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The map label for this gene is ilvD [H]

Identifier: 158423032

GI number: 158423032

Start: 1602419

End: 1604092

Strand: Direct

Name: ilvD [H]

Synonym: AZC_1408

Alternate gene names: 158423032

Gene position: 1602419-1604092 (Clockwise)

Preceding gene: 158423030

Following gene: 158423034

Centisome position: 29.84

GC content: 68.22

Gene sequence:

>1674_bases
ATGCGCCGCAACCTTGCCAGCTATGGCGATCCGGGCTTTTCGCTCTTCCTGCGCAAGGCCTTCATCAAGGCGGCCGGCTA
TTCGGACGATGCGCTGGATCGGCCGATTATCGGCATCACCAACACCTTCAGCGACTTCAATCCCTGCCACGGCAACGTGC
CGCGCCTCATCGAGGCGGTGAAGCGCGGGGTGATGCTCGCCGGCGGTCTGCCGATGGAGTTTCCCACCGTCTCCATCCAC
GAATCCTTCTCCAACCCCACCTCCATGTTCCTGCGCAATCTCATGGCCATGGATACGGAGGAGATGATCCGCGCCCAGCC
CATGGACGCGGTGGTGCTGATCGGCGGCTGCGACAAGACCGTGCCGGCCCAGCTCATGGGCGCGGTGAGCGCCAATGTGC
CCGCAATCCAGCTCATCACCGGGCCGATGCTGGTCGGCCATCACAAGGGCGAAATCCTCGGCGCCTGCACCGATTGCCGG
CGGCTCTGGGGCCAGCACCGGGCCGGGCATCTCGATGCGGAGGAGATGGACGTGCTCTCGGGCCGCCTCGCGCCGACGCA
GGGCACCTGCATGGTCATGGGCACGGCCAGCACCATGGGCTGTCTCGTGGAGGCGCTGGGCATCGCCTTGCCGTGGACGG
GCACCATTCCCGCCACCCACGCGGACCGCATCCGTGCGGCCGAGGCGAGCGGGCGTCAGGCGGTGCAACTGGCAACCCAT
GGGCCGACGCCGCGCGATCTGCTCACGCCTGCCGCCTTCCGCAATGCGCTGACGGTGCTTCAGGCCATTGGCGGCTCCAC
CAACGGGCTCGTGCATCTCGCCGCCATCGCCCGCCGGGCCGGCCTCTCCGTCGATCTCGACGCCTTCGATGCCATCGGTC
GATCCGTGCCTGTGCTCATCGACCTGAAGCCCTCGGGCGACCATTACATGGAGCACTTTCACTGGGCCGGCGGCGTGCCG
AAGCTGATGAAAGAGCTGGGCGATCACCTCGACCGCAGCGCGCCCACCATCGACGGACGTACCATCGGCGAGATTGCGGA
CGCCGCCGAGGAGGTGCCCGGCCAAGCCATCATCCGCCCCGCCAGCGCGCCGCTGAAGCCGTTCGGCGGCATGGCCGTGC
TGCGCGGCAATCTGGCGCCGGGCGGCGCGGTCATCAAGCATTCCGCCGCCTCCGAGGCCCTGCTGCGCCACACCGGGCGG
GCGGTGGTCTTCGACAGCGTCGAGGACATGGTGAACCGCATCGACGATCCGGGTCTTGATGTGCAGGCGGATGACGTATT
GGTGCTGCGCAACGCCGGCCCCAAGGGCGCGCCGGGCATGCCGGAGGCCGGCTATCTGCCGATCCCGAAGAAGCTGGCGC
AGGCGGGCGTGAAGGACATGGTGCGGATTTCCGATGCCCGGATGAGCGGCACGGCCTTTGGCACCATCGTTCTGCACATC
ACGCCGGAGGCGGCGGTCGGCGGCCCGCTCGGTCTCGTGCGCAGCGGCGACACCATCCGCCTCGACGTGGCGGCACGCGA
GATCGCGCTGCTGGTGCCGGATGAGGAGCTCGAACGCCGCAGGGCGGAATGGACGCCCCCCGCCCATCTGGCCGAGGCGG
CGCGCGGCTATCAGCACCTTTATCTTACCCACGTCCTTCAGGCGGACGAGGGGTGCGATTTCGATTTCTGCTGA

Upstream 100 bases:

>100_bases
CGAAATAACGCTGTTCGATATTGTTGACAATCCTGGCAACAACCGCTTCCAATGCCCCATGACCAGCGCCGATGACGAGA
CCCCACCCGCCGCCCGCGGC

Downstream 100 bases:

