Click here to switch to the map view.

The map label for this gene is gatA1 [H]

Identifier: 158423139

GI number: 158423139

Start: 1724577

End: 1725992

Strand: Direct

Name: gatA1 [H]

Synonym: AZC_1515

Alternate gene names: 158423139

Gene position: 1724577-1725992 (Clockwise)

Preceding gene: 158423138

Following gene: 158423140

Centisome position: 32.12

GC content: 70.97

Gene sequence:

>1416_bases
ATGAACGCCCCGTCCTCCGTCGTCGCCGGCTACGCCACGGCGCCCGCCTCCGAGATTGCCGCCGCTGTGCGCTCTGGAGC
GGTGAGCGCGCGGGCGGTGGTCGATGCCGTGCTCGCCCGCATCGCCTCCGTTGATCCCAAGGTCAACGCATTCACCGACG
TCACCGCCGACCGCGCCCGCGCGGCCGCCGATGCACTGGACGCCCGCCGCGCGGCGGGCGAGACGCTGGGACCGCTCGCC
GGCGTGCCCTTCGCGGTGAAGAATCTGTTCGACATCAAGGGCTTGCCGACCCGCGCCGGCTCGCTGATCAACCGCGAGCT
TTCCCCCGCCGACCGCGACGCGACGCTGGTGGAGAAGCTGGAGGCGGCGGATGCCATCCTCTTGGGCGGGCTGAACATGG
GCGAATATGCCTATGATTTCACCGGCGAAAATGTCCACGACGGCCCCTCCCGCAATCCACATGACCTCGGCCACATGACC
GGCGGCTCGTCCGGCGGCTCGGGCGCGGCGGTGGCGGCGGGGCTGGTGCCGCTGACGCTCGGCTCGGACACCAATGGCTC
CATCCGCGTGCCGTCGTCCCTGTGCGGCATCTTCGGCCTGAAGCCCACCTATGGCCGCCTGTCGCGTGCCCATTCCTTCC
CCTTTGTCTCGGCCTTTGACCATCTCGGCCCCTTCGCCCGTTCGGTGGAGGATCTGGCGCTGGCTTATGACGCGATGCAG
GGGGCGGATGCCGCTGATCCCGTGCAGGTGGAGCGTCCGGTCGAGCCGACGACGCCCGCGTTGGGGCAGGGCATCGCGGA
CCTTAGGATTGCGTTGGCCGGCGGATATTTCCGCCAGAAGGGCATGCCGGAAGCCATTGCGGCGGTGGACCATGTGGCGG
CGGCGCTCGGCGTCTCCCGCACGGTCGAGCTGCCCGAGGCCGCCCGCGCCCGCGCGGCGGCCTATGTCATCACCGCCGCC
GAGGGGGCGAGCCTCCATGCGGAGAGGCTACGGACGAGGCCGCAGGATTTCGACCCGGCCGTGCGGGACCGTCTCATCGC
TGGCCTTGCCATTCCGGCCGCGTGGGTCGCCGGCGCGCAGCGCTTCCGCCGACATTTCCGGGCGCGGATGCTGAAGCTGT
TCGAGGAGGTGGACGTCATCCTTGCCCCCGCCACGCCCTGCCGCGCGCCGAAGCTGGGGCAGGCCACCATGGTGCTGGAC
GGCGTGGAACTGGCGGTGCGGCCGAACCTTGGCATTTACACGCAGCCCATCTCCTTCATCGGCCTGCCGGTGGCGGCGGT
ACCCGTGTGGCTCGATGGCGGCCTGCCCATCGGCGTGCAGGTCATCGCCCCGCCGTGGCGCGAGGATCTGGCGCTGCGCG
TGGCGTATCATCTGGAACAGGCCGGCGCCGCGCGCGCGCCGGTGGCGCAGGTTTGA

Upstream 100 bases:

>100_bases
AGGAGGCGCTGGCCAATCTGCGCTTCATTGCCGACGCCGCCCATCTCGTCCTGTCTTATGAGCTTGGCGACGAGGCCGAG
CCCGCCGCGGTCTATCACCC

Downstream 100 bases:

