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The map label for this gene is potD [H]

Identifier: 158423261

GI number: 158423261

Start: 1872182

End: 1873243

Strand: Direct

Name: potD [H]

Synonym: AZC_1637

Alternate gene names: 158423261

Gene position: 1872182-1873243 (Clockwise)

Preceding gene: 158423260

Following gene: 158423262

Centisome position: 34.87

GC content: 65.54

Gene sequence:

>1062_bases
ATGTCCGCCATCCTGACACGACGTCATTTCCTTGCGTCGGGTGCCGCTGCCGCGCTGGCGCCGGCGGCCTTGCCGGCATT
CTCCCGGGCCGCGCACGCGGCCGCGGGTCCGCTGGTCGCGACGACCTATCCGGGAACGTGGGAGCAGGCCCAGCGGGCCA
TTCTTCTCCCCGCCTTCACGAAGGCCACGGGTGCGAACGTGCTGCTGGAGCCTGTGCTGGCGCTCGAGGCGGTTTCAAAG
GTCTCGGCTTCGAAATCCAACCCGCCGTTCGACGTGGTGCTCATGGACGAGGGGCCCTATCTGGAAGCCCTCAACCGCGG
CATCTTCGCGCCGCTGCCGGTCGACAAGGTGACGAACCTGTCGGCCCTGCCGGAGCGCTTCATCGATCCGAACGGCATGG
GCGCCTTCATCACGGCTCAGGTCATGGGCATTGCGTACAATCCCAGCACCATCAAGACGCCGCCGACCTCGTGGCTCGAC
CTGTGGAAGCCCGAGTTCAAGGGGCGCGTGGGCATCACCGGTCTCGGCAGCAGTCTCGGCGCCGCCTGGATGGTGGAGAT
CGCCAAGCTCAACGGCGGCAGCGAGAGCAATATGGAGCCCGCGTTCGAGGCGGTGAAGCGGCTGTTGCCCAATCTGGGCG
CCGTGGCCGCCAATCCCGGTGCGCTCGCGACTCTGTTCCAGCAGGGCCAGATCGACATCAGCGTGAACTATCTCAACGCC
GTGCTGCCGCTGGCGCAGAATGGCGTGGAGATCGCCATCGCCCGCCCGGATTCGGGCTGGGTTCTGGTGCGCAACTCCCT
GCACGCCATCGCCAATGCGAAGAACCTCGATCTCGCCTACGCCTATATCAATGCCGCCCTGTCCGGCGAAGTGCAGGCAC
GGATGGCGGCGGCGCCGAACTTCCTGCTGCCCACGAACCGGACTGTCGCCTTCAGTTCCGAACTGCAGAAGGTGGCAAAG
TCCGAGGCGGAACTCTCCAAACTGACCCTCATCGACTGGAAGACGCTCAATCCCCAGCGCGCCGCACTCATCGAGCGCTT
CAACAGGGAAGTGCGAATCTAG

Upstream 100 bases:

>100_bases
AGGTTCGGGGCGGCGGACGGTCGTCGCCGGCGATCTCGCAGGTGGTCCTCATCGGCGGGCGCAGGTCTGCCTTGATCGAC
GGCCTTTTTGGAGAGACAGC

Downstream 100 bases:

>100_bases
GCCAAGACGCGCGGCTGCGGTCGAGCGATGATTGTCCATCCTCTGTTGGAAGAGGCGGGCCGACGGTCAAGGGGAGGGGG
GTCTTTGGGGGCTTCGGCGG

Product: ABC transporter substrate-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MSAILTRRHFLASGAAAALAPAALPAFSRAAHAAAGPLVATTYPGTWEQAQRAILLPAFTKATGANVLLEPVLALEAVSK
VSASKSNPPFDVVLMDEGPYLEALNRGIFAPLPVDKVTNLSALPERFIDPNGMGAFITAQVMGIAYNPSTIKTPPTSWLD
LWKPEFKGRVGITGLGSSLGAAWMVEIAKLNGGSESNMEPAFEAVKRLLPNLGAVAANPGALATLFQQGQIDISVNYLNA
VLPLAQNGVEIAIARPDSGWVLVRNSLHAIANAKNLDLAYAYINAALSGEVQARMAAAPNFLLPTNRTVAFSSELQKVAK
SEAELSKLTLIDWKTLNPQRAALIERFNREVRI

