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The map label for this gene is rbsB [H]

Identifier: 158423043

GI number: 158423043

Start: 1614929

End: 1615903

Strand: Direct

Name: rbsB [H]

Synonym: AZC_1419

Alternate gene names: 158423043

Gene position: 1614929-1615903 (Clockwise)

Preceding gene: 158423042

Following gene: 158423044

Centisome position: 30.07

GC content: 63.38

Gene sequence:

>975_bases
ATGTCGAAGCTCAAGCTGTTCGGAGTGGTGCTGCCCGCCGCCTTGCTGGCCACCAGCGCCTTTGCGGCGGATGAGACCAT
CGCCGTCTTCACCAAGAACCAGACCAATCCCTATTTCCAGACCGTGCGCGTGGGCGCCGAAGCCGCAGCCAAGGCCATGG
GCGCCAAGGTGGTGCACTACATTCCCACCAAGCCCGACTCCATCCCCGAGCAGCTCAGCCAGATCGAGGATGTGATCGTG
AAGAAGCCGAGCGCCATCGTCTTCGTGCCGGTGGACTACAAGGCCATGGTGCCCGGCGTCGAGCAGATCAACGATGCCAA
GATTCCCGTCGTCAATGTCACGGACCGCAGCGCCGGCGGCAAGTTCCTCGCCTTCGTCGGCGCGGACGACTACAGCCTCG
GCCTCGAGACCGGCCGTTATCTGCTCAAGGAGATGGGCGGCAAGGGCAACGTGGTGATCCTGGAAGGCGTGAAGGGTTCC
CTCACCAATGTGGACCGGGTGCGCGGCTTCAATGATGCGCTCAAGGAATTCAAGGACGTGAAGCTGCTCGCCAGCCAGCC
GGCCAATTACCAGCGCCTGCAGGGGTTGCAGGTGATGGAAAACCTCATGCAGTCGCACCCGCAGATCGACGGCGTGCTGG
CGGCCAACGACGCCATGGCCATCGGCGCCATCGAGGCACTGGACGGGGCCAACCGCAAGGCCAAGGTGGTCGGCATCAAC
GGCACCAAGGAGGCGGTGGATGCCATCAAGTCCGGCAAGCTGCTGGCGTCCGGTGATTACAACGGCTTCCTCCAGGGCTG
CATCGGCACCATGGTCGCCATCCGCGCCCTGCGCAAGGAGACCATCGTCAATGAGGTGGTGCTGAAGCCCACCGTCGTGA
ATGCCTCGAACTACAAGCCCTATGACGTGCCGCTGGAGAGCCGTTCCTGCCCGAGCTGGTCGGATGCTGCGGCCCTGTCG
GTGAAGACCAACTGA

Upstream 100 bases:

>100_bases
ACCGGCGCGATCCTCGTCTTCGCCGTCTGGCTCGACATGTACGGCCGCAAGAAGGCCTGAGCCGCGTCACCAATCCAGAA
TAATTACCGGAGGAAACCCA

Downstream 100 bases:

>100_bases
GACGGCAGAGCCGTCACGCTTTGAAGAGAGAAGAGGGCGCTTTCCCGATGGCCTGGACCGGGACCCGCTCCGGCCATCGG
GAGGCGCTTTCGCCCCATGG

Product: sugar ABC transporter substrate-binding protein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSKLKLFGVVLPAALLATSAFAADETIAVFTKNQTNPYFQTVRVGAEAAAKAMGAKVVHYIPTKPDSIPEQLSQIEDVIV
KKPSAIVFVPVDYKAMVPGVEQINDAKIPVVNVTDRSAGGKFLAFVGADDYSLGLETGRYLLKEMGGKGNVVILEGVKGS
LTNVDRVRGFNDALKEFKDVKLLASQPANYQRLQGLQVMENLMQSHPQIDGVLAANDAMAIGAIEALDGANRKAKVVGIN
GTKEAVDAIKSGKLLASGDYNGFLQGCIGTMVAIRALRKETIVNEVVLKPTVVNASNYKPYDVPLESRSCPSWSDAAALS
VKTN