>100_bases
GCGGGAGAGGCGTTTTGGAGATTAACAATCCGGCCGTCGTGGCCGAGGTGGAAGCCGCGTTCGCCCGCTATGAACAGGCG
CTGGTCACCAATGATGTGGA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A 1 [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MNAPSSVVAGYATAPASEIAAAVRSGAVSARAVVDAVLARIASVDPKVNAFTDVTADRARAAADALDARRAAGETLGPLA
GVPFAVKNLFDIKGLPTRAGSLINRELSPADRDATLVEKLEAADAILLGGLNMGEYAYDFTGENVHDGPSRNPHDLGHMT
GGSSGGSGAAVAAGLVPLTLGSDTNGSIRVPSSLCGIFGLKPTYGRLSRAHSFPFVSAFDHLGPFARSVEDLALAYDAMQ
GADAADPVQVERPVEPTTPALGQGIADLRIALAGGYFRQKGMPEAIAAVDHVAAALGVSRTVELPEAARARAAAYVITAA
EGASLHAERLRTRPQDFDPAVRDRLIAGLAIPAAWVAGAQRFRRHFRARMLKLFEEVDVILAPATPCRAPKLGQATMVLD
GVELAVRPNLGIYTQPISFIGLPVAAVPVWLDGGLPIGVQVIAPPWREDLALRVAYHLEQAGAARAPVAQV

Sequences:

>Translated_471_residues
MNAPSSVVAGYATAPASEIAAAVRSGAVSARAVVDAVLARIASVDPKVNAFTDVTADRARAAADALDARRAAGETLGPLA
GVPFAVKNLFDIKGLPTRAGSLINRELSPADRDATLVEKLEAADAILLGGLNMGEYAYDFTGENVHDGPSRNPHDLGHMT
GGSSGGSGAAVAAGLVPLTLGSDTNGSIRVPSSLCGIFGLKPTYGRLSRAHSFPFVSAFDHLGPFARSVEDLALAYDAMQ
GADAADPVQVERPVEPTTPALGQGIADLRIALAGGYFRQKGMPEAIAAVDHVAAALGVSRTVELPEAARARAAAYVITAA
EGASLHAERLRTRPQDFDPAVRDRLIAGLAIPAAWVAGAQRFRRHFRARMLKLFEEVDVILAPATPCRAPKLGQATMVLD
GVELAVRPNLGIYTQPISFIGLPVAAVPVWLDGGLPIGVQVIAPPWREDLALRVAYHLEQAGAARAPVAQV
>Mature_471_residues
MNAPSSVVAGYATAPASEIAAAVRSGAVSARAVVDAVLARIASVDPKVNAFTDVTADRARAAADALDARRAAGETLGPLA
GVPFAVKNLFDIKGLPTRAGSLINRELSPADRDATLVEKLEAADAILLGGLNMGEYAYDFTGENVHDGPSRNPHDLGHMT
GGSSGGSGAAVAAGLVPLTLGSDTNGSIRVPSSLCGIFGLKPTYGRLSRAHSFPFVSAFDHLGPFARSVEDLALAYDAMQ
GADAADPVQVERPVEPTTPALGQGIADLRIALAGGYFRQKGMPEAIAAVDHVAAALGVSRTVELPEAARARAAAYVITAA
EGASLHAERLRTRPQDFDPAVRDRLIAGLAIPAAWVAGAQRFRRHFRARMLKLFEEVDVILAPATPCRAPKLGQATMVLD
GVELAVRPNLGIYTQPISFIGLPVAAVPVWLDGGLPIGVQVIAPPWREDLALRVAYHLEQAGAARAPVAQV