Sequences:

>Translated_353_residues
MSAILTRRHFLASGAAAALAPAALPAFSRAAHAAAGPLVATTYPGTWEQAQRAILLPAFTKATGANVLLEPVLALEAVSK
VSASKSNPPFDVVLMDEGPYLEALNRGIFAPLPVDKVTNLSALPERFIDPNGMGAFITAQVMGIAYNPSTIKTPPTSWLD
LWKPEFKGRVGITGLGSSLGAAWMVEIAKLNGGSESNMEPAFEAVKRLLPNLGAVAANPGALATLFQQGQIDISVNYLNA
VLPLAQNGVEIAIARPDSGWVLVRNSLHAIANAKNLDLAYAYINAALSGEVQARMAAAPNFLLPTNRTVAFSSELQKVAK
SEAELSKLTLIDWKTLNPQRAALIERFNREVRI
>Mature_352_residues
SAILTRRHFLASGAAAALAPAALPAFSRAAHAAAGPLVATTYPGTWEQAQRAILLPAFTKATGANVLLEPVLALEAVSKV
SASKSNPPFDVVLMDEGPYLEALNRGIFAPLPVDKVTNLSALPERFIDPNGMGAFITAQVMGIAYNPSTIKTPPTSWLDL
WKPEFKGRVGITGLGSSLGAAWMVEIAKLNGGSESNMEPAFEAVKRLLPNLGAVAANPGALATLFQQGQIDISVNYLNAV
LPLAQNGVEIAIARPDSGWVLVRNSLHAIANAKNLDLAYAYINAALSGEVQARMAAAPNFLLPTNRTVAFSSELQKVAKS
EAELSKLTLIDWKTLNPQRAALIERFNREVRI

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=292, Percent_Identity=26.3698630136986, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37454; Mature: 37322

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAILTRRHFLASGAAAALAPAALPAFSRAAHAAAGPLVATTYPGTWEQAQRAILLPAFT
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHCCCEEEEEEHH
KATGANVLLEPVLALEAVSKVSASKSNPPFDVVLMDEGPYLEALNRGIFAPLPVDKVTNL
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHCCCCCCCCCHHHHCCC
SALPERFIDPNGMGAFITAQVMGIAYNPSTIKTPPTSWLDLWKPEFKGRVGITGLGSSLG
HHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCCHHHHCCCHHHCCCEEEEECCCHHH
AAWMVEIAKLNGGSESNMEPAFEAVKRLLPNLGAVAANPGALATLFQQGQIDISVNYLNA
HHHHEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEEEEHHHHH
VLPLAQNGVEIAIARPDSGWVLVRNSLHAIANAKNLDLAYAYINAALSGEVQARMAAAPN
HHHHHCCCEEEEEECCCCCEEEEECCHHHHCCCCCCEEEEEEHHHHHCCCHHHHHHCCCC
FLLPTNRTVAFSSELQKVAKSEAELSKLTLIDWKTLNPQRAALIERFNREVRI
EECCCCCEEEHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SAILTRRHFLASGAAAALAPAALPAFSRAAHAAAGPLVATTYPGTWEQAQRAILLPAFT
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHCCCEEEEEEHH
KATGANVLLEPVLALEAVSKVSASKSNPPFDVVLMDEGPYLEALNRGIFAPLPVDKVTNL
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHCCCCCCCCCHHHHCCC
SALPERFIDPNGMGAFITAQVMGIAYNPSTIKTPPTSWLDLWKPEFKGRVGITGLGSSLG
HHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCCHHHHCCCHHHCCCEEEEECCCHHH
AAWMVEIAKLNGGSESNMEPAFEAVKRLLPNLGAVAANPGALATLFQQGQIDISVNYLNA
HHHHEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEEEEHHHHH
VLPLAQNGVEIAIARPDSGWVLVRNSLHAIANAKNLDLAYAYINAALSGEVQARMAAAPN
HHHHHCCCEEEEEECCCCCEEEEECCHHHHCCCCCCEEEEEEHHHHHCCCHHHHHHCCCC
FLLPTNRTVAFSSELQKVAKSEAELSKLTLIDWKTLNPQRAALIERFNREVRI
EECCCCCEEEHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]