Sequences:

>Translated_324_residues
MSKLKLFGVVLPAALLATSAFAADETIAVFTKNQTNPYFQTVRVGAEAAAKAMGAKVVHYIPTKPDSIPEQLSQIEDVIV
KKPSAIVFVPVDYKAMVPGVEQINDAKIPVVNVTDRSAGGKFLAFVGADDYSLGLETGRYLLKEMGGKGNVVILEGVKGS
LTNVDRVRGFNDALKEFKDVKLLASQPANYQRLQGLQVMENLMQSHPQIDGVLAANDAMAIGAIEALDGANRKAKVVGIN
GTKEAVDAIKSGKLLASGDYNGFLQGCIGTMVAIRALRKETIVNEVVLKPTVVNASNYKPYDVPLESRSCPSWSDAAALS
VKTN
>Mature_323_residues
SKLKLFGVVLPAALLATSAFAADETIAVFTKNQTNPYFQTVRVGAEAAAKAMGAKVVHYIPTKPDSIPEQLSQIEDVIVK
KPSAIVFVPVDYKAMVPGVEQINDAKIPVVNVTDRSAGGKFLAFVGADDYSLGLETGRYLLKEMGGKGNVVILEGVKGSL
TNVDRVRGFNDALKEFKDVKLLASQPANYQRLQGLQVMENLMQSHPQIDGVLAANDAMAIGAIEALDGANRKAKVVGING
TKEAVDAIKSGKLLASGDYNGFLQGCIGTMVAIRALRKETIVNEVVLKPTVVNASNYKPYDVPLESRSCPSWSDAAALSV
KTN

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=260, Percent_Identity=33.4615384615385, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1790526, Length=266, Percent_Identity=33.0827067669173, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1790674, Length=173, Percent_Identity=32.9479768786127, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 34510; Mature: 34379

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLKLFGVVLPAALLATSAFAADETIAVFTKNQTNPYFQTVRVGAEAAAKAMGAKVVHY
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEE
IPTKPDSIPEQLSQIEDVIVKKPSAIVFVPVDYKAMVPGVEQINDAKIPVVNVTDRSAGG
ECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHCCCHHHCCCCCCEEEEECCCCCCC
KFLAFVGADDYSLGLETGRYLLKEMGGKGNVVILEGVKGSLTNVDRVRGFNDALKEFKDV
CEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHCCHHHHHHHHHHH
KLLASQPANYQRLQGLQVMENLMQSHPQIDGVLAANDAMAIGAIEALDGANRKAKVVGIN
HHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEHHHHHHCCCCCEEEEEEEC
GTKEAVDAIKSGKLLASGDYNGFLQGCIGTMVAIRALRKETIVNEVVLKPTVVNASNYKP
CCHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCC
YDVPLESRSCPSWSDAAALSVKTN
CCCCCCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure 
SKLKLFGVVLPAALLATSAFAADETIAVFTKNQTNPYFQTVRVGAEAAAKAMGAKVVHY
CCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEE
IPTKPDSIPEQLSQIEDVIVKKPSAIVFVPVDYKAMVPGVEQINDAKIPVVNVTDRSAGG
ECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHCCCHHHCCCCCCEEEEECCCCCCC
KFLAFVGADDYSLGLETGRYLLKEMGGKGNVVILEGVKGSLTNVDRVRGFNDALKEFKDV
CEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHCCHHHHHHHHHHH
KLLASQPANYQRLQGLQVMENLMQSHPQIDGVLAANDAMAIGAIEALDGANRKAKVVGIN
HHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEHHHHHHCCCCCEEEEEEEC
GTKEAVDAIKSGKLLASGDYNGFLQGCIGTMVAIRALRKETIVNEVVLKPTVVNASNYKP
CCHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCC
YDVPLESRSCPSWSDAAALSVKTN
CCCCCCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]