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The map label for this gene is yjbN [H]

Identifier: 158423257

GI number: 158423257

Start: 1866157

End: 1867173

Strand: Reverse

Name: yjbN [H]

Synonym: AZC_1633

Alternate gene names: 158423257

Gene position: 1867173-1866157 (Counterclockwise)

Preceding gene: 158423258

Following gene: 158423256

Centisome position: 34.77

GC content: 68.53

Gene sequence:

>1017_bases
ATGCCCACCGAACAAAATTTTCGCTTTTCCATAGCCCCCATGATGGAGTGGACGGACCGCCACTGCCGGGCCTTCCATCG
CACCCTGTCGCGGCGGGCGTGGCTCTATACGGAGATGGTAACGGCCCCGGCCATCATCCATGGCGACCGGGCGCGGTTGC
TTGGCTATTCGCAGGCCGAGCACCCGGTGGTGGTGCAGCTTGGCGGCAGCAATCCCGCGCTTCTGGCGGAGGCCGCGCGC
ATCTGCGCGGATTTCGGCTATGACGAGATCAACCTCAATGTGGGCTGTCCCTCCGATCGAGTGAAGGGCGGCAATTTCGG
CGCCTGCCTCATGCGCGATCCGGCGCTGGTGGCCGAATGTGTCGCCGCCATGCGGGCCGCCGTTTCCATTCCCGTCACCG
TCAAATGCCGCATCGGTGTGGACGATCAGGACGTCGAGGAAGCCCTGGACGCGCTCGCGGATGCCGTGCTCGCGGCCGGC
TGCGCGCGCCTTATCGTGCATGCCCGCAAGGCCTGGCTGAAGGGCCTCTCGCCGAAGGAAAACCGCGAGATCCCGCCGCT
CGACTATGATCGCGTGCATCGTCTCAAGGCCCGCCTGCCCCACGTGCCCATCGAACTGAACGGCGGGCTCGTCACCCTTG
AAGACGCCCGCGCGGCCGGTGCCGGCCTCGACGGCGTGATGCTGGGCCGCGCCGCCTATCAGGATCCGGACCTGCTGCTG
GAAGTGGATGCGCAGGTGCATGGCGAGACCCCGCCCCATGCGGACGCCTTCGAGGCCATCGCCGCCTTCGAGCCCTATAT
CGCCGCGCATCTGGAGGCGGGCGGGCGGCTGCACGACATCACCCGCCACATGCTCGGCCTGTTCGGAGGTCGCCCGGGCG
CGCGCACCTATCGCCGCACGCTGGCGACGGAAGCGCTCGTGCCCGGCGCGGGCCTTGAGGTGCTGCGCAAGGCGGTTGCC
GCCATCCGCCGCCCCCTCGCGAAGGACGATGGCACACGGACGGGAACGGAGGGCTGA

Upstream 100 bases:

>100_bases
TCCATGTGCACACACACGTCGAAAATCGGAAATCGGGGCGTATCGTGGCGAAAAATCGAGTGCACACGACAAGACCCAAT
TCCTTGAAGTTATAAAAATA

Downstream 100 bases:

>100_bases
GCCCCTCCTGTCCTTCCGCAGAAGAAGCAGCCTTGGCCCCAACACGGCTTGCCAGACGCCTATGCGGATGATGTGGCACG
CGCGCAACAGGTGCACGGTG

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MPTEQNFRFSIAPMMEWTDRHCRAFHRTLSRRAWLYTEMVTAPAIIHGDRARLLGYSQAEHPVVVQLGGSNPALLAEAAR
ICADFGYDEINLNVGCPSDRVKGGNFGACLMRDPALVAECVAAMRAAVSIPVTVKCRIGVDDQDVEEALDALADAVLAAG
CARLIVHARKAWLKGLSPKENREIPPLDYDRVHRLKARLPHVPIELNGGLVTLEDARAAGAGLDGVMLGRAAYQDPDLLL
EVDAQVHGETPPHADAFEAIAAFEPYIAAHLEAGGRLHDITRHMLGLFGGRPGARTYRRTLATEALVPGAGLEVLRKAVA
AIRRPLAKDDGTRTGTEG

Sequences:

>Translated_338_residues
MPTEQNFRFSIAPMMEWTDRHCRAFHRTLSRRAWLYTEMVTAPAIIHGDRARLLGYSQAEHPVVVQLGGSNPALLAEAAR
ICADFGYDEINLNVGCPSDRVKGGNFGACLMRDPALVAECVAAMRAAVSIPVTVKCRIGVDDQDVEEALDALADAVLAAG
CARLIVHARKAWLKGLSPKENREIPPLDYDRVHRLKARLPHVPIELNGGLVTLEDARAAGAGLDGVMLGRAAYQDPDLLL
EVDAQVHGETPPHADAFEAIAAFEPYIAAHLEAGGRLHDITRHMLGLFGGRPGARTYRRTLATEALVPGAGLEVLRKAVA
AIRRPLAKDDGTRTGTEG
>Mature_337_residues
PTEQNFRFSIAPMMEWTDRHCRAFHRTLSRRAWLYTEMVTAPAIIHGDRARLLGYSQAEHPVVVQLGGSNPALLAEAARI
CADFGYDEINLNVGCPSDRVKGGNFGACLMRDPALVAECVAAMRAAVSIPVTVKCRIGVDDQDVEEALDALADAVLAAGC
ARLIVHARKAWLKGLSPKENREIPPLDYDRVHRLKARLPHVPIELNGGLVTLEDARAAGAGLDGVMLGRAAYQDPDLLLE
VDAQVHGETPPHADAFEAIAAFEPYIAAHLEAGGRLHDITRHMLGLFGGRPGARTYRRTLATEALVPGAGLEVLRKAVAA
IRRPLAKDDGTRTGTEG

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=307, Percent_Identity=26.7100977198697, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI239788483, Length=252, Percent_Identity=26.1904761904762, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI239788462, Length=252, Percent_Identity=26.1904761904762, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI40807366, Length=263, Percent_Identity=26.615969581749, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI145693211, Length=316, Percent_Identity=56.9620253164557, Blast_Score=355, Evalue=2e-99,
Organism=Escherichia coli, GI1788462, Length=224, Percent_Identity=32.1428571428571, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789660, Length=242, Percent_Identity=28.5123966942149, Blast_Score=81, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI25144369, Length=211, Percent_Identity=31.2796208530806, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17507177, Length=222, Percent_Identity=27.9279279279279, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=305, Percent_Identity=25.2459016393443, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24580595, Length=257, Percent_Identity=26.4591439688716, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19920448, Length=257, Percent_Identity=26.4591439688716, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24585320, Length=257, Percent_Identity=27.2373540856031, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36505; Mature: 36374

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTEQNFRFSIAPMMEWTDRHCRAFHRTLSRRAWLYTEMVTAPAIIHGDRARLLGYSQAE
CCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHCCCCCCC
HPVVVQLGGSNPALLAEAARICADFGYDEINLNVGCPSDRVKGGNFGACLMRDPALVAEC
CCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEECCHHHHHHH
VAAMRAAVSIPVTVKCRIGVDDQDVEEALDALADAVLAAGCARLIVHARKAWLKGLSPKE
HHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NREIPPLDYDRVHRLKARLPHVPIELNGGLVTLEDARAAGAGLDGVMLGRAAYQDPDLLL
CCCCCCCCHHHHHHHHHHCCCCCEEECCCEEEEECCCCCCCCCCHHHCCCHHCCCCCEEE
EVDAQVHGETPPHADAFEAIAAFEPYIAAHLEAGGRLHDITRHMLGLFGGRPGARTYRRT
EECCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHH
LATEALVPGAGLEVLRKAVAAIRRPLAKDDGTRTGTEG
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PTEQNFRFSIAPMMEWTDRHCRAFHRTLSRRAWLYTEMVTAPAIIHGDRARLLGYSQAE
CCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHCCCCCCC
HPVVVQLGGSNPALLAEAARICADFGYDEINLNVGCPSDRVKGGNFGACLMRDPALVAEC
CCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEECCHHHHHHH
VAAMRAAVSIPVTVKCRIGVDDQDVEEALDALADAVLAAGCARLIVHARKAWLKGLSPKE
HHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NREIPPLDYDRVHRLKARLPHVPIELNGGLVTLEDARAAGAGLDGVMLGRAAYQDPDLLL
CCCCCCCCHHHHHHHHHHCCCCCEEECCCEEEEECCCCCCCCCCHHHCCCHHCCCCCEEE
EVDAQVHGETPPHADAFEAIAAFEPYIAAHLEAGGRLHDITRHMLGLFGGRPGARTYRRT
EECCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHH
LATEALVPGAGLEVLRKAVAAIRRPLAKDDGTRTGTEG
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]