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The map label for this gene is potF [H]

Identifier: 158423089

GI number: 158423089

Start: 1664292

End: 1665398

Strand: Direct

Name: potF [H]

Synonym: AZC_1465

Alternate gene names: 158423089

Gene position: 1664292-1665398 (Clockwise)

Preceding gene: 158423088

Following gene: 158423094

Centisome position: 30.99

GC content: 59.53

Gene sequence:

>1107_bases
ATGCGCCGGTTCGTTCTTGCGGCGGTGCTGGGGGCTGCGGCGATCGTCGCCGGTCCCGCACTCGCGCAGCAGAAGGTGGT
GAACGTCTATAATTGGTCGGACTATATCGACGAGAGCGTGCTGGAGGAGTTCACGAAGGAAACGGGCATCAAGGTCCGCT
ACGACGTGTTCGACAGCAACGAGGTGCTGGAGACCAAGCTGCTCGCCGGCAAGACCGGCTATGACGTGGTGGTGCCGTCC
GGCTCCTTCATGCAGCGCCAGATCAAGGCCGGCGTCTTCATCAAGCTCGACAAGTCGAAGCTGCCGAACCTGCAATATGC
CTGGCCCGAGATCACCAAGCGGCTCGCCGTCTATGATCCCGGCAATGAATATGCCGTCAATTACATGTGGGGCACCACCG
GCATCGGCTACAATGTGGACATGGTGAAGCAGCGCCTTGGCGATGTGCCCATGAACACCTGGGACATCGTCTTCAAGCCG
GAGAACCTCTCCAAGCTGAAGGACTGCGGCGTCTACATGCTGGACGGGCCGGAGGAGATCATTCCTACCGCCATGCGGTA
TCTGGGCCTCGATCCGGACAGCAAGAAGCCGGAGGACATCACCAAGGCCGGCGAACTGCTGATGAAGATCCGGCCGTTCA
TCCGCAAGTTCGATTCCTCCGGCTATATCAACGCGCTGGCGCGCGGCGACATCTGTCTGGCGGTGGGCTGGTCGGGTGAC
GTCTTCCAGGCCAAGGCCCGCGCCGAGGAAGCCGCCAAGAAGACCAAGAAGAAGGTTGTGCCCGTGGCCTATGCGCTGCC
GAAGGAAGGCGCGCTGATGTGGTTCGACAATTTCGCCATTCCGAAGGACGCCAAGAATATCGATGAGGCGCTGACCTTCA
TCAATTTCATGATGCGTCCGGAGATCGCCGCCAAGAACTCGAACTTCATCTCATACGCCAACGGCAATCTCGCCTCGCAG
AAGTTCATCGACAAGGCGGTCCTGGACAATCCGGCCATCTATCCGACGCCGGAAGTCTTCGCTTTGCTGTTCACCGTGAC
GACGAATCCGCAGGATGTGCAGCGGGTCATCACCAAGACCTGGAACACCTTCAAGCGCGGCAAATGA

Upstream 100 bases:

>100_bases
CCTTCTGCCCATGCGCAGGAGTTGAGCCTTTCGCGCTGCCGCAATAGCCTTCTGAATCGGGGGGGGCGGGTCCGCCCTGC
CTGACCAGAGGGAGAACAAG

Downstream 100 bases:

>100_bases
AACGTTCACTCAGGTGAAACGAGAAGGGCCGCCGGCATCGCCTGCGGCCCTTCTTCGAGGTGTGCCCTCAACTCAGGCGA
CGCGGCCCCCCATCTTGAAG

Product: ABC transporter substrate-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MRRFVLAAVLGAAAIVAGPALAQQKVVNVYNWSDYIDESVLEEFTKETGIKVRYDVFDSNEVLETKLLAGKTGYDVVVPS
GSFMQRQIKAGVFIKLDKSKLPNLQYAWPEITKRLAVYDPGNEYAVNYMWGTTGIGYNVDMVKQRLGDVPMNTWDIVFKP
ENLSKLKDCGVYMLDGPEEIIPTAMRYLGLDPDSKKPEDITKAGELLMKIRPFIRKFDSSGYINALARGDICLAVGWSGD
VFQAKARAEEAAKKTKKKVVPVAYALPKEGALMWFDNFAIPKDAKNIDEALTFINFMMRPEIAAKNSNFISYANGNLASQ
KFIDKAVLDNPAIYPTPEVFALLFTVTTNPQDVQRVITKTWNTFKRGK