>100_bases
CGGGTCGCGTCGGTGCGCTCACGCGGACCAGATGCCGCCGTCCGAGGTCCGCCGCACGTAATTGACCAGGAAGTCCGTGA
AGACGCGGATCTTGGCCGGC

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MRRNLASYGDPGFSLFLRKAFIKAAGYSDDALDRPIIGITNTFSDFNPCHGNVPRLIEAVKRGVMLAGGLPMEFPTVSIH
ESFSNPTSMFLRNLMAMDTEEMIRAQPMDAVVLIGGCDKTVPAQLMGAVSANVPAIQLITGPMLVGHHKGEILGACTDCR
RLWGQHRAGHLDAEEMDVLSGRLAPTQGTCMVMGTASTMGCLVEALGIALPWTGTIPATHADRIRAAEASGRQAVQLATH
GPTPRDLLTPAAFRNALTVLQAIGGSTNGLVHLAAIARRAGLSVDLDAFDAIGRSVPVLIDLKPSGDHYMEHFHWAGGVP
KLMKELGDHLDRSAPTIDGRTIGEIADAAEEVPGQAIIRPASAPLKPFGGMAVLRGNLAPGGAVIKHSAASEALLRHTGR
AVVFDSVEDMVNRIDDPGLDVQADDVLVLRNAGPKGAPGMPEAGYLPIPKKLAQAGVKDMVRISDARMSGTAFGTIVLHI
TPEAAVGGPLGLVRSGDTIRLDVAAREIALLVPDEELERRRAEWTPPAHLAEAARGYQHLYLTHVLQADEGCDFDFC

Sequences:

>Translated_557_residues
MRRNLASYGDPGFSLFLRKAFIKAAGYSDDALDRPIIGITNTFSDFNPCHGNVPRLIEAVKRGVMLAGGLPMEFPTVSIH
ESFSNPTSMFLRNLMAMDTEEMIRAQPMDAVVLIGGCDKTVPAQLMGAVSANVPAIQLITGPMLVGHHKGEILGACTDCR
RLWGQHRAGHLDAEEMDVLSGRLAPTQGTCMVMGTASTMGCLVEALGIALPWTGTIPATHADRIRAAEASGRQAVQLATH
GPTPRDLLTPAAFRNALTVLQAIGGSTNGLVHLAAIARRAGLSVDLDAFDAIGRSVPVLIDLKPSGDHYMEHFHWAGGVP
KLMKELGDHLDRSAPTIDGRTIGEIADAAEEVPGQAIIRPASAPLKPFGGMAVLRGNLAPGGAVIKHSAASEALLRHTGR
AVVFDSVEDMVNRIDDPGLDVQADDVLVLRNAGPKGAPGMPEAGYLPIPKKLAQAGVKDMVRISDARMSGTAFGTIVLHI
TPEAAVGGPLGLVRSGDTIRLDVAAREIALLVPDEELERRRAEWTPPAHLAEAARGYQHLYLTHVLQADEGCDFDFC
>Mature_557_residues
MRRNLASYGDPGFSLFLRKAFIKAAGYSDDALDRPIIGITNTFSDFNPCHGNVPRLIEAVKRGVMLAGGLPMEFPTVSIH
ESFSNPTSMFLRNLMAMDTEEMIRAQPMDAVVLIGGCDKTVPAQLMGAVSANVPAIQLITGPMLVGHHKGEILGACTDCR
RLWGQHRAGHLDAEEMDVLSGRLAPTQGTCMVMGTASTMGCLVEALGIALPWTGTIPATHADRIRAAEASGRQAVQLATH
GPTPRDLLTPAAFRNALTVLQAIGGSTNGLVHLAAIARRAGLSVDLDAFDAIGRSVPVLIDLKPSGDHYMEHFHWAGGVP
KLMKELGDHLDRSAPTIDGRTIGEIADAAEEVPGQAIIRPASAPLKPFGGMAVLRGNLAPGGAVIKHSAASEALLRHTGR
AVVFDSVEDMVNRIDDPGLDVQADDVLVLRNAGPKGAPGMPEAGYLPIPKKLAQAGVKDMVRISDARMSGTAFGTIVLHI
TPEAAVGGPLGLVRSGDTIRLDVAAREIALLVPDEELERRRAEWTPPAHLAEAARGYQHLYLTHVLQADEGCDFDFC

