 1 [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MVTNDSASGLLKGKRGLIFGVANNRSIAYGIAKAARAQGAEIALTYQGEALKKRVEPLAAELGGHIVGHCDVTDPATIDA
VFAETEKLFGSIDFLIHCIAFSDKDELDGRYVDTTADNFTKSMFISCYSFTALAQRAEKLMPNGGSLVTLTYYGAEKWMP
HYNVMGVAKAALEASVRYLAADLGPKNIRVNAISAGPIKTLAASGIGDFRYILKWNELNSPLRRTVTTDEVGDAGVYLIS
DLSRGVTGEVQHVDAGYHIVGMKHPDAPDLALVRD

Sequences:

>Translated_275_residues
MVTNDSASGLLKGKRGLIFGVANNRSIAYGIAKAARAQGAEIALTYQGEALKKRVEPLAAELGGHIVGHCDVTDPATIDA
VFAETEKLFGSIDFLIHCIAFSDKDELDGRYVDTTADNFTKSMFISCYSFTALAQRAEKLMPNGGSLVTLTYYGAEKWMP
HYNVMGVAKAALEASVRYLAADLGPKNIRVNAISAGPIKTLAASGIGDFRYILKWNELNSPLRRTVTTDEVGDAGVYLIS
DLSRGVTGEVQHVDAGYHIVGMKHPDAPDLALVRD
>Mature_275_residues
MVTNDSASGLLKGKRGLIFGVANNRSIAYGIAKAARAQGAEIALTYQGEALKKRVEPLAAELGGHIVGHCDVTDPATIDA
VFAETEKLFGSIDFLIHCIAFSDKDELDGRYVDTTADNFTKSMFISCYSFTALAQRAEKLMPNGGSLVTLTYYGAEKWMP
HYNVMGVAKAALEASVRYLAADLGPKNIRVNAISAGPIKTLAASGIGDFRYILKWNELNSPLRRTVTTDEVGDAGVYLIS
DLSRGVTGEVQHVDAGYHIVGMKHPDAPDLALVRD

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI10190704, Length=214, Percent_Identity=31.7757009345794, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI40254992, Length=207, Percent_Identity=28.9855072463768, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787545, Length=257, Percent_Identity=52.5291828793774, Blast_Score=268, Evalue=4e-73,
Organism=Escherichia coli, GI1789378, Length=260, Percent_Identity=28.8461538461538, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI2367365, Length=251, Percent_Identity=27.8884462151394, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787905, Length=240, Percent_Identity=26.6666666666667, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787335, Length=256, Percent_Identity=26.171875, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87082100, Length=263, Percent_Identity=27.7566539923954, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 29543; Mature: 29543

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTNDSASGLLKGKRGLIFGVANNRSIAYGIAKAARAQGAEIALTYQGEALKKRVEPLAA
CCCCCCCCCEEECCCEEEEEEECCCEEEHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHH
ELGGHIVGHCDVTDPATIDAVFAETEKLFGSIDFLIHCIAFSDKDELDGRYVDTTADNFT
HHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEECCHHHHH
KSMFISCYSFTALAQRAEKLMPNGGSLVTLTYYGAEKWMPHYNVMGVAKAALEASVRYLA
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHCCCCCCHHHHHHHHHHHHHHHHH
ADLGPKNIRVNAISAGPIKTLAASGIGDFRYILKWNELNSPLRRTVTTDEVGDAGVYLIS
HCCCCCCEEEEEEECCCHHHHHHCCCCCEEEEEEECCCCCHHHHCCCCCCCCCCCEEEEE
DLSRGVTGEVQHVDAGYHIVGMKHPDAPDLALVRD
HHCCCCCCCEEEECCCEEEEEECCCCCCCEEEECC
>Mature Secondary Structure
MVTNDSASGLLKGKRGLIFGVANNRSIAYGIAKAARAQGAEIALTYQGEALKKRVEPLAA
CCCCCCCCCEEECCCEEEEEEECCCEEEHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHH
ELGGHIVGHCDVTDPATIDAVFAETEKLFGSIDFLIHCIAFSDKDELDGRYVDTTADNFT
HHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEECCHHHHH
KSMFISCYSFTALAQRAEKLMPNGGSLVTLTYYGAEKWMPHYNVMGVAKAALEASVRYLA
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHCCCCCCHHHHHHHHHHHHHHHHH
ADLGPKNIRVNAISAGPIKTLAASGIGDFRYILKWNELNSPLRRTVTTDEVGDAGVYLIS
HCCCCCCEEEEEEECCCHHHHHHCCCCCEEEEEEECCCCCHHHHCCCCCCCCCCCEEEEE
DLSRGVTGEVQHVDAGYHIVGMKHPDAPDLALVRD
HHCCCCCCCEEEECCCEEEEEECCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]