Sequences:

>Translated_368_residues
MRRFVLAAVLGAAAIVAGPALAQQKVVNVYNWSDYIDESVLEEFTKETGIKVRYDVFDSNEVLETKLLAGKTGYDVVVPS
GSFMQRQIKAGVFIKLDKSKLPNLQYAWPEITKRLAVYDPGNEYAVNYMWGTTGIGYNVDMVKQRLGDVPMNTWDIVFKP
ENLSKLKDCGVYMLDGPEEIIPTAMRYLGLDPDSKKPEDITKAGELLMKIRPFIRKFDSSGYINALARGDICLAVGWSGD
VFQAKARAEEAAKKTKKKVVPVAYALPKEGALMWFDNFAIPKDAKNIDEALTFINFMMRPEIAAKNSNFISYANGNLASQ
KFIDKAVLDNPAIYPTPEVFALLFTVTTNPQDVQRVITKTWNTFKRGK
>Mature_368_residues
MRRFVLAAVLGAAAIVAGPALAQQKVVNVYNWSDYIDESVLEEFTKETGIKVRYDVFDSNEVLETKLLAGKTGYDVVVPS
GSFMQRQIKAGVFIKLDKSKLPNLQYAWPEITKRLAVYDPGNEYAVNYMWGTTGIGYNVDMVKQRLGDVPMNTWDIVFKP
ENLSKLKDCGVYMLDGPEEIIPTAMRYLGLDPDSKKPEDITKAGELLMKIRPFIRKFDSSGYINALARGDICLAVGWSGD
VFQAKARAEEAAKKTKKKVVPVAYALPKEGALMWFDNFAIPKDAKNIDEALTFINFMMRPEIAAKNSNFISYANGNLASQ
KFIDKAVLDNPAIYPTPEVFALLFTVTTNPQDVQRVITKTWNTFKRGK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=370, Percent_Identity=52.4324324324324, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1787367, Length=312, Percent_Identity=34.9358974358974, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI1787711, Length=294, Percent_Identity=24.1496598639456, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 41189; Mature: 41189

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRFVLAAVLGAAAIVAGPALAQQKVVNVYNWSDYIDESVLEEFTKETGIKVRYDVFDSN
CCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCC
EVLETKLLAGKTGYDVVVPSGSFMQRQIKAGVFIKLDKSKLPNLQYAWPEITKRLAVYDP
HHHHHHHHCCCCCCEEEECCCHHHHHHHCCCEEEEECCCCCCCCEECCHHHHCEEEEECC
GNEYAVNYMWGTTGIGYNVDMVKQRLGDVPMNTWDIVFKPENLSKLKDCGVYMLDGPEEI
CCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHCCEEEECCCHHH
IPTAMRYLGLDPDSKKPEDITKAGELLMKIRPFIRKFDSSGYINALARGDICLAVGWSGD
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCC
VFQAKARAEEAAKKTKKKVVPVAYALPKEGALMWFDNFAIPKDAKNIDEALTFINFMMRP
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCC
EIAAKNSNFISYANGNLASQKFIDKAVLDNPAIYPTPEVFALLFTVTTNPQDVQRVITKT
CHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHEEEEEEECCCHHHHHHHHHHH
WNTFKRGK
HHHHCCCC
>Mature Secondary Structure
MRRFVLAAVLGAAAIVAGPALAQQKVVNVYNWSDYIDESVLEEFTKETGIKVRYDVFDSN
CCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCC
EVLETKLLAGKTGYDVVVPSGSFMQRQIKAGVFIKLDKSKLPNLQYAWPEITKRLAVYDP
HHHHHHHHCCCCCCEEEECCCHHHHHHHCCCEEEEECCCCCCCCEECCHHHHCEEEEECC
GNEYAVNYMWGTTGIGYNVDMVKQRLGDVPMNTWDIVFKPENLSKLKDCGVYMLDGPEEI
CCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHCCEEEECCCHHH
IPTAMRYLGLDPDSKKPEDITKAGELLMKIRPFIRKFDSSGYINALARGDICLAVGWSGD
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCC
VFQAKARAEEAAKKTKKKVVPVAYALPKEGALMWFDNFAIPKDAKNIDEALTFINFMMRP
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCC
EIAAKNSNFISYANGNLASQKFIDKAVLDNPAIYPTPEVFALLFTVTTNPQDVQRVITKT
CHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHEEEEEEECCCHHHHHHHHHHH
WNTFKRGK
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]