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=584, Percent_Identity=32.1917808219178, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1786464, Length=496, Percent_Identity=31.0483870967742, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1788157, Length=517, Percent_Identity=30.7543520309478, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI2367371, Length=452, Percent_Identity=30.9734513274336, Blast_Score=159, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6322476, Length=554, Percent_Identity=32.6714801444043, Blast_Score=277, Evalue=3e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 59104; Mature: 59104

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00886 ILVD_EDD_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRNLASYGDPGFSLFLRKAFIKAAGYSDDALDRPIIGITNTFSDFNPCHGNVPRLIEAV
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHCCCCEEEECCCCCCCCCCCCHHHHHHHH
KRGVMLAGGLPMEFPTVSIHESFSNPTSMFLRNLMAMDTEEMIRAQPMDAVVLIGGCDKT
HCCEEEECCCCCCCCEEEHHHHCCCHHHHHHHHHHHCCHHHHHHCCCCCEEEEEECCCCC
VPAQLMGAVSANVPAIQLITGPMLVGHHKGEILGACTDCRRLWGQHRAGHLDAEEMDVLS
HHHHHHHHHHCCCCEEEEEECCEEEECCCCCEEHHHHHHHHHHHCCCCCCCCHHHHHHHH
GRLAPTQGTCMVMGTASTMGCLVEALGIALPWTGTIPATHADRIRAAEASGRQAVQLATH
CCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECC
GPTPRDLLTPAAFRNALTVLQAIGGSTNGLVHLAAIARRAGLSVDLDAFDAIGRSVPVLI
CCCCHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEECHHHHHHCCCCCCEEE
DLKPSGDHYMEHFHWAGGVPKLMKELGDHLDRSAPTIDGRTIGEIADAAEEVPGQAIIRP
EECCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEC
ASAPLKPFGGMAVLRGNLAPGGAVIKHSAASEALLRHTGRAVVFDSVEDMVNRIDDPGLD
CCCCCCCCCCEEEEECCCCCCCHHEEHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCC
VQADDVLVLRNAGPKGAPGMPEAGYLPIPKKLAQAGVKDMVRISDARMSGTAFGTIVLHI
CCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
TPEAAVGGPLGLVRSGDTIRLDVAAREIALLVPDEELERRRAEWTPPAHLAEAARGYQHL
CCCCCCCCCEEEEECCCEEEEEEEEEEEEEECCCHHHHHHHCCCCCHHHHHHHHCCHHHH
YLTHVLQADEGCDFDFC
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRRNLASYGDPGFSLFLRKAFIKAAGYSDDALDRPIIGITNTFSDFNPCHGNVPRLIEAV
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHCCCCEEEECCCCCCCCCCCCHHHHHHHH
KRGVMLAGGLPMEFPTVSIHESFSNPTSMFLRNLMAMDTEEMIRAQPMDAVVLIGGCDKT
HCCEEEECCCCCCCCEEEHHHHCCCHHHHHHHHHHHCCHHHHHHCCCCCEEEEEECCCCC
VPAQLMGAVSANVPAIQLITGPMLVGHHKGEILGACTDCRRLWGQHRAGHLDAEEMDVLS
HHHHHHHHHHCCCCEEEEEECCEEEECCCCCEEHHHHHHHHHHHCCCCCCCCHHHHHHHH
GRLAPTQGTCMVMGTASTMGCLVEALGIALPWTGTIPATHADRIRAAEASGRQAVQLATH
CCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECC
GPTPRDLLTPAAFRNALTVLQAIGGSTNGLVHLAAIARRAGLSVDLDAFDAIGRSVPVLI
CCCCHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEECHHHHHHCCCCCCEEE
DLKPSGDHYMEHFHWAGGVPKLMKELGDHLDRSAPTIDGRTIGEIADAAEEVPGQAIIRP
EECCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEC
ASAPLKPFGGMAVLRGNLAPGGAVIKHSAASEALLRHTGRAVVFDSVEDMVNRIDDPGLD
CCCCCCCCCCEEEEECCCCCCCHHEEHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCC
VQADDVLVLRNAGPKGAPGMPEAGYLPIPKKLAQAGVKDMVRISDARMSGTAFGTIVLHI
CCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
TPEAAVGGPLGLVRSGDTIRLDVAAREIALLVPDEELERRRAEWTPPAHLAEAARGYQHL
CCCCCCCCCEEEEECCCEEEEEEEEEEEEEECCCHHHHHHHCCCCCHHHHHHHHCCHHHH
YLTHVLQADEGCDFDFC
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]