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=494, Percent_Identity=29.5546558704453, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI222831590, Length=511, Percent_Identity=29.3542074363992, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI166795287, Length=522, Percent_Identity=25.6704980842912, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=430, Percent_Identity=27.6744186046512, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17537465, Length=251, Percent_Identity=32.2709163346614, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17556264, Length=219, Percent_Identity=31.5068493150685, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=220, Percent_Identity=27.2727272727273, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=190, Percent_Identity=31.5789473684211, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=190, Percent_Identity=31.5789473684211, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71990152, Length=525, Percent_Identity=21.1428571428571, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17538254, Length=192, Percent_Identity=27.0833333333333, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=399, Percent_Identity=30.3258145363409, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323950, Length=464, Percent_Identity=25.6465517241379, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320448, Length=222, Percent_Identity=31.981981981982, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24648113, Length=485, Percent_Identity=29.8969072164948, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24644968, Length=491, Percent_Identity=27.9022403258656, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24652985, Length=486, Percent_Identity=28.3950617283951, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19922090, Length=486, Percent_Identity=28.3950617283951, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24652981, Length=486, Percent_Identity=28.3950617283951, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24652983, Length=486, Percent_Identity=28.3950617283951, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI45550774, Length=505, Percent_Identity=25.9405940594059, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24648435, Length=505, Percent_Identity=25.9405940594059, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24648437, Length=505, Percent_Identity=25.9405940594059, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI161078093, Length=504, Percent_Identity=27.5793650793651, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21356731, Length=230, Percent_Identity=34.3478260869565, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24648441, Length=438, Percent_Identity=25.5707762557078, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24648439, Length=438, Percent_Identity=25.5707762557078, Blast_Score=99, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 48912; Mature: 48912

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPSSVVAGYATAPASEIAAAVRSGAVSARAVVDAVLARIASVDPKVNAFTDVTADRAR
CCCCCHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHCHHHHHH
AAADALDARRAAGETLGPLAGVPFAVKNLFDIKGLPTRAGSLINRELSPADRDATLVEKL
HHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHH
EAADAILLGGLNMGEYAYDFTGENVHDGPSRNPHDLGHMTGGSSGGSGAAVAAGLVPLTL
HHHCEEEECCCCCCCEEEECCCCCCCCCCCCCCCHHCCCCCCCCCCCCHHHHHCCEEEEE
GSDTNGSIRVPSSLCGIFGLKPTYGRLSRAHSFPFVSAFDHLGPFARSVEDLALAYDAMQ
CCCCCCCEECCHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHC
GADAADPVQVERPVEPTTPALGQGIADLRIALAGGYFRQKGMPEAIAAVDHVAAALGVSR
CCCCCCCCEECCCCCCCCCHHHCCHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHCCHH
TVELPEAARARAAAYVITAAEGASLHAERLRTRPQDFDPAVRDRLIAGLAIPAAWVAGAQ
CCCCCHHHHHHHEEEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RFRRHFRARMLKLFEEVDVILAPATPCRAPKLGQATMVLDGVELAVRPNLGIYTQPISFI
HHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCEEEEEECEEEEECCCCCEEECCHHHH
GLPVAAVPVWLDGGLPIGVQVIAPPWREDLALRVAYHLEQAGAARAPVAQV
CCCHHHEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNAPSSVVAGYATAPASEIAAAVRSGAVSARAVVDAVLARIASVDPKVNAFTDVTADRAR
CCCCCHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHCHHHHHH
AAADALDARRAAGETLGPLAGVPFAVKNLFDIKGLPTRAGSLINRELSPADRDATLVEKL
HHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHH
EAADAILLGGLNMGEYAYDFTGENVHDGPSRNPHDLGHMTGGSSGGSGAAVAAGLVPLTL
HHHCEEEECCCCCCCEEEECCCCCCCCCCCCCCCHHCCCCCCCCCCCCHHHHHCCEEEEE
GSDTNGSIRVPSSLCGIFGLKPTYGRLSRAHSFPFVSAFDHLGPFARSVEDLALAYDAMQ
CCCCCCCEECCHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHC
GADAADPVQVERPVEPTTPALGQGIADLRIALAGGYFRQKGMPEAIAAVDHVAAALGVSR
CCCCCCCCEECCCCCCCCCHHHCCHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHCCHH
TVELPEAARARAAAYVITAAEGASLHAERLRTRPQDFDPAVRDRLIAGLAIPAAWVAGAQ
CCCCCHHHHHHHEEEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RFRRHFRARMLKLFEEVDVILAPATPCRAPKLGQATMVLDGVELAVRPNLGIYTQPISFI
HHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCEEEEEECEEEEECCCCCEEECCHHHH
GLPVAAVPVWLDGGLPIGVQVIAPPWREDLALRVAYHLEQAGAARAPVAQV
CCCHHHEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]